38 results about "Female individual" patented technology

The application discloses a SNP molecular marker for identifying the genetic sex of Procambarus clarkii, wherein the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No. 1, and a male individual is shown as GG homozygote at the 43th base position of the nucleotide sequence of the SNP molecular marker, and a female individual is shown as GA heterozygote at the 43th base position of the nucleotide sequence of the SNP marker. The application further discloses primers and kits for detecting the SNP molecular marker, and application of the reagents, primers and kits for detecting the SNP molecular marker in genetic sex identification of the Procambarus clarkii. The SNP marker is closely related to the genetic sex, and can be used for conveniently and quickly identifying the genetic sex of the Procambarus clarkii in the process of family breeding and unisexual breeding of the Procambarus clarkii, and provides a theoretical basis for identification of sex-reversed individuals in the breeding process.

The application relates to the technical field of Chinese soft-shelled turtles, in particular to primers, a kit and a method for identifying the genders of Chinese soft-shelled turtles and application. The genomic DNA of male and female individuals of Chinese soft-shelled turtles is respectively subjected to PCR amplification by using the primer pair, and male-specific fragments and female-specific fragments can be obtained. The primer pair, the kit and the method provided in the embodiments can effectively distinguish female individuals and male individuals of Chinese soft-shelled turtles.

The invention discloses a molecular sequence and a primer for sex identification of grassland caterpillars and application of the molecular sequence and the primer. The nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 1 and SEQ ID NO: 2; wherein a nucleotide sequence as shown in SEQ ID NO: 1 is female, and a nucleotide sequence as shown in SEQ ID NO: 2 is male. The invention also provides a primer group for amplifying the molecular fragment, and the nucleotide sequences of the primer group are shown as SEQ ID NO: 3 and SEQ ID NO: 4. Wherein the size of a strip capable of being amplified by a female individual is 484 bp, and the size of a strip capable of being amplified by a male individual is 199 bp. According to the molecular marker and the primer group provided by the invention, sex identification can be carried out on grassland caterpillars, larvae, incomplete pupae and cocoons with unknown sex, the accuracy is high, the speed is high, and a reliable method is provided for monitoring the field sex ratio of the grassland caterpillars.

The present application relates to the field of biotechnology, and more particularly to a mylopharyngodon piceus gender-specific molecular marker, primer and application thereof, wherein the primer designed for the molecular marker is used for PCR amplification, and the genetic sex of the mylopharyngodon piceus can be accurately and rapidly identified. The molecular marker presents stable difference between male and female individuals, and the identification accuracy is 100%. In addition, the molecular marker can be stably applied to mylopharyngodon piceus of different specifications and different breeding strains, and shows good universality and reliability. The present application provides important technical support for the unisexual breeding, germplasm resource management and population structure monitoring of mylopharyngodon piceus, and has wide application prospect.

The application relates to the technical field of Chinese soft-shelled turtles, in particular to primers, a kit and a method for identifying the genders of Chinese soft-shelled turtles and application. The genomic DNA of male and female individuals of Chinese soft-shelled turtles is respectively subjected to PCR amplification by using the primer pair, and male-specific fragments and female-specific fragments can be obtained. The primer pair, the kit and the method provided in the embodiments can effectively distinguish female individuals and male individuals of Chinese soft-shelled turtles.

This invention relates to molecular marker 1769 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 1769 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals who have undergone sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention provides a tussah tussah tall thousand-grain cocoon redirection breeding method which comprises the following steps: partitioning a breeding basic group of tussah, and stocking in a plurality of moth regions under the same ecological condition; after cocooning, measuring the weight of thousand cocoons by taking the moth area as a unit, and screening out a target moth area based on a measurement result; performing sex identification and whole cocoon quantity measurement on the silkworm cocoons in the target moth area, and screening out a target female individual set and a target male individual set according to data of the whole cocoon quantity; regulating and controlling the eclosion time of individuals in the target female individual set and the target male individual set to synchronize the eclosion time, and performing directional mating between the two sets according to a preset pairing rule based on the data of the total cocoon amount; and taking the filial generation obtained by directional mating as a breeding base population of the next generation, and carrying out continuous multi-generation directional breeding until a tussah silkworm strain with stable thousand-grain cocoon weight character is obtained. According to the method, a breeding path of primary screening, fine screening, directional mating and multi-generation closed loop is constructed, so that breakthrough increase of thousand-grain cocoon weight is realized.

This invention relates to the molecular marker M-513 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting a 513bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The present application relates to a kind of Scylla paramamosain gender-specific genomic sequences and genetic gender identification method, the gender-specific genomic sequence is as SEQ NO.1 and as SEQ NO.2 shown in sequence;Wherein, genetic gender is female individual genome simultaneously containing SEQ NO.1 and SEQ NO.2 sequence, genetic gender is male individual genome only containing SEQ NO.2 sequence.Simultaneously, the present application also uses the gender-specific sequence to develop the genetic gender identification method based on PCR technology.The present application can be used for the identification of Scylla paramamosain gender-specific large fragment and genetic gender rapid detection, with the advantages of strong specificity, high accuracy, simple operation, low cost etc., can be used for crab sex control breeding industry and gender determination and differentiation mechanism research etc., with wide application prospect.

This invention relates to the molecular marker M-866 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 866bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention discloses a large fragment deletion-based specific molecular marker for sex of spinibarbus hollandi and application of the specific molecular marker. According to the marker, whole genome re-sequencing data of male and female individuals of barbus hollandi is compared to a reference genome, and a female specific large fragment deletion region located on a No.43 chromosome (chr43) is screened out. Specific primers are designed according to the deletion region, and rapid and accurate identification of the gender of the spinibarbus hollandi can be realized through PCR amplification and electrophoretogram analysis. The method is suitable for sex identification of spinibarbus hollandi offspring seeds in the early development stage, and provides technical support for research on unisexual breeding, sex control and sex determination mechanism.

The present application relates to a kind of sex-related specific DNA fragments of starburst flathead flounder, primer, application and kit, belong to molecular biology field, the nucleotide sequence of the specific DNA fragment is as shown in SEQ ID NO:1, the nucleotide sequence of the primer is as shown in SEQ ID NO:4 and SEQ ID NO:5.The primer is used to carry out PCR amplification to the DNA of starburst flathead flounder, two bands are amplified in male individual, length is 1327bp and 762bp respectively;One band is amplified in female individual, and it is the 762bp fragment.The present application also provides kit comprising the primer for identifying the genetic sex of starburst flathead flounder.The sex-specific molecular marker primer of the present application can accurately and efficiently identify the genetic sex of starburst flathead flounder, and the identification method is simple and easy to operate, and the required cost is low.

The invention discloses an indel marker for identifying sex of larimichthys crocea in different habitats and application thereof, the indel marker is an insertion / deletion nucleotide sequence with the length of 12bp at the 47617128-site basic group of the No.3 chromosome of the larimichthys crocea, the sequence is as shown in SEQ ID NO.1, a female individual has the sequence as shown in SEQ ID NO.1, and a male individual deletes the sequence as shown in SEQ ID NO.1. The invention also discloses a primer and a kit for amplifying the marker, a method for identifying the sex of the large yellow croaker in different habitats by adopting the primer or the kit, and application of the primer, the kit or the method in identifying the sex of the large yellow croaker in different habitats. The method has the characteristics of universality, high resolution, specificity, low cost, easiness in operation and the like, and also has industrialization potential.

The application provides a breeding method of high-quality meat rabbits, and belongs to the technical field of meat rabbit breeding.The breeding method of the application has the following steps: (1) crossbreeding is carried out by taking Fujian yellow rabbits as male parents and Minxi southwest black rabbits as female parents to obtain an HM crossbreeding population; (2) crossbreeding is carried out by taking Sichuan white rabbits as male parents and Fujian yellow rabbits as female parents to obtain a BH crossbreeding population; (3) crossbreeding is carried out by taking male individuals in the BH crossbreeding population as male parents and female individuals in the HM crossbreeding population as female parents to obtain a BHM population; (4) the BHM population is horizontally crossed and fixed to obtain a new strain base population, and the base population is closed and subcultured for four generations, so that a new strain of high-quality meat rabbits is obtained.The high-quality meat rabbits bred by the breeding method of the application have similar size and hair color appearance to wild rabbits, have the characteristics of high meat quality and strong stress resistance, can meet the needs of people for wild rabbit breeding and consumption, and have wide application prospects.

The invention provides a molecular marker and a primer for identifying sex of Nibea dispinosa and application of the molecular marker and the primer, and belongs to fish molecular breeding and sex identification. Whole genome re-sequencing and whole genome association analysis are carried out on male and female individuals of the Nibea dispinosa, male specific DNA fragments are obtained through screening, and corresponding specific primers are designed. The primer is used for carrying out PCR (Polymerase Chain Reaction) amplification on individual genome DNA (Deoxyribose Nucleic Acid), a male individual is amplified to form specific double bands (174bp and 181bp), and a female individual is only amplified to form a single band (174bp), so that the rapid and accurate judgment on the genetic sex of the Nibea dispinosa is realized. The molecular marker and the primer provided by the invention are simple and convenient to operate and small in damage to a fish body, and can be used for early sex identification, sex control breeding and germplasm resource protection of the Nibea dispinosa.

The invention provides application of ergothioneine in improving fertility of high-fertility-age female individuals, and belongs to the technical field of natural medicines. The invention provides an application of EGT in preparation of a product for improving fertility of a high-fertility-age female individual. In the embodiment of the invention, a high-fertility-age ICR female mouse is taken as an animal model, the influence of the EGT on the fertility of the high-fertility-age female mouse is verified from in-vivo and in-vitro aspects, and the result shows that the EGT has the effect of improving the in-vitro fertilization rate and blastocyst rate of oocytes of the high-fertility-age female mouse; the superovulation number and the litter number of high-childbearing-age female mice are increased. It is proved that the EGT has the effect of improving the fertility of high-fertility-age female individuals.

The invention provides an ictalurus punctatus dnmt1 gene knockout RNAi target sequence, a sex regulation and control method and application, and belongs to the technical field of sex regulation and control. An RNAi sequence is designed based on the dnmt1 gene, and gonad differentiation intervention independent of exogenous hormones is realized in the species by inhibiting expression of the dnmt1 gene in the channel catfish. Experimental results show that dnmt1 expression down-regulation can effectively induce partial sex reversal of genetic female individuals, typical testis-like structures appear in ovarian tissues of the female individuals, it is proved that dnmt1 plays a key role in maintaining female gonad development stability, and it is hopeful that dnmt1 regulation is combined with a genetic screening or gene editing technology, so that the development stability of the female gonad is improved. A stable and efficient all-male channel catfish breeding system is established, and a new path is developed for all-male offspring seed production.

The present application belongs to the technical field of molecular biology, and particularly relates to a gender identification molecular marker based on Y chromosome specific deletion of Micropterus salmoides, a primer pair and a gender identification method. The marker disclosed by the present application is a 147 bp deletion sequence (SEQ ID NO. 1) on the Y chromosome of male individuals, and has a complete genetic relationship with gender, wherein the XX genotype corresponds to female individuals, the XY genotype corresponds to male individuals, and the YY genotype corresponds to super-male individuals. Meanwhile, a primer pair for specifically amplifying the marker is provided. When detection is performed, only the genomic DNA of fin tissue of an individual to be detected needs to be extracted, PCR amplification is performed by using the primer pair, and the amplified bands are analyzed by agarose gel electrophoresis. Then, the genotype can be accurately determined according to the number of bands. The method has the remarkable advantages of simple operation, low cost and reliable results.

The invention belongs to the technical field of livestock breeding, and particularly relates to a rapid breeding method for a new variety of medium-sized golden Luhua commercial chickens. The breeding method comprises the following steps: hybridizing a first female parent and a first male parent to obtain an F1-generation group; selecting F1-generation female individuals as backcross female parents, and carrying out grading hybridization on the backcross female parents and the first male parents or chickens of the same species to obtain F2-generation groups; and selecting the F2-generation male individual as a terminal male parent, and carrying out three-way hybridization on the terminal male parent and the second female parent to obtain commercial-generation chickens. According to the breeding method, through the combination of grading hybridization and three-way hybridization, the excellent quality characters are consolidated firstly, then the three-way hybridization is used for stimulating the heterosis, the bred commercial chickens have the advantages of being high in growth speed, moderate in body type, delicious in meat quality and the like, and the breeding method is clear in breeding path and high in commercial value. In addition, the breeding method effectively solves the technical problem that the existing chicken breeds are difficult to consider a plurality of excellent characters such as excellent meat quality and rapid growth.

The invention discloses a molecular marker primer pair for identifying sex of siniperca chuatsi, a kit, application and a method. On the basis of high-throughput sequencing and comparative genomics analysis, a specific DNA fragment which stably exists in a male individual of siniperca chuatsi and is deleted in a female individual is found for the first time, and a specific molecular marker (as shown in SEQ ID NO: 1) and a primer pair are developed on the basis of the specific DNA fragment. The marker is used for PCR amplification, the genetic sex of mandarin fish can be quickly, accurately and noninvasively identified only through conventional agarose gel electrophoresis, and the accuracy rate reaches 95% or above. The invention also provides a kit containing the primer pair, an identification method and application. The method has the advantages of wide population application, stable and reliable result, convenience in operation under conventional laboratory conditions and the like, and is suitable for sex identification or breeding of the siniperca chuatsi, such as early identification of genetic sex of the siniperca chuatsi, screening of specific breeding materials and the like.

The application discloses a primer pair, a kit, a method for identifying the gender of Chinese soft-shelled turtles and application of the method. The primer pair comprises an upstream primer shown in SEQ ID NO: 2 and a downstream primer shown in SEQ ID NO: 3. The inventors of the application find a female-specific fragment SEQ ID NO: 1 by performing genome sequencing on male and female Chinese soft-shelled turtles, and design the above primer pair based on the fragment. The primer pair is used to perform PCR amplification on the genomic DNA of Chinese soft-shelled turtles as a template, and a 548 bp specific band containing the sequence shown in SEQ ID NO: 1 can be amplified from female individuals, and no band can be amplified from male individuals. The application further discloses a kit containing the primer pair, a method for identifying the gender of Chinese soft-shelled turtles and application of the primer pair, the kit or the method in gender identification and breeding of Chinese soft-shelled turtles. The application provides a new molecular marker selection for gender identification of Chinese soft-shelled turtles, and is simple in operation, rapid in detection and clear and reliable in result.

The invention belongs to the technical field of molecular biology, and particularly relates to a sex identification molecular marker based on micropterus salmoides Y chromosome specific deletion, a primer pair and a sex identification method. The marker disclosed by the invention is a 147bp deletion sequence (SEQ ID NO.1) on a Y chromosome of a male individual, and is in a complete linkage genetic relationship with sex, an XX genotype corresponds to a female individual, an XY genotype corresponds to a male individual, a YY genotype corresponds to a super-male individual, meanwhile, a primer pair for specifically amplifying the marker is provided, and the primer pair is used for detecting sex of the male individual. During detection, the genotype can be accurately judged according to the number of bands only by extracting the genome DNA of the fin ray tissue of an individual to be detected, performing PCR amplification by using the primer pair and analyzing amplified bands through agarose gel electrophoresis, and the method has the remarkable advantages of simplicity and convenience in operation, low cost and reliable result.

The application relates to a molecular marker M-428 related to identification of sex-reversed pseudo-male individuals of Rana dybowskii and application thereof, and relates to the field of molecular markers. In order to solve the problem that sex-reversed pseudo-male individuals of Rana dybowskii are difficult to identify at present, the application extracts the genome of the left hind limb muscle tissue of Rana dybowskii, takes the extracted genomic DNA as a template, and carries out a PCR amplification reaction with a primer pair containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification product is subjected to electrophoresis detection through 3% agarose gel, the amplification result has a 428bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3, the phenotype male individual is a genetic male Rana dybowskii, i.e. a true male individual. The phenotype male individual missing the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 is a pseudo-male individual with sex reversal from a genetic female to a phenotype male. The phenotype female individual missing the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 is a female individual.

The present invention relates to a composition comprising one or more fatty acid derivatives for use in promoting, supporting or optimizing one or more of the following: (i) de novo myelination; (ii) brain structure; (iii) brain connection; (iv) intellectual potential; (v) cognitive potential; and (vi) learning potential; (vii) cognitive function, wherein the composition is for administration to the female subject.

The invention discloses a molecular marker, a primer group and a kit for identifying gender of pelochelys bibroni and application of the molecular marker, the primer group and the kit. The molecular marker has a nucleotide sequence as shown in SEQ ID NO: 1, stably exists in a pelochelys bibroni female individual, and is deleted in a male individual. On the basis, the invention designs a specific primer group which can be used for detecting the molecular marker through PCR (Polymerase Chain Reaction) amplification. The invention also provides a kit containing the primer group and a non-diagnostic identification method. According to the method, the sex is judged by detecting whether a PCR amplification product exists or not: the sex is female when a specific band appears, and the sex is male when no band exists. The specific molecular target for pelochelys bibroni sex identification is provided for the first time, the problem that early-stage and non-invasive sex identification cannot be conducted in the prior art is solved, and the method has the advantages of being accurate, rapid and easy and convenient to operate and has great significance on artificial breeding and population protection of pelochelys bibroni.

This invention discloses an indel marker for identifying the sex of large yellow croaker from different habitats and its application. The indel marker is a 12 bp insertion / deletion nucleotide sequence located at position 47617128 of chromosome 3 of the large yellow croaker, as shown in SEQ ID NO.1. Female individuals have the sequence shown in SEQ ID NO.1, while male individuals lack the sequence shown in SEQ ID NO.1. The invention also discloses primers and kits for amplifying the marker, a method for identifying the sex of large yellow croaker from different habitats using the primers or kits, and the application of the aforementioned primers, kits, or methods in identifying the sex of large yellow croaker from different habitats. This invention features universality, high resolution and specificity, low cost and ease of operation, and also has industrialization potential.

A method for managing miscarriage in a female individual suffering from a pregnancy loss, comprising at least: a) obtaining a set of data comprising pregnancy loss data associated with a foetus and / or foetal tissue, said pregnancy loss data comprising at least a βhCG value (IU / L) in said female individual, measured at the time of the determination of the pregnancy loss, b) determining one or more management score based on said set of data comprising at least a first management score predictive of a most suitable type of management of miscarriage to be received by said female individual at the time of the pregnancy loss, for removing the foetus and / or foetal tissue, c) predicting the most suitable type of management of miscarriage to be received by said female individual based on said one or more management score comprising said first management score, and d) removing said foetus and / or foetal material according to said most suitable type of management of miscarriage predicted based on said one or more management score comprising said first management score; wherein said most suitable type of management of miscarriage predicted based on said one or more management score comprising said first management score increases the most the likelihood of occurrence of an ovulation during a period of 6 weeks after said pregnancy loss in said female individual.

The invention discloses a sex identification method for seriola aureovittata, which comprises the following steps: (1) designing a primer for amplifying a DNA (Deoxyribonucleic Acid) fragment according to the sex-related DNA fragment of the seriola aureovittata; (2) extracting DNA (deoxyribonucleic acid) of a seriola quinqueradiata individual to be subjected to sex identification, performing PCR (polymerase chain reaction) by taking the DNA as a template and adopting the primer in the step (1), obtaining a PCR amplification product of the seriola quinqueradiata individual to be subjected to sex identification after the reaction is finished, performing enzyme digestion on the PCR amplification product, and performing electrophoresis identification on the product after the enzyme digestion; according to the invention, one mononucleotide mutation site GAT (T / C) ATC between the sexual seriola quinqueradiata male and female individuals is utilized to construct an enzyme digestion map capable of correctly distinguishing the sexual seriola quinqueradiata male and female individuals, so that the PCR-RFLP method for accurately and rapidly identifying sexual seriola quinqueradiata is established.

A method for predicting the likelihood of occurrence of an ovulation during a period of 6 weeks after the detection of a pregnancy loss in a female individual, said method comprising at least: a) obtaining a set of data comprising pregnancy loss data associated with a foetus and / or foetal tissue, said pregnancy loss data comprising at least a βhCG value (IU / L) in said female individual, measured at the time of the determination of the pregnancy loss, b) determining one or more likelihood score based on said set of data, c) predicting the likelihood of the occurrence of an ovulation in said female individual during a period of 6 weeks after a pregnancy loss based on said one or more likelihood score.

This invention relates to molecular marker 897 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 897 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.