2 results about "Lauraceae" patented technology

This invention discloses a method for increasing the lignin content in the roots of Lauraceae fruit trees, belonging to the field of plant physiological regulation technology. To increase the lignin content in the roots of Lauraceae fruit trees such as avocado trees, this invention employs the following techniques: application of a lignin-synthetic nutrient-enhancing solution; directional pulsed electric field treatment to enhance the permeability of the fruit tree's epidermal cell membranes; activation of key enzymes in phenylpropanoid metabolism by irradiation with specific wavelengths of ultraviolet light; and synergistic application of mechanical stress technology to stimulate and induce vascular tissue differentiation. By treating the roots of Lauraceae fruit trees, not only can the lignin content in the root system be increased by more than 30%, but the root system's stress resistance and mechanical strength can also be improved by more than 45%, while maintaining normal physiological metabolism of the plant. This provides a new solution for lodging-resistant cultivation and root rot prevention in Lauraceae fruit trees.

The invention discloses an agrobacterium rhizogenes mediated transformation method for transgenic hairy roots of complete leaves of cinnamon. The stable and repeatable agrobacterium rhizogenes-mediated hairy root transformation system is established for the first time aiming at the cinnamomum cassia which is a cinnamomum camphora woody plant with difficulty in genetic transformation, and the technical difficulty in gene function research is solved. It is found that in cinnamon, only on the premise that the overall integrity of leaves is kept, the stable agrobacterium rhizogenes infection and hairy root induction can be achieved by slightly scratching the leaves perpendicular to leaf veins in cooperation with an agrobacterium infection solution with a certain concentration and vacuumizing treatment, and the hairy root induction effect is achieved. And a stable material is provided for subsequent hairy root induction of a complete transgenic regeneration plant. The agrobacterium rhizogenes-mediated hairy root transformation method provided by the invention can efficiently induce plants to generate transgenic roots, lays a foundation for mining good cinnamon genes in the future, and provides a convenient and rapid way for researching biological functions of related genes in the roots.