11results about How to "High optical purity" patented technology

This invention provides an imine reductase mutant, its preparation method, and its application in the catalytic preparation of dextromethorphan intermediates. The imine reductase mutant is an imine reductase with an amino acid mutation, comprising the amino acid sequence shown in SEQ ID NO:1. The amino acid mutation type includes any one or a combination of at least two of I122T, D212A, or G228R. By introducing a mutation into the imine reductase, this invention significantly improves the enzyme's activity and stereoselectivity, enabling the high-yield synthesis of the dextromethorphan intermediate (S)-1-(4-methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline under mild conditions, greatly reducing production costs and making it suitable for industrial production.

The invention discloses a chiral alpha-alkenyl substituted carboxylic ester compound and an asymmetric preparation method thereof, and the chiral alpha-alkenyl substituted carboxylic ester compound is an optically active compound with a structure as shown in the following formula I, and comprises a stereoisomer with the same chemical general formula, in the formula, * represents a chiral carbon atom; ar is an aromatic ring or a substituted aromatic ring; r1, R2, R3, R4 and R5 are respectively and independently selected from hydrogen, halogen, alkyl or alkoxy of C1-10, aryl and trifluoromethyl; r6 is independently selected from H, C1-10 alkyl or alkoxy, aryl or substituted aryl, and a substituent group of the substituted aryl is selected from halogen and C1-10 alkyl. According to the invention, the chiral alpha-alkenyl substituted carboxylic ester compound is accurately and rapidly synthesized with high yield, high stereoselectivity and gram-level scale. The preparation method is simple, and has biomedical practicability and industrial application prospects.

The invention discloses a production process for blue glass, and relates to the technical field of blue glass production. The process comprises the following steps: S1, raw material pretreatment: respectively grinding and drying a basic glass raw material, a coloring agent and a functional additive, and then mixing in sequence; s2, melting the mixture in a primary melting furnace in a reducing atmosphere, and adding a reducing agent; s3, transferring the primarily molten glass liquid into a main furnace, and stirring and clarifying in a nitrogen-oxygen mixed protective gas environment; s4, cooling the clarified glass liquid, and continuously stirring and homogenizing; according to the preparation method, the basic raw materials, the coloring agent and the functional additive are accurately proportioned, and control steps of staged mixing, reducing atmosphere melting, multi-stage clarification and homogenization, float forming and the like are combined, so that synergistic improvement of high optical purity and excellent mechanical performance is realized. In the process, the purity, the granularity, the charging sequence and the thermal regulation of the raw materials are strictly controlled, so that the component uniformity and the product consistency are guaranteed.

The application discloses a lysine cyclodeaminase, mutants, recombinant genetically engineered bacteria and application thereof. The amino acid sequence of the lysine cyclodeaminase is shown as SEQ ID NO. 1. The lysine cyclodeaminase mutants include: mutants I61V, I94V, I233V and A235S obtained by taking the lysine cyclodeaminase with the amino acid sequence shown as SEQ ID NO. 1 as a template and respectively mutating the 61st, 94th, 233rd and 235th positions, a mutant I233V / A235S obtained by iterative saturation mutation of the 233rd and 235th positions, and mutants I94V / I233V / A235S and I61V / I94V / I233V / A235S obtained by hot combination mutation. The lysine cyclodeaminase and the mutants thereof obtained according to the application have high enantiomeric selectivity and activity, and can catalyze L-lysine to generate optically pure L-piperidinecarboxylic acid.

The invention provides a preparation method of ((1R, 3S)-3-hydroxycyclopentyl) tert-butyl carbamate, which comprises the following steps: (1) mixing (R)-3-oxocyclopentane carboxylic acid with alkali, diphenyl azide phosphate and tert-butyl alcohol for reaction to obtain an intermediate; and (2) mixing the intermediate with a chiral catalyst and borane for reaction to obtain ((1R, 3S)-3-hydroxycyclopentyl) tert-butyl carbamate. The preparation method provided by the invention has the advantages of cheap and easily available raw materials, simple process operation, safe and controllable production and high optical purity of the target product, and can meet the requirements of industrial production.

The invention discloses a method for preparing fenerenone from a fenerenone raceme and refining and crystallizing an intermediate. Chirally substituted tartaric acid ester is adopted to prepare an intermediate, namely, a non-nepetenone diastereomer salt III, and then the non-nepetenone diastereomer salt III is subjected to alkali dissociation to prepare the non-nepetenone (IIa). According to the invention, by adding a refining and crystallizing method of the intermediate fenerenone diastereomer salt (III), the ee value obtained by the prepared intermediate fenerenone diastereomer salt (III) is higher than 99.5%, and the e.e value in a fenerenone bulk drug further prepared on the basis is basically 100%; a resolving agent tartrate is not detected in the free fenerenone enantiomer, product loss and three-waste generation caused by multiple dissociation are avoided, the production and manufacturing cost is greatly reduced, the product prepared by the method is high in yield and low in three-waste pollution, the preparation process is extremely simple to operate, the reaction condition is mild, the production cost is saved, and the method is suitable for industrial production. The method is suitable for industrial production.

The present application relates to the technical field of delta-lactone synthesis, in particular to a high-purity delta-lactone synthesis method, comprising the following steps: using potassium carbonate to catalyze Michael addition reaction of the active methylene functional group of dimethyl malonate and the alpha, beta-alkenyl functional group of 1-octene-3-ketone to generate intermediate product I; intermediate product I generates decarboxylation reaction under the catalysis of boric acid to generate intermediate product II; chiral ligand supported nickel catalyst catalyzes asymmetric hydrogenation reaction of the delta-carbonyl functional group of intermediate product II to generate chiral delta-hydroxyl functional group, and ester exchange reaction of the chiral delta-hydroxyl functional group and the ester functional group of intermediate product II to generate chiral delta-decalactone. The present application designs and synthesizes four chiral ligand supported nickel catalysts on one hand, and designs a new route capable of synthesizing chiral delta-decalactone with high optical purity on the other hand.

The application discloses a synthesis method of chiral amino alcohol compounds. The synthesis method of the chiral amino alcohol compounds comprises the following steps: in the presence of a carbonyl reductase and a coenzyme, a carbonyl compound shown as formula II is subjected to a reduction reaction shown as formula I in an organic solvent and a buffer solution to obtain a conversion solution containing the chiral amino alcohol compound shown as formula I. 1 The application uses a racemic aziridine chiral compound as a starting material, and through enzyme reaction of recombined carbonyl reductase, a single configuration chiral amino alcohol is efficiently synthesized, an important intermediate with a high de value is obtained, and production cost is reduced.

This invention provides a novel crystalline form of phenelzine optically active intermediate IIa and its preparation method. The powder X-ray diffraction pattern of this novel crystalline form has the following characteristic diffraction angles: 8.89°, 12.78°, 19.33°, 21.43°, 22.05°, 26.77°, and 27.01°, with an error range of ±0.2° for the diffraction angle 2θ values. This invention also provides a method for preparing this novel crystalline form and its pharmaceutical applications, including its use in the preparation of phenelzine, and even its use as a chiral resolving agent in the preparation of phenelzine.

This invention discloses an alcohol dehydrogenase derived from *Rhodotorula toruloides* and its application in the asymmetric reduction of 1-(2-chlorophenyl)-2-(1,2,3,4-tetrazol-2-yl)ethane-1-one to (R)-1-(2-chlorophenyl)-2-(1,2,3,4-tetrazol-2-yl)ethane-1-ol. The alcohol dehydrogenase can catalyze substrate conversion reactions at concentrations greater than 400 g / L, with a conversion rate >99.9% and a product ee value >99.5%. The reaction rate is fast, easily controlled, and the catalytic level is significantly improved compared to previous reports, demonstrating great potential for industrial application.

The application discloses an organic quaternary phosphonium salt microcrystal flexible X-ray scintillation screen and a preparation method and application thereof, and belongs to the technical field of organic photoelectric functional materials. The organic radiation luminescent material is prepared from triphenylphosphine and iodomethane as raw materials through a quaternary phosphonium reaction to obtain methyltriphenylphosphonium iodide crystals, and then the methyltriphenylphosphonium iodide crystals are ground, sieved, compounded with a PVA aqueous solution and drop-coated and dried to obtain the flexible X-ray scintillation screen. The preparation method has the advantages of mild reaction conditions, simple synthesis, cheap raw materials and easy product purification, and provides a new idea for preparing an organic scintillator. The PVA aqueous solution is doped with methyltriphenylphosphonium iodide microcrystals to prepare the scintillation screen, the prepared scintillation screen has the advantages of bright radiation luminescence, high imaging resolution, low detection limit, bendability and curlability, and successfully realizes static, curved surface and dynamic X-ray imaging.