The invention discloses a method for preparing a soluble HIV-1 integrase recombinant protein, and belongs to the technical field of biology. The preparation method comprises the steps of induced expression, bacteria disruption, purification and the like. The adopted expression medium contains 15 to 35 g/L of tryptone, 10 to 20 g/L of yeast extract, 2 to 3 g/L of sodium chloride, 1 g/L of glucose, 10 to 15 g/L of glycerin, 2.05 g/L of disodium hydrogen phosphate, 1.27 g/L of sodium dihydrogen phosphate, 50 mg/L of magnesium sulfate, and 60 mg/L of kanamycin; and the adopted cracking buffer solution contains 20 mM of 4-hydroxyethyl piperazine ethanesulfonic acid, 1 M of sodium chloride, 2 mM of beta-mercaptoethanol, 0.3 mg/ml of lysozyme and 5 mM of imidazole, and the pH value of the buffer solution is 9.0. The expression medium can ensure normal growth of host bacteria and efficient expression of the objective protein; and the cracking buffer solution can enhance the disruption effect of bacteria, improve the solubility of the objective protein and finally improve the yield of the objective protein.