22 results about "Scylla paramamosain" patented technology

The application discloses a SNP molecular marker related to the thickness of a left chelae of Scylla paramamosain, a detection primer pair, a kit and purposes. The SNP molecular marker is that the 184th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the left chelae thickness of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The present application relates to a kind of Scylla paramamosain gender-specific genomic sequences and genetic gender identification method, the gender-specific genomic sequence is as SEQ NO.1 and as SEQ NO.2 shown in sequence;Wherein, genetic gender is female individual genome simultaneously containing SEQ NO.1 and SEQ NO.2 sequence, genetic gender is male individual genome only containing SEQ NO.2 sequence.Simultaneously, the present application also uses the gender-specific sequence to develop the genetic gender identification method based on PCR technology.The present application can be used for the identification of Scylla paramamosain gender-specific large fragment and genetic gender rapid detection, with the advantages of strong specificity, high accuracy, simple operation, low cost etc., can be used for crab sex control breeding industry and gender determination and differentiation mechanism research etc., with wide application prospect.

The invention relates to the technical field of aquaculture feed, in particular to a feed additive for improving ovary color of scylla paramamosain and application of the feed additive. The feed additive is prepared from the following raw material components in parts by mass: 30-60 parts of Chinese wolfberry fruit powder, 10-30 parts of a marigold extract, 2-8 parts of chenodeoxycholic acid and 5-15 parts of vitamin E powder. The additive systematically solves the technical bottlenecks of low absorptivity and unstable deposition of natural pigments in crab bodies through the synergistic effect of all the components. When the feed additive is added into feed for fattening female scylla paramamosain, the ovary redness value can be remarkably increased, the color is rich and natural, and meanwhile, the growth of parent crabs and the development of gonad are promoted. The feed is completely prepared from natural raw materials, is safe, free of residues and controllable in cost, and provides an effective scheme for realizing high-valued and green cultivation of scylla paramamosain'red cream crab '.

The application discloses a scylla paramamosain antibacterial polypeptide Spgilstin 10‑33 and application thereof, a molecular formula of which is C 118 H 206 N 42 O 30 S, and an amino acid sequence of the Spgilstin is shown as SEQ ID NO. 01. The application has broad-spectrum antibacterial and antimycotic activities, high bactericidal efficiency, no cytotoxicity, high safety, and can be applied to antibacterial agents, antimold agents and aquatic feed additives as effective components, and has wide application prospects.

This invention belongs to the field of biotechnology, specifically relating to a non-harmful DNA extraction method for *Scylla serrata* (scuttica crab) and its application. This invention successfully achieves non-harmful DNA extraction from *Scylla serrata* by collecting its swimming leg setae, significantly reducing the impact on the crab's activity and ensuring its survival after sampling. The extraction method of this invention is non-lethal to *Scylla serrata* and possesses good repeatability and high accuracy. Extracting DNA from *Scylla serrata* using its swimming leg setae ensures that the crab's activity remains unchanged, allowing it to survive and perform its life activities normally. Obtaining the DNA sequence of *Scylla serrata* and performing gene markers provides a foundation for genomic selection breeding, facilitating parent selection and offspring breeding in mud crab genetics.

The invention belongs to the technical field of scylla paramamosain aquaculture, and particularly relates to a multi-channel feeding inspection robot for scylla paramamosain industrial aquaculture, which comprises an AGV (Automatic Guided Vehicle) and a main body bracket, the bottom end of the main body bracket is fixedly mounted at the top end of the AGV trolley; a central host is mounted on the main body bracket; a plurality of groups of regulation and control hosts are mounted on the side wall of the main body bracket; a plurality of groups of XYZ linear guide rail modules are mounted on the main body bracket, and the regulation and control host controls the XYZ linear guide rail modules corresponding to the XYZ linear guide rail modules in position; the central host controls the multiple groups of regulation and control hosts and the AGVs; the XYZ linear rail module is provided with a stock bin; a stock bin cover is hinged to the top end of the stock bin; a discharging mechanism is arranged in the stock bin; the invention provides a multi-channel feeding inspection robot for industrial aquaculture of scylla paramamosain. The multi-channel feeding inspection robot is used for solving the problem of low feeding efficiency in the prior art.

The invention discloses a lossless intelligent staging method and system for oocytes of scylla paramamosain. The lossless intelligent staging method comprises the following steps: obtaining morphological parameters of living scylla paramamosain at least including the first length and the body weight; the morphological parameters are input into an XGBoost-based feature enhancement regression prediction model which is subjected to Pipeline packaging training in advance and has optimal parameters, and an oocyte diameter prediction value is obtained; accurately matching a preset staging standard according to the predicted value, and determining an ovarian development staging; and when the prediction confidence coefficient is low, triggering identification and carrying out incremental learning on the model by utilizing confirmation data so as to adaptively optimize the model precision. According to the method, machine learning is used for replacing anatomy to realize nondestructive measurement of the oocyte diameter of the blue crab, and a diameter direct connection ovary development stage table automatic matching development stage is predicted. The simplest configuration of the method only needs the measurement of the nail length and the body weight as effective parameters, the lightweight model can be applied to a quick online lossless ovarian development staging system of the blue crabs, and the intelligent staging can effectively guide the actual breeding decision.

This invention provides a method for constructing an efficient ecological algal community for indoor breeding of mud crabs. Based on the growth characteristics of mud crab zoea larvae and their requirements for water quality and nutrition at each stage, a method for constructing a superior microalgal community and a balanced bacterial-algal community in the mud crab breeding pond is proposed. Six novel algal combinations not previously used in mud crab breeding are used to replace native algae, supplemented with probiotics. A daily combination of algae, bacteria, and biological feed for each developmental stage of the larvae (Z1-C1) is designed, significantly improving the survival rate and efficiency of indoor mud crab breeding. Compared to traditional breeding methods that rely entirely on native algae, this artificially constructed algae is more efficient and reproducible, facilitating the standardization of indoor breeding. This invention promotes a refined management model, achieving a balanced bacterial-algal community, stable and controllable water environment, and avoiding the instability caused by complex and variable native algae. This is more conducive to the promotion of mud crab breeding in areas with poor natural water quality.

The application discloses a SNP molecular marker related to the length of the third right step foot of Scylla paramamosain, a detection primer pair, a kit and purposes. The SNP molecular marker is a nucleotide sequence shown in SEQ ID NO:1, wherein the 108th base from the 5' end is C or A. The SNP molecular marker can be used for predicting the length of the third right step foot of an individual at an early breeding stage, and can be used for molecular marker assisted selection breeding, so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.

The application discloses a scylla paramamosain antibacterial peptide Sparamosin 26‑54 The application has significant antiviral effect on white spot syndrome virus, frog iridovirus and eel herpesvirus, has the characteristics of easy synthesis, stability, high antiviral activity and low toxicity, and has good application prospect in drug research.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker combination related to low-salt resistance of scylla paramamosain and application of the SNP molecular marker combination, and the SNP molecular marker combination comprises nine sites SNP1-SNP9 in total. According to the method, combined genotype analysis is carried out based on multiple SNP sites, genetic information of multiple sites is integrated, and the accuracy of predicting the low-salt-tolerant phenotypic character of scylla paramamosain can be remarkably improved. The SNP molecular marker combination is applied to breeding practice of scylla paramamosain, low-salt-resistant performance screening can be performed on parents and individuals in the seedling stage or the early development stage, and a technical basis is provided for cultivating a novel low-salt-resistant scylla paramamosain strain and supporting stable development of the breeding industry.

The application discloses a SNP molecular marker related to body weight and / or body height of Scylla paramamosain, a detection primer pair, a kit and application. The SNP molecular marker is that the 111th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the body weight and / or body height of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The application discloses a SNP molecular marker related to Scylla paramamosain back carapace width on chromosome 2, a detection primer pair, a kit and purposes. The SNP molecular marker is that the 202th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is A or T. The SNP molecular marker can be used for predicting the back carapace width of an individual in early breeding, and is used for molecular marker assisted selection breeding, so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The invention relates to the field of aquaculture feed, and particularly discloses an anti-stress functional composition, feed as well as a preparation method and application of the anti-stress functional composition. The anti-stress functional composition is prepared from L-theanine, tea polyphenol, a bighead atractylodes rhizome extract and beta-1, 3-glucan. According to the composition, through the synergistic effect of the L-theanine, the tea polyphenol, the rhizoma atractylodis macrocephalae extract and the beta-1, 3-glucan, the anti-stress ability of the scylla paramamosain can be remarkably enhanced, then the survival rate of the scylla paramamosain is increased, the vitality performance of the scylla paramamosain is improved, meanwhile, the long-distance transportation resistance of the scylla paramamosain is enhanced, and a guarantee is provided for subsequent breeding and circulation links. The invention relates to a feed for improving the anti-stress capability of scylla paramamosain and a preparation method thereof, which not only can accurately meet the core demand of the scylla paramamosain for improving the anti-stress capability, but also can solve the problems of unbalanced nutrition, easy carrying of pathogens and water pollution of the existing feed by optimizing a formula and a preparation process.

The invention discloses a temperature-controllable scylla paramamosain fry intermediate cultivation device and method, and belongs to the technical field of crab fry cultivation, the technical key points are that the device comprises a cultivation box, temperature control treatment can be well carried out through an arranged temperature control oxygenation mechanism, meanwhile, tiny bubbles are generated through high-pressure aeration of a bubble generator, and the tiny bubbles are filled in the cultivation box; meanwhile, the rotating base rotates to drive the water body to form an annular circulating flow, a good foundation is laid for the subsequent cultivation stage, the arranged water changing cultivation mechanism achieves mixing of a salt solution or blending of a nutritional agent through water changing operation, and injection of a mixed solution is achieved synchronously; the water is synchronously injected into the breeding pond along with the water changing process, the continuity of nutrition or salinity supply of the juvenile crabs is guaranteed, so that the breeding period is correspondingly shortened, the nutrient is rapidly diffused to the whole pond in combination with the rotating water flow above the bubble generator, and the device has the advantages of convenience in juvenile crab breeding and adjustability, control and treatment.

The application discloses a kind of based on basket culture Scylla paramamosain conservation method, belong to Scylla paramamosain conservation technical field, process includes: the crab seed juvenile crab 1 period mark coarse culture 1 month, select uniform female crab individual size to carry out basket culture, feed in basket living body shellfish, once a week feeds small miscellaneous fish, feeds again, breeds to before reproductive ecdysis;Female crab is mixed with mature male crab in the same pond before reproductive ecdysis, feeds fresh oyster meat, female to male ratio is 1:2, female and male mating again breed 10d, then female crab is hung up in cultivation basket, artificial induced spawning next year, induced spawning salinity 20%~25%.The conservation method of the application promotes mating female crab to develop into paste crab, and the short-term artificial induced spawning of paste crab can reach 25.6%~31.4%, which meets the general commercial seedling raising demand and breeding requirement, solves the problem of poor artificial conservation effect of mud crab breeding, and is suitable for different species source of mud crab original seed protection and commercial use.

The invention relates to the technical field of intelligent bait casting, and discloses an intelligent bait casting method and system for scylla paramamosain seawater fries, and the method comprises the steps: extracting a motion track and distribution characteristics according to a real-time motion image of the scylla paramamosain fries in a culture pond, and obtaining behavior characteristic parameters of the scylla paramamosain fries; when the activity degree of the motion trail in the behavior characteristic parameters exceeds a historical data threshold value, it is judged that the scylla paramamosain seeds are in a real-time starvation state; according to the linear relation between the historical bait casting amount and the historical hunger state, the real-time bait casting amount is determined; controlling a bait casting device to scatter bait according to the real-time bait casting amount; after the bait distribution is completed, acquiring the feeding image of the offspring seeds again, and analyzing the feeding image of the offspring seeds, so as to obtain feedback data of the distributed bait; based on the feedback data, the linear relation is dynamically adjusted, and the real-time bait casting amount of the bait casting device is updated according to the dynamically-adjusted linear relation; the intelligent bait casting efficiency can be improved.

The application belongs to the technical field of exosomes, and discloses a preparation method of artificial exosomes of blood cells of a crustacean Scylla paramamosain, which mainly comprises the following steps: 1) collecting blood cells of the Scylla paramamosain by centrifugation; 2) collecting cell membranes and cytoplasmic matrix respectively after blood cells are broken and centrifuged; and 3) obtaining artificial exosomes under certain reaction conditions. The exosomes obtained by the production method can be used as a carrier, and by carrying RNA or proteins, can be widely used in immunological research and disease prevention and treatment of the crustacean.

The invention relates to the related field of crab culture, in particular to a scylla paramamosain whole-process factory culture technology which comprises the following steps: preparing a culture cement pond; selecting juvenile crabs and treating the juvenile crabs before entering the pond; and daily breeding management. The whole-process culture of the seawater blue crabs under the indoor factory condition is realized for the first time, a new culture variety is provided for factory facilities, and the culture space can be effectively expanded and the total culture yield can be increased under the situation that the coastal mud flat area required by the current seawater crab culture is shrunk; according to the method, the required culture water body area is small, the unit culture density is high, culture conditions are controllable in the whole process, daily management is convenient, the method has remarkable advantages in the aspects of water quality regulation and control, bait utilization, disease prevention and control, natural disaster response and the like, and the culture survival rate is increased to 40% or above from 20% or below of a traditional method; the breeding density is increased to 5-10 pieces / m < 2 > from 3 pieces / m < 2 > or below; the cultivation yield per mu is increased from 50 kg to 460 kg, and the cultivation benefits are remarkably improved.

The invention relates to a green crab seed intermediate cultivation method suitable for northern saline-alkali soil. The green crab seed intermediate cultivation method is characterized by comprising the following steps: (1) breeding environment transformation before stocking; (2) culturing in stages C1 to C2; compared with the prior art, the crab breeding method has the following advantages that the survival rate of the fry is greatly increased; 2, the breeding period is obviously shortened; 3, the breeding cost is reduced; 4, the culture region and economic benefit are expanded: the culture bottleneck of the scylla paramamosain in northern saline-alkali soil is successfully broken through, the culture region is expanded to an inland saline-alkali region by hundreds of kilometers, and the newly increased culture area reaches millions of mu.

The application discloses a SNP molecular marker related to Scylla paramamosain trunk weight, a detection primer pair, a kit and purposes. The SNP molecular marker is that the 93th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the trunk weight of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.