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MC4R gene molecular marker related to Sujiang pig production traits as well as preparation method and application thereof

A technology for molecular markers and production traits, which can be used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as little effect

Active Publication Date: 2020-11-06
JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding methods have had little effect on low heritability traits such as production traits

Method used

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  • MC4R gene molecular marker related to Sujiang pig production traits as well as preparation method and application thereof
  • MC4R gene molecular marker related to Sujiang pig production traits as well as preparation method and application thereof
  • MC4R gene molecular marker related to Sujiang pig production traits as well as preparation method and application thereof

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Embodiment Construction

[0024] Below in conjunction with specific embodiment the present invention is described in further detail. Given detailed implementation and specific operation process, examples will help to understand the present invention, but the protection scope of the present invention is not limited to the following examples.

[0025] 1. Identification of MC4R Gene Genetic Variation Sites in Sujiang Pigs

[0026] (1) Reference sequence download and primer design

[0027] From Ensembl ASIA Download the porcine reference genome version 11.1 MC4R gene (ENSSSCG00000051798) sequence SEQ ID NO: 1 ( http: / / asia.ensembl.org / index.html ), including exons, introns, and 600bp 5' flanking sequences and 3' flanking sequences, PCR primers were designed using Primer 5.0 for the exons (as shown in Table 1).

[0028] Table 1 Exon PCR primers

[0029]

[0030] (2) PCR amplification

[0031] Randomly select 100 Sujiang pig DNA samples, uniformly dilute to 50 ng / μL, take 10 μL from each sample, and...

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Abstract

The invention discloses an MC4R gene molecular marker related to Sujiang pig production traits. The locus of the molecular marker is selected from at least one of the following loci: (1) locus mutation from C to T of a 160773885th basic group (rs335628164) of a No.1 chromosome of a 11.1 version of a pig reference genome, and (2) locus mutation from A to T of the 160773971st basic group (rs81221063) of the No.1 chromosome of the 11.1 version of the pig reference genome, wherein the two loci are completely interlocked. The correlation analysis determines that the two molecular markers are significantly related to the production traits of the Sujiang pigs, and can be used for molecular marker-assisted selective breeding of the Sujiang pigs.

Description

technical field [0001] The invention belongs to the field of pig marker-assisted selection (MAS) technology and genetic breeding, and relates to the preparation and application of molecular markers that can be used for marker-assisted selection of production traits of Sujiang pigs. Background technique [0002] Affected by African swine fever, the domestic pig stock is insufficient, which seriously affects the supply of pork, especially the supply of high-quality pork. High-quality breeding pigs are the guarantee of high-quality pork. Traditional breeding methods have had little success for low heritability traits such as production traits. With the development of molecular biology techniques, the efficiency and accuracy of pig breeding selection can be improved by molecular marker-assisted selection (MAS) for traits with low heritability, phenotypic values ​​that are difficult to measure or costly to measure. [0003] Melanocortin 4 receptors (MC4R) is a carrier for trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 刘林雨徐盼马政仲德赵旭庭周春宝陶勇倪黎纲
Owner JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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