35 results about "Mangrove plants" patented technology

The invention discloses a seedling raising and transplanting method for mangrove plant Aegiceras corniculatum based on external application of gibberellin, and belongs to the technical field of saline-alkali plant cultivation and mud flat ecological restoration. The method mainly comprises the four stages of seed disinfection and gibberellin soaking, germination cultivation, seedling cultivation, salinity domestication and mud flat transplanting. After being disinfected, the seeds are soaked in a gibberellin solution with the concentration being 300 mg / L, gibberellin with the same concentration is sprayed regularly during germination, and the germination rate and the germination speed are increased. In the seedling stage, humus soil and mud flat bottom mud mixed matrix is adopted for cultivation, and growth is promoted by spraying 100 mg / L gibberellin to leaf surfaces and injecting 25 mg / L gibberellin to rhizosphere. After seedlings are formed, the seedlings are subjected to step-by-step salinity domestication (5 per thousand to 30 per thousand), gibberellin spraying and rhizosphere injection are carried out at regular intervals, and the stress resistance of the seedlings is enhanced. The method can effectively shorten the seedling raising period of Aegiceras corniculatum, improve the seedling raising efficiency, enhance the adaptive capacity of seedlings to the tidal zone environment, and facilitate rapid recovery of a mangrove forest ecosystem.

This invention discloses a method for hardwood cutting propagation of the mangrove plant *Avicennia marina*, comprising: S1: Selection and preparation of cuttings: In early July, select vigorous, healthy, and disease-free superior single plants, collect robust perennial branches, keep them moist after collection, bring them back to the cutting site, and cut them into cuttings on the same day; S2: Pretreatment of cuttings: Disinfect the cuttings obtained in step (1) by soaking them in a 700-fold carbendazim solution for 10 minutes, and then let them air dry naturally; subsequently, quickly dip the cuttings in a 1000 mg / L NAA and IBA hormone solution for 30 seconds, and insert them into the substrate of the seedbed to a depth of 3-4 cm. This method for hardwood cutting propagation of the mangrove plant *Avicennia marina* provides technical support for the industrialized and large-scale production of superior *Avicennia marina* seedlings, and can also ensure the demand for superior seedlings for the ecological restoration of coastal mangroves.

The invention discloses a seed preservation method of cryptophytic mangrove plant avicennia marina. The method comprises the following steps: (1) selecting healthy and full avicennia marina seeds without plant diseases and insect pests, removing outer skin, soaking and disinfecting in a carbendazim aqueous solution for 15 minutes, washing with clear water, and airing; (2) soaking the avicennia marina seeds treated in the step (1) in a chitosan solution for 1 min, taking out, and airing; and (3) embedding the avicennia marina seeds treated in the step (2) into a preservation matrix by taking wet sand as the preservation matrix, then putting the seeds and the preservation matrix into a plastic package bag for sealing, so that the seeds are still kept in a buried state in the bag, and finally storing in a dark environment at 14 DEG C. The germination rate of the avicennia marina seeds stored by the method can still reach 40% after 4 months, the storage time of the avicennia marina seeds is remarkably prolonged, and convenience is provided for seedling raising and ecological restoration of the avicennia marina.

The invention relates to the technical field of internal circulation low-carbon systems, in particular to a mangrove plant acanthus ilicifolius and bostrichthys sinensis pond planting and breeding coupled internal circulation low-carbon system which comprises three biological components, namely mangrove plant acanthus ilicifolius, bostrichthys sinensis and seawater tilapia, and the three biological components coexist in a low-salt seawater environment with the salinity of 5-8 to form a substance circulation chain; according to the method, the tilapia mossambica culture pond serves as a hub, residual feed and excrement of the tilapia mossambica culture pond are conveyed to the acanthus ilicifolius planting area along with circulating water to provide fertility, chippings of mangrove plant acanthus ilicifolius overflow back to the tilapia mossambica culture pond, and therefore the medicinal and edible value of tilapia mossambica is increased, 70-80% of tilapia mossambica meat mixed feed is fed to Nitrogen and phosphorus-rich bottom mud in a tilapia pond cleaned in winter is recycled as acanthus ilicifolius organic fertilizer, so that resource circulation of'feeding trees with fishes and fattening the fishes with the trees' is realized, and input of exogenous pollution is reduced.

The invention provides a floatable mangrove plant support which comprises at least one standard unit, each standard unit comprises a first bamboo raft, a second bamboo raft, a connecting assembly and a bearing assembly, and the first bamboo raft is provided with a plurality of first penetrating holes at intervals; the second bamboo rafts are connected with the first bamboo rafts through fixing bamboo tubes and provided with a plurality of second penetrating holes corresponding to the first penetrating holes at intervals. The connecting assembly comprises a plurality of connecting rod pieces arranged at intervals, each connecting rod piece comprises a tension spring pipe, a tension spring and a transmission pull rod, the tension spring pipes are hinged to the second bamboo rafts, the transmission pull rods are connected with the tension spring pipes through the tension springs, and limiting stop levers are arranged on the transmission pull rods; the bearing assembly comprises a plurality of bearing rod pieces arranged at intervals, the two opposite ends of each bearing rod piece slidably penetrate through the corresponding first penetrating hole and the corresponding second penetrating hole respectively, the bearing rod pieces can be stressed to rotate, a plurality of clamping grooves are formed in the bearing rod pieces in the length direction at intervals, and the clamping grooves can be clamped with the limiting stop rods. The problem that mangrove plants are difficult to transplant in an intertidal zone can be solved.

The application discloses a kind of difficult stand beach intertidal alien mangrove plant Laganum community transformation method, belong to mangrove planting technical field;Steps include: S1, mangrove present situation investigation;S2, beach protection repair measure;S3, Laganum pruning or felling;S4, plant mangrove forest;S5, management and protection;S6, effect evaluation;The method can be grown to Laganum community renewal reconstruction in seawater scouring area, avoid reconstruction failure risk, compared with other conventional methods reconstruction repair early canopy forestation;After taking beach protection measure, the beach of repair area is stable, avoid being lost by tidal scouring and leading to sediment;Use old fishing net to protect, avoid that marine litter enters repair area;Use small block to level, keep original Laganum root system, there are few biologicals in woodland in early reconstruction period, such as crab, reduce the interference and damage to new planting seedling, guarantee mangrove seedling survival rate;Using seedling planting that is cultivated under the condition of high salinity environment, seedling resistance is strong, recovery growth speed is fast, survival rate is high.

The invention discloses an endogenous promoter AMGT1P11 of mangrove plant avicennia marina and application of the endogenous promoter AMGT1P11, and belongs to the technical field of gene engineering, and the nucleotide sequence of the endogenous promoter AMGT1P11 comprises a sequence as shown in SEQ ID NO: 1, a sequence as shown in SEQ ID NO: 2, a sequence as shown in SEQ ID NO: 3 and a sequence as shown in SEQ ID NO: 4; or a sequence which has at least 70% homology with the sequence as shown in SEQ ID NO: 1 and has the same function as the sequence as shown in SEQ ID NO: 1; or, one or more basic groups are added, substituted, inserted or deleted in the sequence shown in SEQ ID NO: 1, and the sequence has the same function as the sequence shown in SEQ ID NO: 1. The AMGT1P11 contains a reported salt-induced cis-acting element GT1-motif (GGTTAA, a new reference can be provided for research of a mangrove plant salt-tolerant mechanism, meanwhile, the promoter can regulate and control expression of exogenous target genes in dicotyledons and monocotyledons, a brand new tool and selection are provided for plant transgenic engineering, and an important foundation is laid for plant genetic improvement.

The present application relates to the field of remote sensing monitoring, in particular to a mangrove inter-specific classification method and device based on unmanned aerial vehicle remote sensing, adopting a decision tree learning algorithm to update feature threshold values in a feature corresponding relationship of a plurality of mangrove plant categories in an initial multi-modal plant knowledge graph, obtaining an updated multi-modal plant knowledge graph, performing inter-specific classification reasoning according to a plurality of types of feature data in the extracted feature data set of the region to be classified, feature values corresponding to the feature data, and the updated multi-modal plant knowledge graph, obtaining a mangrove inter-specific classification result, which integrates classification knowledge, overcomes the shortcomings of a method that simply relies on data, and improves the accuracy, efficiency and reliability of mangrove inter-specific classification.

The invention discloses a culture pond mangrove forest restoration appropriate elevation determination system based on tide level numerical simulation, and relates to the technical field of marine ecological restoration, and the system specifically comprises an acquisition processing module, a tide level model module, a scene simulation module, a relation analysis module, a screening comparison module and an optimization decision module. The system firstly collects basic data; establishing a three-dimensional hydrodynamic numerical model based on a hydrodynamic principle; simulating water level responses under different working conditions according to the basic data and the gate operation rule; calculating the daily average flooding time of any given elevation point in the culture pond in the simulation period through statistical analysis, and establishing a corresponding relation curve between the elevations of different positions and the daily average flooding time; comparing the flood-tolerant duration data of the target mangrove plant species with the corresponding relation curve as a screening condition; and finally, calculating a theoretical appropriate elevation range, and taking the appropriate elevation range determined under the working condition as a basis for guiding the appropriate elevation design for repairing the mangrove forest in the culture pond.

The invention discloses an indoor seedling raising method for mangrove plant red sea olive. The method comprises three stages of seed pretreatment, seed germination and seedling cultivation. The light environment, the temperature, the humidity, the nutrition supply and the salinity of the substrate are accurately regulated and controlled under the condition of full manual control by adopting the culture substrate with a specific proportion, and the dependence of traditional seedling culture on natural conditions is effectively overcome. According to the method, standardization and controllability of red sea olive seedling raising are achieved, the growth speed of red sea olive seedlings is remarkably increased, and the method is suitable for large-scale and annual seedling raising production and has important application value for mangrove forest wetland restoration and ecological engineering.

The invention discloses a salt-tolerant flooding-resistant mangrove plant variety breeding method, belongs to the technical field of mangrove plant breeding, and systematically carries out salt-tolerant flooding-resistant excellent mangrove variety breeding work through three links of germplasm resource collection, character evaluation and variety breeding. The method comprises the following steps: firstly, carrying out resource census on mangrove plants in a natural distribution area, comprehensively evaluating the growth and resistance performance of the mangrove plants, and collecting at least 50-60 parts of excellent germplasm for local planting and storage; secondly, carrying out salt-tolerant and flooding-tolerant experiments on the collected germplasm, analyzing phenotypic characters and physiological changes of the germplasm, deeply excavating stress-resistant key genes by applying multiple omics technologies such as transcriptome and metabolome, and verifying the expression quantity of the key genes in combination with a qPCR technology; finally, gene expression data and phenotypic resistance analysis results are integrated, salt-tolerant and flood-tolerant mangrove plant varieties rich in genetic background diversity are screened out, a complete technical route of resource collection, omics analysis and directional breeding is formed, and high-quality germplasm resources are provided for mangrove forest ecological restoration.

The present application relates to the technical field of ecological restoration, and discloses a rock drilling and soil covering method for planting mangrove in strongly weathered tuff bottom, aiming to solve the problems of mangrove root system planting difficulty, soil barrenness and high cost of traditional restoration engineering in hard bottom area. The method comprises the following steps: mechanically breaking the surface layer to the average sea level elevation; vibrating and breaking the lower tuff; and backfilling gradient structure planting soil. The present application optimizes the water permeability, continuously supplies fertilizer and buffers the salinity through gradient planting soil design, and reduces the earthwork excavation amount by combining the rock drilling and boring technology, thereby improving the ecological restoration effect of coastal strongly weathered tuff bottom mangrove.

The invention provides a mangrove forest three-dimensional hydrodynamic model construction method and a three-dimensional flow velocity prediction method, and relates to the technical field of ecological systems.The mangrove forest three-dimensional hydrodynamic model construction method comprises the steps that site division is conducted on mangrove forest plaques in a research area, and a plurality of site areas are obtained; acquiring survey data of each site area; the survey data comprises mangrove forest plant distribution characteristics and flow velocity data; determining resistance data items and turbulence energy data items of the mangrove forest to water flow according to the flow velocity data and the mangrove forest plant distribution characteristics; and based on an original momentum equation and a Meller-Yamada model, integrating the resistance data item and the turbulence energy data item, and constructing a mangrove forest three-dimensional hydrodynamic model. The problem that an existing three-dimensional hydrodynamic numerical model lacks a core pain point of an exclusive power module for an intertidal mangrove forest ecosystem is effectively solved, and the complete process of site division, survey data acquisition, resistance and turbulence data calculation and model construction is performed.

The invention belongs to the technical field of ecological restoration and aquaculture, particularly relates to a method for breeding blue crabs in a mangrove forest planting and breeding coupling system, and provides the following scheme for improving the food output and ecological value of the mangrove forest planting and breeding coupling system or an area suitable for mangrove forest breeding. The method comprises the following steps: S1, selecting a proper area as a mangrove forest planting area and a blue crab breeding farm; s2, trimming the selected area, constructing a culture purse seine, and planting mangrove plants and aquatic plants in the purse seine; s3, mussel fertilized eggs or planktonic larvae are introduced in the water inlet process, and meanwhile, artificial fry collection in a sea area is conducted and moved into a culture system; s4, water treatment is conducted, cultured fish, shrimp and crab seeds are put in, and feeding and culturing are started. The mangrove forest ecological system is used for promoting mussel breeding and breeding the blue crabs, the blue crab breeding cost is effectively reduced, the blue crab quality is improved, and protection and restoration of the mangrove forest ecological system are facilitated.

This invention discloses a mangrove planting and protection device and construction method in a fishpond reclamation area. The device includes a settlement adaptation component and a root fixing component. The settlement adaptation component consists of an outer frame, an inner sliding frame, and a drop-reducing unit located at the top. The outer frame is anchored in the reclamation sediment, and a settlement visual marker structure is set on its inner side to indicate the settlement stage of the inner sliding frame. The inner sliding frame can slide vertically along the inner wall of the outer frame to adapt to the sediment settlement. The root fixing component is used to fix the seedlings and provide a growth substrate. The construction method is as follows: the device for planting seedlings is mounted on a drone and accurately placed in the target reclamation area. The device penetrates the soft mud through its bottom structure. During the sediment consolidation and settlement process, the inner sliding frame slides down along the inner wall of the outer frame, achieving synchronous settlement of the roots and the mud surface, effectively preventing root damage and seedling lodging caused by differential settlement.

The invention discloses a mangrove plant kandelia candel seedling cultivation method, and belongs to the field of mangrove plant seedling cultivation methods. The method comprises the four steps of seed pretreatment, seed hormone soaking, seed hormone spraying, seedling hormone foliage spraying and rhizosphere injection. By means of the formula hormone seedling raising method, the germination rate and the germination speed of kandelia candel seeds can be increased, rapid cultivation of kandelia candel seedlings is achieved, a new seedling cultivation thought is provided for kandelia candel intertidal zone transplanting and artificial afforestation, and the formula hormone seedling raising method has important scientific and social significance.

The invention discloses a mangrove forest damage monitoring method and system based on mobile close-range photogrammetry, and the method comprises the steps: determining a target distance through testing a relation value between an image element of a field mobile phone and a distance by means of image recognition; ground control points are set through mobile phone positioning, a coordinate reference system is established based on branch wires, and a high-precision coordinate relation can be established to improve the geographic position of target recognition; a plurality of images of mangrove plants are obtained through the field mobile phone, a seamless synthetic image is output based on a preset mangrove plant image identification AI model, the problem that the view field of the mobile phone is small is solved, and the method is more suitable for large-range monitoring requirements; based on a preset mangrove plant image identification AI model, processing the seamless synthetic image to obtain mangrove plant state information, and identifying the damaged state of the mangrove plant; and obtaining mangrove plant state monitoring information in combination with the coordinate reference system and the plant state information, so that the state of the mangrove plant at a specified position can be accurately identified.

The present application provides a kind of mangrove plants of Bruguiera gymnorrhiza freshwater cultivation method, comprising the following steps: (1) seed treatment: take Bruguiera gymnorrhiza seed, using seed soaking liquid is carried out seed soaking treatment;Seed soaking is carried out after sterilization treatment, dry in shade;Seed soaking liquid is 20-30% of the mass concentration of Veratrum leaf extract;(2) sowing: preparation of seedling substrate, seed is sown in seedling bag containing seedling substrate;The seedling substrate contains sandy loam and livestock manure, fermentation, phosphate fertilizer;Fermentation formula includes: dry cattle manure, Phoenix tree leaf, urea, curved spore bacillus liquid;(3) seedling management.The present application effectively guarantees and promotes Bruguiera gymnorrhiza germination rate, seedling height and survival rate in freshwater environment, germination rate is as high as 97% or more, seedling height reaches 57cm after 6 months of seedling raising, and survival rate reaches 96% or more.

The utility model relates to the field of water treatment, in particular to a tail water treatment system, which comprises a culture pond and a middle filter pond, the middle filter pond comprises a sea horse tooth planting pond and a mangrove forest planting pond, and the culture pond is sequentially communicated with the sea horse tooth planting pond and the mangrove forest planting pond. According to tail water and waste water generated by the culture pond, nitrogen and phosphorus elements in the tail water are absorbed through the sea horse teeth in the sea horse tooth planting pond, the nitrogen and phosphorus elements and heavy metal elements are further absorbed through the large plants in the mangrove forest planting pond, and it is ensured that tail water discharge meets the index; and by adopting an ecological cycle green filtering design, the construction of water treatment equipment can be reduced, and a great deal of cost for purifying the water quality of the tail water is not needed.

The application discloses a method for improving the stability of a coastal wetland mangrove planting substrate soil, which comprises the following steps: taking a mangrove planting substrate soil and reserving; then stirring and adjusting the substrate soil in a sufficient amount of adjusting liquid, and then performing filtration and drying; preparing a chitosan solution according to a weight ratio of 4:9:1 of chitosan, deionized water and a phosphate buffer solution; and fully blending and stirring 4-7 parts of modified rice husk ash, 1-3 parts of sodium humate and 2-4 parts of the chitosan solution with a mass fraction of 5% to obtain a stabilizing modifier. The stability of the mangrove planting substrate soil is improved by adjusting the substrate soil through stirring and adjusting, and then by cooperating with the stabilizing modifier prepared by blending the modified rice husk ash, the sodium humate and the chitosan solution, so that the soil can be optimized in terms of the stability of soil salinization and the stability of soil organic matter content, and the soil property stability is improved.

The invention provides a high-salinity desilting base mud flat modifier and a mangrove forest ecological restoration method, and belongs to the technical field of ecological restoration, the high-salinity desilting base mud flat modifier comprises a multi-layer coated slow-release fertilizer and a sea mud adhesive, the multi-layer coated slow-release fertilizer comprises a compound fertilizer core layer, a seaweed gel coating layer and an organic biochar layer from inside to outside, the compound fertilizer core layer comprises a slow release fertilizer, a microbial preparation and seaweed particles, and the sea mud adhesive comprises dehydrated sea mud and potassium fulvate. The modifier for the high-salinity desilting base mud flat is simple and convenient to construct, environment-friendly and good in application effect; according to the mangrove forest ecological restoration method, the survival rate of mangrove plant seedlings is increased, and manpower and material resource investment of later repeated replanting can be effectively reduced.

The application discloses a system for determining suitable elevation of mangrove restoration in a breeding pond based on numerical simulation of tidal level, and relates to the technical field of marine ecological restoration, and specifically comprises a collection and processing module, a tidal level model module, a scenario simulation module, a relationship analysis module, a screening and comparison module and an optimization decision module. The system first collects basic data; a three-dimensional hydrodynamic numerical model is established based on the principle of hydrodynamics; the water level response under different working conditions is simulated according to the basic data and the operation rules of the gate; through statistical analysis, the daily average flooding time of any given elevation point in the breeding pond within the simulation period is calculated, and a corresponding relationship curve of the elevation at different positions and the daily average flooding time is established; the data of the flooding tolerance time of the target mangrove plant species is taken as a screening condition, and is compared with the corresponding relationship curve; finally, the theoretical suitable elevation range is calculated, and the suitable elevation range determined under the working condition is taken as the basis for guiding the design of the suitable elevation of the mangrove restoration in the breeding pond.

The utility model discloses a kind of fungicide application devices suitable for coastal mudflat mangrove, it is related to plant cultivation technical field, the utility model includes fixed ring, the outside of fixed ring is fixedly connected with several bolt seats, the inner wall of several bolt seats is all rotatably connected with bolt, the outer wall of several bolts is all rotatably connected with bolt seat two, the utility model is provided with rotating wheel, first several bolts are rotated and disassembled, fixed ring can be separated with fixed ring two respectively, then fixed ring and fixed ring two are covered with mangrove trunk in fixed position, then several bolts are reset fixed, it can be achieved that even when mangrove gradually thickens due to growth, the whole equipment can be kept with trunk fixed installation, prevent the installation position of equipment from displacement due to external force factor, and then cause the equipment to continue normal use.

The application discloses a kind of breeding pond mangrove reforestation carbon sink prediction method, it is related to carbon sink evaluation technical field, for solving the problem that prior art cannot accurately predict relevant carbon sink.The method respectively measures mangrove and coastal aquaculture pond carbon dioxide and methane emission and conversion calculation, obtains carbon emission reduction amount;Determination mangrove plant carbon sink and soil carbon burial rate, obtain carbon sink increment;Measure different soil organic carbon storage, calculate the change in reserves.Finally, these three aspects of data, with mangrove seedling recovery 20 years forest conversion, calculate the aquaculture pond mangrove reforestation carbon sink.This method comprehensively considers the carbon emission and soil carbon sink difference ignored by prior art, by innovative calculation method, to accurately evaluate the aquaculture pond mangrove reforestation carbon sink provides an effective way.

The present application relates to the technical field of soil improvement, and discloses a biochar-iron composite material, a preparation method and application thereof.The biochar-iron composite material comprises the following raw materials: iron oxide and mangrove plant biochar.The biochar-iron composite material provided by the present application uses mangrove plant biochar with a large specific surface area and porosity as a raw material, which is conducive to promoting the surface complexation of the biochar-iron composite material and soil organic matter and increasing the adsorption amount of soil organic carbon; the iron oxide is used as a raw material, and iron can be combined with organic carbon through adsorption, coprecipitation or complexation to form a complex with high chemical stability, thereby improving the stability of soil organic carbon; meanwhile, the redox process of iron can inhibit the generation of greenhouse gases, reduce carbon emissions, and significantly enhance the soil carbon preservation function; the biochar-iron composite material is suitable for salinized soil with a salinity of 5 ‰-20 ‰, can effectively inhibit the release of soil CO2, and increase the content of stable organic carbon in soil.

The utility model discloses a mangrove plant hypocotyl planting auxiliary frame, which relates to the technical field of plant cultivation, and comprises a stand column, the outer wall of the stand column is fixedly connected with a disc, the outer wall of one side of the disc garden stand column is fixedly connected with a plurality of insertion rods, and the outer wall of the stand column is provided with a storage mechanism. By means of the supporting plate, when the position is adjusted, the sliding rod is pulled firstly, the spring is squeezed when the sliding rod moves, then the sliding rod is moved out of the fixing groove, the fixing ring is pulled after the sliding rod is moved out, and then the fixing ring drives the supporting plate to slide in the square groove. When the supporting plate moves to a proper position, the sliding rod is loosened, and then the spring extrudes the sliding rod, so that the flexibility of the equipment is improved, the length of the equipment can be adjusted according to different planting densities, the situation that the equipment cannot fix the hypocotyls due to overlong planting spacing is avoided, meanwhile, the mangrove hypocotyls are prevented from being washed away by sea waves, and the survival rate of the mangrove hypocotyls is improved.

This invention relates to the field of water conservancy and hydropower engineering, and particularly to a method for assessing the ecological water requirement of mangrove plants. The method includes: selecting the *Prunus mume* species with the highest area proportion in the mangrove community as the target species within a target area; determining the habitat requirements of the target species during sensitive growth and development stages, and selecting factors sensitive to flow rate changes as habitat suitability indicators, including salinity and immersion time; constructing a three-dimensional temperature-salinity coupled hydrodynamic model, including three-dimensional hydrodynamic control equations, temperature transport control equations, and salinity transport control equations, to simulate the salinity and immersion time in the target species' distribution area under different flow rate conditions; and based on the simulated salinity and immersion time results, combined with a pre-set threshold range for habitat suitability indicators, using the flow rate corresponding to the upper limit of the threshold range as the ecological water requirement of the mangrove plants.

This application relates to the technical field of visual recognition, and in particular to a method for detecting frost damage to mangrove plants based on visual recognition. The method includes acquiring an image sequence; extracting the main vein of the leaf in the first frame as an anchor point; calculating the transformation matrix of subsequent frames relative to the anchor point to obtain a registered image sequence; calculating the temporal variance of the registered image sequence to generate a static baseline image; extracting the leaf outer contour and the connected domain of the lesion to generate a distance gradient field; calculating the mean distance of the connected domain of the lesion in the distance gradient field and the area ratio of the connected domain in the outermost gradient band, and extracting the morphological features of the connected domain of the lesion, thereby dividing the connected domain of the lesion into saline-alkali withered areas or frost damage candidate areas; cropping image blocks corresponding to the frost damage candidate areas from the static baseline image after removing the saline-alkali withered areas, inputting them into an artificial neural network to extract features, and outputting the damage level. This application aims to effectively distinguish between saline-alkali withering and true frost damage to mangrove plants by decoupling and removing dynamic water wave artifacts through spatiotemporal feature decoupling.

The invention discloses a composition for promoting growth of mangrove plants in a high-salinity mud flat and application, and relates to the field of ecological restoration. The composition comprises a slow-release composition A, a slow-release composition B, a microbial preparation and a porous adsorption material, each of the slow-release composition A and the slow-release composition B comprises a basic nutrient layer, a PU hydrogel film layer and a solid powder coating layer; wherein the difference between the sustained-release composition A and the sustained-release composition B is that the PU hydrogel film layer is different. The slow-release composition A and the slow-release composition B which are prepared from two specific raw materials and have different release speeds are matched with the microbial preparation and the porous adsorption material, so that growth of mangrove plants in a seedling raising stage and a transplanting stage can be effectively promoted, and the problem of root burning of the mangrove plants in a high-salt environment is solved; and the survival rate of mangrove plants in a high-salt environment is improved.

The invention discloses a methylophilus and an application thereof in plant growth promotion and biocontrol. The strain has been preserved in GDMCC, and the number of the strain is No.67413. The strain SCSIO43977 can grow by taking methanol as a unique carbon source and energy source, and has the plant growth promoting function of an iron-producing carrier; meanwhile, the bacillus subtilis has the characteristic of antagonizing phytopathogens such as fusarium oxysporum and colletotrichum gloeosporioides, and can be applied to biological control of plant diseases. The strain SCSIO43977 can improve the root dry weight of mangrove plant avicennia marina seeds, promote lateral root growth and effectively reduce the mold infection rate of the seeds, and has important application potential and ecological effects in protection, biocontrol and restoration work of mangrove forest ecological systems.