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76 results about "Phytotron" patented technology

A phytotron is an enclosed research greenhouse used for studying interactions between plants and the environment. It was a product of the disciplines of plant physiology and botany.

Large scale multifunctional artificial climate room system for electrical test

The utility model relates to a large-scale multifunctional phytotron system for electrical tests, in particular to an artificial simulation test system with external insulation power transmission and transformation equipments in complex atmospheric environment, belonging to the high voltage and insulation technical field. The system comprises a phytotron body (1), a wall bushing (2) for leading-in the power supply, a wind speed simulation apparatus (3), a refrigeration system (4), an evacuation system (5), a steam fog system (6), a water treatment system (7), an ice coating, fog spraying and raining system (8), a hook and rotating apparatus (9) and a test environment measurement system (10). The large-scale multifunctional phytotron system of the utility model enables to simulate altitude of 232 to 7000m, atmospheric pressure of 98.6 to30KPa, temperature of -45 DEG C to 70 DEG C, wind speed of 0 to 12m / s and water electrical conductivity of 10 to 5000MuS / cm, and enables simulation and regulation of complex meteorological environment such as high altitude, ice coating, fog spraying, raining and other environments.
Owner:CHONGQING UNIV

Plant tissue culture environmental information monitoring and simulating system

InactiveCN103135540ARealize intelligent subordinateRealize wireless remote host controlPlant tissue cultureHorticulture methodsEngineeringPhytotron
The invention discloses a plant tissue culture environmental information monitoring and simulating system. The plant tissue culture environmental information monitoring and simulating system comprises a tissue culture box, a monitoring unit and a simulating unit, wherein the monitoring unit is formed by a collection module, a wireless transmission module and a display processing module. The collection module collects tissue culture environmental information of plants in the tissue culture box, and sends the environmental information to the transmission module. The wireless transmission module uploads the received environmental information to the display processing module. The display processing module stores, processes, displays and indexes the environmental information. The simulating unit carries out manual simulation on the tissue culture environmental information of the plants in the tissue culture box. The plant tissue culture environmental information monitoring and simulating system is convenient to operate, complete in functions, strong in pertinence, capable of achieving intelligent control for the information such as illumination, temperature, carbon dioxide (CO2) and the like in a greenhouse environment, and is suitable for greenhouse plant tissue culture and for various places such as plant tissue culture boxes, illumination culture boxes, phytotrons, facility agriculture, and has expansibility.
Owner:HEBEI UNIVERSITY

Phytotron haze environment simulating device and haze environment simulating method thereof

InactiveCN105115863ASame physical and chemical propertiesSimple test methodMaterial analysisParticulatesEngineering
The invention discloses a phytotron haze environment simulating device and a haze environment simulating method thereof. The device comprises a phytotron, a haze parameter detection unit, a cloud mist generation unit and a dust-haze generation unit, the haze parameter detection unit is arranged in the phytotron, and the output end of the cloud mist generation unit and the output end of the dust-haze generation unit are communicated with the interior of the phytotron. The simulating method comprises the steps that dust-haze particle raw materials are prepared and evenly mixed, the clout mist generation unit is started to form a cloud mist environment, the dust-haze generation unit is started to form a dust-haze environment, and mutual superposition, chemical combination and even mixing of mist steam and dust-haze particles are facilitated to form a haze environment. According to the phytotron haze environment simulating device and the haze environment simulating method thereof, PM2.5 particles and the stable haze environment of which the cloud mist concentration, chemical components of dust-haze particles and particle size distribution can be freely controlled and adjusted can be formed in the phytotron, and a safe and reliable experiment support is provided for researching the influence of the haze environment on human body health, outdoor traffic and electrical external insulation equipment.
Owner:STATE GRID CORP OF CHINA +2

Phytotron for feeding prodenia litura and indoor artificial breeding method

InactiveCN104206362ASuitable for growthFast Survival and GrowthAnimal husbandryFecesCataphyll
The invention discloses a phytotron for feeding prodenia litura and an indoor artificial breeding method. The method comprises the following steps: putting prodenia litura larvae into the phytotron under a specific condition; selecting natural hosts (tobacco leaf, Chinese cabbage and the like) to feed the prodenia litura larvae; changing fresh hosts everyday and removing faeces and rotten leaves; after the prodenia litura larvae pupate, centrally putting the prodenia litura into an insect rearing box; after adult emergence of the prodenia litura, pairing the prodenia litura according to the female-male ratio of 1 to 1, laying eggs and breeding population. According to the feeding method, the prodenia litura are fed in the manmade phytotron, the feeding technique is simple and easy to operate, low in feeding cost, and high in reproductive rate, and standard health test insects can be obtained in an indoor continuous feeding manner.
Owner:GUIZHOU UNIV

Miniature animal experiment phytotron and control method

The invention provides a miniature animal experiment phytotron which comprises a box body and a circuit controller, wherein the box body is divided into an animal feeding area, a temperature-humidity controlling area and an isolating cavity. The miniature animal experiment phytotron is characterized in that a temperature sensor, a humidity sensor, a gas pressure sensor, a gas composition sensor, an illuminance sensor, a wind speed sensor and a lighting lamp are arranged in the animal feeding area; an electric heating tube, a humidifier and refrigerating equipment are arranged in the temperature-humidity controlling area; a microcontroller is connected with a control circuit of the electric heating tube and the humidifier through a solid-state relay, connected with a control circuit of the lighting lamp through a digital rheostat, and connected with an exhaust blower and a blast blower through an optical-isolating silicon controlled rectifier driving circuit. By adopting the miniature animal experiment phytotron, climate parameters comprising the temperature, the humidity, gas pressure, gas compositions, the illuminance and the wind speed in the feeding area can be measured and regulated, wherein the temperature can be regulated in the range of 0 DEG to 50 DEG, the humidity can be regulated in the range of 30 percent relative humidity (RH) to 90 percent RH and the wind speed can be regulated in the range of 0.5m / s to 5m / s, so as to overcome the defect of less or single parameter of an simulated environment of the prior phytotron.
Owner:SHANDONG LVDU BIO SICIENCE & TECH +1

Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution

The invention relates to a method for reducing cadmium content of rice seedling leaves, and a rice seedling nutrient solution recipe. The method comprises: (1) rice variety being Yangdao 6; (2) sowing seeds whose surfaces are disinfected to plugs which use vermiculite as medium, putting the plugs in a phytotron to culture for 1-2 weeks; (3) selecting rice seedlings to transplant in cultivating pots, the nutrient solution used for water planting being international rice nutrient solution and being added with microelements. Through discussing influence of external Fe and Mn on formation of rice seedling root iron plaque and on Cd absorption and transport and on rice seedling antioxidant enzyme activities, the method provides new thoughts for rice root iron plaque research and rice safety production on Cd polluted fields.
Owner:AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI

Rapid seedling method of fruit tree hybrid seeds

The invention discloses a seedling method and particularly relates to a rapid seedling method for fruit tree hybrid seeds. The rapid seedling method includes after the fruits are collected, removing pulps and taking out seeds without shells, placing the seeds in culture dishes, pouring sterile water with 20-25 degrees in the culture dishes, and soaking the seeds in the sterile water within 24-36hs; removing seed coats, sterilizing the seeds without coats with 0.08%-0.12% potassium permanganate solution for 24-36secs, and placing the seeds waking up from dormancy in the container filled with the sterile water for seeding; seeding the seeds waking up from the dormancy in watered matrixes for culture in a phytotron; after the seeds grow the fifth or the sixth leaves, removing the seeds out of the phytotron and culturing the same under the indoor temperature for 2-3 days for water controlling, and then transplanting the seeds to feeding blocks or fields to grow. By the application of the rapid seedling method, seed development period is shortened, and emergence rate of hybrid seeds is increased.
Owner:辽宁省果树科学研究所

Pupal-stage parasitic wasp artificial-propagation method, wasp-keeping box and manufacturing method of wasp-keeping box

ActiveCN103918613AQuick changeReplace batchAnimal husbandryCold airZoology
The invention relates to a pupal-stage parasitic wasp artificial-propagation method, a wasp-keeping box and the manufacturing method of the wasp-keeping box. The pupal-stage parasitic wasp artificial-propagation method comprises the steps of (1) placing the parasitic wasp keeping box into a phytotron or a man-made climate box and placing parasitic wasps and hosts into the wasp-keeping box, (2) feeding the parasitic wasps periodically and guaranteeing the availability of food and water for the parasitic wasps in the box, (3) collecting and replacing the hosts, (4) supplementing parasitic wasps in the wasp-keeping box periodically and feeding the collected parasitized hosts, and (5) storing the parasitized hosts when the parasitic wasps grow to the pupal stage. By the adoption of the novel method that artificial propagation is conducted in a self-made wasp-keeping box device, people only need to open a spiral cover at the position of an inlet and use a blower to blow cold air to achieve parasitic wasp adding or host replacement, host replacement can be conducted fast in batches, and wasp propagation efficiency is improved.
Owner:ANHUI NORMAL UNIV

Method for identifying stability of cadmium accumulation environment of grains of rice varieties

The invention discloses a method for identifying stability of cadmium accumulation environment of the grains of rice varieties. The method includes planting paddy rice s of a variety in a pot, controlling soil moisture through a jardiniere, controlling air temperature through a phytotron, carrying out soil water content and air temperature treatment each for an active tillering stage, a heading period and a grain filling stage of the rice variety, harvesting paddy rice seeds according to the water contents and temperature processed and unprocessed contrast sets after the paddy rice matures, and detecting the cadmium content of the polished rice. If the cadmium content of the polished rice is lower than natural standard of 0.2mg / kg, the rice variety has stable grains and is low in cadmium accumulation under similar cadmium pollution level. The method overcomes the defects that a conventional means for sorting low cadmium rice variety in natural field environment is poor in repeatability annually, Paddy rice varieties with low cadmium can be identified, with key cadmium absorption and fertility stages being not affected by lowering precipitation and sharp rise of temperature. The method makes contribution to agricultural grain safety and protection of people's health.
Owner:HUNAN RICE RES INST +2

Factory-like bagging cultivation method for agrocybe cylindracea

The invention relates to the technical field of culturing of edible mushrooms, in particular to a factory-like bagging cultivation method for agrocybe cylindracea. The method includes the following steps that (1) an agrocybe cylindracea culture medium is prepared; (2) bagging is performed; (3) sterilization is performed; (4) liquid strains are inoculated; (5) mycelium culture is performed, the temperature of a culturing room is set in a range from 21 DEG C to 25 DEG C, and the humidity of the culturing room is set in a range from 70% to 80%; (6) during management of producing mushroom, scattering illumination is maintained in a phytotron, the temperature is maintained in a range from 10 DEG C to 15 DEG C 3-5 days earlier and then the temperature is increased in a range from 22 DEG C to 25 DEG C for 3-5 days, or the temperature is maintained in a range from 22 DEG C to 25 DEG C 3-5 days earlier and then the temperature is decreased in a range from 10 DEG C to 15 DEG C for 3-5 days. By means of the factory-like bagging cultivation method for agrocybe cylindracea, the producing efficiency and the yield are high, manpower and production costs can be lowered, and the cultivated agrocybe cylindracea has the advantages of short growth cycle, fast growth of mycelia and sporocarp, high yield and thick and firm agrocybe cylindracea.
Owner:曾令钢

An indoor plant growth artificial climate chamber that fully utilizes sunlight

The invention discloses an indoor plant growth artificial climate chamber which fully utilizes sunlight. It includes light collectors, sunlight detectors, drive motors, optical fibers, sunlight distributors, supplementary lights, climate chamber enclosures, return air orifice plates, air conditioning units; light collectors, sunlight detection The controller and driving motor are installed above the exterior of the climate chamber enclosure structure, and the exterior of the climate chamber enclosure structure is also equipped with an air conditioning unit. The solar distributor and supplementary light are evenly distributed on the top of the climate chamber enclosure structure. Both sides of the enclosure structure of the room are equipped with return air orifice plates, and the light collector is connected with the sunlight detector and the driving motor at the same time, and is connected with the sunlight distributor through the optical fiber. The invention can accurately control various environmental parameters in a light-tight airtight space, and realizes plant cultivation experiments using sunlight as a growth light source.
Owner:HANGZHOU QIUSHI ARTIFICIAL ENVIRONMENT

Tobacco black shank resistance single plant identification method

The invention discloses a tobacco black shank resistance single plant identification method. The method includes the steps that tobacco which is about to be inoculated and grows orderly is obtained through tissue culturing rapid propagation, application inoculation processing is conducted, culture medium slab patches overgrown with black shank hyphae are attached to the rootstocks of microtrauma tobacco, the treated tobacco is cultivated in a humidity-controllable phytotron, resistance distinguish is conducted according to a research result generated 14 days after inoculation is conducted, and the resistance is divided according to the morbidity a. The immune or highly-resistant morbidity a is equal to zero, the disease-resistant morbidity a is larger than zero and smaller than or equal to 25%, the moderately-resistant morbidity a is larger than 25% and smaller than or equal to 45%, the moderately-susceptible morbidity a is larger than 45% and smaller than or equal to 65%, the susceptible morbidity a is larger than 65% and smaller than or equal to 80%, the highly-susceptible morbidity a is larger than 80% and smaller than or equal to 100%, and the immune or highly-resistant, disease-resistant and moderately-resistant materials are resistance materials. The method is high in identification result reproducibility, good in stability, high in efficiency, capable of achieving large-scale resistance identification and especially suitable for screening disease-resistant target single plants from a huge number of mutagenesis populations or mutant population, hybridization variation populations.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI

Field phytotron temperature control system and temperature control method

The invention belongs to the field of artificial environment control of constant-temperature and constant-humidity laboratories, phytotrons and the like and relates to a field phytotron temperature control system and temperature control method. The system comprises a constant-temperature water tank, a water chilling unit, a mold temperature controller, a first air heat exchanger and a second air heat exchanger; a water outlet of the constant-temperature water tank is communicated with a water inlet of the water chilling unit through a water pipe. By means of the design, the temperature of a phytotron can be accurately controlled, the crop growth climate environment under the natural condition can be simulated according to experiment requirements, and the problems of large air temperature control error, large indoor temperature uniformity error, large environment humidity change and high energy consumption of a phytotron are solved.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI +1

An evaluating method of cotton variety saline tolerance

InactiveCN105353089AAvoid Weights Adopt Expert EmpowermentAvoid the pitfalls of equal rightsCultivating equipmentsPlant cultivationNutritionStatistical analysis
An evaluating method of cotton variety saline tolerance is disclosed. The method includes S1) a step of cultivating seedlings, namely a step of cultivating cotton seedlings until leafing by using a substrate manner for seedling cultivation; S2) a step of transplanting, namely a step of transplanting cotton plants with similar growing vigour into a planting cup containing fine sand and having a hole at the bottom, putting the planting cup into a container, adding a nutrition liquid, and cultivating in a phytotron; S3) a step of detecting indexes, wherein a NaCl-free nutrition liquid is adopted as a contrast, a NaCl nutrition liquid having a concentration of 150 mmol.L<-1> for salt stress treatment is adopted as a treatment group, the nutrition liquid of each of the two groups is changed every four days, nine plants are selected from each of the groups in the ninth day, and related indexes are detected; S4) a step of statistic analysis of data, and other steps. According to the method, agronomic character indexes and physiological and biochemical indexes are considered comprehensively, limitation of artificially assigned weights for each target character is overcome, and objective and effective evaluation for variety saline tolerance is achieved.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Simulated phytotron for Arma chinensis breeding

The invention discloses a simulated phytotron for Arma chinensis breeding, comprising a house body which is closed inside. A Mythimna separata larva raising device, an Arma chinensis larva breeding device, an Arma chinensis adult feeding device, a temperature control device and a humidity control device are arranged in the house body. The simulated phytotorn aims to solve the problem of low survival rate of Arma chinensis in artificial raising in the prior art.
Owner:GUIZHOU TOBACCO CO LTD QIANDONGNAN AUTONOMOUS PREFECTURE BRANCH

Crop phytotron capable of accurately and uniformly controlling temperature of cyclone airflow

The invention provides a crop phytotron capable of accurately and uniformly controlling temperature of cyclone airflow. According to the crop phytotron capable of accurately and uniformly controllingthe temperature of cyclone airflow, at least two air supply ports, two air return ports and two auxiliary fans are diagonally arranged to form a cyclone airflow field of the phytotron. According to the temperature control, two-stage heat balance and two-variable temperature control measures are adopted: in the first-stage energy balance, cold water and hot water are prepared firstly; and in the second stage, cold water or hot water is used for balancing the heat exchange capacity between the phytotron and the outside. Two variables for adjusting energy are the water inlet flow and the water inlet temperature of an air heat exchanger. According to the crop phytotron, a temperature control model of the crop phytotron is derived, and a theoretical basis is provided for accurate temperature control of the crop phytotron; three signals need to be utilized for temperature control are provided and include solar radiation, enclosure structure heat transfer flow and indoor temperature, and corresponding temperature difference values are provided; embodiments are described; and the innovation of the theory and the technology has important significance and practical value.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI +1

Wire icing thickness measurement and equivalent conversion method in phytotron

ActiveCN104949642AQuick survey workQuick calculation jobMeasurement devicesPhytotronPhysics
The invention discloses a wire icing thickness measurement and equivalent conversion method in a phytotron. The method comprises steps as follows: (1) calculating the radius of a wire and measuring the quality and the length of the wire; (2) performing icing on the wire; (3) measuring and converting the icing quality of the wire; (4) calculating the icing volume of the wire; (5) calculating the icing thickness of the wire; (6) repeating steps (2) to (5) and continuously calculating the icing thickness of the wire. With the adoption of the method, the icing thickness of the wire can be measured quickly and continuously, and the measuring method is simple, efficient and easy to implement.
Owner:STATE GRID CORP OF CHINA +2

Artificial climate chamber of built-in temperature controlling circulating system

The invention provides a phytotron with a built-in temperature control circulation system, which is used for determining the germination rate of crop seeds, culturing seedlings and fungi. The phytotron consists of a phytotron box body, a circulation air duct, heating-cooling heat transfer devices, a blower, a return air inlet and air outlets, and is characterized in that the inner side of a ceiling is provided with an interlayer; an inner wall surface and inner wall corners are respectively provided with vertical air ducts; the ceiling interlayer is communicated respectively with the vertical air ducts so as to form the circulation air duct; the return air inlet in the center of the ceiling interlayer is provided with a return air inlet blower; the air outlets around the return air inlet blower are provided with the heating-cooling heat transfer devices; and a plurality of air outlets and air outlet blowers are arranged on the surfaces of the vertical air ducts. The phytotron has the advantages that the phytotron can improve the precision and evenness of indoor temperature control, prevent culture mediums and seedlings from being blow-dried by strong wind, and ensure tidy regular indoor and outdoor geometric shapes.
Owner:崔学晨 +2

Molecular seed breeding method by using single-fragment substitution line for pyramid-improving heat resistance during heading and flowering stage and filling stage of paddy rice

InactiveCN106947802AIncreased heat resistance at the heading and flowering stageMicrobiological testing/measurementPlant genotype modificationTemperature stressAgricultural science
The invention belongs to molecular seed breeding and relates to a molecular seed breeding method for breeding a new paddy rice variety which has heat resistance during heading and flowering stage and filling stage. The method includes the steps of: 1) with a mid-indica two-line elite restorer line 9311, which is assigned the accession number of CGMCC No.12532, as a female parent, and an F1 generation, which is obtained by hybridizing Yuanjiang common wild rice single-fragment substitution lines YJ07-04-06 and YJ01-05-03 assigned the accession numbers of CGMCC No.12534 and CGMCC No.12535, as a male parent, performing intercross for 1 generation, performing backcross for 3 generations with a recurrent parent, and then performing selfing for 3 generations to obtain a stable homozygous plant strain BC3F4, wherein each generation is harvested in a single spike manner; and 2) identifying and selecting a new paddy rice variety by means of molecule marker assisted selection with combination of phytotron for simulating high temperature stress. By means of the method, a new paddy rice variety "Yuanye No.3" having heat resistance during the heading and flowering stage and filling stage has been cultured. Target characters are selected through molecule marker assistance, so that a restorer line having heat resistance during the heading and flowering stage and filling stage can be cultured within 3-4 years quickly and high-effectively.
Owner:JIANGXI ACAD OF AGRI SCI

Young shoot grafting method for virus removal of apples

InactiveCN108076878ASolve the problem of low survival rate of graftingImprove the survival rate of graftingGraftingShootMain stem
The invention provides a young shoot grafting method for virus removal of apples. The method comprises the following steps: disinfecting grafting tools in advance; detecting virus carrying conditionsof leaves located at 4-5cm the top of a new shoot which is grown to 15-20cm and treated by virus removal, retaining 1-2 young leaves on the top end of the new shoot of which target virus is removed, and cutting a shoot tip with the length of 3-4cm to be used as a scion; selecting a potted virus-free annual growth rootstock seedling with the main stem diameter of 1.5cm or more to be used as a virus-free rootstock, performing rootstock cutting at the height of 25-35cm far away from the ground; and after grafting is performed, performing moisturizing by performing bagging on the shoot tip and a connection opening part, performing moisturizing culture in a phytotron for 10-15 days, and performing seedling exercising to obtain a virus-free seedling of apples. According to the method provided bythe invention, shoot tips obtained by special ways such as an insufficient maturity degree, seasonal limitation, tissue culture, and anti-seasonal seed introduction can perform apple seedling grafting, preservation and reproduction at any time, the problem of a low survival rate of young shoot grafting in a conventional grafting method is solved, and a novel method is opened up for reproduction of virus-free seedlings.
Owner:北京市林业果树科学研究院 +1

Outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method and application thereof

The invention discloses an outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method. The method includes the steps that natural inoculation and feeding are conducted on tested insects; supplied test fungicides are prepared into liquid of a set concentration; branches with insects of potted nursery stock are integrally soaked into the prepared liquid, and then the soaked branches with insects are enveloped by a thin and soft gauze element; the potted nursery stock which is soaked with pesticide is placed in a phytotron with the light dark period being L:D=14:10 h, the temperature being 25+ / -1 DEG C and the relative humidity being 75% to be fed; the death and survival condition of the tested icerya purchasi is examined after the icerya purchasi is fed for 24 hours, and the death number and the survival number are recorded; a living body plant is used for cultivating the tested insects which are soaked with the liquid, the food freshness of the icerya purchasi is guaranteed, sufficient nutrients are provided for the icerya purchasi, testing is conducted under a natural environment growth condition where the icerya purchasi is placed, the accuracy of biological assay results is effectively improved, hence, the icerya purchasi population resistance level can be known comprehensively, accurately and precisely, and a basis is provided for reasonably utilizing pesticide and making a resistance treatment plan accurately.
Owner:CITRUS RES INST OF CHINESE ACAD OF AGRI SCI

Determination method for CO2 feeding amount of greenhouse culture green vegetable plants

The invention provides a determination method for the CO2 feeding amount of greenhouse culture green vegetable plants. The method comprises the steps of 1, photosynthetic tester selection and adjusting; 2, preparation of a small phytotron; 3, culture and preparation of to-be-tested plants; 4, manufacturing of a CO2 constant chamber; 5, measurement setting; 6, determination of net photosynthetic rates under different temperatures; 7, determination of net photosynthetic rates under different illumination intensities; 8, determination of net photosynthetic rates under different temperature and illumination intensity combinations; 9, fitting of equations; 10, application. Due to the data collection, computer technology application and other modes, the artificial feeding CO2 time and consumption are determined, CO2 consumed by plant use in a greenhouse can be supplemented in time in production, and the scientific basis is provided for increasing the greenhouse culture green vegetable yield;the method has the advantages of being low in cost, easy and convenient to operate and remarkable in effect.
Owner:SHANGHAI INSTITUTE OF TECHNOLOGY

Icing shape test-based overhead power transmission line galloping prediction method and system

The invention relates to the technical field of power transmission and distribution, and discloses an icing shape test-based overhead power transmission line galloping prediction method and system, which gets rid of constraints of a field detection device and realizes scientific and reliable line galloping prediction. The method comprises the steps of carrying out icing shape tests of different line structures under different meteorological conditions in a phytotron to obtain observation records; according to the observation records of the phytotron, drawing an icing thickness increment curve, and through curve fitting, establishing a function relationship of icing thickness increment degree; determining an icing non-uniformity calculation formula, drawing an icing non-uniformity curve according to the observation records of the phytotron, and through curve fitting, establishing a function relationship of icing shape non-uniformity; and according to the fit icing thickness increment degree function, predicting icing thickness in a natural condition, according to the fit icing shape non-uniformity function, predicting icing non-uniformity in the natural condition, and based on a galloping prediction model, performing power transmission line galloping prediction.
Owner:STATE GRID HUNAN ELECTRIC POWER +3

Breeding method of excellent novel germplasm of soybeans

The invention provides a breeding method of an excellent novel germplasm of soybeans. In the method, recurrent selection is carried out by using a male genic sterile gene, the defects of artificial castration hybridization are completely overcome, and the randomness of cross parent selection and crossbreeding efficiency are greatly improved. The recurrent selection technology of the male genic sterile gene is introduced into group hybridization, intercross, backcross and composite cross plans of soybean germplasm, through hybridized combination prepared in a summer net chamber, off-season multiplication for generation addition in winter and secondary generation addition in an artificial phytotron in spring, three generations are realized each year, marker-assisted selection is used, largebatches and multiple types of novel germplasm materials of soybeans can be rapidly obtained by non-destructive quality detection, indoor resistance identification and multi-point adaptability identification, precious and excellent parents are provided for breeding of new varieties, and soybean breeding has infinite potentials.
Owner:陕西省杂交油菜研究中心

Molecular seed breeding method by using single-fragment substitution line for improving heat resistance during heading and flowering stage of paddy rice

InactiveCN106947803AIncreased heat resistance at the heading and flowering stageMicrobiological testing/measurementPlant genotype modificationTemperature stressMarker-assisted selection
The invention belongs to the field of molecular seed breeding and relates to a molecular seed breeding method for breeding a new paddy rice variety which has heat resistance during heading and flowering stage. The method includes the steps of: 1) with a mid-indica two-line elite restorer line 9311, which is assigned the accession number of CGMCC No.12532, as a female parent, and a wild rice chromosome fragment introgression line YJ07-04-06, which is assigned the accession number of CGMCC No.12534, as a male parent, performing hybridization for 1 generation and backcross for 3 generations, and then performing selfing for 3 generations to obtain BC3F4, wherein each generation is harvested in a single plant manner; 2) identifying and selecting a new paddy rice variety by means of molecule marker assisted selection with combination of phytotron for simulating high temperature stress. By means of the method, a new paddy rice variety "Yuanye No.2" having heat resistance during the heading and flowering stage has been cultured. Target characters are selected through molecule marker assistance, so that a restorer line having heat resistance during the heading and flowering stage can be cultured within 3-4 years quickly and high-effectively.
Owner:JIANGXI ACAD OF AGRI SCI

A kind of hydroponic multiplication method of tissue culture seedlings

The invention relates to a method for hydroponic proliferation of tissue culture seedlings of thesium chinense Turcz.. The method comprises the steps of firstly, taking strong terminal buds of thesiumchinense Turcz., cleaning and disinfecting the terminal buds, and then inoculating the terminal buds into an MS culture medium for culture; secondly, transferring the terminal buds into a cluster budinduction culture medium for culture, inoculating generated adventitious buds into a proliferation culture medium for seedling strengthening proliferation culture, and obtaining the tissue culture seedlings of thesium chinense Turcz.; selecting the strong tissue culture seedlings dark green in color, transferring the tissue culture seedlings into an artificial climate chamber, opening a cover forhardening of the seedlings to obtain hydroponic mother seedlings of thesium chinense Turcz., planting the hydroponic mother seedlings into a hydroponic tank filled with a proliferation nutrient solution, and performing hydroponic proliferation culture to obtain the proliferated tissue culture rootless seedlings of thesium chinense Turcz.. The hydroponic seedlings obtained by means of the method have good quality and can be spread flat for air-drying for 2 days to serve as a raw material for extraction of kaempferol 3-glucorhamnoside, extraction of kaempferol 3-glucorhamnoside on the basis oftissue culture seedlings and wild herba thesium chinense Turcz. can be completely replaced, and the problem of resource shortage of thesium chinense Turcz. is solved. Compared with existing rootless seedling proliferation technologies of thesium chinense Turcz., the method has the advantages of large proliferation coefficient and low production cost, and large-scale production of the tissue culture rootless seedlings of thesium chinense Turcz. is facilitated.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for transforming in-vitro soybean growing points with gene gun

The invention discloses a method for transforming in-vitro soybean growing points with a gene gun, belonging to the technical field of bioengineering. The method is characterized by making bullets with carriers containing target genes, loading the bullets into the gene gun, then bombarding aseptic seedlings and performing inoculation induction to obtain T0-generation plants; performing rooting induction and seedling hardening by opening a vessel lid on the T0-generation plants, and then inducing the plants to bloom and fruit to obtain T1-generation seeds; and finally transplanting the T1-generation seeds to a phytotron or large fields to obtain T2-generation plants and homozygous positive transformant thereof. The method has the following advantage: by utilizing the characteristic that the plants prepared from the in-vitro soybean growing points are relatively easy to regenerate, the transformation efficiency can be obviously improved.
Owner:上海交通大学中原研究院

Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling

The invention relates to the technical field of light regulation and control, in particular to a molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling. According to the method, during an experiment, excellent pakchoi seeds are firstly obtained, cultivation experiment work is carried out, finally obtained experiment data are more accurate, the credibility of the experiment is improved, then the seeds are placed in sterile water to be soaked, the seeds can rapidly absorb water, the germination time of the seeds is shortened, meanwhile, sterile water is used for soaking the seeds, the influence of bacteria on the seeds can be reduced when the seeds absorb water, the survival rate of the seeds is increased, meanwhile, the temperature and humidity of a seedling chamber and a phytotron are manually adjusted, the temperature and humidity are manually adjusted to be suitable for the growth of the pakchoi, the growth speed of the pakchoi is increased, during growth, UV-A coupling and blue light with different light intensities are used for light regulation and control work, and the anthocyanin component rich in the pakchoi is increased along with the change of time.
Owner:INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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