The invention discloses an
oligonucleotide chip labeling a random primer PCR amplification based on a
biotin-
avidin-cy3
reaction system, which is made by a substrate, and CMV, HSVI, RV, EBV or TB
pathogen probes, a
negative control, a
positive control and a blank control that are coated on the substrate in an array way, wherein the substrate is a surface
aldehyde glass slide; the
pathogen probes are 10 of CMV-1, CMV-2, HSVI-1, HSVI-2, RV1, RV2, EBV1, EBV2, TB1 and TB2 probes with 70mer; the
positive control is a synthesized marker probe; and the blank control is double
distilled water. The invention performs the
biotin-11-dUTP amplified labeling to various
pathogen target sequences by using the random primer and detects the pathogen by adopting a method of increasing the labeling efficiency through using a
biotin-
avidin-cy3
signal amplification
system, has high detection sensitivity and obviously shortens the detection cost and the detection time. The technical
system can be suitable to the fields of clinical detection, health supervision, customs
quarantine control, scientific research, and the like.