52 results about "Scylla paramamosain" patented technology

The invention discloses a method for detecting the gonad quantity of scylla paramamosain, and particularly relates to a method for quantifying the gonad quantity of scylla paramamosain and a nondestructive testing device for the gonad of scylla paramamosain. The method comprises the following steps: a, selecting artificially bred female scylla paramamosain, and acquiring gonad distribution photos of different scylla paramamosain at gonad development stages by utilizing an infrared imaging technology; b, calculating the ratio of the gonad distribution area to the crab shell area in each photo obtained in the step a; c, weighing the scylla paramamosain selected in the step a, performing anesthesia sampling, then weighing the weight of gonad tissue, and calculating a gonad index (GSI); and d, by taking the gonad index (GSI) in the step c as an x axis and the gonad area ratio in the step b as a y axis, establishing a quantitative linear model of the gonad quantity of the scylla paramamosain, and fitting a curve function as y = 1.1835 x + 32.646. The technical problem of'accurate detection and quantification of the sexual gland of the scylla paramamosain 'is solved, and the method has practical significance in the aspects of quality selection of the scylla paramamosain and screening of breeding crabs and butter crabs.

The invention relates to a sex-specific genome sequence of scylla paramamosain and a genetic sex identification method. The sex-specific genome sequence is as shown in SEQ NO.1 and SEQ NO.2; wherein the genomes of the individuals with the genetic sex being female contain the sequences SEQ NO.1 and SEQ NO.2 at the same time, and the genomes of the individuals with the genetic sex being male only contain the sequences SEQ NO.2. Meanwhile, the invention also develops a genetic sex identification method based on a PCR (Polymerase Chain Reaction) technology by utilizing the sex specific sequence. The method can be used for identification of sex specific large fragments of scylla paramamosain and rapid detection of genetic sex, has the advantages of high specificity, high accuracy, simplicity and convenience in operation, low cost and the like, can be used in the fields of scylla paramamosain sex control breeding industry, sex determination and differentiation mechanism research and the like, and has a wide application prospect.

The utility model belongs to the technical field of aquaculture, and particularly relates to a production device for in-vitro cultivation of scylla paramamosain embryos, which comprises a conical barrel, a rubber ring, a screen, a handle, a cylindrical base, a drain pipe and an oxygenating device, a drain hole is arranged at the bottom of the conical barrel provided with the handle, and the bottom of the drain hole is connected with a PVC (polyvinyl chloride) water pipe. A row of aquaculture water switches are arranged at the tail end of the PVC water pipe; two rubber ring screens with different diameters, which are made of rubber rings and screens, are embedded at different positions of the conical barrel, the small rubber ring screen at the lower part can prevent the in-vitro embryo of the scylla paramamosain from sinking at the bottom of the conical barrel, and the large rubber ring screen at the upper part can prevent the embryo from being taken out of the water surface to be adhered to the barrel wall of the conical barrel during inflation; meanwhile, in-vitro embryos can be prevented from being discharged by siphoning when water is changed; oxygen needed by the embryos in the cultivation process can be guaranteed through air pipe holes located in the small rubber ring screen and the bottom drainage hole, and the embryos are suspended in the cultivation water by adjusting an air valve switch.

The invention relates to the technical field of ecological breeding of scylla paramamosain and protection and restoration of mangrove forests. The invention provides a method for culturing scylla paramamosain by using mangrove forest, which comprises the following steps: (1) carrying out simple engineering transformation on the basis of a coastal wetland traditional culture pond, constructing a planting island in the middle of the culture pond, and reserving an annular tidal creek between the planting island and the periphery of the culture pond; (2) planting mangrove plants in the planting island to form a man-made forest; (3) small aquatic plants are planted at the bottom of the artificial forest, and large algae are planted in the annular ditches; and (4) putting the young blue crabs into the culture pond, and carrying out daily management until the blue crabs are harvested. The shellfish is used for providing main food supply for the blue crabs, and meanwhile water is purified; excrement of the blue crabs can be fed back to promote growth of mangroves, algae, mussels and the like; large algae can perform photosynthesis at the bottom of the pond to increase dissolved oxygen in water, and small sea grass is placed in the planting island to make up for the defect of low coverage of newly planted mangrove forest plant seedlings.

The application discloses a SNP molecular marker related to the thickness of a left chelae of Scylla paramamosain, a detection primer pair, a kit and purposes. The SNP molecular marker is that the 184th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the left chelae thickness of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The invention discloses an SNP molecular marker related to body weight and / or body height of scylla paramamosain, a detection primer pair, a kit and application. According to the SNP molecular marker, the 111 basic group from the 5'terminal of a nucleotide sequence shown in SEQ ID NO: 1 is C or T. The SNP molecular marker can predict the body weight and / or body height character of an individual in the early breeding stage and is used for molecular marker assisted selective breeding (MAS), the breeding efficiency and accuracy of scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The present application relates to a kind of Scylla paramamosain gender-specific genomic sequences and genetic gender identification method, the gender-specific genomic sequence is as SEQ NO.1 and as SEQ NO.2 shown in sequence;Wherein, genetic gender is female individual genome simultaneously containing SEQ NO.1 and SEQ NO.2 sequence, genetic gender is male individual genome only containing SEQ NO.2 sequence.Simultaneously, the present application also uses the gender-specific sequence to develop the genetic gender identification method based on PCR technology.The present application can be used for the identification of Scylla paramamosain gender-specific large fragment and genetic gender rapid detection, with the advantages of strong specificity, high accuracy, simple operation, low cost etc., can be used for crab sex control breeding industry and gender determination and differentiation mechanism research etc., with wide application prospect.

A non-injury detection method for the ovary development stage of Scylla paramamosain, comprising the following steps: obtaining a fitting curve equation with the ovary development stage number as the variable and the cross-sectional area ratio of ovary to carapace as the dependent variable; screening individuals to be detected that meet the conditions; performing anesthesia pretreatment; placing the individual to be detected under a light source to irradiate it so that the shadow profile of the ovary appears on the carapace; obtaining an image including the carapace under the irradiation of the light source; obtaining the area value of the shadow profile of the ovary on the carapace and the image area value of the carapace in the image respectively, and calculating the ratio of the two; substituting the ratio as the dependent variable into the fitting curve equation to obtain the variable corresponding to the dependent variable, and rounding the variable to an integer to obtain the ovary development stage number of the female Scylla paramamosain individual. The present application can avoid causing body damage to the detected Scylla paramamosain, and ensure that the detected Scylla paramamosain continues to be used for production and sales and fry breeding.

The invention relates to the technical field of aquaculture feed, in particular to a feed additive for improving ovary color of scylla paramamosain and application of the feed additive. The feed additive is prepared from the following raw material components in parts by mass: 30-60 parts of Chinese wolfberry fruit powder, 10-30 parts of a marigold extract, 2-8 parts of chenodeoxycholic acid and 5-15 parts of vitamin E powder. The additive systematically solves the technical bottlenecks of low absorptivity and unstable deposition of natural pigments in crab bodies through the synergistic effect of all the components. When the feed additive is added into feed for fattening female scylla paramamosain, the ovary redness value can be remarkably increased, the color is rich and natural, and meanwhile, the growth of parent crabs and the development of gonad are promoted. The feed is completely prepared from natural raw materials, is safe, free of residues and controllable in cost, and provides an effective scheme for realizing high-valued and green cultivation of scylla paramamosain'red cream crab '.

The invention discloses a counting device for large-size fries of scylla paramamosain and a using method of the counting device, and relates to the technical field of aquaculture equipment. The counting equipment adopts a modular design and comprises a conveying system, a counting system, a juvenile crab throwing box and a juvenile crab box, a tail baffle of the juvenile crab throwing box can move back and forth according to the number of juvenile crabs; a driving motor of the conveying system is arranged at one end far away from the juvenile crab throwing box to prevent water dripped and splashed from the juvenile crab device from damaging the driving motor, the conveying system is embedded with a double-leaf door of the juvenile crab throwing box, and juvenile crabs in the juvenile crab throwing box are conveyed one by one through a conveying belt, pass through the counting system and enter the juvenile crab box after counting; counting equipment adopts an infrared digital display system and is fixed on a supporting plate on one side, close to a driving motor, of the conveying system, the juvenile crabs on a conveying belt are counted after passing through a scanning recognition area, a height limiting baffle with adjustable height is arranged at a double-leaf door, the juvenile crabs can pass through the counting system one by one, and the counting accuracy is improved.

The application discloses a scylla paramamosain antibacterial polypeptide Spgilstin 10‑33 and application thereof, a molecular formula of which is C 118 H 206 N 42 O 30 S, and an amino acid sequence of the Spgilstin is shown as SEQ ID NO. 01. The application has broad-spectrum antibacterial and antimycotic activities, high bactericidal efficiency, no cytotoxicity, high safety, and can be applied to antibacterial agents, antimold agents and aquatic feed additives as effective components, and has wide application prospects.

The invention discloses a specific SNP molecular marker for sex identification of scylla paramamosain as well as a detection reagent and application of the specific SNP molecular marker, the SNP molecular marker is located at the 601st site of a nucleotide sequence SEQ ID NO: 1, and the sex of scylla paramamosain can be simply, rapidly, effectively and accurately identified according to the SNP molecular marker. The invention further provides a detection reagent of the SNP molecular marker, and the detection reagent can be applied to early auxiliary screening of scylla paramamosain, so that the breeding time of scylla paramamosain is shortened, and the breeding process is accelerated.

This invention belongs to the field of biotechnology, specifically relating to a non-harmful DNA extraction method for *Scylla serrata* (scuttica crab) and its application. This invention successfully achieves non-harmful DNA extraction from *Scylla serrata* by collecting its swimming leg setae, significantly reducing the impact on the crab's activity and ensuring its survival after sampling. The extraction method of this invention is non-lethal to *Scylla serrata* and possesses good repeatability and high accuracy. Extracting DNA from *Scylla serrata* using its swimming leg setae ensures that the crab's activity remains unchanged, allowing it to survive and perform its life activities normally. Obtaining the DNA sequence of *Scylla serrata* and performing gene markers provides a foundation for genomic selection breeding, facilitating parent selection and offspring breeding in mud crab genetics.

The invention discloses a scylla paramamosain SNP (Single Nucleotide Polymorphism) molecular marker related to a third-right-step foot length and a section length, a detection primer pair, a kit and application. The SNP molecular marker is characterized in that the 108th basic group from the 5'end of a nucleotide sequence shown as SEQ ID NO: 1 is C or A. The SNP molecular marker can predict the third right step foot length and section length character of an individual in the early breeding stage and is used for molecular marker-assisted selective breeding, the breeding efficiency and accuracy of scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefit is improved.

The invention discloses a scylla paramamosain carapace length related SNP (Single Nucleotide Polymorphism) molecular marker, a detection primer pair, a kit and application. According to the SNP molecular marker, the 179th basic group from the 5'end of a nucleotide sequence shown in SEQ ID NO: 1 is G or T. The SNP molecular marker can predict the carapace length character of an individual in the early breeding stage, and is used for molecular marker-assisted selective breeding, improving the breeding efficiency and accuracy of scylla paramamosain, shortening the breeding period, reducing the breeding cost and improving the economic benefit.

The present invention provides a method for promoting the re-incubation of blue crab brood crabs, and belongs to the technical field of aquaculture. The method for promoting the re-incubation of blue crab brood crabs provided by the present invention feeds fresh razor clams and clams, and at the same time, performs pond transfer treatment on the roe crabs to promote production, so that the number of brood crabs and the number of eggs laid are increased. It can be seen that the method provided by the present invention can not only effectively improve the utilization rate of blue crab brood crabs, alleviate the problem of insufficient blue crab brood crabs during the seedling raising period, but also effectively ensure the number of blue crab seedlings, which is conducive to the sustainable and healthy development of the blue crab breeding industry.

The invention belongs to the technical field of scylla paramamosain aquaculture, and particularly relates to a multi-channel feeding inspection robot for scylla paramamosain industrial aquaculture, which comprises an AGV (Automatic Guided Vehicle) and a main body bracket, the bottom end of the main body bracket is fixedly mounted at the top end of the AGV trolley; a central host is mounted on the main body bracket; a plurality of groups of regulation and control hosts are mounted on the side wall of the main body bracket; a plurality of groups of XYZ linear guide rail modules are mounted on the main body bracket, and the regulation and control host controls the XYZ linear guide rail modules corresponding to the XYZ linear guide rail modules in position; the central host controls the multiple groups of regulation and control hosts and the AGVs; the XYZ linear rail module is provided with a stock bin; a stock bin cover is hinged to the top end of the stock bin; a discharging mechanism is arranged in the stock bin; the invention provides a multi-channel feeding inspection robot for industrial aquaculture of scylla paramamosain. The multi-channel feeding inspection robot is used for solving the problem of low feeding efficiency in the prior art.

The invention discloses a lossless intelligent staging method and system for oocytes of scylla paramamosain. The lossless intelligent staging method comprises the following steps: obtaining morphological parameters of living scylla paramamosain at least including the first length and the body weight; the morphological parameters are input into an XGBoost-based feature enhancement regression prediction model which is subjected to Pipeline packaging training in advance and has optimal parameters, and an oocyte diameter prediction value is obtained; accurately matching a preset staging standard according to the predicted value, and determining an ovarian development staging; and when the prediction confidence coefficient is low, triggering identification and carrying out incremental learning on the model by utilizing confirmation data so as to adaptively optimize the model precision. According to the method, machine learning is used for replacing anatomy to realize nondestructive measurement of the oocyte diameter of the blue crab, and a diameter direct connection ovary development stage table automatic matching development stage is predicted. The simplest configuration of the method only needs the measurement of the nail length and the body weight as effective parameters, the lightweight model can be applied to a quick online lossless ovarian development staging system of the blue crabs, and the intelligent staging can effectively guide the actual breeding decision.

The invention provides a coding gene of scylla paramamosain allergen troponin C and application of the coding gene. The amino acid sequence of the allergen is shown as SEQ ID NO: 1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO: 2. The method comprises the following steps: constructing a recombinant expression plasmid of a scylla paramamosain troponin C (TnC) coding gene, transferring the recombinant expression plasmid into a host cell, inducing expression, and purifying to obtain the recombinant scylla paramamosain sensitization protein TnC. The TnC can be combined with IgE in the serum of a crustacean allergy patient, the expression of CD63 and CD203c on the basophilic granulocyte surface in the whole blood of the patient can be remarkably up-regulated, and the sensitizer can be prepared into a diagnostic reagent for sensitizing components in a patient serum sample or a desensitizing preparation, and has a wide application prospect in the fields of diagnosis and treatment of allergic diseases.

This invention provides a method for constructing an efficient ecological algal community for indoor breeding of mud crabs. Based on the growth characteristics of mud crab zoea larvae and their requirements for water quality and nutrition at each stage, a method for constructing a superior microalgal community and a balanced bacterial-algal community in the mud crab breeding pond is proposed. Six novel algal combinations not previously used in mud crab breeding are used to replace native algae, supplemented with probiotics. A daily combination of algae, bacteria, and biological feed for each developmental stage of the larvae (Z1-C1) is designed, significantly improving the survival rate and efficiency of indoor mud crab breeding. Compared to traditional breeding methods that rely entirely on native algae, this artificially constructed algae is more efficient and reproducible, facilitating the standardization of indoor breeding. This invention promotes a refined management model, achieving a balanced bacterial-algal community, stable and controllable water environment, and avoiding the instability caused by complex and variable native algae. This is more conducive to the promotion of mud crab breeding in areas with poor natural water quality.

The invention belongs to the field of aquaculture, and particularly relates to a multi-nutrition-level aquaculture mode suitable for a brackish water pond and application of the multi-nutrition-level aquaculture mode. According to the method, a multi-nutrition-level ecological breeding mode with scylla paramamosain, penaeus vannamei, ostrea rivularis and gracilaria tenuifolia as core breeding objects is constructed, and the variety specification, density, stocking time and water quality regulation and control strategies are standardized; the problems that a brackish water pond culture mode is single, the culture technology falls behind, and the culture surrounding environment is greatly stressed are effectively solved, and the purposes of water area space complementation, bait complementation, high utilization rate, ecological disease prevention and nitrogen and phosphorus cyclic utilization between animals and plants of different nutrition levels and different culture ecological niches are achieved. Results of the embodiment show that by means of the breeding method, the water quality of the pond can be effectively guaranteed, diseases of various economic varieties are reduced, antibiotics are not used in the whole process, and the comprehensive yield and the comprehensive benefits of breeding are improved.

The invention provides a preparation method and application of a scylla paramamosain allergen Scy p1 hypoallergenic derivative specific I gG antibody, a New Zealand white rabbit is subjected to immune treatment by using a Scy p1 hypoallergenic derivative, and antiserum is purified by using a Protei n A Sepharose affinity chromatography column, so that the purity of the antibody is greatly improved, and the obtained I gG antibody is ensured to have high specificity; the purified antibody is subjected to molecular weight detection through SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), and an immunoglobulin heavy chain and an immunoglobulin light chain containing 50kDa and 25kDa are defined, so that the quality of the IgG antibody is verified, and a reliable basis is provided for subsequent application; the obtained specific I gG antibody effectively blocks combination of Scy p 1 and I gE in whole blood of a patient in vitro, effectively improves allergic symptoms of mice in vivo, ensures that the prepared I gG antibody is safer and is expected to be used for immunotherapy, and side effects possibly occurring in the treatment process are reduced.

The application discloses a SNP molecular marker related to the length of the third right step foot of Scylla paramamosain, a detection primer pair, a kit and purposes. The SNP molecular marker is a nucleotide sequence shown in SEQ ID NO:1, wherein the 108th base from the 5' end is C or A. The SNP molecular marker can be used for predicting the length of the third right step foot of an individual at an early breeding stage, and can be used for molecular marker assisted selection breeding, so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.

The application discloses a scylla paramamosain antibacterial peptide Sparamosin 26‑54 The application has significant antiviral effect on white spot syndrome virus, frog iridovirus and eel herpesvirus, has the characteristics of easy synthesis, stability, high antiviral activity and low toxicity, and has good application prospect in drug research.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker combination related to low-salt resistance of scylla paramamosain and application of the SNP molecular marker combination, and the SNP molecular marker combination comprises nine sites SNP1-SNP9 in total. According to the method, combined genotype analysis is carried out based on multiple SNP sites, genetic information of multiple sites is integrated, and the accuracy of predicting the low-salt-tolerant phenotypic character of scylla paramamosain can be remarkably improved. The SNP molecular marker combination is applied to breeding practice of scylla paramamosain, low-salt-resistant performance screening can be performed on parents and individuals in the seedling stage or the early development stage, and a technical basis is provided for cultivating a novel low-salt-resistant scylla paramamosain strain and supporting stable development of the breeding industry.

The invention discloses a scylla paramamosain trunk weight related SNP (Single Nucleotide Polymorphism) molecular marker, a detection primer pair, a kit and application. The SNP molecular marker is characterized in that the 93rd basic group from the 5'terminal of a nucleotide sequence as shown in SEQ ID NO: 1 is C or T. The SNP molecular marker can predict the trunk weight character of an individual in the early breeding stage, is used for molecular marker-assisted selective breeding (MAS), improves the breeding efficiency and accuracy of scylla paramamosain, shortens the breeding period, reduces the breeding cost and improves the economic benefit.

The application discloses a SNP molecular marker related to body weight and / or body height of Scylla paramamosain, a detection primer pair, a kit and application. The SNP molecular marker is that the 111th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1 is C or T. The SNP molecular marker can be used for predicting the body weight and / or body height of an individual in early breeding, and is used for molecular marker assisted selection breeding (MAS), so that the breeding efficiency and precision of the Scylla paramamosain are improved, the breeding period is shortened, the breeding cost is reduced, and the economic benefits are improved.

The invention discloses a reagent for detecting SNP (Single Nucleotide Polymorphism) locus genotypes of salt-tolerant related traits of scylla paramamosain and application of the reagent. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a scylla paramamosain genome. The SNP sites comprise an SNP site 1, an SNP site 2, an SNP site 3 and an SNP site 4; the SNP site 1 is located at the 13938th basic group of the CA gene, the SNP site 2 is located at the 13981th basic group of the CA gene, the SNP site 3 is located at the 18286th basic group of the CA gene, and the SNP site 4 is located at the 18336th basic group of the CA gene. According to the invention, the single nucleotide polymorphism (SNP) of the salt tolerance related characters of the scylla paramamosain character is screened, the SNP is identified through sequencing, the peak map is clear, the typing is reliable, the salt tolerance related characters of the scylla paramamosain character are effectively determined, and valuable information is provided for breeding.

The invention relates to a YOLO-based scylla paramamosain phenotype parameter calculation method, which comprises the following steps of: acquiring multi-angle images of scylla paramamosain to obtain a scylla paramamosain image data set; training a YOLO model which is based on a point sampling method and introduces secondary pooling mechanism fusion through the marked blue crab image data set to obtain a blue crab key point detection model; performing key point prediction on a target blue crab image based on the blue crab key point detection model to obtain position coordinates of key points on blue crab shells; according to the position coordinates of the key points, phenotypic parameters of the blue crabs are calculated, and parameter results are obtained. According to the method, the feature extraction capability of the key points at the edge of the blue crab carapace is enhanced, and the data acquisition and processing efficiency and accuracy of aquaculture are improved.