The invention discloses a
rapid detection method for 27
steroid hormones in
mangrove plants. The method comprises the following steps: S1, putting a
mangrove plant sample subjected to
freeze drying,
grinding and sieving into a centrifugal tube A, and then adding an
isotope internal standard for balancing; adding
acetonitrile, carrying out vortex, ultrasonic extraction and
centrifugation, and taking supernate; s2,
methanol and
acetonitrile are sequentially added, vortex, ultrasonic extraction and
centrifugation are performed, and supernate obtained after
acetonitrile extraction in the S1, supernate obtained after
methanol extraction in the S2 and supernate obtained after acetonitrile extraction in the S2 are combined; s3, transferring the supernate into a centrifugal tube B containing filler, carrying out vortex
centrifugation, and putting the supernate into a glass
test tube; s4, extracting the filler in S3 again by using acetonitrile, carrying out vortex centrifugation, and combining the two supernatants in a glass
test tube; s5, carrying out
nitrogen blowing concentration on the solution in the glass
test tube, and fixing the volume in
methanol; s6, determining the
steroid hormones and the metabolites thereof by using
high performance liquid chromatography-
tandem mass spectrometry, and obtaining the content level of the
steroid hormones and the metabolites thereof in the plants through
data analysis and quantitative calculation.