33 results about "Candida glabrata" patented technology

The invention belongs to the technical field of antibacterial active microorganisms, and particularly relates to a strain of diffusion carbon pad bacteria LUCF 4343-3, a bacterial agent and application of the bacterial agent in preparation of an antibacterial preparation. According to the invention, an endophytic fungus strain with remarkable antibacterial potential is successfully separated from Parmotrema, and the endophytic fungus strain is identified as diffusion carbon pad fungus LUCF 4343-3. Experiments show that an ethyl acetate extract of the strain shows a good inhibition effect on various clinical common pathogenic bacteria, including gram-positive bacteria (staphylococcus aureus and enterococcus faecalis), gram-negative bacteria (pseudomonas aeruginosa), fungi (candida albicans and candida glabrata) and the like; the discovery provides an important basis for the application of the strain in the development of novel broad-spectrum antibacterial drugs.

The invention discloses application of tannic acid combined triazole medicines in preparation of medicines for treating diseases caused by fungi, and belongs to the technical field of antifungal. A series of drug sensitive tests show that tannic acid lower than 3 [mu] g / ml has no influence on the activity of human vaginal epithelial cells, and after the tannic acid is combined with fluconazole, the tannic acid can significantly inhibit the growth activity of drug-resistant candida (including drug-resistant candida albicans and drug-resistant candida glabrata). In view of the natural property, wide source and low toxicity of tannic acid, the invention provides a new antifungal treatment strategy, and the tannic acid has important clinical application value for treating infection (such as vaginal candidiasis) caused by drug-resistant fungi, especially under the condition that the existing antifungal drugs are poor in effect. Therefore, the tannic acid combined triazole medicine can be used for preparing the medicine for treating diseases caused by fungi.

The invention relates to the technical field of biological medicine, in particular to application of a combination of a Cd1 and Mdr1 double-target inhibitor and an azole drug in preparation of an antifungal drug. Based on the fact that the compound as shown in the formula (I) can be used as a CCd1 and Mdr1 double-target inhibitor, the inhibition effect of the compound on candida albicans, candida auricula, candida glabrata, candida tropicalis, trichophyton rubrum and trichophyton indicus when the compound is combined with azole drugs is further evaluated, and the result shows that the compound as shown in the formula (I) has no obvious antibacterial activity on various fungi, and can be used for preparing a medicine for treating various fungi. The drug resistance of CCd1 and Mdr1 overexpression drug-resistant fungi can be reversed, the activity of Candida albicans, Candida auricula, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indicus can be synergistically inhibited when the drug is combined with azole drugs, the treatment dosage of the azole drugs is effectively reduced, and the drug toxicity is small. Therefore, the compound shown in the formula (I) can improve the antifungal effect of azole drugs and reduce the drug resistance of the azole drugs.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The present invention discloses a mutant dual-enzyme catalyst, application and (S)-Bosin synthesis method, relating to the field of bioengineering technology, the present invention is based on the methylglyoxal reductase GRE2 (A) of Candida glabrata and the phosphite dehydrogenase PTDH of methylotrophic bacteria as sources for transformation, and its mutants are screened, the key residues affecting the enzymatic properties of the initial methylglyoxal reductase and phosphite dehydrogenase are determined, and methylglyoxal reductase mutants and phosphite dehydrogenase mutants with high activity are obtained. The present invention utilizes the two most efficient mutants to catalyze the reaction of preparing (S)-Bosin from β-acetone xyloside in a 22T reaction system, with good catalytic effect, overall catalytic efficiency is increased by about 43%, and cost is reduced by about 8%.

The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.

The invention relates to the technical field of pathogenic microorganism infection detection, and provides a respiratory flora marker for assisting in predicting chlamydia psittaci infection and application of the respiratory flora marker. The respiratory tract flora markers comprise Candida glabrata, Lactobacillus paracasei, and Lactobacillus rhamnosus, and the respiratory tract flora markers comprise Candida glabrata, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus paracasei, and Lactobacillus rhamnosus. The respiratory flora marker for aided prediction of chlamydia psittaci infection is applied to preparation of a kit for aided diagnosis of severe pneumonia caused by chlamydia psittaci infection, has high sensitivity and high specificity, reduces misdiagnosis and missed diagnosis, can effectively evaluate the risk of severe illness of a patient suffering from chlamydia psittaci infection, and has a good application prospect in the treatment of severe pneumonia caused by chlamydia psittaci infection. Therefore, medical intervention can be carried out as soon as possible, and great clinical application value is achieved.

The invention discloses an inffumafungin analogue AsepticFungin with antifungal activity as well as preparation engineering bacteria and application of the inffumafungin analogue AsepticFungin. A series of AsepticFungin triterpenoid glycoside products with novel structures are obtained through fermentation and separation by virtue of a microbial heterologous expression platform, and AsepticFungin compounds with antifungal activity are screened out; the obtained compound has specificity in antifungal activity and can specifically inhibit growth of candida glabrata, the activity of AseptuFunginB is better, 90% of candida glabrata can be killed under the dosage of 1 microgram / L, and the compound can be used as a candidate drug for later antifungal activity.

This invention provides a method for inhibiting the growth of a pathogenic mold by contacting the pathogenic mold with a synergistically effective amount of an NK1 antagonisfand an antifungal agent. This invention also provides a method for inhibiting the growth of a mammalian fungal pathogen by contacting the mammalian fungal pathogen with a synergistically effective amount of posaconazole and rolapitant. In some aspects, the mammalian fungal pathogen is a species of Candida (e.g., C. glabrata, C. auris, C. albicans, C. dubliniensis, or C. parapsilosis). Aspergillus, Cryptococcus, Mucor, orRhizopus. In addition, A surface disinfectant comprising a synergistic amount of an NK1 antagonist and an antifungal agent in admixture with a carrier or excipient.

The invention belongs to the technical field of information, and discloses a copper inducible plasmid which comprises a yeast shuttle vector, a Candida glabrata-derived CEN / ARS element loaded on the yeast shuttle vector, a copper ion inducible promoter loaded on the yeast shuttle vector and a target gene, the target gene is connected to the downstream of the copper ion induced promoter. After being transfected into candida glabrata, the plasmid can show the following advantages: the plasmid is stable, the stability of host bacteria cannot be influenced on the basis of controllable expression of the dosage of an inducer, and simultaneous expression of various target proteins can be realized on the basis of loads of different target genes of the plasmid. Meanwhile, the invention also discloses an inducible expression method of the target gene.

This invention discloses a plant essential oil composition with anti-Candida activity and its preparation method, which consists of a heavy oil component of Sichuan pepper and a light oil component of dried ginger in a volume ratio of 1:8 to 2:1. Research in this invention has found that, after specific volume ratios, particularly when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 1:8, it exhibits a synergistic effect against Candida albicans, Candida tropicalis, and Candida glabrata; when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 2:1, it exhibits a very good synergistic effect against Candida parapsilosis. This invention allows the heavy oil component of Sichuan pepper and the light oil component of dried ginger to be formulated into an anti-Candida drug in a specific volume ratio.

The present application relates to a protein feed containing a composite fermentation composition, and belongs to the technical field of microbial fermentation and feed.The protein feed comprises fermentation strains and fermentation substrates; the fermentation strains comprise Rhodopseudomonas palustris with a preservation number of CGMCC NO.17021 and Candida glabrata with a preservation number of CCTCC NO:M2015664; and the fermentation substrates comprise cottonseed meal powder, rapeseed meal powder, distiller's grains powder and citrus dreg powder.The protein feed containing the composite fermentation composition has a high feed utilization rate and can improve the immunity of the animals fed with the protein feed.

The present application relates to the technical field of biological medicine, in particular to the application of Cdr1 and Mdr1 double-target inhibitor combined with azole drugs in the preparation of antifungal drugs. Based on the compound shown in formula (I) can be used as Cdr1 and Mdr1 double-target inhibitor, the inhibitory effect of the compound on Candida albicans, Candida auris, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indiana combined with azole drugs was further evaluated. The results show that the compound shown in formula (I) itself has no obvious antibacterial activity on various fungi, but can reverse the drug resistance of Cdr1 and Mdr1 overexpression drug-resistant fungi. The combination of the compound with azole drugs can synergistically inhibit the activity of Candida albicans, Candida auris, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indiana, effectively reduce the therapeutic dose of azole drugs, and the drug toxicity is small. Therefore, the compound shown in formula (I) can improve the antifungal effect of azole drugs and reduce the drug resistance of azole drugs.

The invention relates to the technical field of biological medicine, in particular to an antifungal pharmaceutical composition based on a (1, 3)-beta-D-glucan synthase inhibitor. In-vitro experiment results show that the antibacterial peptide LL-37 and (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) are combined for use to achieve the effect of synergistically inhibiting candida albicans, candida auricula, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei, so that the antibacterial peptide LL-37 can be combined with the (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) to achieve the effect of inhibiting the (1, 3)-beta-D-glucan synthase inhibitors). And 3) preparing the antifungal drug by combining the 1, 2, 4, 5, 6, 7, 8, 8, 9, 10- However, the antibacterial peptide LL-37 is easily degraded by in-vivo protease, so that the expression of the antibacterial peptide LL-37 in an organism is up-regulated by adopting an antibacterial peptide LL-37 expression accelerant, and the antibacterial peptide LL-37 and a (1, 3)-beta-D-glucan synthetase inhibitor are synergistically antibacterial in the organism.

The present invention belongs to the field of antibacterial active microorganism technology, and specifically relates to a strain of Citrus citriodora LCUF 4067-1, a bacterial agent, and its application in the field of broad-spectrum antibacterial. The present invention screened an endophytic fungus from Prunus armeniaca and named Citrus citriodora LCUF 4067-1. Black spore orange )LCUF 4067‑1 ， The ethyl acetate extract of this fungus shows inhibitory activity against a variety of harmful microorganisms such as Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida glabrata and Candida albicans, and is expected to be used in the development of new antibiotics or antibacterial preparations to achieve broad-spectrum antibacterial effects.

The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.

This invention discloses a broad-spectrum antibacterial composition derived from Syzygium sima (Syzygium sima) and its preparation method, relating to the field of biomedical technology. The composition comprises an active ingredient and pharmaceutically acceptable excipients. The active ingredient includes ursane-type triterpenoids or their pharmaceutically acceptable salts, esters, or prodrugs. The active ingredient provided by this invention exhibits potent inhibitory activity against a variety of clinically common pathogenic fungi, particularly showing significant antibacterial effects against Candida albicans, Cryptococcus neoformans, Candida glabrata, Aspergillus fumigatus, and Aspergillus niger. This compound is a natural product with an ursane-type triterpenoid skeleton, featuring a structure with α-L-rhamnose linked at C-3 and a free carboxyl group retained at C-28, overcoming the shortcomings of some existing plant-derived antibacterial agents, such as narrow antibacterial spectrum and poor efficacy against specific fungi.

This invention discloses a strain of Candida smoothiae ( Candida metapsilosis This invention relates to DL-GZRT3 and its applications, belonging to the fields of microbial biotechnology or food biotechnology. The DL-GZRT3 strain of this invention grows well under conditions of 15% NaCl concentration, pH 2, and 6% ethanol concentration. It is tolerant of high salt, acid, and ethanol; it can tolerate the gastrointestinal environment, exhibiting excellent probiotic properties and good safety. The DL-GZRT3 strain possesses good antioxidant properties, with a DPPH scavenging rate of 82.93% and an ABTS scavenging rate of 66.95%, and can produce high concentrations of esters and alcohols during fermentation. Therefore, *Candida glabrata* strain DL-GZRT3 can ensure the safety and flavor quality of fermented foods, shorten the fermentation cycle, and significantly improve the nutritional value of fermented foods.

The invention relates to the technical field of bioengineering, in particular to a ketoreductase mutant as well as a preparation method and application thereof. The ketoreductase cgKR1 derived from candida glabrata is mutated to obtain a ketoreductase mutant, and the ketoreductase mutant is applied to preparation of (R)-3-chloro-1-phenyl-propanol by reducing 3-chloropropiophenone, and compared with wild type ketoreductase cgKR1, the yield of the (R)-3-chloro-1-phenyl-propanol is increased, and the yield of the (R)-3-chloro-1-phenyl-propanol is increased. The ketoreductase mutant provided by the invention has higher enzyme activity and stereoselectivity on the reduction reaction of 3-chloropropiophenone, the isomer purity of a reaction product is greatly improved, and industrial production requirements can be better met.

The invention belongs to the technical field of in-vitro molecular detection of pathogenic microorganisms, and particularly provides a primer probe group and a reagent for multiple fluorescent PCR (Polymerase Chain Reaction) and / or multiple quantitative digital PCR detection of five candida. The primer probe group comprises specific primer pairs and corresponding probes aiming at candida albicans, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei. Based on the unified combination, two compatible detection paths are established: five-target synchronous qualitative detection can be realized by adopting a multiple fluorescent PCR (Polymerase Chain Reaction) method, and five-target absolute quantitative analysis can also be realized by adopting a multiple quantitative digital PCR method; the two methods can be applied independently or jointly. Through verification, the primer probe group has high specificity; the sensitivity is excellent, and DNA extracted from a clinical sample can be directly detected. The method is simple in detection process and short in time consumption, and an efficient and reliable molecular detection solution is provided for early differential diagnosis and precise medication of clinical invasive candidiasis.

The application belongs to the field of biology, discloses a Candida glabrata, the classification name of the Candida glabrata is Nakaseomyces glabratus YX2024, the preservation unit of the Candida glabrata is China Center for Type Culture Collection, the preservation time is December 23, 2024, the preservation number is CCTCC NO: M 20242881, and the preservation address is China.Wuhan.Wuhan University.The enzyme activity of the lipase produced by the bacteria is significantly higher than that of other strains screened in the same batch, and the bacteria have excellent acid resistance, gastric juice resistance and intestinal juice resistance characteristics.Meanwhile, the application also discloses the use based on the Candida glabrata.

The invention discloses a histioplasma capsulatum double-antibody sandwich enzyme-linked immunosorbent assay method, a reagent and application. The invention relates to the technical field of ELISA (enzyme-linked immunosorbent assay). According to the present invention, the Protein A / G magnetic beads are used to directionally fix the antibody and ultrasonically assist the antigen release, such that the antigen binding site is completely exposed, and the hidden antigen is released, such that the test sensitivity is significantly improved, the sensitivity can be improved by 10 times, and the detection limit is reduced to the lower level (such as 1 ng / mL or even 0.1 ng / mL). Due to the use of the block copolymer / zwitterionic surfactant, non-specific binding is effectively blocked, background signals are reduced, and the test specificity is improved; the method comprises the following steps: S1, antibody coating: diluting a captured antibody to a working concentration (generally 1-10 [mu] g / mL) by using a coating buffer solution, and adding into an ELISA plate hole (100 [mu] L / hole); carrying out overnight incubation at 4 DEG C (or at 37 DEG C for 2h) after plate sealing; and discarding the liquid, and washing with PBST for 3 times (300 [mu] L / hole, and drying after standing for 1 min each time). Protein A / G magnetic beads are used for directionally fixing the antibody through an Fc segment.

The present application provides a method and a kit for detecting blood stream infection pathogens and application. Specifically, the present application provides a primer probe combination for detecting blood stream infection pathogens, which comprises: (a) upper and lower primers and probes for detecting Candida as shown in SEQ ID NO: 4~6; (b) upper and lower primers and probes for detecting Candida glabrata as shown in SEQ ID NO: 1~3; (c) upper and lower primers and probes for detecting Candida tropicalis as shown in SEQ ID NO: 7~9; and (d) upper and lower primers and probes for detecting Cryptococcus as shown in SEQ ID NO: 10~12. The primer probe combination of the present application can be used to achieve rapid, high sensitivity and high specificity detection of blood stream infection pathogens.

The present invention belongs to the field of antibacterial active microorganism technology, and specifically relates to a diffusion carbon mat fungus LUCF 4343-3, a bacterial agent and its application in the preparation of antibacterial preparations. Parmotrema ) were successfully isolated from a strain of endophytic fungus with significant antimicrobial potential and identified as LUCF 4343‑3. Experiments showed that the ethyl acetate extract of this strain exhibited potent inhibitory effects against a variety of common clinical pathogens, including Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa), and fungi (Candida albicans and Candida glabrata). These findings provide important evidence for the application of this strain in the development of new broad-spectrum antimicrobial drugs.

The invention belongs to the technical field of antibacterial active microorganisms, and particularly relates to a melanospora citrina strain LCUF 4067-1, a fungicide and application of the melanospora citrina strain LCUF 4067-1 in the field of broad-spectrum bacteriostasis. According to the invention, an endophytic fungus is obtained by screening from large-leaf plum, the endophytic fungus is named as nigrospora citrina LCUF 4067-1, and an ethyl acetate extract of the fungus shows inhibitory activity on a plurality of harmful microorganisms such as enterococcus faecalis, staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida glabrata and candida albicans; the compound is expected to be applied to development of novel antibiotics or antibacterial preparations, and the broad-spectrum antibacterial effect is achieved.

The invention provides a microbial agent for efficiently degrading kitchen waste. The microbial agent comprises a specific strain and a carrier. According to the microbial agent, efficient degradation of kitchen waste is achieved through the synergistic effect of a traditional strain and a thermophilic strain on a carrier; wherein the traditional strains comprise brevibacterium aureum, bacillus licheniformis, bacillus subtilis, bacillus amyloliquefaciens, vegetable bacillus, bacillus tequilensis, stenotrophomonas maltophilia, candida glabrata and pediococcus acidilactici; the thermophilic strains comprise novel bacillus thermophilus, bacillus pumilus, bacillus thermophilus deamination bacillus and bacillus megatherium. The microbial agent is stable in degradation effect, simple and convenient in preparation process, low in cost and free of environmental pollution.

The present disclosure provides compositions and methods for treating fungal infections. Disclosed here are proteins (Ait1p) that bind to TORC1 and regulate cell growth in yeast cells. These proteins and agents that bind to them may be effective in treating infection, particularly those caused by yeast, such as Candida glabrata.

The application relates to the technical field of medicines, and relates to an antifungal fatty acid inhibitor, in particular to application of D-646 in preparation of an antifungal medicine; the fungus inhibited by the antifungal medicine does not include Candida glabrata. The application provides a new scheme for prevention and treatment of fungi.

The application discloses a heavy Zanthoxylum bungeanum essential oil component with antibacterial activity and a preparation method thereof. The Zanthoxylum bungeanum essential oil is deeply researched, and the Zanthoxylum bungeanum essential oil is separated into a light Zanthoxylum bungeanum essential oil component and the heavy Zanthoxylum bungeanum essential oil component through a molecular distillation instrument. The antibacterial and antifungal experimental results show that the heavy Zanthoxylum bungeanum essential oil component obtained through distillation refining has better bactericidal activity on Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, Staphylococcus aureus and corynebacterium striatum compared with the Zanthoxylum bungeanum essential oil and the light Zanthoxylum bungeanum essential oil component, and has better bactericidal activity on Candida tropicalis, Candida krusei, Candida glabrata and white Candida, and a good technical effect is achieved.