48results about How to "High biological activity" patented technology

An in-situ biologic repairing method for the soil polluted by petroleum features that after the fungus-bacteria mixed liquid, soil improver, organic fertilizer and inorganic nutrients are applied to the polluted soil, the soil is irrigated, ploughed, and covered by plastic film.

Biologically active molecules are produced from their biologically inactive precursors by mutation of their native cleavage site to a site which is capable of being cleaved by a protease, and cleavage of the mutated molecule to yield a biologically active molecule.

The invention specifically relates to application of a halogenated indole and derivatives thereof as a marine antifouling agent, belonging to the technical field of marine antifouling paints. The invention provides application of one or more selected from the group consisting of halogenated indole and derivatives thereof as the marine antifouling agent. According to the invention, the halogenated indole compounds can inhibit attachment of marine stained microbes, marine algae and large-scale marine stained animals and presents high-efficiency and wide-spectrum antifouling activity. The halogenated indole compounds extensively exist in marine organisms, are easy to biodegrade and have low toxicity and no pollution; a marine antifouling agent prepared from the halogenated indole compounds does not contain heavy metals and causes no harm to the environment. Moreover, an artificial synthetic method for the halogenated indole compounds is simple, which facilitates application of the halogenated indole compounds in marine antifouling paints.

The present invention relates to the technical field of oleanolic acid drugs and provides a drug sustained release agent based on oleanolic acid and a preparation method thereof. The drug sustained release agent based on the oleanolic acid is applied to drugs with the oleanolic acid as a main drug component and is prepared from the components including a drug carrier, a hydrophilic gel material, a erodible matrix material and an insoluble matrix material, wherein the drug carrier is β-cyclodextrin-chitosan composites, wherein the oleanolic acid is from a plant raw material, and a host-guest inclusion complex is composed of the main drug component and the drug carrier according to the mass ratio of 0.1:0.1-0.1:5. A preparation method comprises the following steps: preparing the inclusion complex, mixing auxiliaries, carrying out compression moulding and the like. The drug sustained release agent based on oleanolic acid has the characteristics of stable drug concentration, high biological activity, good drug solubility and long acting effect.

Cyclic polyaza chelators that possess high affinity and specificity for first transition series metal cations exhibit an unanticipated improvement in biological activity when administered as complexes with cations of the alkaline earth metals, Ca(II) and Mg(II), most notably Ca(II). By virtue of this improvement, these complexes are particularly effective in the treatment of pathological conditions, including ischemia and ischemia-reperfusion injury.

The invention discloses a purification method for recombinant human interferon beta-1a. The method comprises three steps: affinity chromatography, strong anion exchange chromatography and weak cation exchange chromatography. Blue dyeing affinity chromatography and interferon beta antibody affinity chromatography are adopted during affinity chromatography, Q-sepharose chromatographic column is adopted during strong anion exchange chromatography, and CM-sepharose chromatographic column is adopted during weak cation exchange chromatography. With the method, reverse phase chromatography and metal-chelating chromatography steps influencing protein activity are abandoned, two-time ionic exchange chromatography is adopted, thus obtaining the recombinant human interferon beta-1a with high purity and good bioactivity.

The invention discloses probiotic fruit juice beverage which is prepared from fermented compound berry juice, dietary fiber, fish gelatin protein peptide, stachyose, elastin peptide, whitening peptide and liver protecting peptide. The probiotic fruit juice beverage has the functions of whitening and brightening skin, making skin elastic, moisturizing skin, removing wrinkle, fading patch, resisting oxidation, delaying senescence, nourishing liver and protecting liver. The invention further discloses a preparation method of the probiotic fruit juice beverage. The nutrient absorption rate is improved.

The invention discloses a bionic bone repair material having good machinery performance and biological activity and a preparation method thereof. The preparation method comprises the following steps: 1) using a rapid moulding technology for performing three-dimensional printing on a large aperture PCL rack to obtain a primary structure; 2) activating the surface of the PCL rack having the primary structure; 3) employing collagen network for performing three-dimensional function activation on the PCL rack having the primary structure to obtain a Col-PCL rack having a secondary structure; and 4) employing nano hydroxyapatite particles for bionic mineralization on the Col-PCL rack to obtain an AP-Col-PCL rack having a third structure; The product combines a rapid moulding technology and a biomaterial three-dimensional modification technology, constructs the bionic bone repair material AP-Col-PCL having good machinery performance and biological activity which, and can be used for bone defect research and clinical treatment.

The invention discloses tandem duck Alpha and Nu interferon genes and a preparation method and application thereof and belongs to gene fusion expression in the biotechnical field. A nucleotide sequence of the tandem duck Alpha and Nu interferon genes is shown as in SEQ ID No. 1. The invention also provides the preparation method of the tandem duck Alpha and Nu interferon gene, a prokaryotic expression plasmid Alpha and Nu interferon prokaryotic expression plasmid pET32a-IFNAlpha-linker-IFNNuexpressing the tandem duck Alpha and Nu interferon gene and its preparation method, and application of a fusion protein expressed by the tandem duck Alpha and Nu interferon gene in the preparation of duck antiviral drugs. Recombinant duck Alpha and Nu interferon prokaryotic expression plasmid pET32a-IFNAlpha-linker-IFNNu is established successfully herein, the duck IFN-Alpha and IFN-Nu genes are tandemly expressed on an Escherichia coli prokaryotic expression system, and it is also possible to ensure that the two interferon genes are expressed in a ratio of 1:1.

The invention discloses a totarol antibacterial nano hydrogel with a whey protein as a matrix and a preparation method thereof, and belongs to the bacteriostatic gel biological technical field. Preparation raw materials comprise a whey protein gel, totarol and absolute ethyl alcohol. The preparation method adopts a sol-gel technique and comprises the steps of whey protein gel preparation, totarol embedding, ultrasonic treatment and standing and sterilization. According to the antibacterial nano hydrogel formula, a novel food-borne bacteriostatic agent is achieved. At the same time, the antibacterial nano hydrogel has the characteristics and advantages of the whey protein and the totarol. A whey protein polymer plays dispersion stabilizer, gel matrix and multiple roles in the antibacterial nano hydrogel system, no any crosslinking agents and initiators are added, toxic effects on a human body cannot be produced, and the use is safer and more effective; the preparation method has the advantages of simple equipment and technological process for producing the antibacterial nano hydrogel, mild conditions, low cost and easy popularization, and is suitable for industrialized production.

The invention discloses a traditional Chinese medicine polysaccharide immunoenhancer for livestock and poultry. The traditional Chinese medicine polysaccharide immunoenhancer is prepared from astragalus membranaceus, codonopsis pilosula, poria cocos and lentinula edodes, wherein the weight part ratio of the astragalus membranaceus to the codonopsis pilosula to the poria cocos to the lentinula edodes is 1:1:1:1. A processing method comprises the following steps: grinding the astragalus membranaceus, the codonopsis pilosula, the poria cocos and the lentinula edodes into powder, mixing uniformly according to the weight part ratio, extracting through ultrasonic cell discruption, performing ethanol precipitation treatment through an ethanol solution, performing washing and drying to obtain brown yellow powder, namely the traditional Chinese medicine polysaccharide immunoenhancer, and finally sub-packaging and warehousing the brown yellow powder. The traditional Chinese medicine polysaccharide immunoenhancer can remarkably improve humoral immune response of the livestock and poultry, has an immunoenhancement effect, remarkably increases the survival rate of the livestock and poultry, further can remarkably reduce a feed-meat ratio, can resist influence of viruses on growth and development of the livestock and poultry, has a repair effect on internal organs, injured by the viruses, of the livestock and poultry, and can promote transformation of lymphocytes in livestock and poultry bodies and enhance the bioactivity of nonspecific cellular immune.

The invention discloses a supercritical technology based system and method for recycling nitrogen and phosphor from city sludge. The system comprises a pretreatment system, a supercritical oxidation system, a nitrogen recycling system, a phosphor recycling system, and a biological processing system. A supercritical oxidation technology is used to maximally recycle nitrogen and phosphor from city sludge; nitrogen and phosphor exist in a liquid phase in forms of ammonia nitrogen and phosphate; ammonia nitrogen in the liquid phase is recovered by an ammonia recycling unit; phosphate in wastewateris recovered by a phosphor recovering system, phosphate is precipitated, and phosphor is recycled. The pH of effluent of the supercritical oxidation system is matched with the ammonia recycling unitand the phosphor recovering system; the heat generated by the supercritical oxidation system is matched with the ammonia recycling unit and the biological processing system; the total using amount ofalkalis and required heat are reduced; wastewater can reach the discharge standards, a wastewater harmless treatment is realized, nitrogen and phosphor are maximally recovered, and good environmentaland economic benefits are generated.

The invention provides a method for conducting composite surface modification on pure titanium. The method includes the following steps that primary surface treatment is conducted on the pure titanium; the pure titanium is perforated, and nanoscale silicon carbide particles are laid; stir-friction longitudinal feeding machining is conducted, and a stir layer is formed; secondary surface treatment is conducted on the stir layer; and micro-arc oxidation treatment is conducted. The method has the advantages that by adding the nanoscale silicon carbide particles, the mechanical property of materials is improved; and micro-arc oxidation treatment continues to be conducted on the stir layer, so that a porous composite oxidation layer with biological activity is obtained.

The invention provides a small molecular polypeptide for reforming RGD tripeptide. According to the characteristic that the RGD is specifically combined with alpha<IIb>beta3, a hydrophobic amino acid and a basic amino acid are added at the N end of the RGD, a hydrophobic amino acid W is added in the fourth site, a basic amino acid R is added in the fifth site, the small peptide sequence is Arg-Gly-Asp-Trp-Arg, the protein primary structure is RGDWR, the capability for specifically being combined with the alpha<IIb>beta3 can be improved, the immunogenicity can also be reduced by smaller molecular weight, and the defects of the existing antithrombotic preparations are overcome.

The invention discloses a cat granulocyte colony stimulating factor mutant recombinant fusion protein as well as a preparation method and application thereof, and relates to the field of biological genetic engineering. The recombinant fusion protein is formed by connecting a cat granulocyte colony stimulating factor mutant and a ricin B chain truncated peptide through a flexible linker. The preparation method comprises the following steps: constructing a cat granulocyte colony stimulating factor mutation expression vector; constructing an escherichia coli recombinant expression vector; carrying out the expression of the recombinant FeG-CSF-Mut/RTBD1 fusion protein; and carrying out purification and renaturation on the recombinant FeG-CSF-Mut/RTBD1 fusion protein. The long-acting granulocyte colony-stimulating factor is obtained by connecting the cat G-CSF mutant and the ricin B chain truncated peptide, so that the biological activity of the granulocyte colony-stimulating factor can be enhanced, the protein stability of the granulocyte colony-stimulating factor in in-vivo blood is improved, and the half-life period of a drug in an organism is further prolonged. The recombinant fusion protein has the function of treating leucopenia.

The invention belongs to the technical field of biological gene engineering, and particularly relates to a long-acting canine interferon alpha fusion protein as well as a preparation method and an application thereof. The preparation method comprises the following steps: carrying out codon optimization on canine interferon alpha and CSA truncated peptide, synthesizing the canine interferon alpha and CSA truncated peptide on a plasmid vector, carrying out double enzyme digestion, cloning to obtain a target fragment, carrying out Linker connection to obtain a recombinant plasmid vector protein,carrying out enzyme digestion linearization on recombinant plasmids, and introducing the recombinant plasmids into expression host bacteria to obtain recombinant yeast; and carrying out induced expression, deglycosylation and chromatographic purification to obtain a target protein, namely the canine interferon-alpha fusion protein. The fusion protein is established by adopting an efficient pichiapastoris methanol induced secretory expression system, and meanwhile, CSA truncated peptide and Linker with high stability and long-acting canine interferon alpha with high activity are selected, so that the fusion protein can be used for preparing antiviral drugs.

The invention discloses a microbial improver for improving a soil aggregate structure, and application thereof in soil improvement treatment. Functional microbial agents are Bacillus amyloliquefaciensand Bacillus velezensis, have the effect of promoting crop growth, and can prevent diseases and promote growth while improving soil. Activated humic acid, decomposed tobacco stem and tobacco powder,tobacco stem and tobacco powder activated carbon, clay minerals and the functional microbial agents are used as raw materials to prepare the soil improver for soil improvement and treatment, the pH value of the soil is always kept stable; the permeability of the soil can be improved, the water retention and storage capacity of the soil is enhanced, the cation exchange capacity of the soil is increased, finally, the purposes of improving the soil structure, improving the physicochemical properties, improving the soil fertility, keeping water and soil and improving the contaminated soil are achieved, and good environmental conditions are provided for growth and development of crops and microorganisms and high and stable yield of the crops.

The present invention provides a coenzyme Q10 clear preparation which is added into skin caring articles and a preparation method thereof, and belongs to the technical field of personal caring article preparation. The coenzyme Q10 clear preparation comprises the following components: coenzyme Q10, emulsifier mixture, pH regulator, stabilizer and water. The particle size of microemulsion of the coenzyme Q10 is less than 150nm. The microemulsion solution is yellow clear water solution. Furthermore the coenzyme Q10 clear preparation has the following advantages: low viscosity, better fluidity, and high stability. The coenzyme Q10 clear microemulsion preparation prepared according to the invention overcomes the following defects in coenzyme Q10: low water solubility, low oil solubility, and high difficulty of being added into personal caring article. Simultaneously the preparation performs antioxidation and anti-aging biological activity on the skin. The process for preparing the personalnursing product which comprises the coenzyme Q10 is simplified. A novel dosage form is provided for the application of the coenzyme Q10 on the aspect of personal nursing product.

The invention discloses an antibody or an antigen-binding fragment thereof capable of specifically binding to human LAG-3. The antibody has a brand new epitope binding to the human LAG-3 and good biological activity of enhancing mixed lymphocyte reaction, can be applied to preparation of drugs for cancer immunotherapy, and has good clinical application prospects.

The invention provides a method for improving the biological activity of an anti-CD43 monoclonal antibody. The method comprises the step of adding Kifunensine into the culture process when the monoclonal antibody produces cells. The method provided by the invention can be used for effectively improving the biological activity of the anti-CD43 monoclonal antibody. The operation is simple and convenient; the cost is low; wide industrial application prospects are realized.

The invention discloses a method for preparing a CIK cell by using heparin anticoagulant plasma. The method uses heparin anticoagulant plasma of a cancerous person to induce and amplify lymphocytes in vitro. The method provided by the invention can expanse the CIK cell greatly. The experiment result shows that the number of CIK cells increases significantly from the seventh day to 21th day, and can be up to 40+/-15.0 times of the start number of cells. The number of immune phenotype CD3+CD56+ cells in the CIK cells accounts for high proportion in the total number of cell, and can be up to 10-60% when the cells are cultured to the seventh days to 21th days. The biological activity of the CIK cells is improved. The experiment result shows that the cell killing activity is more than or equal to 70% when the cells are cultured to the seventh days to 21th days.

The invention relates to the technical field of medicine. No siRNA (small interfering ribose nucleic acid) percutaneous administration mode exists in the prior art for lack of a percutaneous administration transfer system. The invention aims at providing a percutaneous administration transfer system of a siRNAmicro emulsion carrier capable of encapsulating the siRNA of relevant genes of specific diseases into the siRNA micro emulsion carrier. The siRNAmicro emulsion carrier protects the stability of the siRNA encapsulated inside the siRNAmicro emulsion carrier, and in addition, the percutaneous capability is high. The invention has another goal of providing a preparation method of the siRNA micro emulsion carrier. The invention provides the siRNA micro emulsion carrier with the proportion of oil phase: emulsifying agents: co-emulsifying agents: water phase being (40-70 percent):(10-20 percent):(10 to 20 percent):(5 to 20 percent). The siRNA micro emulsion carrier has the advantages that macromolecular siRNA can be encapsulated into the water phase, the siRNA is brought into the skin and the mucosa through the skin and the mucosa, the siRNA percutaneous transfer problem is solved, meanwhile, the stability of the siRNA is greatly improved, and the siRNA micro emulsion carrier and the preparation method can be used for siRNA medicine development and scientific research application.

The invention discloses a preparing method of an implant nanotube array with an implant material surface subject to secondary anode and broaching treatment and surface hydrophilicity. According to the technical scheme, the method comprises the steps that firstly, pure titanium or a titanium alloy is provided for machining and molding a dental implant base body, and the base body is subject to mechanical polishing, cleaning, drying, chemical polishing and drying; secondly, the implant base body is placed in an anode of an electrolytic tank to be subject to primary anode oxidization; thirdly, the implant base body subject to primary anode oxidization is placed in a solution to be corroded and oxidized; fourthly, after cleaning, secondary anode oxidization is carried out; fifthly, the base body is placed in a phosphoric acid solution to be subject to broaching; sixthly, after deionized water washing and drying, a contact angle test is carried out, and hydrophilic performance data are obtained; the method has the beneficial effects that pore sizes of the implant nanotube array obtained after secondary anode and broaching treatment are more even in distribution, collagenous fiber size and arrangement in bone tissue can be simulated, the elasticity modulus similar to the bone tissue is obtained, and the implant surface performance is optimized.

The invention discloses a human epidermal growth factor hEGF gene optimization sequence, a method for preparing the same and application of the human epidermal growth factor hEGF gene optimization sequence. The human epidermal growth factor hEGF gene optimization sequence is shown as SEQIDNO.1. The human epidermal growth factor hEGF gene optimization sequence, the method and the application have the advantages that cDNA [complementary DNA (deoxyribonucleic acid)] structures of EGF (epidermal growth factor) genes are changed, alkali bases of individual genes on nucleotide sequences are replaced by synonym codons, genes with the optimal codon usage bias are selected to synthesize EGF optimization sequences, prokaryotic expression vectors pET-30a-hEGF are constructed and can be expressed in Escherichia coli hosts in a high-level manner, and accordingly the human epidermal growth factor hEGF gene optimization sequence, the method and the application have important significance on EGF industrial production and application.

The invention discloses a pesticide composition for regulating plant growth and application thereof. The pesticide composition comprises an active component and a synergistic safener, the active component comprises the following components in parts by weight: 0.001-1 part of brassinolide, 0.3-20 parts of gibberellin and 1-10 parts of diethyl aminoethyl hexanoate, and the synergistic safener comprises the following components in parts by weight: 1-3 parts of calcium glycerophosphate and 1-5 parts of cortex phellodendri extract. The pesticide composition is harmless to human bodies and environment, can be used as a fruit tree dormancy breaking agent, has strong permeability and good adhesion to fruit trees, can shorten the dormancy period of the fruit trees and accelerate the dormancy breaking speed of the fruit trees, and is superior to commercially available products. All the components are mixed for use, the functions of all the components are complemented and coordinated, the germination rate of the fruit trees can be increased, the biological activity of the fruit trees is improved, the dormant period of the fruit trees is shortened, and the yield of the fruit trees is increased.

The invention relates to a tea tree dormancy breaking agent as well as a preparation method and an application of the tree dormancy breaking agent and belongs to the field of tea leaf cultivation. Aiming to solve the technical problems, the invention provides the tea tree dormancy breaking agent which is safe and has a good dormancy breaking effect, and the application of the tree dormancy breaking agent. According to the tea tree dormancy breaking agent, an active component is prepared by following steps: a, adding glycerol into a solution containing NO<3-> and Ca<2+> and chelating at 80-100 DEG C for 0.5-4 hours; and b, cooling and adding urea to obtain the active component, wherein the mol ratio of the NO<3-> to the Ca<2+> to the glycerol is (20-55) to (6-10) to 13 and the mol ratio of the urea to the glycerol is (15-28) to 13. The invention further discloses the application of the tea tree dormancy breaking agent in the aspect of promoting the breaking of dormancy of tea trees. According to the tea tree dormancy breaking agent, all the components are harmless to a human body and the environment; complicated chemical components are not added and the residue of hormones is not caused; a safety problem is not caused; meanwhile, the tea tree dormancy breaking agent has the advantages of good dormancy breaking effect and the like.

The invention belongs to the technical field of microalgae polysaccharide preparation, and particularly relates to a preparation method of nannochloropsis oculata polysaccharide with antioxidant activity. An ultrasonic-microwave-enzyme method is used for synergistically extracting the polysaccharide in the nannochloropsis oculata, and during extraction, the cavitation crushing effect of ultrasonic waves on nannochloropsis oculata powder, the high-energy effect of microwaves on the nannochloropsis oculata powder and the efficient and specific property of enzymes on cell walls of the nannochloropsis oculata powder are fully combined, so the dissolution of the polysaccharide in the nannochloropsis oculata powder is increased, and the extraction rate of the nannochloropsis oculata polysaccharide is greatly improved; the extracted nannochloropsis oculata polysaccharide is subjected to alpha-amylase modification to change the structure of the polysaccharide, so the groups and the structure of the polysaccharide subjected to shear modification on the space structure of the polysaccharide are changed, the physicochemical properties of the polysaccharide can be changed to different degrees, and the biological activity of the polysaccharide is improved; the free radical scavenging rate (-Sa/%) of the modified nannochloropsis oculata polysaccharide DPPH can be increased to 78.9% from 61.5% of the modified nannochloropsis oculata polysaccharide DPPH, and the increasing rate is 28.29%.

The invention discloses an ixeris sonchifolia hance preparation with an anti-gout effect. Through the anti-gout effect of the ixeris sonchifolia hance preparation, the clinical indication of ixeris sonchifolia hance is increased, so that the ixeris sonchifolia hance preparation not only can be used for treating a coronary heart disease, cerebral thrombosis, angina pectoris, anti-tumor and the like, but also can be used for treating a gout disease. An ixeris sonchifolia hance extract is taken orally and by injection, has an obvious effect on a hyperuricemia model induced by sodium urate in miceand is close to an allopurinol positive control group in effect. The ixeris sonchifolia hance preparation disclosed by the invention has the benefits that the ixeris sonchifolia hance preparation isa pure traditional Chinese medicine preparation, is small in toxic and/or side effects, has a cardiovascular and cerebrovascular protection effect and is an ideal anti-gout drug.

The invention discloses a double-gene modified stem cell as well as a preparation method and application thereof, and further provides a pharmaceutical composition containing the stem cell. The stem cell comprises a first exogenous nucleic acid and a second exogenous nucleic acid which are located in the same expression vector. The first exogenous nucleic acid encodes a nucleotide sequence comprising a first protein, and the first protein is selected from adiponectin, an adiponectin variant or a first fusion protein comprising the adiponectin or the adiponectin variant. The second exogenous nucleic acid encodes a nucleotide sequence comprising a second protein, and the second protein is selected from sphingosine kinase I, a sphingosine kinase I variant, or a second fusion protein comprising the sphingosine kinase I or the sphingosine kinase I variant. The stem cell modified by double genes provided by the invention shows a synergistic effect of an Adiponectin gene and a mutated SPHKI gene, and can obviously reduce the blood sugar and blood fat of a tested animal and reduce the weight.

The invention discloses a canine interferon mutant recombinant fusion protein as well as a preparation method and application thereof and belongs to the field of biological gene engineering. The canine interferon mutant recombinant fusion protein is formed by connecting a canine interferon alpha mutant and a ricin B chain protein through a flexible linker, wherein the flexible linker is (Gly4Ser)3 connecting peptide. The preparation method of the canine interferon mutant recombinant fusion protein comprises the following steps that: step 1, a CaIFN alpha mutant expression vector is constructed; 2, an escherichia coli recombinant expression vector is constructed; 3, the CaIFN alpha and Mut/RTB fusion protein expression are recombined; and step 4, purification and renaturation are performed. The recombinant fusion protein can be applied to the preparation of antiviral drugs. Compared with common canine interferon alpha, the recombinant fusion protein of the invention has the advantagesthat longer half-life period and higher biological activity. The half-life period of the recombinant fusion protein is prolonged; the biological activity of the recombinant fusion protein is improved;the cost of an interferon preparation is reduced; and the recombinant fusion protein has a potential and value of being developed into broad-spectrum antiviral drugs and in veterinary clinical application.