37 results about "R gene" patented technology

Compositions and methods for enhancing the resistance of wheat and barley plants to wheat stripe rust caused by Puccinia striiformis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of wheat and barley plants to wheat stripe rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat or barley plant cell. Additionally provided are methods for using the wheat and barley plants in agriculture to limit wheat stripe rust.

The present invention relates to a method for down regulating an Arabidopsis protein phosphatase type 2C gene, referred to as “defense-associated protein phosphatase type 2C one” (DAPP1) that functions as a negative regulator of a plant defense pathway by contacting the gene or gene mRNA with an interfering nucleotide sequence that interacts with the gene and reducing expression thereof. Plants including such interfering nucleotide sequence exhibit increased disease resistance to pathogen even in the absence of R genes. Close homologs of DAPP1 exist in multiple crop species, and as such, the controlled down-regulation of homologous genes in a variety of crop species will enhance disease resistance of target crop species to pathogens.

The invention belongs to the technical field of biology, and relates to application of a new gene, in particular to an R gene for controlling soybean-rhizobium matching property as well as protein and application of the R gene. The GmNNL1 genome sequence of the gene GmNNL1 disclosed by the invention in the HENGFENG WUDOU of a soybean line is as shown in SEQ ID NO. 2 and the coded amino acid sequence is as shown in SEQ ID NO. 3. The R gene GmNNL1 of the soybean is an effective gene capable of regulating and controlling the nodulation quantity of specific rhizobium on the soybean, and can regulate and control the nodulation quantity by directly identifying the haplotype of slow-growing rhizobium specific effect protein NopP to limit the symbiotic nodulation of indigenous rhizobium, so that the soybean preferentially nodulates with artificially applied efficient rhizobium inoculant; and the symbiotic nitrogen fixation capability can be improved.

The invention discloses an estrogen receptor promoting gene FERR and an estrogen receptor restraining gene FERR-R of populus deltoides and an application of the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of the populus deltoides, and belongs to the technical field of plant genetic engineering. According to the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of populus deltoides and an application of the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of the populus deltoides disclosed by the invention, through genealogy linkage analysis, sex determination gene sites of the populus deltoides are subjected to fine positioning, then through natural population materials,complete genome correlation analysis is developed, methylation sequencing of transcriptomes and genomes is combined, the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of poplars are disclosed for the first time, the FERR-R gene does not code protein, but through producing siRNAs, a starting sequence of the estrogen receptor promoting gene FERR is subjected to methylation, and transcripts are sheared, so that the expression of the estrogen receptor promoting gene FERR in poplar male plants is restrained. But poplar female plants do not contain the FERR-R gene, and the expression of the FERR is not restrained by the FERR-R, so that the development of female flowers of the female plants is promoted. The estrogen receptor promoting gene and the estrogen receptor restraining gene of the poplars provided by the invention can be applied to early identification of the sex of the poplar and molecular breeding.

The invention provides a simple and efficient potato multi-R gene polymerization assisted breeding method. The method specifically comprises the following steps of obtaining late blight resistance screening primer sequence groups RB, R8, Rx1, Ryadg and GBSS, constructing a multiple PCR reaction system by using the primer group, and carrying out pyramiding breeding selection on late blight lastingresistance and PVX and PVY virus disease resistance genes. The method is high in detection specificity, has good tolerance to the quality and concentration of a DNA template, and is convenient in adaptation to screening and identification of molecular markers of large-scale breeding intermediate materials. The method provides a simple and efficient technology for late blight and virus disease polyclonal antibody gene pyramiding breeding and provides a technical scheme for breeding of new polyclonal antibody varieties.

The invention relates to a transcription factor for enhancing broad-spectrum disease resistance of plants and an application. The invention discloses that PIBP1 directly interacts with PigmR and otherbroad-spectrum anti-disease R genes to promote accumulation of own cell nuclear proteins and positively regulate and control disease resistance of plants. The PIBP1 and the homologous gene are combined as a kind of non-classical transcription factors and activate expression of defensive genes such as OsWAK14 and OsPAL1 at the downstream of the non-classical transcription factors. Therefore, the invention provides a new transcription factor which activates a downstream disease-resistant gene through direct interaction with the R gene and endows plants with a new mechanism of disease resistance. The invention not only provides a practical and effective plant improvement method, but also provides a new way for broad-spectrum immune mechanism research of plants and disease resistance breedingof plants.

The invention discloses a monorhamnolipid production strain and an application thereof, and belongs to the technical field of biology. The pseudomonas aeruginosa PAO1 strain is used as an original strain, the rhlC gene of the pseudomonas aeruginosa PAO1 strain is replaced with an rhlAB-R gene cluster through a homologous recombination method, and the pseudomonas aeruginosa strain capable of specifically producing monorhamnolipid is obtained. The random mutation is further combined, and a mutant strain with the single rhamnolipid production level remarkably improved is screened out. According to the mutant strain, commercially available goldfish sunflower seed oil is used as a substrate, fermentation is carried out in a 5L bioreactor at 37 DEG C for 90 hours, the concentration of monorhamnolipid reaches 62.7 g/L, the purity reaches 95.16%, and the ratio of Rha-C10-C10 is the maximum and accounts for 68.59%.

Compositions and methods and for enhancing the resistance of plants to plant diseases caused by potyviruses are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of a plant to plant disease caused by a potyvirus comprise introducing a nucleic acid molecule encoding an R gene product into a plant cell. Additionally provided are methods for using the plants in agriculture to limit plant disease.

Compositions and methods and for enhancing the resistance of wheat plants to wheat stem rust caused by Puccinia graminis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of a wheat plant to wheat stem mst comprise introducing a nucleic acid molecule encoding an R gene product into a wheat plant cell. Additionally provided are methods for using the wheat plants in agriculture to limit wheat stem rust.

The invention relates to the field of plant transformation, in particular plant transformation of a Solanaceae, preferably of potato. The present invention provides an alternative selection method inplant transformation processes. The invention further provides a plant that has been provided with additional nucleic acid sequences but which genetically modified plant essentially consists of cis plant sequences, for example a genetically modified potato plant that has been provided with additional (essentially) potato plant sequences. Such a transgenic plant is free from non-Solanum T-DNA border sequences. A preferred embodiment is a potato plant that carries a functional R-gene, providing resistance against an oomycete pathogen, preferably Phytophthora infestans, wherein said R-gene can beused as selectable marker.