76 results about "Phytotron" patented technology

The utility model relates to a large-scale multifunctional phytotron system for electrical tests, in particular to an artificial simulation test system with external insulation power transmission and transformation equipments in complex atmospheric environment, belonging to the high voltage and insulation technical field. The system comprises a phytotron body (1), a wall bushing (2) for leading-in the power supply, a wind speed simulation apparatus (3), a refrigeration system (4), an evacuation system (5), a steam fog system (6), a water treatment system (7), an ice coating, fog spraying and raining system (8), a hook and rotating apparatus (9) and a test environment measurement system (10). The large-scale multifunctional phytotron system of the utility model enables to simulate altitude of 232 to 7000m, atmospheric pressure of 98.6 to30KPa, temperature of -45 DEG C to 70 DEG C, wind speed of 0 to 12m/s and water electrical conductivity of 10 to 5000MuS/cm, and enables simulation and regulation of complex meteorological environment such as high altitude, ice coating, fog spraying, raining and other environments.

The invention discloses a plant tissue culture environmental information monitoring and simulating system. The plant tissue culture environmental information monitoring and simulating system comprises a tissue culture box, a monitoring unit and a simulating unit, wherein the monitoring unit is formed by a collection module, a wireless transmission module and a display processing module. The collection module collects tissue culture environmental information of plants in the tissue culture box, and sends the environmental information to the transmission module. The wireless transmission module uploads the received environmental information to the display processing module. The display processing module stores, processes, displays and indexes the environmental information. The simulating unit carries out manual simulation on the tissue culture environmental information of the plants in the tissue culture box. The plant tissue culture environmental information monitoring and simulating system is convenient to operate, complete in functions, strong in pertinence, capable of achieving intelligent control for the information such as illumination, temperature, carbon dioxide (CO2) and the like in a greenhouse environment, and is suitable for greenhouse plant tissue culture and for various places such as plant tissue culture boxes, illumination culture boxes, phytotrons, facility agriculture, and has expansibility.

The invention discloses a phytotron haze environment simulating device and a haze environment simulating method thereof. The device comprises a phytotron, a haze parameter detection unit, a cloud mist generation unit and a dust-haze generation unit, the haze parameter detection unit is arranged in the phytotron, and the output end of the cloud mist generation unit and the output end of the dust-haze generation unit are communicated with the interior of the phytotron. The simulating method comprises the steps that dust-haze particle raw materials are prepared and evenly mixed, the clout mist generation unit is started to form a cloud mist environment, the dust-haze generation unit is started to form a dust-haze environment, and mutual superposition, chemical combination and even mixing of mist steam and dust-haze particles are facilitated to form a haze environment. According to the phytotron haze environment simulating device and the haze environment simulating method thereof, PM2.5 particles and the stable haze environment of which the cloud mist concentration, chemical components of dust-haze particles and particle size distribution can be freely controlled and adjusted can be formed in the phytotron, and a safe and reliable experiment support is provided for researching the influence of the haze environment on human body health, outdoor traffic and electrical external insulation equipment.

The invention discloses a phytotron for feeding prodenia litura and an indoor artificial breeding method. The method comprises the following steps: putting prodenia litura larvae into the phytotron under a specific condition; selecting natural hosts (tobacco leaf, Chinese cabbage and the like) to feed the prodenia litura larvae; changing fresh hosts everyday and removing faeces and rotten leaves; after the prodenia litura larvae pupate, centrally putting the prodenia litura into an insect rearing box; after adult emergence of the prodenia litura, pairing the prodenia litura according to the female-male ratio of 1 to 1, laying eggs and breeding population. According to the feeding method, the prodenia litura are fed in the manmade phytotron, the feeding technique is simple and easy to operate, low in feeding cost, and high in reproductive rate, and standard health test insects can be obtained in an indoor continuous feeding manner.

The invention relates to a method for reducing cadmium content of rice seedling leaves, and a rice seedling nutrient solution recipe. The method comprises: (1) rice variety being Yangdao 6; (2) sowing seeds whose surfaces are disinfected to plugs which use vermiculite as medium, putting the plugs in a phytotron to culture for 1-2 weeks; (3) selecting rice seedlings to transplant in cultivating pots, the nutrient solution used for water planting being international rice nutrient solution and being added with microelements. Through discussing influence of external Fe and Mn on formation of rice seedling root iron plaque and on Cd absorption and transport and on rice seedling antioxidant enzyme activities, the method provides new thoughts for rice root iron plaque research and rice safety production on Cd polluted fields.

The invention relates to a pupal-stage parasitic wasp artificial-propagation method, a wasp-keeping box and the manufacturing method of the wasp-keeping box. The pupal-stage parasitic wasp artificial-propagation method comprises the steps of (1) placing the parasitic wasp keeping box into a phytotron or a man-made climate box and placing parasitic wasps and hosts into the wasp-keeping box, (2) feeding the parasitic wasps periodically and guaranteeing the availability of food and water for the parasitic wasps in the box, (3) collecting and replacing the hosts, (4) supplementing parasitic wasps in the wasp-keeping box periodically and feeding the collected parasitized hosts, and (5) storing the parasitized hosts when the parasitic wasps grow to the pupal stage. By the adoption of the novel method that artificial propagation is conducted in a self-made wasp-keeping box device, people only need to open a spiral cover at the position of an inlet and use a blower to blow cold air to achieve parasitic wasp adding or host replacement, host replacement can be conducted fast in batches, and wasp propagation efficiency is improved.

The invention discloses a method for identifying stability of cadmium accumulation environment of the grains of rice varieties. The method includes planting paddy rice s of a variety in a pot, controlling soil moisture through a jardiniere, controlling air temperature through a phytotron, carrying out soil water content and air temperature treatment each for an active tillering stage, a heading period and a grain filling stage of the rice variety, harvesting paddy rice seeds according to the water contents and temperature processed and unprocessed contrast sets after the paddy rice matures, and detecting the cadmium content of the polished rice. If the cadmium content of the polished rice is lower than natural standard of 0.2mg/kg, the rice variety has stable grains and is low in cadmium accumulation under similar cadmium pollution level. The method overcomes the defects that a conventional means for sorting low cadmium rice variety in natural field environment is poor in repeatability annually, Paddy rice varieties with low cadmium can be identified, with key cadmium absorption and fertility stages being not affected by lowering precipitation and sharp rise of temperature. The method makes contribution to agricultural grain safety and protection of people's health.

The invention relates to the technical field of culturing of edible mushrooms, in particular to a factory-like bagging cultivation method for agrocybe cylindracea. The method includes the following steps that (1) an agrocybe cylindracea culture medium is prepared; (2) bagging is performed; (3) sterilization is performed; (4) liquid strains are inoculated; (5) mycelium culture is performed, the temperature of a culturing room is set in a range from 21 DEG C to 25 DEG C, and the humidity of the culturing room is set in a range from 70% to 80%; (6) during management of producing mushroom, scattering illumination is maintained in a phytotron, the temperature is maintained in a range from 10 DEG C to 15 DEG C 3-5 days earlier and then the temperature is increased in a range from 22 DEG C to 25 DEG C for 3-5 days, or the temperature is maintained in a range from 22 DEG C to 25 DEG C 3-5 days earlier and then the temperature is decreased in a range from 10 DEG C to 15 DEG C for 3-5 days. By means of the factory-like bagging cultivation method for agrocybe cylindracea, the producing efficiency and the yield are high, manpower and production costs can be lowered, and the cultivated agrocybe cylindracea has the advantages of short growth cycle, fast growth of mycelia and sporocarp, high yield and thick and firm agrocybe cylindracea.

The invention discloses an indoor plant growth artificial climate chamber which fully utilizes sunlight. It includes light collectors, sunlight detectors, drive motors, optical fibers, sunlight distributors, supplementary lights, climate chamber enclosures, return air orifice plates, air conditioning units; light collectors, sunlight detection The controller and driving motor are installed above the exterior of the climate chamber enclosure structure, and the exterior of the climate chamber enclosure structure is also equipped with an air conditioning unit. The solar distributor and supplementary light are evenly distributed on the top of the climate chamber enclosure structure. Both sides of the enclosure structure of the room are equipped with return air orifice plates, and the light collector is connected with the sunlight detector and the driving motor at the same time, and is connected with the sunlight distributor through the optical fiber. The invention can accurately control various environmental parameters in a light-tight airtight space, and realizes plant cultivation experiments using sunlight as a growth light source.

The invention discloses a tobacco black shank resistance single plant identification method. The method includes the steps that tobacco which is about to be inoculated and grows orderly is obtained through tissue culturing rapid propagation, application inoculation processing is conducted, culture medium slab patches overgrown with black shank hyphae are attached to the rootstocks of microtrauma tobacco, the treated tobacco is cultivated in a humidity-controllable phytotron, resistance distinguish is conducted according to a research result generated 14 days after inoculation is conducted, and the resistance is divided according to the morbidity a. The immune or highly-resistant morbidity a is equal to zero, the disease-resistant morbidity a is larger than zero and smaller than or equal to 25%, the moderately-resistant morbidity a is larger than 25% and smaller than or equal to 45%, the moderately-susceptible morbidity a is larger than 45% and smaller than or equal to 65%, the susceptible morbidity a is larger than 65% and smaller than or equal to 80%, the highly-susceptible morbidity a is larger than 80% and smaller than or equal to 100%, and the immune or highly-resistant, disease-resistant and moderately-resistant materials are resistance materials. The method is high in identification result reproducibility, good in stability, high in efficiency, capable of achieving large-scale resistance identification and especially suitable for screening disease-resistant target single plants from a huge number of mutagenesis populations or mutant population, hybridization variation populations.

An evaluating method of cotton variety saline tolerance is disclosed. The method includes S1) a step of cultivating seedlings, namely a step of cultivating cotton seedlings until leafing by using a substrate manner for seedling cultivation; S2) a step of transplanting, namely a step of transplanting cotton plants with similar growing vigour into a planting cup containing fine sand and having a hole at the bottom, putting the planting cup into a container, adding a nutrition liquid, and cultivating in a phytotron; S3) a step of detecting indexes, wherein a NaCl-free nutrition liquid is adopted as a contrast, a NaCl nutrition liquid having a concentration of 150 mmol.L<-1> for salt stress treatment is adopted as a treatment group, the nutrition liquid of each of the two groups is changed every four days, nine plants are selected from each of the groups in the ninth day, and related indexes are detected; S4) a step of statistic analysis of data, and other steps. According to the method, agronomic character indexes and physiological and biochemical indexes are considered comprehensively, limitation of artificially assigned weights for each target character is overcome, and objective and effective evaluation for variety saline tolerance is achieved.

The invention provides a crop phytotron capable of accurately and uniformly controlling temperature of cyclone airflow. According to the crop phytotron capable of accurately and uniformly controllingthe temperature of cyclone airflow, at least two air supply ports, two air return ports and two auxiliary fans are diagonally arranged to form a cyclone airflow field of the phytotron. According to the temperature control, two-stage heat balance and two-variable temperature control measures are adopted: in the first-stage energy balance, cold water and hot water are prepared firstly; and in the second stage, cold water or hot water is used for balancing the heat exchange capacity between the phytotron and the outside. Two variables for adjusting energy are the water inlet flow and the water inlet temperature of an air heat exchanger. According to the crop phytotron, a temperature control model of the crop phytotron is derived, and a theoretical basis is provided for accurate temperature control of the crop phytotron; three signals need to be utilized for temperature control are provided and include solar radiation, enclosure structure heat transfer flow and indoor temperature, and corresponding temperature difference values are provided; embodiments are described; and the innovation of the theory and the technology has important significance and practical value.

The invention discloses a wire icing thickness measurement and equivalent conversion method in a phytotron. The method comprises steps as follows: (1) calculating the radius of a wire and measuring the quality and the length of the wire; (2) performing icing on the wire; (3) measuring and converting the icing quality of the wire; (4) calculating the icing volume of the wire; (5) calculating the icing thickness of the wire; (6) repeating steps (2) to (5) and continuously calculating the icing thickness of the wire. With the adoption of the method, the icing thickness of the wire can be measured quickly and continuously, and the measuring method is simple, efficient and easy to implement.

The invention provides a phytotron with a built-in temperature control circulation system, which is used for determining the germination rate of crop seeds, culturing seedlings and fungi. The phytotron consists of a phytotron box body, a circulation air duct, heating-cooling heat transfer devices, a blower, a return air inlet and air outlets, and is characterized in that the inner side of a ceiling is provided with an interlayer; an inner wall surface and inner wall corners are respectively provided with vertical air ducts; the ceiling interlayer is communicated respectively with the vertical air ducts so as to form the circulation air duct; the return air inlet in the center of the ceiling interlayer is provided with a return air inlet blower; the air outlets around the return air inlet blower are provided with the heating-cooling heat transfer devices; and a plurality of air outlets and air outlet blowers are arranged on the surfaces of the vertical air ducts. The phytotron has the advantages that the phytotron can improve the precision and evenness of indoor temperature control, prevent culture mediums and seedlings from being blow-dried by strong wind, and ensure tidy regular indoor and outdoor geometric shapes.

The invention belongs to molecular seed breeding and relates to a molecular seed breeding method for breeding a new paddy rice variety which has heat resistance during heading and flowering stage and filling stage. The method includes the steps of: 1) with a mid-indica two-line elite restorer line 9311, which is assigned the accession number of CGMCC No.12532, as a female parent, and an F1 generation, which is obtained by hybridizing Yuanjiang common wild rice single-fragment substitution lines YJ07-04-06 and YJ01-05-03 assigned the accession numbers of CGMCC No.12534 and CGMCC No.12535, as a male parent, performing intercross for 1 generation, performing backcross for 3 generations with a recurrent parent, and then performing selfing for 3 generations to obtain a stable homozygous plant strain BC3F4, wherein each generation is harvested in a single spike manner; and 2) identifying and selecting a new paddy rice variety by means of molecule marker assisted selection with combination of phytotron for simulating high temperature stress. By means of the method, a new paddy rice variety "Yuanye No.3" having heat resistance during the heading and flowering stage and filling stage has been cultured. Target characters are selected through molecule marker assistance, so that a restorer line having heat resistance during the heading and flowering stage and filling stage can be cultured within 3-4 years quickly and high-effectively.

The invention provides a young shoot grafting method for virus removal of apples. The method comprises the following steps: disinfecting grafting tools in advance; detecting virus carrying conditionsof leaves located at 4-5cm the top of a new shoot which is grown to 15-20cm and treated by virus removal, retaining 1-2 young leaves on the top end of the new shoot of which target virus is removed, and cutting a shoot tip with the length of 3-4cm to be used as a scion; selecting a potted virus-free annual growth rootstock seedling with the main stem diameter of 1.5cm or more to be used as a virus-free rootstock, performing rootstock cutting at the height of 25-35cm far away from the ground; and after grafting is performed, performing moisturizing by performing bagging on the shoot tip and a connection opening part, performing moisturizing culture in a phytotron for 10-15 days, and performing seedling exercising to obtain a virus-free seedling of apples. According to the method provided bythe invention, shoot tips obtained by special ways such as an insufficient maturity degree, seasonal limitation, tissue culture, and anti-seasonal seed introduction can perform apple seedling grafting, preservation and reproduction at any time, the problem of a low survival rate of young shoot grafting in a conventional grafting method is solved, and a novel method is opened up for reproduction of virus-free seedlings.

The invention discloses a remediation method for soil contaminated by heavy metal. Paddy rice is planted in soil contaminated by both cadmium and copper, a carbon dioxide fertilizer is applied to enable the carbon dioxide concentration of local atmosphere to rise, the condition for increasing the carbon dioxide concentration is open-top phytotron, carbon dioxide rises to 860+/-50 [mu] l l<-1>, the paddy rice is Rongyou 398, Shanyou 428, Yueza 889, Tianyou 390, Guinongzhan or Yinjingruanzhan. The remediation method has the remarkable advantage that the plant remediation efficiency is high.

The invention discloses an outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method. The method includes the steps that natural inoculation and feeding are conducted on tested insects; supplied test fungicides are prepared into liquid of a set concentration; branches with insects of potted nursery stock are integrally soaked into the prepared liquid, and then the soaked branches with insects are enveloped by a thin and soft gauze element; the potted nursery stock which is soaked with pesticide is placed in a phytotron with the light dark period being L:D=14:10 h, the temperature being 25+/-1 DEG C and the relative humidity being 75% to be fed; the death and survival condition of the tested icerya purchasi is examined after the icerya purchasi is fed for 24 hours, and the death number and the survival number are recorded; a living body plant is used for cultivating the tested insects which are soaked with the liquid, the food freshness of the icerya purchasi is guaranteed, sufficient nutrients are provided for the icerya purchasi, testing is conducted under a natural environment growth condition where the icerya purchasi is placed, the accuracy of biological assay results is effectively improved, hence, the icerya purchasi population resistance level can be known comprehensively, accurately and precisely, and a basis is provided for reasonably utilizing pesticide and making a resistance treatment plan accurately.

The invention provides a determination method for the CO2 feeding amount of greenhouse culture green vegetable plants. The method comprises the steps of 1, photosynthetic tester selection and adjusting; 2, preparation of a small phytotron; 3, culture and preparation of to-be-tested plants; 4, manufacturing of a CO2 constant chamber; 5, measurement setting; 6, determination of net photosynthetic rates under different temperatures; 7, determination of net photosynthetic rates under different illumination intensities; 8, determination of net photosynthetic rates under different temperature and illumination intensity combinations; 9, fitting of equations; 10, application. Due to the data collection, computer technology application and other modes, the artificial feeding CO2 time and consumption are determined, CO2 consumed by plant use in a greenhouse can be supplemented in time in production, and the scientific basis is provided for increasing the greenhouse culture green vegetable yield;the method has the advantages of being low in cost, easy and convenient to operate and remarkable in effect.

The invention relates to the technical field of power transmission and distribution, and discloses an icing shape test-based overhead power transmission line galloping prediction method and system, which gets rid of constraints of a field detection device and realizes scientific and reliable line galloping prediction. The method comprises the steps of carrying out icing shape tests of different line structures under different meteorological conditions in a phytotron to obtain observation records; according to the observation records of the phytotron, drawing an icing thickness increment curve, and through curve fitting, establishing a function relationship of icing thickness increment degree; determining an icing non-uniformity calculation formula, drawing an icing non-uniformity curve according to the observation records of the phytotron, and through curve fitting, establishing a function relationship of icing shape non-uniformity; and according to the fit icing thickness increment degree function, predicting icing thickness in a natural condition, according to the fit icing shape non-uniformity function, predicting icing non-uniformity in the natural condition, and based on a galloping prediction model, performing power transmission line galloping prediction.

The invention provides a breeding method of an excellent novel germplasm of soybeans. In the method, recurrent selection is carried out by using a male genic sterile gene, the defects of artificial castration hybridization are completely overcome, and the randomness of cross parent selection and crossbreeding efficiency are greatly improved. The recurrent selection technology of the male genic sterile gene is introduced into group hybridization, intercross, backcross and composite cross plans of soybean germplasm, through hybridized combination prepared in a summer net chamber, off-season multiplication for generation addition in winter and secondary generation addition in an artificial phytotron in spring, three generations are realized each year, marker-assisted selection is used, largebatches and multiple types of novel germplasm materials of soybeans can be rapidly obtained by non-destructive quality detection, indoor resistance identification and multi-point adaptability identification, precious and excellent parents are provided for breeding of new varieties, and soybean breeding has infinite potentials.

The invention belongs to the field of molecular seed breeding and relates to a molecular seed breeding method for breeding a new paddy rice variety which has heat resistance during heading and flowering stage. The method includes the steps of: 1) with a mid-indica two-line elite restorer line 9311, which is assigned the accession number of CGMCC No.12532, as a female parent, and a wild rice chromosome fragment introgression line YJ07-04-06, which is assigned the accession number of CGMCC No.12534, as a male parent, performing hybridization for 1 generation and backcross for 3 generations, and then performing selfing for 3 generations to obtain BC3F4, wherein each generation is harvested in a single plant manner; 2) identifying and selecting a new paddy rice variety by means of molecule marker assisted selection with combination of phytotron for simulating high temperature stress. By means of the method, a new paddy rice variety "Yuanye No.2" having heat resistance during the heading and flowering stage has been cultured. Target characters are selected through molecule marker assistance, so that a restorer line having heat resistance during the heading and flowering stage can be cultured within 3-4 years quickly and high-effectively.

The invention discloses a method for transforming in-vitro soybean growing points with a gene gun, belonging to the technical field of bioengineering. The method is characterized by making bullets with carriers containing target genes, loading the bullets into the gene gun, then bombarding aseptic seedlings and performing inoculation induction to obtain T0-generation plants; performing rooting induction and seedling hardening by opening a vessel lid on the T0-generation plants, and then inducing the plants to bloom and fruit to obtain T1-generation seeds; and finally transplanting the T1-generation seeds to a phytotron or large fields to obtain T2-generation plants and homozygous positive transformant thereof. The method has the following advantage: by utilizing the characteristic that the plants prepared from the in-vitro soybean growing points are relatively easy to regenerate, the transformation efficiency can be obviously improved.

The invention relates to the technical field of light regulation and control, in particular to a molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling. According to the method, during an experiment, excellent pakchoi seeds are firstly obtained, cultivation experiment work is carried out, finally obtained experiment data are more accurate, the credibility of the experiment is improved, then the seeds are placed in sterile water to be soaked, the seeds can rapidly absorb water, the germination time of the seeds is shortened, meanwhile, sterile water is used for soaking the seeds, the influence of bacteria on the seeds can be reduced when the seeds absorb water, the survival rate of the seeds is increased, meanwhile, the temperature and humidity of a seedling chamber and a phytotron are manually adjusted, the temperature and humidity are manually adjusted to be suitable for the growth of the pakchoi, the growth speed of the pakchoi is increased, during growth, UV-A coupling and blue light with different light intensities are used for light regulation and control work, and the anthocyanin component rich in the pakchoi is increased along with the change of time.