35 results about "Scylla paramamosain" patented technology

The invention relates to the technical field of ecological breeding of scylla paramamosain and protection and restoration of mangrove forests. The invention provides a method for culturing scylla paramamosain by using mangrove forest, which comprises the following steps: (1) carrying out simple engineering transformation on the basis of a coastal wetland traditional culture pond, constructing a planting island in the middle of the culture pond, and reserving an annular tidal creek between the planting island and the periphery of the culture pond; (2) planting mangrove plants in the planting island to form a man-made forest; (3) small aquatic plants are planted at the bottom of the artificial forest, and large algae are planted in the annular ditches; and (4) putting the young blue crabs into the culture pond, and carrying out daily management until the blue crabs are harvested. The shellfish is used for providing main food supply for the blue crabs, and meanwhile water is purified; excrement of the blue crabs can be fed back to promote growth of mangroves, algae, mussels and the like; large algae can perform photosynthesis at the bottom of the pond to increase dissolved oxygen in water, and small sea grass is placed in the planting island to make up for the defect of low coverage of newly planted mangrove forest plant seedlings.

The application discloses a SNP molecular marker related to the thickness of a left chelae of Scylla paramamosain, a detection primer pair, a kit and purposes. The SNP molecular marker is that the 184th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the left chelae thickness of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The present application relates to a kind of Scylla paramamosain gender-specific genomic sequences and genetic gender identification method, the gender-specific genomic sequence is as SEQ NO.1 and as SEQ NO.2 shown in sequence;Wherein, genetic gender is female individual genome simultaneously containing SEQ NO.1 and SEQ NO.2 sequence, genetic gender is male individual genome only containing SEQ NO.2 sequence.Simultaneously, the present application also uses the gender-specific sequence to develop the genetic gender identification method based on PCR technology.The present application can be used for the identification of Scylla paramamosain gender-specific large fragment and genetic gender rapid detection, with the advantages of strong specificity, high accuracy, simple operation, low cost etc., can be used for crab sex control breeding industry and gender determination and differentiation mechanism research etc., with wide application prospect.

A non-injury detection method for the ovary development stage of Scylla paramamosain, comprising the following steps: obtaining a fitting curve equation with the ovary development stage number as the variable and the cross-sectional area ratio of ovary to carapace as the dependent variable; screening individuals to be detected that meet the conditions; performing anesthesia pretreatment; placing the individual to be detected under a light source to irradiate it so that the shadow profile of the ovary appears on the carapace; obtaining an image including the carapace under the irradiation of the light source; obtaining the area value of the shadow profile of the ovary on the carapace and the image area value of the carapace in the image respectively, and calculating the ratio of the two; substituting the ratio as the dependent variable into the fitting curve equation to obtain the variable corresponding to the dependent variable, and rounding the variable to an integer to obtain the ovary development stage number of the female Scylla paramamosain individual. The present application can avoid causing body damage to the detected Scylla paramamosain, and ensure that the detected Scylla paramamosain continues to be used for production and sales and fry breeding.

The invention relates to the technical field of aquaculture feed, in particular to a feed additive for improving ovary color of scylla paramamosain and application of the feed additive. The feed additive is prepared from the following raw material components in parts by mass: 30-60 parts of Chinese wolfberry fruit powder, 10-30 parts of a marigold extract, 2-8 parts of chenodeoxycholic acid and 5-15 parts of vitamin E powder. The additive systematically solves the technical bottlenecks of low absorptivity and unstable deposition of natural pigments in crab bodies through the synergistic effect of all the components. When the feed additive is added into feed for fattening female scylla paramamosain, the ovary redness value can be remarkably increased, the color is rich and natural, and meanwhile, the growth of parent crabs and the development of gonad are promoted. The feed is completely prepared from natural raw materials, is safe, free of residues and controllable in cost, and provides an effective scheme for realizing high-valued and green cultivation of scylla paramamosain'red cream crab '.

The invention discloses a counting device for large-size fries of scylla paramamosain and a using method of the counting device, and relates to the technical field of aquaculture equipment. The counting equipment adopts a modular design and comprises a conveying system, a counting system, a juvenile crab throwing box and a juvenile crab box, a tail baffle of the juvenile crab throwing box can move back and forth according to the number of juvenile crabs; a driving motor of the conveying system is arranged at one end far away from the juvenile crab throwing box to prevent water dripped and splashed from the juvenile crab device from damaging the driving motor, the conveying system is embedded with a double-leaf door of the juvenile crab throwing box, and juvenile crabs in the juvenile crab throwing box are conveyed one by one through a conveying belt, pass through the counting system and enter the juvenile crab box after counting; counting equipment adopts an infrared digital display system and is fixed on a supporting plate on one side, close to a driving motor, of the conveying system, the juvenile crabs on a conveying belt are counted after passing through a scanning recognition area, a height limiting baffle with adjustable height is arranged at a double-leaf door, the juvenile crabs can pass through the counting system one by one, and the counting accuracy is improved.

The application discloses a scylla paramamosain antibacterial polypeptide Spgilstin 10‑33 and application thereof, a molecular formula of which is C 118 H 206 N 42 O 30 S, and an amino acid sequence of the Spgilstin is shown as SEQ ID NO. 01. The application has broad-spectrum antibacterial and antimycotic activities, high bactericidal efficiency, no cytotoxicity, high safety, and can be applied to antibacterial agents, antimold agents and aquatic feed additives as effective components, and has wide application prospects.

The invention discloses a specific SNP molecular marker for sex identification of scylla paramamosain as well as a detection reagent and application of the specific SNP molecular marker, the SNP molecular marker is located at the 601st site of a nucleotide sequence SEQ ID NO: 1, and the sex of scylla paramamosain can be simply, rapidly, effectively and accurately identified according to the SNP molecular marker. The invention further provides a detection reagent of the SNP molecular marker, and the detection reagent can be applied to early auxiliary screening of scylla paramamosain, so that the breeding time of scylla paramamosain is shortened, and the breeding process is accelerated.

This invention belongs to the field of biotechnology, specifically relating to a non-harmful DNA extraction method for *Scylla serrata* (scuttica crab) and its application. This invention successfully achieves non-harmful DNA extraction from *Scylla serrata* by collecting its swimming leg setae, significantly reducing the impact on the crab's activity and ensuring its survival after sampling. The extraction method of this invention is non-lethal to *Scylla serrata* and possesses good repeatability and high accuracy. Extracting DNA from *Scylla serrata* using its swimming leg setae ensures that the crab's activity remains unchanged, allowing it to survive and perform its life activities normally. Obtaining the DNA sequence of *Scylla serrata* and performing gene markers provides a foundation for genomic selection breeding, facilitating parent selection and offspring breeding in mud crab genetics.

The invention belongs to the technical field of scylla paramamosain aquaculture, and particularly relates to a multi-channel feeding inspection robot for scylla paramamosain industrial aquaculture, which comprises an AGV (Automatic Guided Vehicle) and a main body bracket, the bottom end of the main body bracket is fixedly mounted at the top end of the AGV trolley; a central host is mounted on the main body bracket; a plurality of groups of regulation and control hosts are mounted on the side wall of the main body bracket; a plurality of groups of XYZ linear guide rail modules are mounted on the main body bracket, and the regulation and control host controls the XYZ linear guide rail modules corresponding to the XYZ linear guide rail modules in position; the central host controls the multiple groups of regulation and control hosts and the AGVs; the XYZ linear rail module is provided with a stock bin; a stock bin cover is hinged to the top end of the stock bin; a discharging mechanism is arranged in the stock bin; the invention provides a multi-channel feeding inspection robot for industrial aquaculture of scylla paramamosain. The multi-channel feeding inspection robot is used for solving the problem of low feeding efficiency in the prior art.

The invention discloses a lossless intelligent staging method and system for oocytes of scylla paramamosain. The lossless intelligent staging method comprises the following steps: obtaining morphological parameters of living scylla paramamosain at least including the first length and the body weight; the morphological parameters are input into an XGBoost-based feature enhancement regression prediction model which is subjected to Pipeline packaging training in advance and has optimal parameters, and an oocyte diameter prediction value is obtained; accurately matching a preset staging standard according to the predicted value, and determining an ovarian development staging; and when the prediction confidence coefficient is low, triggering identification and carrying out incremental learning on the model by utilizing confirmation data so as to adaptively optimize the model precision. According to the method, machine learning is used for replacing anatomy to realize nondestructive measurement of the oocyte diameter of the blue crab, and a diameter direct connection ovary development stage table automatic matching development stage is predicted. The simplest configuration of the method only needs the measurement of the nail length and the body weight as effective parameters, the lightweight model can be applied to a quick online lossless ovarian development staging system of the blue crabs, and the intelligent staging can effectively guide the actual breeding decision.

This invention provides a method for constructing an efficient ecological algal community for indoor breeding of mud crabs. Based on the growth characteristics of mud crab zoea larvae and their requirements for water quality and nutrition at each stage, a method for constructing a superior microalgal community and a balanced bacterial-algal community in the mud crab breeding pond is proposed. Six novel algal combinations not previously used in mud crab breeding are used to replace native algae, supplemented with probiotics. A daily combination of algae, bacteria, and biological feed for each developmental stage of the larvae (Z1-C1) is designed, significantly improving the survival rate and efficiency of indoor mud crab breeding. Compared to traditional breeding methods that rely entirely on native algae, this artificially constructed algae is more efficient and reproducible, facilitating the standardization of indoor breeding. This invention promotes a refined management model, achieving a balanced bacterial-algal community, stable and controllable water environment, and avoiding the instability caused by complex and variable native algae. This is more conducive to the promotion of mud crab breeding in areas with poor natural water quality.

The application discloses a SNP molecular marker related to the length of the third right step foot of Scylla paramamosain, a detection primer pair, a kit and purposes. The SNP molecular marker is a nucleotide sequence shown in SEQ ID NO:1, wherein the 108th base from the 5' end is C or A. The SNP molecular marker can be used for predicting the length of the third right step foot of an individual at an early breeding stage, and can be used for molecular marker assisted selection breeding, so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.

The application discloses a scylla paramamosain antibacterial peptide Sparamosin 26‑54 The application has significant antiviral effect on white spot syndrome virus, frog iridovirus and eel herpesvirus, has the characteristics of easy synthesis, stability, high antiviral activity and low toxicity, and has good application prospect in drug research.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker combination related to low-salt resistance of scylla paramamosain and application of the SNP molecular marker combination, and the SNP molecular marker combination comprises nine sites SNP1-SNP9 in total. According to the method, combined genotype analysis is carried out based on multiple SNP sites, genetic information of multiple sites is integrated, and the accuracy of predicting the low-salt-tolerant phenotypic character of scylla paramamosain can be remarkably improved. The SNP molecular marker combination is applied to breeding practice of scylla paramamosain, low-salt-resistant performance screening can be performed on parents and individuals in the seedling stage or the early development stage, and a technical basis is provided for cultivating a novel low-salt-resistant scylla paramamosain strain and supporting stable development of the breeding industry.

The application discloses a SNP molecular marker related to body weight and / or body height of Scylla paramamosain, a detection primer pair, a kit and application. The SNP molecular marker is that the 111th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the body weight and / or body height of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The invention discloses a reagent for detecting SNP (Single Nucleotide Polymorphism) locus genotypes of salt-tolerant related traits of scylla paramamosain and application of the reagent. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a scylla paramamosain genome. The SNP sites comprise an SNP site 1, an SNP site 2, an SNP site 3 and an SNP site 4; the SNP site 1 is located at the 13938th basic group of the CA gene, the SNP site 2 is located at the 13981th basic group of the CA gene, the SNP site 3 is located at the 18286th basic group of the CA gene, and the SNP site 4 is located at the 18336th basic group of the CA gene. According to the invention, the single nucleotide polymorphism (SNP) of the salt tolerance related characters of the scylla paramamosain character is screened, the SNP is identified through sequencing, the peak map is clear, the typing is reliable, the salt tolerance related characters of the scylla paramamosain character are effectively determined, and valuable information is provided for breeding.

The application discloses a SNP molecular marker related to Scylla paramamosain back carapace width on chromosome 2, a detection primer pair, a kit and purposes. The SNP molecular marker is that the 202th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is A or T. The SNP molecular marker can be used for predicting the back carapace width of an individual in early breeding, and is used for molecular marker assisted selection breeding, so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The invention belongs to the technical field of male and female fry sorting, and discloses male and female sorting equipment for large-size scylla paramamosain fries. Comprising a juvenile crab throwing box, a support cross beam, a movable tail plate, a double-leaf door type switch, a support leg structure, an infrared counter, a juvenile crab box, an adjustable height limiting plate, a transparent crawler belt, an image acquisition system, a data processing and shifting piece control system, a data line, a movable shifting piece and a movable separating plate. After analysis of the image acquisition system and the data analysis system, when the individual sex cannot be distinguished due to the conditions that images are fuzzy or measurement parts are shielded, the movable separation plate is controlled by the controller and rapidly and obliquely moves upwards, so that the juvenile crabs slide into the juvenile crab box with the head close to the ground along the baffle after being collected on the movable separation plate, and the juvenile crabs are separated from the juvenile crabs. And then the collected juvenile crabs are distinguished again in a manner of throwing the juvenile crabs again or manually sorting the juvenile crabs.

The invention relates to the field of aquaculture feed, and particularly discloses an anti-stress functional composition, feed as well as a preparation method and application of the anti-stress functional composition. The anti-stress functional composition is prepared from L-theanine, tea polyphenol, a bighead atractylodes rhizome extract and beta-1, 3-glucan. According to the composition, through the synergistic effect of the L-theanine, the tea polyphenol, the rhizoma atractylodis macrocephalae extract and the beta-1, 3-glucan, the anti-stress ability of the scylla paramamosain can be remarkably enhanced, then the survival rate of the scylla paramamosain is increased, the vitality performance of the scylla paramamosain is improved, meanwhile, the long-distance transportation resistance of the scylla paramamosain is enhanced, and a guarantee is provided for subsequent breeding and circulation links. The invention relates to a feed for improving the anti-stress capability of scylla paramamosain and a preparation method thereof, which not only can accurately meet the core demand of the scylla paramamosain for improving the anti-stress capability, but also can solve the problems of unbalanced nutrition, easy carrying of pathogens and water pollution of the existing feed by optimizing a formula and a preparation process.

The invention discloses a breeding method of scylla paramamosain, and particularly relates to a method for improving the egg carrying rate of scylla paramamosain under the condition of factory circulating water culture, which comprises the following specific steps: 1) selecting mated female crabs with complete and healthy appendages, the weight of which is 400-500g, the gonad development reaches IV stage or above, and the ovary index is greater than 12%; (2) carrying out hot burning ablation on single-side eyestalks of the crab parents; and (3) controlling environmental parameters and strengthening baits during the breeding period of the crab parents. According to the method, the technical problem that the egg carrying rate is limited in artificial breeding of scylla paramamosain is effectively solved. According to the method for improving the egg carrying rate of the scylla paramamosain under the factory-like circulating water culture condition, the egg carrying rate of the scylla paramamosain can be remarkably improved through culture environment parameter setting, bait strengthening and single-side eyestalk ablation operation adopting a hot burning ablation method, namely, the reproductive capacity of the scylla paramamosain can be effectively promoted, and the survival rate of the scylla paramamosain is improved. Furthermore, the breeding efficiency of the scylla paramamosain is improved under the factory circulating water breeding condition.

The invention discloses a temperature-controllable scylla paramamosain fry intermediate cultivation device and method, and belongs to the technical field of crab fry cultivation, the technical key points are that the device comprises a cultivation box, temperature control treatment can be well carried out through an arranged temperature control oxygenation mechanism, meanwhile, tiny bubbles are generated through high-pressure aeration of a bubble generator, and the tiny bubbles are filled in the cultivation box; meanwhile, the rotating base rotates to drive the water body to form an annular circulating flow, a good foundation is laid for the subsequent cultivation stage, the arranged water changing cultivation mechanism achieves mixing of a salt solution or blending of a nutritional agent through water changing operation, and injection of a mixed solution is achieved synchronously; the water is synchronously injected into the breeding pond along with the water changing process, the continuity of nutrition or salinity supply of the juvenile crabs is guaranteed, so that the breeding period is correspondingly shortened, the nutrient is rapidly diffused to the whole pond in combination with the rotating water flow above the bubble generator, and the device has the advantages of convenience in juvenile crab breeding and adjustability, control and treatment.

The present invention provides a method for artificially cultivating soft-shell crabs, wherein the method comprises adjusting the bicarbonate ion HCO3 in the aquaculture water when cultivating the soft-shell crabs. ‑ The concentration of calcium ions Ca in the aquaculture water is 400±20 mg / L; further, the method of the present invention controls the calcium ions Ca in the aquaculture water during the aquaculture period. 2+ The concentration is not less than 120mg / L; control the magnesium ion Mg in the aquaculture water 2+ Concentration not less than 200mg / L; potassium ion K + The concentration is not less than 100 mg / L; the total hardness of the aquaculture water is controlled to be not less than 800 mg / L; the pH of the aquaculture water is controlled to be 7.8-8.6. The method of the present invention is to adjust the bicarbonate ion HCO3 in the water ‑ The concentration of the salt causes the hardened crab shells to soften and become soft-shell crabs without molting, thus increasing the economic benefits of aquaculture.

The application belongs to the technical field of Scylla paramamosain, and particularly relates to a method for promoting Scylla paramamosain to hold eggs. The method comprises the following steps: domesticating Scylla paramamosain by using water bodies with the same salinity as that of the water bodies in a pond for breeding Scylla paramamosain; stimulating the domesticated Scylla paramamosain by using low-temperature and low-salinity water bodies; and inducing the stimulated Scylla paramamosain by using high-temperature and high-salinity water bodies. The Scylla paramamosain is sequentially domesticated, stimulated and induced, and the sudden change of water temperature and salinity is continuously controlled, so that the Scylla paramamosain can efficiently hold eggs.

The invention belongs to the technical field of aquatic organisms, and particularly relates to dsRNA interference primers for regulating a sex gene Dmrta2 of blue crabs as well as a method and application of the dsRNA interference primers. The invention provides dsRNA (double-stranded ribonucleic acid) for regulating and controlling a sex gene of blue crab Dmrta2. The dsRNA is obtained by taking a spliceosome sequence as a template through in-vitro transcription; the invention also provides an RNA interference method for regulating and controlling testis development of scylla paramamosain by using the Dmrta2 gene variable spliceosome. The method comprises in-vivo interference treatment and in-vitro interference treatment. The result of the embodiment shows that the RNA interference is implemented in the in-vivo and in-vitro tissue culture of the scylla paramamosain by the method, the expression interference of the Dmrta2 gene variable spliceosome can be successfully completed, and the retardation or abnormality of sperm generation and the tissue change of sperm cavity after the interference of the Dmrta2 gene variable spliceosome can be successfully observed.

The application discloses a kind of based on basket culture Scylla paramamosain conservation method, belong to Scylla paramamosain conservation technical field, process includes: the crab seed juvenile crab 1 period mark coarse culture 1 month, select uniform female crab individual size to carry out basket culture, feed in basket living body shellfish, once a week feeds small miscellaneous fish, feeds again, breeds to before reproductive ecdysis;Female crab is mixed with mature male crab in the same pond before reproductive ecdysis, feeds fresh oyster meat, female to male ratio is 1:2, female and male mating again breed 10d, then female crab is hung up in cultivation basket, artificial induced spawning next year, induced spawning salinity 20%~25%.The conservation method of the application promotes mating female crab to develop into paste crab, and the short-term artificial induced spawning of paste crab can reach 25.6%~31.4%, which meets the general commercial seedling raising demand and breeding requirement, solves the problem of poor artificial conservation effect of mud crab breeding, and is suitable for different species source of mud crab original seed protection and commercial use.

The invention provides a method for detecting Antp mRNA expression localization in scylla paramamosain larvae through overall fluorescence in situ hybridization and application, and belongs to the technical field of gene detection. According to the method, the scylla paramamosain larvae stored in paraformaldehyde are treated through methods of rehydration, bleaching, fixation, digestion, pre-hybridization, repeated washing after hybridization and the like, after treatment is finished, the larvae are stored with glycerol, then observation is conducted through a confocal microscope, positioning exploration of the Antp gene in the scylla paramamosain daphnia-shaped larvae is successfully achieved, the experiment operation difficulty is greatly reduced, and the experiment efficiency is improved. According to the method, the traditional fluorescence in-situ hybridization process is simplified, the detection efficiency and accuracy are remarkably improved, meanwhile, the tissue form of the larva is completely reserved, the visual positioning of Antp mRNA in each tissue organ of the scylla paramamosain larva is realized, and an efficient and economical technical scheme is provided for analyzing the development molecular mechanism of the scylla paramamosain larva.

The invention relates to the technical field of intelligent bait casting, and discloses an intelligent bait casting method and system for scylla paramamosain seawater fries, and the method comprises the steps: extracting a motion track and distribution characteristics according to a real-time motion image of the scylla paramamosain fries in a culture pond, and obtaining behavior characteristic parameters of the scylla paramamosain fries; when the activity degree of the motion trail in the behavior characteristic parameters exceeds a historical data threshold value, it is judged that the scylla paramamosain seeds are in a real-time starvation state; according to the linear relation between the historical bait casting amount and the historical hunger state, the real-time bait casting amount is determined; controlling a bait casting device to scatter bait according to the real-time bait casting amount; after the bait distribution is completed, acquiring the feeding image of the offspring seeds again, and analyzing the feeding image of the offspring seeds, so as to obtain feedback data of the distributed bait; based on the feedback data, the linear relation is dynamically adjusted, and the real-time bait casting amount of the bait casting device is updated according to the dynamically-adjusted linear relation; the intelligent bait casting efficiency can be improved.

The application belongs to the technical field of exosomes, and discloses a preparation method of artificial exosomes of blood cells of a crustacean Scylla paramamosain, which mainly comprises the following steps: 1) collecting blood cells of the Scylla paramamosain by centrifugation; 2) collecting cell membranes and cytoplasmic matrix respectively after blood cells are broken and centrifuged; and 3) obtaining artificial exosomes under certain reaction conditions. The exosomes obtained by the production method can be used as a carrier, and by carrying RNA or proteins, can be widely used in immunological research and disease prevention and treatment of the crustacean.