Method for detecting transgenic tomato by using in-situ synthetic microfluidic chip

A microfluidic chip and in-situ synthesis technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. It can solve the problems of not meeting the needs of food supervision, low efficiency, narrow detection range, etc.
CN103290128AInactive Publication Date: 2013-09-11ZHEJIANG SCI-TECH UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG SCI-TECH UNIV
Publication Date
2013-09-11
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a method for detecting a transgenic tomato by using an in-situ synthetic microfluidic chip. The method comprises the following steps of: 1) designing a chip probe for detecting an exogenous gene of the transgenic tomato; 2) preparing an oligonucleotide chip; 3) extracting genome deoxyribonucleic acid (DNA) in a tomato sample to be detected; 4) marking a specific segment of the genome or the entire genome by a fluorescent agent; 5) chip hybridization; 6) judging whether the sample belongs to the transgenic tomato variety. By adopting the method, an oligonucleotide probe is designed according to the sequence information of the known tomato exogenous gene which has been publically reported; the microfluidic gene chip is prepared by utilizing an in-situ synthesis method, so as to achieve simultaneous detection of existence of the exogenous genes related to insect resistance, antiviral action, herbicide resistance, delayed fruit, softening resistance, storage resistance and the like on the same chip. Thus, an effective method is provided for rapid detection and identification of the transgenic ingredients of the tomato and highly processed products; a foundation is provided for building and perfecting a transgenosis detection system and a supervisory system.
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Description

technical field

[0001] The invention relates to a method for detecting and identifying transgenic components in tomato, in particular to a method for detecting known exogenous genes in tomato by using an in-situ synthetic microfluidic chip. Background technique

[0002] Tomato (Solanum lycopersicum) is one of the important crops of Solanaceae. Its fruit is rich in vitamins, carotene, and lycopene, and has high nutritional value and health benefits. It is an important fruit and vegetable product in the world. In the past ten years, the planting area and output of tomato in the world have increased by 38% and 42% respectively, and the industrialization trend is obvious. Since 1994, when the extended ripening and fresh-keeping transgenic tomato FLAVR SAVR bred by Calgene Corporation of the United States was approved to enter the market, various improved tomato varieties have been reported one after another, and some of them have been approved. It has become an irresistible tre...

Claims

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