6 results about "Gene mapping" patented technology

This invention discloses a combination of SNP molecular markers for sweet potato, an SNP chip, and their applications, relating to the fields of plant biotechnology and plant molecular breeding. The chip contains 16,730 SNP loci located on chromosome 15 of the sweet potato reference genome "Y22". This sweet potato 16K liquid-phase SNP chip, SweetpotatoGBTS16K, is applied to genotyping, variety identification, gene mapping, and genome-wide association analysis of sweet potato varieties. The SNP loci on the liquid-phase chip of this invention were screened from large-scale sweet potato genome resequencing data, totaling 16,730 SNP loci, encompassing associated loci for major agronomic traits such as yield, quality, and resistance in sweet potato, and has broad application prospects in multiple fields of sweet potato breeding.

The present invention relates to the technical field of molecular detection. Disclosed are a Camellia sinensis liquid-phase chip and the use thereof. Disclosed in the present invention are a Camellia sinensis liquid-phase chip and the use thereof. According to locus screening requirements and probe design principles, the chip comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip of the present invention can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip of the present invention can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

The present invention provides high-content and high-resolution in vivo screens for mapping genes that are linked to changes in cytoarchitecture or spatial transcriptomics of brain cells (e.g., neurons). The multiplex gene mapping platform utilizes genetic perturbations that are delivered to a CRISPR-expressing transgenic system via viral vectors (e.g., lentiviral vectors or AAV vectors). Phenotypic changes in the cytoarchitecture or spatial transcriptomics resulting from genetic perturbations are examined in individual brain cells, which can then be correlated with specific target genes that are modulated by the genetic perturbations.

Provided are a Camellia sinensis liquid-phase chip and the use thereof, relating to the technical field of molecular detection. The Camellia sinensis liquid-phase chip is designed according to locus screening requirements and probe design principles, comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

A sesame shattering resistance trait regulation gene Sihec3 and its application are provided. This gene is located on the third chromosome of sesame, is a recessive control gene, and fully explains the phenotypic variation of the shattering resistance trait. Genetic analysis of the shattering trait was conducted with F2 and F2:3 populations derived from a hybrid combination between a shattering resistance mutant M7 and normal shattering material. The research indicates that the seed shattering resistance trait in the tested population is a recessive trait controlled by a single gene. Based on the self-constructed sesame genome fine map and efficient gene mapping technology, the key gene Sihec3 and its allele SiHEC3 regulating the sesame shattering trait were cloned and compared. A corresponding gene marker named SSR1 for the sesame shattering resistance trait was dig. This invention provides a technical foundation for breeding new sesame varieties with shattering resistance trait.