71 results about "Resistant phenotype" patented technology

The invention is based on the reaction of Duplex Mutational Vector in a cell-free system containing a cytoplasmic cell extract and a test plasmid. The reaction specifically converts a mutant kanr gene to recover the resistant phenotype in transformed MutS, RecA deficient bacteria. Using this system a type of Duplex Mutational Vector termed a Non-Chimeric Mutational Vector, having no RNA:DNA hybrid-duplex is shown to be an effective substrate for eukaryotic enzymes. The invention concerns the use of Non-Chimeric Mutational Vectors protected from 3' exonuclease attack in eukaryotic cells. Such protection can be conferred by replacement of a tetrathymidine linker by a nuclease resistant oligonucleotide, such as tetra-2'-O-methyl-uridine, to link the two strands of the recombinagenic oligonucleobase.

The present invention provides for a method of simultaneously treating both cancer and the Multidrug Resistance Phenotype via inhibition of cellular thiols, such as Glutathione S-Transferase (GST). This enzyme is overproduced in leukemia and solid tumor cells and is one of the main pathways involved in the Multidrug Resistance phenotype. The treatment provides for the administration of a chemically inert pro-drug, designed to be a specific substrate for the GST enzyme that, once cleaved, liberates the bioactive toxin Nitric Oxide (NO) intracellularly at the site of a malignant growth. NO then acts to inhibit the growth of the malignant cells and to induce cellular differentiation and apoptosis therein, effectively treating an existing cancerous condition. Additionally, once NO is liberated from the pro-drug, the remaining structure acts to inhibit further GST activity, providing a treatment for the Multidrug Resistant phenotype.

The present invention stems from the realization that the cell surface content of sialic acids is associated with drug susceptibility and resistance in neoplastic and damaged cells. A general decrease in the amounts of the α2-6 linked sialic acid has been confirmed in resistant phenotype compare to its corresponding sensitive parental cells. Treating the resistant phenotype with neuraminidase, an enzyme that removes the sialic acid from the sugar chain, facilitates the drug internalization and reinstalls the cell susceptibility to the drug. Based on these observations, methods for predicting the resistance to a number of drugs and detecting multi-drug resistanc in neoplastic and damaged cells have been invented.

The invention relates to a method for screening important characteristic genes related to the drug resistance phenotype of bacterial based on a machine learning technology. According to the method, for the antibiotic resistance of the bacterial, on the basis of the BGWAS thought, target bacterial genomes on a public platform or large-sample-size bacterial strain genome data obtained after current collection, sequencing and assembly, and corresponding antibiotic drug susceptibility test results are collected, and correlation analysis between genotypes and drug resistance phenotypes is carried out by using the machine learning method, so that important characteristic genes (non-core drug-resistant genes) related to the drug resistance phenotypes are screened out, weight coefficients of the important characteristic genes are obtained;and finally the reliability of the drug-resistant genes related to drugs is determined by using ROC analysis.

The invention discloses a molecular marker of a main active gene BLS1 of paddy rice resisting bacterial streak and an application of the molecular marker. A bacterial streak resistant backcross inbred line obtained from deriving an ordinary wild paddy rice resistant source DP3 is crossed with a bacterial streak-infected nonglutinous rice variety 93-11 to obtain an offspring F1 hybrid and then the hybrid is self-fertilized to obtain F2 generation, genetic linkage analysis is performed for a gene type and corresponding resistant phenotype of each family of F2, and the bacterial streak-resistant main active gene BLS1 sourced from the ordinary wild paddy rice DP3 is detected. Molecular markers capable of being used for breeding such as RM19382, RM19391, RM19400, RM19402 and RM510 are obtained. The molecular markers can effectively detect whether the bacterial streak-resistant ordinary wild paddy rice DP3 and erivative varieties (lines) thereof contain a main active gene site, thereby greatly improving the selection efficiency of the bacterial streak resistant paddy rice, and obtaining the bacterial streak resistant paddy rice variety containing the BLS1 gene.

The invention discloses a cell model of HIV strain phenotypic drug resistance analysis and special pseudotype slow virus thereof; the invention constructs plasmid for expressing Gag protein of HIV, recombinant slow-virus plasmid for expressing reporter gene and helper plasmid for expressing Rev protein of HIV; under the effect of the helper plasmid pVSV-G, the three plasmid and HIV reverse transcriptase and the gene fragment of protease which are amplified from strain can obtain the pseudotype slow virus of the HIV reverse transcriptase and protease gene containing strain. The pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the strain phenotypic resistance cell model based on the reporter gene. The cell model of the invention can carry out phenotypic resistance analysis to the strain, the reporter gene leads the cell model to have super high sensitivity, and the cell model of HIV strain phenotypic drug resistance analysis is applicable to the phenotypic drug resistance analysis of HIV strain in Chinese population.

The invention provides a genome selection method for breeding Plectropomus leopardus disease-resistant improved variety. The method comprises the following steps of: constructing a Plectropomus leopardus disease-resistant reference group by using Plectropomus leopardus young fishes from different geographical sources, and taking individuals which have disease-resistant phenotypes but are not included in the reference group as a verification group, wherein the Plectropomus leopardus young fishes used for constructing the disease-resistant reference group is obtained after disease resistance screening; and predicting a disease-resistant character genome estimation breeding value of the verification group by using the selected SNP sites and the constructed Plectropomus leopardus disease-resistant reference group, and selecting alternative parents by using the disease-resistant character genome estimation breeding value to obtain individuals with strong disease resistance for disease-resistant improved variety cultivation. According to the invention, the method for breeding the Plectropomus leopardus disease-resistant improved variety based on the whole-genome selection technology can be used for screening parents with high disease resistance, the selected parents can be directly used for breeding Plectropomus leopardus, and an efficient technical means is provided for improving the survival rate of Plectropomus leopardus breeding group.

The invention discloses an identification method for a sulfur-resistant hybrid breeding progeny of saccharomyces bayanus, belonging to an identification method for a novel saccharomyces bayanus breeding strain. The identification method comprises the following steps: saccharomyces bayanus hybridization; sulfur resistance measurement of a saccharomyces bayanus strain; saccharomyces bayanus DNA (Deoxyribonucleic Acid) extraction and purification, wherein a PCR (Polymerase Chain Reaction) system taking (i) FZF1 (/i) as a primer and a procedure are adopted; PCR primer sequencing and identifying; and further ISSR (Inter-Simple Sequence Repeat) analysis for the sulfur-resistant hybrid breeding progeny. In the embodiment, 18 suspected hybrid progenies are obtained from 3 hybridized combinations. The PCR analysis results are as follows: the 18 suspected hybrid progenies contain 5 strains only containing sulfur-nonresistant parental characteristic bands, 2 strains containing sulfur-resistant parental characteristic bands and other 11 strains containing biparental characteristic bands. The ISSR analysis for 8 sulfur-resistant hybrid progenies proves that the same strain has two single colonies with the completely-consistent fingerprint spectrums. According to the invention, 6 sulfur-resistant hybrid breeding progeny strains of saccharomyces bayanus are obtained according to a DNA sequence and an ISSR atlas and through sulfur-resistant phenotype screening, so that the accuracy rate for identifying the hybrid breeding progeny strains is remarkably increased.

The invention discloses a cold-resistant gene engineering application method of rice OsICE2 gene. The method includes: (1) gene cloning, (2) vector construction, (3) transgenosis, (4) analysis of the cold-resistant phenotype of OsICE2 by a transgenic technology, and (5) comparative analysis of a transgenic plant and a control plant in the cold-resistant physiological aspect. By the steps, the method determines the influence of OsICE2 gene on protein expression quantity under low temperature stress, and finds that a transgenic rice strain can slow down the reduction of protein expression quantity of RuBi sCO subunit binding-protein alpha subunit, RuBi sCO activase small isoform precursor, Chlorophyll a-b binding protein and isocitrate dehydrogenase under low temperature stress, and improves the expression increase range of 70 kDa heat shock-related protein.

The present invention relates to new uses of daphnetin in inhibition of Helicobacter pylori. According to the present invention, the daphnetin can well kill a variety of drug-resistant phenotype Helicobacter pylori; particularly the results show that the daphnetin can produce the zone-of-inhibition with a diameter of 39-53 mm at a concentration of 100 [mu]g/disc, and has the minimum inhibitory concentration (MIC) of 25-100 [mu]g/ml; and the new use of the daphnetin provide the new option for Helicobacter pylori inhibition drugs.

The invention discloses an application of an oidium heveae-resistant gene ROH1 to arabidopsis. The invention provides an application of ROH1 protein, a coding gene of the ROH1 protein or a recombinant vector containing the coding gene to the regulation and control of plant disease resistance; an amino acid sequence of the ROH1 protein is a sequence 2 in a sequence table. An arabidopsis mutant with ROH1 mutation is complemented with the gene ROH1, so that a transgenic plant is obtained and an oidium heveae-resistant phenotype of wild-type arabidopsis is recovered. Therefore, the fact proves that ROH1 is the oidium heveae-resistant gene and lays a very good foundation for breeding for disease resistance.

The invention relate to a cDNA sequence of glycerol-3-phosphate dehydrogenase genes DvGPH1 and DvGPDH2 associated with glycerol synthesis, and an amino acid sequence of an encoding protein. The expression vector of the DvGPH1 or DvGPDH2 gene is translated into a yeast gpd1delta cell. The yeast cell shows a salt resistant phenotype on a culture medium containing salts, thereby verifying that the two genes can strengthen glycerol synthesis in the yeast cell and improve the salt resistance of the yeast cell, and simultaneously verifying that the two genes have good application value on improving the glycerol synthesis amount in organisms such as the yeast through genetic engineering means and strengthening the salt resistance of the organisms. As the invention further improves the glycerol-3-phosphate dehydrogenase activity and the capability of catalyzing and synthesizing the glycerol of the two gene products and the salt resistance of a corresponding yeast transformant through the deletion of part of sequences of the DvGPH1 or DvGPDH2 genes, the two deletion type gene derivatives have better prospect in the application fields. In addition, the invention verifies that the expression of the DvGPH1 and DvGPDH2 genes in a dunaliella is subjected to the induction of salt stress, thereby showing that the two genes are the key enzymes for dunaliella synthetic glycerol and further providing the theoretical base for the application of the two genes to the fields.

The invention discloses a multivalent transgenic insect-resistant rice genotype identification primer pair and method. A series of multiplex primers are designed aiming at transgenic rice TT51-1 (trans-cry1Ab/cry1Ac fusion gene), T2A-1 (trans-cry2A gene) and T1C-19 (trans-cry1C gene) and used for detecting genotypes of univalent, divalent and trivalent trans-Bt genes in rice breeding material so as to confirm Bt gene homozygous or heterozygous states as soon as possible, and the problem of failure in distinguishing of univalence and multivalence in insect-resistant phenotype identification issolved. According to experiments, a detection method is high in specificity, expensive or high-time-consumption detection means are avoided, and an insect-resistant transgenic rice breeding process can be accelerated.

The invention discloses a single-cell Raman clinical drug resistance rapid detection kit and a detection method. The single-cell Raman clinical drug resistance rapid detection kit comprises an incubation solution, a stationary solution I and a stationary solution II, wherein the incubation solution contains heavy water; the stationary solution I contains normal saline or PBS; and the stationary solution II contains absolute ethyl alcohol. The kit is used for stable isotope labeled single-cell Raman drug resistance detection, can realize rapid detection of drug resistance phenotypes of trace microorganisms in a clinical sample without dependence on proliferation, and can solve the problem of slow detection of clinical drug-resistant pathogenic bacteria in the prior art.

The present invention provides several modified ABC transporter polypeptides that exhibit novel localization in the plasma membrane of polarized and non-polarized cells. The modified ABC transporter of the invention comprises the amino acid sequence of a native apically targeted ABC transporter, in particular cMOAT, MDR3 or MRP4, wherein the terminal tripeptide T-K-F motif of said native ABC transporter is mutated. The isolated modified ABC transporter polypeptide of the invention, and the nucleotide sequence encoding said polypeptide, have utility in the following applications: First, they are used to induce a drug resistant phenotype in a cell. Second, they are used to protect non-polarized cells during chemotherapy and other therapeutic applications. Third, they are used to produce novel cell lines that are used to screen for novel agonists or antagonists of the corresponding native ABC transporter polypeptides.

This disclosure provides a system for obtaining genetically altered primate pluripotent stem (pPS) cells. The role of the feeder cells is replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. The cells can be genetically altered with a viral vector or DNA/lipid complex, and then selected for successful transfection by drug-resistant phenotype in the transfected cells. The system allows for bulk proliferation of genetically altered pPS cells as important products for use in human therapy or drug screening.

This disclosure concerns methods and compositions for identifying canola plants that have a blackleg resistant phenotype. Some embodiments concern molecular markers to identify, select, and/or construct blackleg resistant plants and germplasm, or to identify and counter-select plants that are susceptible or have low resistance to blackleg disease. Some embodiments concern molecular markers to identify, select, and/or construct blackleg resistant plants that carry the rlm2 gene. This disclosure also concerns canola plants comprising a blackleg resistant phenotype that are generated by methods utilizing at least one marker described herein.

The present invention relates to a method for detecting resistant microorganisms to a therapeutic agent in a biological sample, comprising the following steps:

• • a. inoculate the said sample, uncultured, on a first tube with, and on a second tube without, at least one therapeutic agent; preferentially further including at least one lysing agent and/or a buffer, and/or a suitable culture medium, or put the sample on a separation serum tube; and incubated it;
  • b. add to both tubes a fluorescent marker;
  • c. perform a fluorescence analysis for obtaining one or more fluorescence or growth parameters for each of the two tubes;

wherein the microorganisms resistant phenotype of the biological sample to said therapeutic agent is obtained by comparing the one or more fluorescence parameters between the two tubes.

Therefore, the present invention is useful in laboratory procedures or routines for the detection of the susceptibility of different microorganisms to a therapeutic agent, to the determination of microorganisms resistance mechanisms or even to evaluate the amount of antimicrobial drug in the biological sample.