The invention discloses a biological control agent for fusarium wilt and a preparation and use method of the biological control agent. The biological control agent comprises the following components of not less than 1*10<7>cfu/mL of cellulose-producing trichoderma viride, not less than 0.5*10<7>cfu/mL of aspergillus niger, 0.8*10<9>cfu/mL of bacillus subtilis and 0.8*10<9>cfu/mL of bacillus mucilaginosus. The prepared biological control agent has an antagonism on antagonism, is capable of increasing disease prevention capability, and has functions of activating soil nutrients, promoting growth of root systems of crops, and inducing the improvement of disease resistance when being applied, thereby improving control effect of fusarium wilt of the crops, and enabling the incidence rate to be lower than 10 percent. The biological control agent can completely replace a chemical agent, has no pollution, residue and biological toxicity during use, ensures that the yield of crops is increased by above 15 percent, and is suitable for controlling fusarium wilt of cucurbit plants such as cucumber, watermelon, muskmelon, bottle gourd and towel gourd, and solanaceae crops such as tomato and eggplant.