14 results about "Gene mapping" patented technology

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a KASP molecular marker-assisted breeding method for a wilt-resistant bottle gourd variety. The invention firstly provides a pair of molecular markers closely linked with the wilt resistance gene of the bottle gourd, the molecular markers are KASP markers, and the molecular markers comprise rs51160 and rs51106; the SNP site of the rs51160 is located at the 12273198 bp position of the chromosome 8 of the bottle gourd genome, the polymorphism is C / T, the SNP site of the rs51106 is located at the 12007808 bp position of the chromosome 8 of the bottle gourd genome, and the polymorphism is A / G. The method comprises the following steps: firstly, obtaining a KASP marker closely linked with a wilt-resistant gene by utilizing a gene positioning method, further amplifying a material needing to be identified by utilizing the marker, and judging whether single plants carry a wilt-resistant target gene or not according to the genotype of the material, so that the disease-resistant breeding process of offspring groups is accelerated.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

This invention discloses a combination of SNP molecular markers for sweet potato, an SNP chip, and their applications, relating to the fields of plant biotechnology and plant molecular breeding. The chip contains 16,730 SNP loci located on chromosome 15 of the sweet potato reference genome "Y22". This sweet potato 16K liquid-phase SNP chip, SweetpotatoGBTS16K, is applied to genotyping, variety identification, gene mapping, and genome-wide association analysis of sweet potato varieties. The SNP loci on the liquid-phase chip of this invention were screened from large-scale sweet potato genome resequencing data, totaling 16,730 SNP loci, encompassing associated loci for major agronomic traits such as yield, quality, and resistance in sweet potato, and has broad application prospects in multiple fields of sweet potato breeding.

The present invention relates to the technical field of molecular detection. Disclosed are a Camellia sinensis liquid-phase chip and the use thereof. Disclosed in the present invention are a Camellia sinensis liquid-phase chip and the use thereof. According to locus screening requirements and probe design principles, the chip comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip of the present invention can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip of the present invention can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

The present invention discloses a Chinese pumpkin SNP molecular marker combination, a SNP chip, and their applications, relating to the field of biotechnology. The pumpkin SNP molecular marker combination includes 20,213 SNP molecular markers, whose loci are determined by sequence alignment of the pumpkin reference genome "Cmoschata_genome_v1.fa.gz," as shown in Table 1 of the specification. The combination can be used for molecular marker fingerprint analysis of pumpkin cultivar resources, genotype identification of hybrid population progeny, authenticity verification of varieties, genetic background analysis and screening of breeding materials, genome-wide association analysis, genetic diversity analysis of germplasm resources, and kinship identification, providing an indispensable tool for high-throughput pumpkin genotyping, gene mapping, fingerprinting, genome-wide selection, and other molecular breeding research.

The present invention provides high-content and high-resolution in vivo screens for mapping genes that are linked to changes in cytoarchitecture or spatial transcriptomics of brain cells (e.g., neurons). The multiplex gene mapping platform utilizes genetic perturbations that are delivered to a CRISPR-expressing transgenic system via viral vectors (e.g., lentiviral vectors or AAV vectors). Phenotypic changes in the cytoarchitecture or spatial transcriptomics resulting from genetic perturbations are examined in individual brain cells, which can then be correlated with specific target genes that are modulated by the genetic perturbations.

The invention provides a dynamic weight and cross-species data time sequence modeling and scoring method and a gene combination, and belongs to the technical field of biological information processing and data modeling. According to the technical scheme, on the basis of multi-source group data, through causal network construction, time sequence interpolation and multi-stage model training, feature genes closely related to the stroke process are screened out, the dynamic weight function is constructed, and a scoring system used for staging analysis is formed; according to the method, cross-species gene mapping and dynamic data fusion can be carried out in combination with mouse models and human data, scoring results can correspond to multiple key stages of stroke, and the method has the advantages of being high in expression stability, clear in dynamic trend, good in model interpretation and the like, meanwhile has good universality and is suitable for popularization and application. The method can be expanded and applied to other complex immune process modeling tasks, and has high adaptability, interpretability and popularization potential.

This invention discloses a cowpea SNP molecular marker combination, belonging to the field of molecular breeding technology. The cowpea SNP molecular marker combination consists of 53,131 SNP molecular markers. The physical locations of these 53,131 SNP molecular markers are determined based on alignment with the cowpea reference genome IT97K-499-35, as shown in Table 1 of the specification. Compared with existing technologies, this SNP molecular marker combination is evenly distributed across the 11 chromosomes of cowpea, exhibiting high polymorphism and detection rate. This invention also provides an SNP liquid phase chip for detecting the cowpea SNP molecular marker combination, providing theoretical and data support for cowpea new variety identification, gene mapping, molecular marker-assisted selection breeding, genome-wide selection, and population genetic diversity analysis, possessing significant theoretical value and application significance.

Provided are a Camellia sinensis liquid-phase chip and the use thereof, relating to the technical field of molecular detection. The Camellia sinensis liquid-phase chip is designed according to locus screening requirements and probe design principles, comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

A sesame shattering resistance trait regulation gene Sihec3 and its application are provided. This gene is located on the third chromosome of sesame, is a recessive control gene, and fully explains the phenotypic variation of the shattering resistance trait. Genetic analysis of the shattering trait was conducted with F2 and F2:3 populations derived from a hybrid combination between a shattering resistance mutant M7 and normal shattering material. The research indicates that the seed shattering resistance trait in the tested population is a recessive trait controlled by a single gene. Based on the self-constructed sesame genome fine map and efficient gene mapping technology, the key gene Sihec3 and its allele SiHEC3 regulating the sesame shattering trait were cloned and compared. A corresponding gene marker named SSR1 for the sesame shattering resistance trait was dig. This invention provides a technical foundation for breeding new sesame varieties with shattering resistance trait.

The invention discloses a plant genetic transformation method based on a haploid induction line. According to the invention, a haploid immature embryo obtained by utilizing a haploid induction line containing a visual marker is transformed, so that a rapid homozygosis method of transgenic and gene editing plants is realized; comprising the following steps: (1) hybridizing a homozygous haploid induction line with an embryo part color mark as a male parent with other varieties of plants to obtain hybrid F1-generation seeds, and identifying haploid and diploid immature embryos according to the embryo part color of the F1-generation seeds; and 2) carrying out genetic transformation on the haploid immature embryo to obtain a T0-generation haploid transgenic plant, and doubling the obtained T0-generation haploid transgenic plant by using a doubling reagent so as to rapidly obtain a homozygous transgenic or gene editing plant. The method disclosed by the invention has important significance on wheat haploid breeding, DH population construction, gene positioning, target gene function research and the like.

This application discloses the application of KASP molecular markers in identifying the red / yellow skin trait in melons, belonging to the field of molecular marker technology. It aims to utilize molecular marker-assisted selection breeding for the red / yellow skin trait in melons to promote gene mapping and molecular-assisted breeding processes. A method for identifying the red / yellow skin trait in melons includes the following steps: extracting genomic DNA from the target melon; performing PCR amplification using the genomic DNA as a template; and performing fluorescence detection based on primer pairs of KASP molecular markers RY-1 or RY-2 to obtain the gene type of the target melon; and determining the trait of the target melon based on the detected gene type; wherein, KASP molecular markers RY-1 and RY-2 are designed based on SNP sites at Chr04:469552 and Chr04:760234, respectively. The identification method in this application can be more efficiently applied to fields such as molecular marker-assisted breeding of melons, variety purity detection, and gene editing target screening.