34 results about "Complete linkage" patented technology

An object of the present invention to provide a hybrid plant having a high fertility and a method for producing such a hybrid plant. The hybrid plant of the present invention is characterized by having two or more copies of a fertility restorer gene at two or more gene loci which do not have a complete linkage relationship. Further, the method of the present invention comprises introducing a fertility restorer gene by genetic engineering and placing two or more copies of a fertility restorer gene at two or more gene loci which do not have a complete linkage relationship.

The automatically centering central frame consists of support, left large sector gear, left small sector gear, left pressure lever, right pressure lever, roller, upper rotary pin, right small sector gear, right large sector gear, link rod, sliding block, runner block, lower pressure lever slide rail, lower pressure lever and lower rotary pin. It is one opened structure and realizes three bearing point automatic centering by means of the block-link rod mechanism to drive the sector gear mechanism. The complete linkage of the three bearing points can center shaft work piece of different diameter reliably in the same rotary center. The present invention has the features of reliable design, simple structure, wide application range, etc and is especially suitable for automatic control of central frame by means of pneumatic, hydraulic or other modes.

The invention relates to a molecular mark of a high lysine gene in Zea mays L., and applications of the same. The molecular mark is InDel molecular mark-4.9 and 5.7 which both can be amplified through a PCR reaction and detected through an agarose gel electrophoresis, see base sequences represented by SEQ ID No: 1. By using the molecular mark in complete linkage with 5512G gene, genotype identification can be carried out on DNA of any organization of Zea mays L. in any period, and it is detected whether the 5512G in each individual plant of cross breeding filial generation exists or not in a heterozygosis or homozygous state Simultaneously, by using the molecular mark, the mutant and 22 domestic mainly-breeding parents can be distinguished, and the existence of 5512G can be detected accurately in any breeding background populations. The detection method is high in accuracy and simple in operation, and provides an important technology means for breeding assisted by DNA molecular mark of 5512G.

The invention discloses a map linkage method and relates to the electronic field. The method includes the following steps: S1, constructing a map of the control layer, and the full coverage of the map of the control layer needs to be linked with a map of the display layer; S2, selecting any map from the maps of the display layer that need to be linked as the main map of the display layer, The map of the control layer is bound to the map of the main display layer; S3, the map of the control layer obtains the user's operation instruction through the map of the main display layer, and then sends the order in real time to the map of the sub-display layer except the map of the main display layer Directive operation control map instruction; S4, the sub-display layer map adjusts the map according to the one-way operation control map instruction, and completes the linkage of all maps that need to be displayed. The present invention ensures that all the maps on the presentation layer can simultaneously receive the command for changing the display range of the map, thereby ensuring the absolute synchronization effect of all the maps during linkage.

The invention discloses an SNP (single-nucleotide polymorphism) marker correlated with detection of tail type characters of sheep and an application of the SNP marker. The SNP marker correlated with detection of the tail type characters of the sheep in China is characterized by being located in two loci, namely, 87804590 / 87804589 of chromosome 8 of the sheep; the two loci of the SNP molecular marker are linked completely; polymorphisms are G-G and C-T; the genotypes comprise G-G / G-G, G-G / C-T and C-T / C-T, the genotype C-T / C-T is only detected in fat-rumped sheep in China, and statistical testsprove that the genotype C-T / C-T is significantly correlated with the tailless phenotype of the sheep. The SNP marker can be applied to early breeding for the tail type characters of local sheep breedsin China, accurate screening can be even performed on sheep in embryonic period or at birth, and a method has the advantages of being accurate, reliable and simple and convenient to operate when usedfor identifying local purebred fat-rumped sheep breeds in China.

The invention relates to a molecular marking method for two mutation sites in a chicken PTHLH gene 5' regulatory region and application thereof in chicken breeding. Two mutation sites (namely the -1827 (A>G) site and the -165(C>T) site) exist in the chicken PTHLH gene 5' regulatory region, SHEsis online software is used for linkage disequilibrium analysis, and the result shows that the two sites in recessive white plymouth rock are in a complete linkage state. The two sites are related with the first-egg age and egg laying quantity at the 32th week, the first-egg age of AC / GT gene type is remarkably earlier than that of the GT / GT type and the AC / AC type, and meanwhile the egg laying quantity at the 32th week is remarkably larger than that of the other two types. The method is simple, fast and beneficial for breeding chicken variety with the early first-egg age and the large egg laying quantity and provides favorable help for marker-assisted breeding work.

The invention discloses a fire linkage control method and system, wherein the method and the system are applied to urban rail transit. The system uses environment equipment and a monitoring system as a platform to carry out interface relevance with various specialties. According to actual situations, a comprehensive linkage scheme is developed and is operated by a user. The coordination linkage ability of fire disaster relief can be greatly improved. The efficiency of disaster relief is improved. According to the invention, through a redundancy mechanism, the reliability and the stability of a fire disaster relief system are enhanced; when an overall system scheme is designed, an optimized fire alarm confirmation technology is used; the method of combined and mutually redundant FAS and BAS is used for linkage; a unique fire mode instruction transmission and receiving technology, an advanced fire mode instruction decomposition technology and a fire mode linkage control drive technology are used; processing programs carry out communication for various kinds of different professional equipment; and various kinds of data are processed to develop the complete linkage scheme for the user to choose.

The invention belongs to the field of rape breeding, particularly relates to rape self-incompatibility complete linkage dominant SCAR labels and the application thereof. Cabbage type rape self-incompatibility S locus dominant SCAR molecular labels are produced artificially, which includes self-incompatibility S locus dominant labels and self-incompatible restorer line S locus dominant labels, wherein the self-incompatibility S locus dominant SCAR labels are named as SRKa, SEKb and SP11a, whose nucleotide sequences are displayed in sequence tables with SEQ ID NO: 1, 2, 6; the self-incompatiblerestorer line S locus dominant SCAR labels are named as SLGa, SLGb and SLGc, whose nucleotide sequences are displayed in sequence tables with SEQ ID NO: 3, 4, 5. The molecular labels can differentiate homozygous-compatible, hybrid-compatible and homozygous-incompatible genes. The molecular labels are used to verify the cabbage type rape self-incompatibility phenotype. The invention provides practical labels and a usage method for rape molecular breeding.

The invention belongs to the technical field of molecular genetics, and in particular relates to a group of SNP molecular markers used for screening and / or detecting the abnormality rate of sperm of breeding bulls. The PRKCB gene has 12 fully linked SNP sites, and 2 haplotypes were obtained by haplotype analysis, namely H1 (TCTGTTTCTAAC) and H2 (CTCACCCTCTCA), and there are a total of 3 haplotype combinations H1H1, H1H2, and H2H2. The correlation analysis with semen quality of bulls showed that the deformity rate of bulls with H1H2 haplotype combination was significantly lower than that of bulls with H1H1 and H2H2 haplotype combination after thawing frozen semen (P<0.05).

The invention discloses a method for detecting polymorphism of multiple single nucleotides in a 5' control region of a cattle CIDEC gene. The method comprises the following steps of: with a to-be-detected cattle complete genome DAN containing a CIDEC gene as a template and primer pairs P (P1, P2 and P3) as primers, performing amplification on the cattle CIDEC gene; respectively digesting PCR amplification products with restriction enzymes such as HaeIII, AccI and HaeIII, then carrying out agarose gel electrophoresis on a digested amplified fragment, and identifying polymorphism of single nucleotides at a 974th site, a 956th site and a 501st site of the CIDEC gene of the cattle according to results of agarose gel electrophoresis, wherein polymorphism of the single nucleotide at the 956th site is also the polymorphism of single nucleotides at a 841st site, a 763rd site, 727th site and a 546th site, the polymorphism of the single nucleotide at the 501st site is also the polymorphism of a single nucleotide at a 643rd site owning to complete linkage, and thus polymorphism information of nine sites can be obtained by detecting polymorphism of three sites. The detection method provided by the invention lays a foundation for establishing a relation between the polymorphism of single nucleotides of the CIDEC gene and growth traits, so as to be convenient for marker assisted selection (MAS) on the growth traits for cattle beef in China, and a cattle population with excellent genetic resources can be quickly established.