23 results about "Gene mapping" patented technology

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a KASP molecular marker-assisted breeding method for a wilt-resistant bottle gourd variety. The invention firstly provides a pair of molecular markers closely linked with the wilt resistance gene of the bottle gourd, the molecular markers are KASP markers, and the molecular markers comprise rs51160 and rs51106; the SNP site of the rs51160 is located at the 12273198 bp position of the chromosome 8 of the bottle gourd genome, the polymorphism is C / T, the SNP site of the rs51106 is located at the 12007808 bp position of the chromosome 8 of the bottle gourd genome, and the polymorphism is A / G. The method comprises the following steps: firstly, obtaining a KASP marker closely linked with a wilt-resistant gene by utilizing a gene positioning method, further amplifying a material needing to be identified by utilizing the marker, and judging whether single plants carry a wilt-resistant target gene or not according to the genotype of the material, so that the disease-resistant breeding process of offspring groups is accelerated.

The present invention provides an interpretable breeding recommendation method based on crop trait and gene mapping analysis, which belongs to the field of breeding recommendation technology based on computer data processing. First, multi-angle images of crops are collected, and their corresponding SNP genomic markers are collected at the same time; secondly, an entity-level crop trait feature extraction model is designed to realize the identification of key traits and spatial position output of each crop. Subsequently, models are constructed for continuous traits and discrete traits respectively, and a contribution score table of SNP sites to each trait is obtained, and the trait target evaluation baseline data is generated by combining the reference genotype and the unit contribution change value. Finally, combined with the target trait configuration input by the user, a multi-objective loss function is constructed and optimized to generate the optimal SNP gene combination scheme. An intelligent breeding method with the ability of trait identification, gene contribution quantification and interpretable gene combination optimization is provided, which meets the comprehensive technical needs of modern breeding engineering.

The present invention discloses SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs, their primers and applications, belonging to the technical field of molecular genetics. The SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs disclosed by the present invention have amplification primer sequences as shown in SEQ ID NO.1 and 2. The SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs and their amplification primers can be applied to the population structure analysis of Corydalis bulbocapniodes, the analysis of markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs, the selection of excellent germplasms of Corydalis bulbocapniodes and the preparation of a kit for screening excellent germplasms of Corydalis bulbocapniodes with high sub-bulb weight, and can be directly used for screening excellent germplasms, gene mapping and cloning, and molecular marker-assisted breeding. Moreover, they have chromosome mapping information, which is beneficial to further fine mapping and cloning of genes controlling the fresh weight trait of sub-bulbs, and is of great significance in the breeding of high-yield Corydalis bulbocapniodes.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

This invention discloses a combination of SNP molecular markers for sweet potato, an SNP chip, and their applications, relating to the fields of plant biotechnology and plant molecular breeding. The chip contains 16,730 SNP loci located on chromosome 15 of the sweet potato reference genome "Y22". This sweet potato 16K liquid-phase SNP chip, SweetpotatoGBTS16K, is applied to genotyping, variety identification, gene mapping, and genome-wide association analysis of sweet potato varieties. The SNP loci on the liquid-phase chip of this invention were screened from large-scale sweet potato genome resequencing data, totaling 16,730 SNP loci, encompassing associated loci for major agronomic traits such as yield, quality, and resistance in sweet potato, and has broad application prospects in multiple fields of sweet potato breeding.

The present invention discloses a 60K SNP liquid breeding chip for broiler chickens, which relates to the field of biological detection technology. The 60K SNP liquid breeding chip for broiler chickens includes a probe combination, which is used to identify the genotypes of 61,219 SNP loci. The functional loci and gene region loci of the 60K SNP liquid breeding chip for broiler chickens provided by the present invention are rich, the chip loci have high polymorphism and are evenly distributed on chromosomes, and it can be widely used in different application scenarios such as the construction of genetic maps of broiler chickens, gene mapping, genome-wide association analysis, molecular marker-assisted selection, directional improvement, mining and identification of genes for important traits and functional analysis, and genome-wide selection breeding for different broiler chicken samples, which is of great significance for improving the breeding efficiency of broiler chickens and promoting the high-quality development of the broiler chicken industry in China.

The present invention relates to the technical field of molecular detection. Disclosed are a Camellia sinensis liquid-phase chip and the use thereof. Disclosed in the present invention are a Camellia sinensis liquid-phase chip and the use thereof. According to locus screening requirements and probe design principles, the chip comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip of the present invention can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip of the present invention can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

The present invention discloses a Chinese pumpkin SNP molecular marker combination, a SNP chip, and their applications, relating to the field of biotechnology. The pumpkin SNP molecular marker combination includes 20,213 SNP molecular markers, whose loci are determined by sequence alignment of the pumpkin reference genome "Cmoschata_genome_v1.fa.gz," as shown in Table 1 of the specification. The combination can be used for molecular marker fingerprint analysis of pumpkin cultivar resources, genotype identification of hybrid population progeny, authenticity verification of varieties, genetic background analysis and screening of breeding materials, genome-wide association analysis, genetic diversity analysis of germplasm resources, and kinship identification, providing an indispensable tool for high-throughput pumpkin genotyping, gene mapping, fingerprinting, genome-wide selection, and other molecular breeding research.

The present invention provides high-content and high-resolution in vivo screens for mapping genes that are linked to changes in cytoarchitecture or spatial transcriptomics of brain cells (e.g., neurons). The multiplex gene mapping platform utilizes genetic perturbations that are delivered to a CRISPR-expressing transgenic system via viral vectors (e.g., lentiviral vectors or AAV vectors). Phenotypic changes in the cytoarchitecture or spatial transcriptomics resulting from genetic perturbations are examined in individual brain cells, which can then be correlated with specific target genes that are modulated by the genetic perturbations.

The present invention discloses SSR molecular marker primers associated with the number trait of Corydalis yanhusuo tubers and their applications, belonging to the technical field of molecular genetics. The SSR molecular marker primers associated with the number trait of Corydalis yanhusuo tubers disclosed by the present invention have nucleotide sequences as shown in SEQ ID NO.1 and 2. The SSR molecular markers associated with the number trait of Corydalis yanhusuo tubers and their amplification primers can be applied to the population structure analysis of Corydalis yanhusuo, the analysis of markers associated with the number trait of Corydalis yanhusuo tubers, the selection of excellent germplasms of Corydalis yanhusuo, and the preparation of kits for identifying the number of Corydalis yanhusuo tubers, and can be directly used for screening excellent germplasms, gene mapping and cloning, and molecular marker-assisted breeding. Moreover, they have chromosome mapping information, which is beneficial to further fine mapping and cloning of genes controlling the number trait of tubers, and is of great significance in the breeding of high-yield Corydalis yanhusuo.

The invention relates to a new strain JSJ-J2HD2-HD3 of Naematia auramtiala, which is bred by a space breeding technology, and an application of the new strain JSJ-J2HD2-HD3, and belongs to the technical field of edible mushroom breeding. The new strain of the tremella aurantialba is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.41788. The new strain of the tremella aurantialba and a stereum hirsutum strain JSJ-G2YX001C which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.41790 are cultured and bred under a dark culture condition to obtain a yellow tremella aurantialba sporocarp strain. The novel tremella aurantialba strain can omit color changing conditions, save production cost and improve production benefits, can be used as an excellent strain resource for genetic breeding of tremella aurantialba species, researches color-related gene information, and provides an important theoretical basis for gene positioning breeding.

The invention provides an interpretable breeding recommendation method based on crop traits and gene mapping analysis, and belongs to the technical field of breeding recommendation based on computer data processing. The method comprises the following steps: firstly, collecting multi-angle images of crops, and meanwhile, collecting corresponding SNP genome markers; secondly, designing an entity-level crop character feature extraction model, and realizing key character recognition and spatial position output of each crop; and then, respectively constructing models for continuous characters and discrete characters, obtaining a contribution degree score table of the SNP sites to each character, and generating character target evaluation baseline data in combination with a reference genotype and a unit contribution degree change value. And finally, in combination with target character configuration input by a user, constructing a multi-target loss function and performing optimization solution to generate an optimal SNP gene combination scheme. The invention provides an intelligent breeding method with the capabilities of character recognition, gene contribution degree quantification and interpretable gene combination optimization, and meets the comprehensive technical requirements of modern breeding engineering.

The invention relates to a new strain JSJ-J2HD40-HD13 of Naematia auramtiala, which is bred by a space breeding technology, and an application of the new strain JSJ-J2HD40-HD13, and belongs to the technical field of edible mushroom breeding. The new strain of the tremella aurantialba is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.41789, and the tremella aurantialba and a stereum hirsutum strain JSJ-G2YX001C which is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.41790 are cultured and bred under a dark culture condition to obtain a full-yellow tremella aurantialba sporocarp strain. The novel tremella aurantialba strain can omit color changing conditions, save production cost and improve production benefits, can be used as an excellent strain resource for genetic breeding of tremella aurantialba species, researches color-related gene information, and provides an important theoretical basis for gene positioning breeding.

The invention provides a dynamic weight and cross-species data time sequence modeling and scoring method and a gene combination, and belongs to the technical field of biological information processing and data modeling. According to the technical scheme, on the basis of multi-source group data, through causal network construction, time sequence interpolation and multi-stage model training, feature genes closely related to the stroke process are screened out, the dynamic weight function is constructed, and a scoring system used for staging analysis is formed; according to the method, cross-species gene mapping and dynamic data fusion can be carried out in combination with mouse models and human data, scoring results can correspond to multiple key stages of stroke, and the method has the advantages of being high in expression stability, clear in dynamic trend, good in model interpretation and the like, meanwhile has good universality and is suitable for popularization and application. The method can be expanded and applied to other complex immune process modeling tasks, and has high adaptability, interpretability and popularization potential.

This invention discloses a cowpea SNP molecular marker combination, belonging to the field of molecular breeding technology. The cowpea SNP molecular marker combination consists of 53,131 SNP molecular markers. The physical locations of these 53,131 SNP molecular markers are determined based on alignment with the cowpea reference genome IT97K-499-35, as shown in Table 1 of the specification. Compared with existing technologies, this SNP molecular marker combination is evenly distributed across the 11 chromosomes of cowpea, exhibiting high polymorphism and detection rate. This invention also provides an SNP liquid phase chip for detecting the cowpea SNP molecular marker combination, providing theoretical and data support for cowpea new variety identification, gene mapping, molecular marker-assisted selection breeding, genome-wide selection, and population genetic diversity analysis, possessing significant theoretical value and application significance.

Provided are a Camellia sinensis liquid-phase chip and the use thereof, relating to the technical field of molecular detection. The Camellia sinensis liquid-phase chip is designed according to locus screening requirements and probe design principles, comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

A sesame shattering resistance trait regulation gene Sihec3 and its application are provided. This gene is located on the third chromosome of sesame, is a recessive control gene, and fully explains the phenotypic variation of the shattering resistance trait. Genetic analysis of the shattering trait was conducted with F2 and F2:3 populations derived from a hybrid combination between a shattering resistance mutant M7 and normal shattering material. The research indicates that the seed shattering resistance trait in the tested population is a recessive trait controlled by a single gene. Based on the self-constructed sesame genome fine map and efficient gene mapping technology, the key gene Sihec3 and its allele SiHEC3 regulating the sesame shattering trait were cloned and compared. A corresponding gene marker named SSR1 for the sesame shattering resistance trait was dig. This invention provides a technical foundation for breeding new sesame varieties with shattering resistance trait.

The invention discloses a plant genetic transformation method based on a haploid induction line. According to the invention, a haploid immature embryo obtained by utilizing a haploid induction line containing a visual marker is transformed, so that a rapid homozygosis method of transgenic and gene editing plants is realized; comprising the following steps: (1) hybridizing a homozygous haploid induction line with an embryo part color mark as a male parent with other varieties of plants to obtain hybrid F1-generation seeds, and identifying haploid and diploid immature embryos according to the embryo part color of the F1-generation seeds; and 2) carrying out genetic transformation on the haploid immature embryo to obtain a T0-generation haploid transgenic plant, and doubling the obtained T0-generation haploid transgenic plant by using a doubling reagent so as to rapidly obtain a homozygous transgenic or gene editing plant. The method disclosed by the invention has important significance on wheat haploid breeding, DH population construction, gene positioning, target gene function research and the like.

This application discloses the application of KASP molecular markers in identifying the red / yellow skin trait in melons, belonging to the field of molecular marker technology. It aims to utilize molecular marker-assisted selection breeding for the red / yellow skin trait in melons to promote gene mapping and molecular-assisted breeding processes. A method for identifying the red / yellow skin trait in melons includes the following steps: extracting genomic DNA from the target melon; performing PCR amplification using the genomic DNA as a template; and performing fluorescence detection based on primer pairs of KASP molecular markers RY-1 or RY-2 to obtain the gene type of the target melon; and determining the trait of the target melon based on the detected gene type; wherein, KASP molecular markers RY-1 and RY-2 are designed based on SNP sites at Chr04:469552 and Chr04:760234, respectively. The identification method in this application can be more efficiently applied to fields such as molecular marker-assisted breeding of melons, variety purity detection, and gene editing target screening.

The embodiments of the present application disclose KASP molecular markers, primer pairs, kits and applications thereof for identifying dwarf traits of melons, which relate to the field of molecular marker technology and aim to utilize molecular marker-assisted selection breeding in melon dwarf traits to promote gene localization and molecular-assisted breeding processes. The method for identifying dwarf traits of melons comprises the following steps: extracting genomic DNA of a target melon, using the genomic DNA of the target melon as a template for PCR amplification, and performing fluorescence detection based on primers of KASP marker K1069930 to obtain the gene type of the target melon; based on the detected gene type, determining the trait of the target melon; the KASP marker K1069930 is designed based on the SNP site at Chr07:1069930, and the nucleotide sequence of the SNP site is shown in SEQ ID NO.1. The identification method in the present application can be more efficiently applied to the breeding of dwarf germplasm of melons.