112 results about "Transformation cell" patented technology

The invention is based on the discovery of methods for purification of an acid active hyaluronidase found in human plasma (hpHAse), including both biochemical and immunoaffinity purification methods. The method of immunoaffinity purification of the invention is based on the discovery of a method for identifying antibodies that specifically bind native hpHAse (anti-native hpHAse antibodies), and anti-native hpHAse antibodies identified by this screening method. The invention also features an assay for sensitive detection of HAse activity using biotinylated hyaluronic acid (bHA). Purification and characterization of hpHAse lead to the inventors' additional discovery that hpHAse is encoded by the LuCa-1 gene, which gene is present in the human chromosome at 3p21.3, a region associated with tumor suppression. The invention additionally features methods of treating tumor-bearing patients by administration of hpHAse and / or transformation of cells with hpHAse-encoding DNA.

A co-culture apparatus and method for, among other things, converting a progenitor cell line into a target cell line. The apparatus comprises an exterior receptacle, a removable cartridge that fits into the exterior receptacle, and a closure member to seal the apparatus. The invention is also directed to a method for transforming a progenitor cell line into a target cell line. In one embodiment of the invention, the method comprises the steps of: establishing a progenitor cell line; establishing a support cell line; co-culturing said progenitor and support cell lines; establishing a transformation cell line; and co-culturing said progenitor and transformation cell lines, wherein the transformation cell line supports the transformation of the progenitor cell line into a target cell line.

The present invention provides efficient transfer of genes into host cells or embryos to transform the cells or embryos by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may also be developed into transgenic organisms.

This invention describes a new O-Conotoxin (O-CTX), its encoding polynucleotide, the construction units and transformation cells having this encoding polynucleotide, and the reconstruction process for producing the said O-Conotoxin. This invention also describes the artificial synthesis and applications of the said O-Conotoxin.

The present application discloses a method for discovering a methylation marker gene for the conversion of a cell comprising: (i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell; (ii) treating a converted cell with a demethylating agent and comparing its gene expression content with gene expression content of an untreated converted cell to identify a gene that is present in greater abundance in the cell treated with the demethylating agent; and (iii) identifying a gene that is common to the identified genes in steps (i) and (ii), wherein the common identified gene is the methylation marker gene.

An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.

The invention is generally related to methods of generating plants transformed with novel autonomous mini-chromosomes. Mini-chromosomes with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the mini-chromosome into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the mini-chromosome. Plants generated in the present invention contain novel genes introduced into their genome by integration into existing chromosomes.

The present invention provides methods of introducing a polynucleotide into a target cell, wherein the method employs a light generating protein coding sequence acting as a reporter. An important advantage of the methods described herein is that drug resistant target cells or target cells having no useful auxotrophic markers can be effectively transformed. The present invention also includes transformed cells produced by the methods described herein. Also described are light generating protein coding sequence modifications, a variety of vectors, and methods of using the transformed cells of the present invention.

This invention describes a new T-CTX, its encoding polynucleotide, the construction units and transformation cells having this encoding polynucleotide, and the reconstruction process for producing the said T-CTX. This invention also describes the artificial synthesis and applications of the said T-CTX.

The invention is generally related to methods of generating plants transformed with novel autonomous mini-chromosomes. Mini-chromosomes with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the mini-chromosome into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the mini-chromosome. Plants generated in the present invention contain novel genes introduced into their genome by integration into existing chromosomes.

The invention discloses a soybean gene GmCIB1 and a gene GmCRY2 and application thereof for regulating and controlling flowering and aging, and particularly relates to a soybean gene GmCIB1 and a gene GmCRY2 with sequences shown in SEQ ID NO: 1 or 3, a DNA (deoxyribonucleic acid) molecule containing the genes, a carrier, protein encoded by the gene and related transformation cells and transgenic plants. The invention also relates to application of the genes in regulating and controlling plant leaf aging and flowering, and a method for culturing related transgenic plants.

The invention provides a three-dimensional storage device array structure and a manufacturing method thereof. The three-dimensional storage array is formed by the derivation of grid control PNPN vertical selection tubes connected in series mutually and a storage cell of a resistant transformation or phase transformation cell in a three-dimensional space. The cross interference and leakage of electricity problems between adjacent units of the storage array in operation are improved effectively through the storage cell with high switching current ratio, also the structure of the storage cell issimplified, the multi-layer stacking is realized, and the storage density is improved.

The present invention provides one method of using methanol utilizing yeast, especially transferred recombinant saccharomycete with ectogenic S-adenosyl-menthionine (SAM) synthetase expression vector, to produce SAM and the method of constituting the expression vector and transferred cell. The recombinant saccharomycete is used in fermentation of culture medium with methanol, proper amount of methionine and certain nitrogen source, high yield of SAM may be obtain and SAM content in cell may reach 10-20 % of dry cell weight. The present invention also provides one methanol utilizing Pachia pastoris sacchromycete strain with preserving number of CGMCC No.0648.

The present invention relates to a spider silk crystal protein gene synthesized by plant preferance codon, its expression carrier, the plant cells transformed from it, and the transgenic plant and its descendant. Said gene can be effectively expressed in fibrous plant and the obtained protein moleculae can be combined with the cellulose of plant, so improving the strength of plant fibres.

Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is / are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.

A variety of genetic constructs are disclosed that will find both in vitro and in vivo use in the area of tumor biology and cancer therapy. In particular, expression constructs are provided that contain a p16 encoding region and other regulatory elements necessary for the expression of a p16 transcript. One version of the expression construct is a replication-deficient adenoviral vector. Also provided are methods for the transformation of cell lines and the inhibition of cancer cell proliferation.

It is an object of the present invention to provide an inexpensive and efficient industrial method for obtaining (S)-2-pentanol, (S)-2-hexanol, 1-methylalkyl malonic acid and 3-methyl carboxylic acid at a high optical purity. The present invention provides a method of producing (S)-2-pentanol or (S)-2-hexanol which comprises allowing certain types of microorganisms or transformed cells, a product obtained by treating said microorganisms or cells, a culture solution of said microorganisms or cells, and / or a crude purified product or purified product of a carbonyl reductase fraction obtained from said microorganisms or cells, to act on 2-pentanone or 2-hexanone.

This invention relates to a biological agent testing blue tongue of animals and its preparation method. The agent includes blue tongue virus VP7 gene recombination expression plasmid vectors and a blue tongue virus VPT recombination antigen got from its expression. The preparation method includes: 1, cloning BTV coding group specific antigen VP7 gene fragment to pMD18-T plasmid vector to make up of VP7 gene clone recombination plasmid, 2, sub-cloning plug pBAD / Thio TOPO expression vector, 3, converting TOP10cells, 4, screening the positive clones obtaining BTV VP7 gene segment forward plug with correct read code frame to set up BTV group specific antigen VP7 recombination expression vector 5, cultivating the vector with LB culture media containing 100mug / ml Amp.

The present invention relates to methods and compositions comprising small interfering RNAs (siRNAs) to identify nucleotides encoding proteins involved in the co-transport of inorganic phosphate in vascular tissues. Specifically, it relates to the identification of Pit-1 and Pit-2 as key proteins involved in vascular calcification, the deposition of calcium phosphate, in blood vessels. The nucleic acids encoding specific siRNAs and vectors encoding these siRNAs are useful as tools to specifically inhibit the expression of Pit-1, Pit-2, or related proteins in various primary and stably-transformed cell lines, and as tools to explore the roles and functional relationships of these proteins involved in vascular calcification. In addition, they may be useful directly as therapeutic agents in humans delivered to sites of vascular calcification.

The invention discloses a high-efficiency digital optical fiber CDMA (Code Division Multiple Access) repeater and a realizing method. The system consists of a near terminal device and a remote terminal device, and the near terminal device and the remote terminal device communicate with each other through transmitting digital base band signals in optical fibers. The repeater comprises a radio frequency small signal cell, a digital signal process cell, a highly efficient power amplification cell, a duplexer cell, a voltage transformation cell, a monitor cell and the like, wherein the highly efficient power amplification cell adds peak clipping and self-adaptive digital predistortion processes to the digital signal process cell by a symmetric Doherty technology so that the whole device achieves qualified linearity while improving the efficiency. The invention has the beneficial effects of effectively guaranteeing that the linearity index meets the requirements and solving the contradiction of linearity and efficiency of the radio frequency when improving the efficiency of the whole system, and also tracking the power amplification linearity feature changes due to the changes of work conditions and work environments, such as device aging, temperature change and the like, so as to always keep great linear effect.

The present invention relates to methods of obtaining genetic competence in non-competent Bacillus cells for their transformation with exogenous DNA.

The invention relates to a method for preparing natural 4-vinyl guaiacol by biological transformation. According to the method disclosed by the invention, Bacillussp. HY3CCTCCM2012247 is taken as a biocatalyst, natural ferulic acid is taken as a substrate, and the natural 4-vinyl guaiacol is prepared by microbial transformation. The production process comprises the following steps of: (1) performing slant culture; (2) preparing a seed culture solution; (3) culturing transformation cells; and (4) transforming. The method disclosed by the invention belongs to the technical field of biology, thenatural 4-vinyl guaiacol can be produced with high transformation rate by adopting the method, and the method can be used in the fields of tobacco, wine, foods and the like and further has great application prospects.

Methods and DNA constructs are provided for detection and manipulation of a target eukaryotic gene whose expression is restricted to certain tissues or specialized cell types. The methods include transforming a cell with a first indicator component under the control of a promoter selected for its restricted expression in a particular cell or tissue. The cell is also transformed with a gene trap vector encoding a second indicator component. The cell is allowed to differentiate to produce specialized cell or tissue which is monitored for expression of both the first and second indicator components, thereby detecting a gene into which the trap vector has integrated which is expressed in the same cell or tissue type as the selected promoter.

This invention describes a new alpha-CTX, its encoding polynucleotide, the construction units and transformation cells having this encoding polynucleotide, and the reconstruction process for producing the said alpha-CTX. This invention also describes the artificial synthesis and applications of the said alpha-CTX.

Methods of inserting genes into defined locations in the chromosomal DNA of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest. The invention also relates to meganucleases, vectors and engineered cell lines necessary for performing the methods, to cell lines resulting from the application of the methods, and use of the cell lines to produce protein products of interest.

The present invention provides recombinant viruses which replicate the viral genome selectively in response to the intracellular conditions of the target cell through the use a pathway-responsive promoter which substantially inhibits viral replication in the host cell based on the phenotypic or genotypic of the infected cell. In the target cell, the promoter element of the pathway-responsive promoter is inactive and thus the virus is permitted to replicate. This results in: (1) killing the cells by natural lytic nature of the virus, and / or (2) provides a therapeutic dose of a transgene product (amplified in comparison to replication incompetent vectors) to the target cell, and (3) producing a localized concentration of the virus facilitating the infection of surrounding cells to the recombinant virus. The invention further provides therapeutic and diagnostic methods of use of the vectors, pharmaceutical formulations comprising the vectors, methods of making the vectors and transformed cells comprising the vectors.

The subject invention pertains to a method of rapidly transforming a plant with a gene of interest. The method of the current invention comprises the steps of a) preparing a bacterial culture, wherein the bacterial culture comprises a vector containing the gene of interest, b) producing a plant cell suspension culture from the plant, c) contacting the plant cell suspension culture with the bacterial culture to produce a plant cell transformed with the gene of interest from the plant cell suspension culture, and e) producing a plant from the plant cell transformed with the gene of interest. The method of the current invention can be designed for a high throughput transformation and screening of a plant with a plurality of genes of interest and screening the plants to identify and obtain plants having desirable characteristics.

The present invention provides one new kind dahlia Streptoverticillium resisting receptor protein (S1Vc1) expressed in Solanum lycopersicoides Dun. and its coding sequence and application in improving plant disease resistance, especially in resisting verticillium wilt. The present invention relates to fusion gene constituent and new recombinant expression vector carrying the constituent, as well as transgenic plant generated via transforming plant cell with the expression vector and its subsequent generation, including plant seed and plant tissue. The present invention expresses the said gene in plant to obtain transgenic plant with strengthened resistance to plant diseases, especially verticillium wilt.

The invention belongs to the field of plant genetic engineering and transgenic breeding, and provides a method for culturing and identifying a recombinant vector containing a CmWRKY48 gene, a transformed cell and CmWRKY48 trans-genetic chrysanthemum morifolium and application thereof. The method for culturing CmWRKY48 trans-genetic chrysanthemum morifolium comprises the following steps: (1) cloning a CmWRKY48 gene of chrysanthemum morifolium; (2) constructing a plant expression vector pMDC43-CmWRKY48; (3) transforming the chrysanthemum morifolium by an agrobacterium EHA105 mediated leaf disc method. PCR (Polymerase Chain Reaction) and quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detections on the transformed plant prove that the CmWRKY48 gene is integrated to the DNA of the genome of the transgentic plant and performs transcription. An anti-aphis detection is performed on the transgenetic plant, and the anti-aphis capability of the transgenetic plant is greatly improved, so that a novel and practical method is provided to breeding of stress tolerance variety of chrysanthemum morifolium by utilizing a gene engineering technology, and the progress of chrysanthemum morifolium biotechnological breeding can be effectively promoted.

The invention belongs to the technical fields of molecular biology and genetic engineering, relates to a new Late Embryogenesis Abundant protein ('LEA protein' for short) which is expressed in the Jatropha curcas, and an encoding sequence and application in improving drought tolerance of plants, and relates to fusion gene structures containing the genes, new recombined expression vectors carrying the fusion gene structures, and transformation plant cells, and transgenic plants of the genes generated by the transformation cells and offsprings thereof, which comprise plant seeds and plant tissues. By introducing the genes into plant hosts, drought stress resistance enhanced transgenic plants (crops) can be obtained. The genes have important theoretical and practical significances for culturing new breeds of drought resistance enhanced crops, and can be applied to breeding and identifying plant resistance breeds needed by farming and animal husbandry and ecological environment management.