Estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides and application of estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides

A technology of gene and black poplar, applied in the field of plant genetic engineering, can solve the problem that the sex-determining gene has not been cloned and determined

Active Publication Date: 2020-05-05
NANJING FORESTRY UNIV
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Problems solved by technology

But the sex-determining gene of poplar has not been cloned and determined

Method used

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  • Estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides and application of estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides
  • Estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides and application of estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides
  • Estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides and application of estrogen receptor promoting gene FERR and estrogen receptor restraining gene FERR-R of populus deltoides

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Embodiment 1

[0042] (1) Populus nigra full-sib population construction and preliminary sex-determining gene mapping

[0043] In 2012, we constructed a full-sib population of Populus americana consisting of 1077 offspring. The population was planted in Sihong Forest Farm, Jiangsu Province with a row spacing of 6×6 meters, and the population had reached sexual maturity. A genetic map covering the whole genome was constructed for this population, some individuals were randomly selected from the mapping population, and a genome-wide scan of the sex-determining genes of poplar was performed. Sex-determining genes are located at the end of chromosome 19, between the telomere and the N110 marker ( figure 1 ). Sex linkage analysis also found that the sex-determining genes of poplar were heterozygous for males, which indicated that the sex determination system of Populus nigra was XY.

[0044] (2) Fine mapping of sex-determining genes and construction of chromosomal haplotypes in sex-determining ...

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Abstract

The invention discloses an estrogen receptor promoting gene FERR and an estrogen receptor restraining gene FERR-R of populus deltoides and an application of the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of the populus deltoides, and belongs to the technical field of plant genetic engineering. According to the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of populus deltoides and an application of the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of the populus deltoides disclosed by the invention, through genealogy linkage analysis, sex determination gene sites of the populus deltoides are subjected to fine positioning, then through natural population materials,complete genome correlation analysis is developed, methylation sequencing of transcriptomes and genomes is combined, the estrogen receptor promoting gene FERR and the estrogen receptor restraining gene FERR-R of poplars are disclosed for the first time, the FERR-R gene does not code protein, but through producing siRNAs, a starting sequence of the estrogen receptor promoting gene FERR is subjected to methylation, and transcripts are sheared, so that the expression of the estrogen receptor promoting gene FERR in poplar male plants is restrained. But poplar female plants do not contain the FERR-R gene, and the expression of the FERR is not restrained by the FERR-R, so that the development of female flowers of the female plants is promoted. The estrogen receptor promoting gene and the estrogen receptor restraining gene of the poplars provided by the invention can be applied to early identification of the sex of the poplar and molecular breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and more specifically relates to an estrogen-promoting gene FERR and an estrogen-repressing gene FERR-R of American black poplar (Poplusdeltoides) and applications thereof. Background technique [0002] The hypothesis of the origin of plant sex chromosomes holds that sex chromosomes originate from a pair of autosomes. The evolution of the sex chromosomes begins with a male or female sterile mutation on one chromosome of the pair of autosomes. The hypothesis of the origin of plant sex chromosomes holds that sex chromosomes originate from a pair of autosomes. The evolution of sex chromosomes begins with a male or female sterile mutation on one chromosome of the pair of autosomes. Due to some unknown reason, the recombination of the mutation site is inhibited, and the non-recombination region gradually expands to form a heterozygous sex chromosome. Dioecious plants are evolutiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/02C12Q1/6895
CPCC07K14/415C12N15/827C12Q1/6895
Inventor 尹佟明薛良交吴怀通陈赢男陆静李小平侯静戴晓港
Owner NANJING FORESTRY UNIV
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