Seed preservation method of cryptophytic mangrove plant avicennia marina

Abstract

The invention discloses a seed preservation method of cryptophytic mangrove plant avicennia marina. The method comprises the following steps: (1) selecting healthy and full avicennia marina seeds without plant diseases and insect pests, removing outer skin, soaking and disinfecting in a carbendazim aqueous solution for 15 minutes, washing with clear water, and airing; (2) soaking the avicennia marina seeds treated in the step (1) in a chitosan solution for 1 min, taking out, and airing; and (3) embedding the avicennia marina seeds treated in the step (2) into a preservation matrix by taking wet sand as the preservation matrix, then putting the seeds and the preservation matrix into a plastic package bag for sealing, so that the seeds are still kept in a buried state in the bag, and finally storing in a dark environment at 14 DEG C. The germination rate of the avicennia marina seeds stored by the method can still reach 40% after 4 months, the storage time of the avicennia marina seeds is remarkably prolonged, and convenience is provided for seedling raising and ecological restoration of the avicennia marina.

Description

Technical Field

[0001] This invention belongs to the field of plant seed preservation technology, specifically relating to a method for preserving the seeds of the cryptoviviparous mangrove plant Avicennia marina. Background Technology

[0002] Mangroves are a unique plant community dominated by mangrove plants that grows in tropical and subtropical coastal areas. They are mainly distributed in the intertidal zone between the high and low tide lines, growing on mudflats and shallow waters where land and sea meet, forming a unique coastal ecosystem. However, due to indiscriminate sewage discharge, littering on beaches, frequent disease outbreaks, and the development of mariculture, the area of ​​mangroves in China has declined. Therefore, using artificial seedling cultivation for mangrove ecological restoration has become an important remedial measure.

[0003] White bone soil ( Avicennia marina Avicennia marina (also known as White Avicennia) is one of the pioneer tree species for mangrove restoration and reconstruction in my country. It has a well-developed root system, grows rapidly, and is highly adaptable to harsh environments, commonly found in silty or sandy soils, and can be distributed near the low tide line. Avicennia marina seeds are buoyant and can be randomly distributed with ocean currents. They have strong soil nutrient absorption capacity and are suitable for barren sandy tidal flats. However, once the seeds lose water, they rapidly lose viability and begin to mold and rot within 10-20 days at room temperature, posing a significant obstacle to cross-regional introduction, seasonal seedling cultivation, and germplasm preservation. Therefore, finding a method for preserving Avicennia marina seeds and extending their preservation time is crucial for mangrove ecological restoration.

[0004] Currently, there are no relevant methods for preserving Avicennia marina seeds. This patent describes a method that extends the survival time of Avicennia marina seeds by applying a preservative, sealing them in sand, and finally storing them at a low temperature in the dark, thus facilitating subsequent seedling cultivation and planting. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preserving the seeds of the cryptovivobryonic mangrove plant, Avicennia marina. Through disinfection, preservative treatment, and low-temperature sand burial, the preservation time of Avicennia marina seeds is extended, preventing rapid seed rot from affecting seedling cultivation and ecological restoration. It also enables cross-regional transportation and cross-seasonal planting of the seeds.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: A method for preserving the seeds of the cryptovivid mangrove plant Avicennia marina includes the following steps: (1) Select healthy, plump, disease-free Avicennia marina seeds, remove the outer skin, soak them in a carbendazim solution for 15 minutes for disinfection, then rinse them with clean water and let them air dry. (2) After soaking the white mangrove seeds treated in step (1) in a preservative for 1 minute, remove them and air dry; (3) Bury the white mangrove seeds treated in step (2) into the preservation substrate, then put the seeds together with the preservation substrate into a plastic bag and seal it so that the seeds remain buried in the bag. Finally, store them in a dark environment. Furthermore, in step (1), the concentration of the carbendazim aqueous solution is 2 g / L, and the soaking temperature is 25°C; Furthermore, in step (2), the preservative is a chitosan solution, and the soaking temperature is 25°C; Furthermore, the chitosan solution is prepared by dissolving 15g of chitosan powder in a 1vol% aqueous acetic acid solution, adding glycerol with a final concentration of 1vol%, mixing evenly to obtain a chitosan solution. Furthermore, in step (3), the preservation substrate is moist sand, and the preservation temperature is 14°C; Furthermore, the moisture content of the sand is 60%.

[0007] The above-mentioned seed preservation method is applied to the seed preservation of the cryptogenic mangrove plant Avicennia marina.

[0008] The above-mentioned seed preservation method is applied to the artificial seedling cultivation of the cryptogenic mangrove plant Avicennia marina.

[0009] The beneficial effects of this invention are as follows: The method of this invention eliminates the dependence of Avicennia marina seed germination on the seed maturity period. The germination rate of preserved Avicennia marina seeds can still reach 40% after 4 months, enabling cross-seasonal planting of Avicennia marina. Detailed Implementation

[0010] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0011] Example 1: Effect of different storage temperatures on the germination of Avicennia marina seeds when sand is used as the storage substrate (1) Select healthy, plump, and disease-free fresh mature Avicennia marina seeds, remove the outer skin, soak them in a 2 g / L carbendazim aqueous solution for 15 min for disinfection at a temperature of 25°C, then rinse them with clean water and place them in a cool and ventilated place to dry until there are no liquid droplets on the surface, thus obtaining the pretreated Avicennia marina seeds.

[0012] (2) Set up four storage temperature gradients of 4℃, 9℃, 14℃ and 17℃. The other storage conditions are uniform as follows: use sand with a humidity of 60% as storage substrate, bury the pretreated Avicennia marina seeds in the storage substrate, then put the seeds together with the storage substrate into a plastic bag and seal it, so that the seeds are still buried in the bag, and finally store them in a light-proof environment at the corresponding temperature.

[0013] During storage, seeds were taken out every month for germination experiments. The specific steps were as follows: seeds were sown in the holes of a seedling tray filled with substrate (sand), thoroughly watered, and then germination culture was carried out. The germination culture conditions were: the light source was a ZK-TB18-VE02 / A model lamp tube manufactured by Sanan Optoelectronics, with a light intensity of 300 μmol·m⁻¹. -2 ·s -1 The light duration was 18 hours per day; the ambient temperature was 28℃; and the relative humidity was maintained at 70%~90%. Regular watering was performed to keep the substrate in the seedling trays moist but not waterlogged. After 30 days of germination culture, the germination rate was calculated. Germination rate (%): (Number of all germinated seeds within the specified period (30 days) / Number of tested seeds) × 100%.

[0014] Table 1. Effects of different storage temperatures on the germination of Avicennia marina seeds when sand is used as the storage substrate.

[0015] As shown in Table 1, when sand is used as the storage substrate, the Avicennia marina seeds have the longest storage time when the storage temperature is 14℃. After 4 months of storage, there is still a 20% germination rate. However, when the storage time is extended to 5 months and 6 months, the germination rate drops to 0%.

[0016] Example 2: Effect of different storage temperatures on the germination of Avicennia marina seeds when using superabsorbent polymer as the storage substrate (1) Select healthy, plump, and disease-free fresh mature Avicennia marina seeds, remove the outer skin, soak them in a 2 g / L carbendazim aqueous solution for 15 min for disinfection at a temperature of 25°C, then rinse them with clean water and place them in a cool and ventilated place to dry until there are no liquid droplets on the surface, thus obtaining the pretreated Avicennia marina seeds.

[0017] (2) Four storage temperature gradients were set at 4℃, 9℃, 14℃ and 17℃. The remaining storage conditions were uniform as follows: a superabsorbent polymer (Xuzhou Fuxing New Material Technology Co., Ltd., item number SAP-018) with a humidity of 60% was used as the storage substrate. The pretreated Avicennia marina seeds were buried in the storage substrate. Then the seeds and the storage substrate were put into a plastic bag and sealed. The seeds were kept buried in the bag. Finally, they were stored in a light-proof environment at the corresponding temperature.

[0018] During the storage period, the seeds were taken out every month for germination experiments, and the specific steps were the same as in Example 1.

[0019] Table 2. Effects of different storage temperatures on the germination of Avicennia marina seeds when using superabsorbent polymer as the storage substrate.

[0020] As shown in Table 2, when using superabsorbent polymer as the preservation substrate, the Avicennia marina seeds had the longest preservation time when the preservation temperature was 17℃, with a germination rate of 20% after 2 months of preservation. However, when the preservation time was extended to 3 months and 4 months, the germination rate dropped to 0%.

[0021] Example 3: Effects of different preservatives on the germination of Avicennia marina seeds (1) Select healthy, plump, and disease-free fresh mature Avicennia marina seeds, remove the outer skin, soak them in a 2 g / L carbendazim aqueous solution for 15 min for disinfection at a temperature of 25°C, then rinse them with clean water and place them in a cool and ventilated place to dry until there are no liquid droplets on the surface, thus obtaining the pretreated Avicennia marina seeds.

[0022] (2) The pretreated Avicennia marina seeds were treated with different preservatives, as follows: ① Chitosan solution: Dissolve 15g of chitosan powder (molecular weight 10000~50000Da, viscosity 50~800mPa·s, degree of deacetylation 80%~95%) in 1vol% acetic acid aqueous solution, add 1vol% glycerol to the final concentration, mix well to obtain chitosan solution; soak the pretreated Avicennia marina seeds in the chitosan solution for 1min at a soaking temperature of 25℃, then take them out and place them in a cool and ventilated place to dry until there are no liquid droplets on the surface.

[0023] ② Paraffin wax: Soak the pretreated Avicennia marina seeds in melted paraffin wax (melting point 40~50℃) for 1~2 seconds, then remove them and place them in a cool and ventilated place to dry until there are no liquid droplets on the surface.

[0024] ③ Lac solution: Soak the pretreated Avicennia marina seeds in an 8 wt% lac solution (Yunnan Yongde Songhualin Chemical Products Co., Ltd.) for 1-2 seconds at a temperature of 25℃. Then remove them and place them in a cool, ventilated place to dry until there are no liquid droplets on the surface.

[0025] ④ Vaseline: Using the coating method, apply white petroleum jelly evenly to the surface of the pretreated Avicennia marina seeds with a clean, soft brush to form a complete, semi-transparent coating film.

[0026] ⑤ Gelatin solution: Dissolve 1g of gelatin in 50mL of deionized water at 50℃, degas by ultrasonication and filter to obtain a gelatin solution; soak the pretreated Avicennia marina seeds in the gelatin solution for 30s at a temperature of 25℃, then remove them and place them in a cool and ventilated place to dry until there are no liquid droplets on the surface.

[0027] (3) Using sand with a moisture content of 60% as the preservation substrate, the seeds of Avicennia marina treated with preservative were buried in the preservation substrate (Avicennia marina seeds that were only pretreated but not treated with preservative were used as a control). Then, the seeds and the preservation substrate were put into a plastic bag and sealed, so that the seeds remained buried in the bag. Finally, they were stored in a light-proof environment at 14°C.

[0028] During the storage period, the seeds were taken out every month for germination experiments, and the specific steps were the same as in Example 1.

[0029] Table 3 Effects of different preservatives on seed germination of Avicennia marina

[0030] As shown in Table 3, when the preservative is chitosan solution, the seeds of Avicennia marina have the longest preservation time, and still have a germination rate of 40% after 4 months of preservation.

[0031] In summary, soaking Avicennia marina seeds in chitosan solution for 1 minute, then removing and drying them, burying them in sand with a moisture content of 60%, and finally sealing the seeds and sand together in a plastic bag and storing them in the dark at 14℃ are suitable conditions for preserving Avicennia marina seeds.

[0032] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made within the scope of the claims of the present invention should be included in the scope of the present invention.

Claims

1. A method for preserving the seeds of the cryptovivid mangrove plant *Avicennia marina*, characterized by: Includes the following steps: (1) Select healthy, plump, disease-free Avicennia marina seeds, remove the outer skin, soak them in a carbendazim solution for 15 minutes for disinfection, then rinse them with clean water and let them air dry. (2) After soaking the white mangrove seeds treated in step (1) in a preservative for 1 minute, remove them and air dry; (3) Bury the white mangrove seeds treated in step (2) into the preservation substrate, then put the seeds together with the preservation substrate into a plastic bag and seal it, so that the seeds remain buried in the bag, and finally store them in a dark environment.

2. The seed preservation method according to claim 1, characterized in that: In step (1), the concentration of the carbendazim aqueous solution is 2 g / L, and the soaking temperature is 25°C.

3. The seed preservation method according to claim 1, characterized in that: In step (2), the preservative is a chitosan solution, and the soaking temperature is 25°C.

4. The seed preservation method according to claim 3, characterized in that: The chitosan solution is prepared by dissolving 15g of chitosan powder in a 1 vol% aqueous acetic acid solution, adding glycerol with a final concentration of 1 vol%, and mixing thoroughly to obtain the chitosan solution.

5. The seed preservation method according to claim 1, characterized in that: In step (3), the preservation substrate is moist sand, and the preservation temperature is 14°C.

6. The seed preservation method according to claim 5, characterized in that: The moisture content of the sand is 60%.

7. The application of the seed preservation method according to any one of claims 1 to 6 in the seed preservation of the cryptogenic mangrove plant Avicennia marina.

8. The application of the seed preservation method according to any one of claims 1 to 6 in the artificial seedling cultivation of the cryptogenic mangrove plant Avicennia marina.

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