843results about How to "Improve proliferative ability" patented technology

A novel growing method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.

The present invention belongs to the fields of molecular biology and immunology and relates to a chimeric antigen receptor combining EGFR (epidermal growth factor receptor) family proteins and a composition and uses thereof The present invention particularly relates to the chimeric antigen receptor comprising polypeptides efficiently combining the EGFR family proteins and transmembrane domains, wherein the polypeptides efficiently combining EGFR family proteins comprise HERIN. The chimeric antigen receptor can promote the proliferation ability and killing effect of T cell at the premise of maintaining the killing specificity of the T cell.

The invention relates to the field of biology, and discloses a serum-free culture medium which essentially comprises an IMDM (Iscove Modified Dulbecco Medium), L-glutamine, sodium bicarbonate, Hepes, recombinant human insulin, recombinant human transferrin, recombinant human albumin, 2-mercaptoethanol, protocatechuic acid, lipid, amino acid, vitamins, trace elements, Pluronic F-68, hydrocortisone, vitamin C, bonding amine or recombinant human fibronectin, progesterone, putrescine, heparin, serotonin, epidermal growth factors (EGFs), b-fibroblast growth factors (FGF), platelet derive growth factor (PDGF)-BB and insulin-like growth factor (IGF)-I. The serum-free culture medium is clear in chemical components, free from animal sources and serum and safe and ideal in cell cultivation and avoids the doped animal components and unstable batches, and the results of the cultured mesenchymal stem cells show that the total cellular score, the cell phenotype and the secretory cell factors are normal, so that the serum-free culture medium has good industrial application prospect.

The invention discloses pyridylpyrimidyl amine compounds or pyridylpyridyl amine compounds disclosed as Formula I and application thereof in preparing antineoplastic drugs, belonging to the technical field of drugs. The compounds can selectively inhibit CDK4 and CDK6, enable G1-period tumor cells to stop growth and G1-period tumor cells to reduce, can effectively lower the phosphorylation of the tumor inhibiting protein Rb in the Ser780 site, and thus, can be used for various diseases caused by cell cycle control maladjustment participated by CDK4 and CDK6. The compounds are especially suitable for treating malignant tumors, and have the characteristics of high selectivity, high activity and high tumor cell proliferation resistance.

The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis and treatment of human diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers.

The invention relates to methods of obtaining an expanded population of mammalian ex vivo cells and / or for treating a mammalian subject by (a) administering to a subject an effective amount of an agent that confers a growth disadvantage to at least a subset of endogenous cells at the site of engraftment; (b) administering to the subject an effective amount of a mitogenic stimulus for the ex vivo cells; and (c) administering the ex vivo cells to the subject, wherein the ex vivo cells engraft at the site and proliferate to a greater extent than the subset of endogenous cells, to repopulate at least a portion of the engraftment site with the ex vivo cells. The repopulated cells can be harvested for further use or be left at the engraftment site of a subject to be treated. The invention also provides methods of treating brain injury in a subject by engrafting ex vivo cells at the site of injury.

The present invention relates generally to compositions for wound closure. More specifically, the present invention provides human skin equivalents engineered to express exogenous polypeptides (e.g., antimicrobial polypeptides and keratinocyte growth factor 2) and compositions and methods for making human skin equivalents engineered to express exogenous polypeptides. In addition, the present invention provides methods for treatment of wounds with human skin equivalents engineered to express exogenous polypeptides.

The present invention is directed to cells comprising a recombinant polynucleotide sequence that encodes a telomerase reverse transcriptase protein, variant, or fragment having telomerase catalytic activity when complexed with a telomerase RNA.

The invention relates to the field of stem cells, and discloses a cryopreservation solution and a cryopreservation method for mesenchymal stem cells. The cryopreservation solution for mesenchymal stem cells comprises a serum-free medium, DMSO and dextran-40, wherein the concentration of the DMSO is 3-7 v / v %, and in g / mL, the concentration of the dextran-40 is 1-3 w / v %. After the cryopreservation solution for mesenchymal stem cells disclosed by the invention is used, the survival rate of cryopreserved cells is significantly increased, and good stem cell characteristics are kept. In the cryopreservation method for mesenchymal stem cells disclosed by the invention, after cells are resuspended by using the cryopreservation solution disclosed by the invention and the cell density is adjusted, the cells are cryopreserved. After cells cryopreserved by using the cryopreservation method for mesenchymal stem cells disclosed by the invention recover, the survival rate of the cells is obviously increased, the cell reproductive capacity is also superior to that in conventional cryopreservation methods, and specific surface markers of mesenchymal stem cells also do not have obvious drop, and have a high expression rate.

The invention discloses a preparation method of original mesenchymal stem cells. In the invention, the mesenchymal stem cells are prepared by adopting fresh human umbilical cord, extracting Huatong glue, digesting by collagenase, and separating and cultivating, and the mesenchymal stem cells are suitable for cryopreservation. The invention also relates to the original mesenchymal stem cells prepared by the method, and the original mesenchymal stem cells are verified as mesenchymal stem cells by the identification of biological phenotype according to standard formulated by the International Society of Cytotherapy. The multiplication capability and the purity of the mesenchymal stem cell are both higher than those of bone mesenchymal stem cells; and the amount of the released growth factorsand cell factors is higher than that of the bone mesenchymal stem cells.

An interleukin-15 (IL-15) gene modified natural killing cell strain for immunotherapy of tumor is prepared through inserting the cDNA coding region of IL-15 in pcDNA3 eucaryotic expression carrier, configuring secretion-type recombinant eucaryotic expression carrier pc DNA3 / IL-15, using liposom to transfecte cell NK-92, adding Geneticin (G418) to screening culture medium, screening, limited dilution to cloned cells, detecting activity, choosing positive cell clones, and amplification.

The invention discloses a method and application for inducing human umbilical cord mesenchyme stem cells to be differentiated into testicular interstitial cells. The method comprises the following step of culturing human umbilical cord mesenchyme stem cells of patients suffering from adenovirus and carrying mice steroidogenic factor-1 genes in a DMEM-F12 culture solution containing 0.3-3ng / ml of luteinizing hormone, 200-800mu M of dibutyryl cyclic adenosine monophosphate, 5*10<-6>-5*10<-4>M of all-trans retinoic acid (ATRA), 10mU / ml of human chorionic gonadotropin and 2.4uM of adrenocorticotrophic hormone for a week. Induced by the method in the invention, the human umbilical cord mesenchyme stem cells can be differentiated into testicular interstitial cells in vitro and provides important sources of cells for treating testosterone shortage by the cell replacing method or the genetic method.

The invention relates to a single chimeric converter for a T-cell signal and application thereof, which belongs to the fields of molecular biology and immunology. Specifically speaking, the single chimeric converter is formed by connection of a polypeptide highly efficiently bonding with immunosuppression molecules on the surfaces of tumor cells and / or tumor matrix cells to a transmembrane domain originated from a high-affinity receptor and an intracellular peptide fragment of a costimulatory signal molecule through a hinge structure. Out-membrane polypeptide receives a signal of the immunosuppression molecules on the surfaces of tumor cells and / or tumor matrix cells and transmits the signal into a cell, a second signal of immune cells is activated through the intracellular peptide fragment of the costimulatory signal molecule, so multiplication capacity of the immune cells and the secretion function of cell factors are enhanced, and the survival time of the activated immune cells is prolonged; thus, side-effects of a tumor immunosuppression microenvironment on adoptive cell therapy effector cells are overcome.

The invention discloses a pyrimido or pyridopyridone compound shown in the formula I and its preparation method and use and belongs to the technical field of drug preparation. The pyrimido or pyridopyridone compound can efficiently and selectively inhibit cyclin-depedent kinase (Cdks) CDK4 and CDK6 activity and prevent tumor cell division through inhibiting CDK4 / CDK6. The pyrimido or pyridopyridone compound can be used for treating various diseases caused through imbalance of cell cycle control based on CDK4 and CDK6 and is especially suitable for cancer treatment.

The invention discloses an anti-ageing skincare product. The anti-ageing skincare product comprises 0.001 to 3 weight percent of a plant stem cell active matter and 0.01 to 3 weight percent of Dragosine carnosine, wherein the plant stem cell active matter is at least one of a Phytocelltec<TM> Malus Domestica apple stem cell, a Phytocelltec <TM> Solar Vitis grape stem cell, a Phytocelltec <TM> Alp Rose rose leave stem cell, a Phytocelltec <TM> Argan nut stem cell and a Regenistem Rice Ap red rice stem cell. The preparation method of the anti-ageing skincare product comprises a step of adding the plant stem cell active matter and the Dragosine carnosine into a skincare product. The anti-ageing skincare product comprises the plant stem cell active matter and the Dragosine carnosine at the same time, and under the synergistic action of the two components, the anti-ageing skincare product can obviously improve skin quality, skin elasticity and proliferative capacity of skin, and the effect is obviously higher than that of a skincare product in which the plant stem cell active matter or the Dragosine carnosine is added singly.

The invention relates to a probiotic composition and a preparation. The composition consists of three main probiotics, namely lactobacillus, bifidobacteria and streptococcus thermophilus. The composition can be regulated to be suitable for different intestinal canal characteristics of more people and meet the market requirement and has the advantages of safety, reliability and obvious effect.

The invention relates to a microbial agent and a preparation method as well as an application of the microbial agent. The microbial agent comprises functional bacteria and an adsorbent and is characterized in that the functional bacteria comprise bacillus subtilis, bacillus megatherium, trichoderma longibrachiatum and aspergillus niger, wherein the mass ratio of the microbial fermentations of the bacillus subtilis, the bacillus megatherium, the trichoderma longibrachiatum and the aspergillus niger in a mixture is 0.5-2:0.5-2:0.5-2:0.5-2. The microbial agent is prepared by carrying out the processes of slant culture, shake-flask culture, seeding tank fermentation, fermentation tank fermentation, microbial fermentation mixing, absorbent absorption, and low-temperature drying, and the microbial agent can be applied to the production of straw manure.

The present invention aims at providing one kind of natural plant-originated antisenility skin care factor for cosmetics preparation. Bamboo leaf general flavone has the beautifying effect of resisting free radical, resisting oxidation, resisting radiation, resisting bacteria, bacteriostasis, promoting proliferation of skin cells, preventing skin from oxidation damage, reducing melanin synthesis and delaying skin senility; and it has special scent,no irritation to skin and no allergic reaction. It may be applied in various cosmetics, such as nourishing agent, sun block skin care agent, anti-wrinkle whitening agent, face cleaning cream, shampoo, bathing cream, etc.

The invention discloses a method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application, which belong to the technical field of microbial agents. In the invention, an initial strain is clostridium butyricunm JSIM-MCB20040312 with the preservation number of CGMCC (China General Microbiological Culture Collection) No.1647; the butyric acid bacteria powder is prepared by the steps of activation of butyric acid bacteria seeds, microencapsulated embedding of the butyric acid bacteria seeds, viable counting and preparation of butyric acid bacteria powder, wherein the concentration of the obtained bacteria powder is not less than 1.0*1011 / g. The viable organism propagation capacity of the butyric acid bacteria subjected to microencapsulated embedding is enhanced; a strict anaerobic environment created by liquid sealing of nitrogen gas or carbon diode and other inert gases or paraffin is avoided; a high-concentration bacteroid can be obtained without introducing air into a general fermentation tank for culturing; the production cost is low and the requirement for fed microbial additives on viable count can be met; the butyric acid bacteria powder also has the characteristics of heat resistance, acid resistance and favorable entericsolubility and is suitably used as animal intestine probiotics; and intestinal diseases of livestock and poultry farming animals such as chicken, pigs and the like are effectively prevented and the growth of the bred animals is promoted.

The present invention relates to process and apparatus for preparing semi-solid slurry for casting, and aims at raising quality of semi-solid slurry. The process of preparing semi-solid slurry with chilling and / or mechanical vibrating includes making molten metal liquid flow to the casting mold in a flow guide device with cooling effect, controlling the temperature of the flow guide device below liquid temperature of the metal for the metal liquid to grow crystal via chilling, flushing the fine crystal with the flowing metal liquid to make it produce necking, crack and breaking and fuse into the liquid flow so as to form semi-solid slurry, and injecting the slurry into casting mold to complete semi-solid casting. The present invention has the features of low cost, simple technological process, and capacity of obtain semi-solid slurry with rich well dispersed and well nodularized solid particles.

The invention discloses a serum-free medium of a stem cell, which comprises a basal medium and an additive, wherein the basal medium is DMEM / F12 (Dulbecco Modified Eagle Medium / F12); the additive comprises 2-15%(v / v) of serum replacement, 20-100ug / ml vitamin C, 0.5-10ng / ml stem cell growth factor, 5-20ng / ml human platelet-derived growth factor and 1-5mmol / ml L-glutamine. The serum-free medium of the stem cell for cell culture has the advantages of short cell cycle, strong multiplication capacity, good cell uniformity and high purity, effectively inhibits differentiation of the stem cell and adherence growth of an endothelial cell, ensures purity and dryness of the stem cell and is free from ingredients of animal origin such as fetal calf serum, and the stem cell obtained by the medium is suitable for clinical application.

A compound Cu(DDC)2 with relatively high tumor-inhibiting activity can be used for treating cancers such as melanoma, breast cancer, lung cancer, liver cancer, and the like. The treatment effect is broad, and the compound has certain selective killing effect against tumor cells. The invention relates to an Cu(DDC)2 injection protein nano-particle preparation used for treating tumor, and a preparation method thereof. Therefore, problems of poor water-solubility and difficulty in intravenous administration of Cu(DDC)2 are solved. According to the invention, with an ultrasonic method, a high-pressure homogenization method, and a micro-jet method, the Cu(DDC)2 injection protein nano-particles are prepared. The Cu(DDC)2 protein nano-particles are prepared from the components that (Cu(DDC)2 + organic solvent) :(protein + water) = 1:2-1:30, wherein the specification of Cu(DDC)2 is 0.01-5mg / mL, the specification of protein is 0.05-25% (w / v), and a ratio of organic solvent to water is 1:2-1:30. Proteins such as human serum albumin (HSA), bovine serum albumin (BSA), hydrophobic protein, glycoprotein, and lipoprotein or polypeptide is adopted as a carrier and a stabilizer for coating medicine particles, such that the particles have good biocompatibility and high security. Cu(DDC)2 tumor-inhibiting treatment effect is improved, and adverse reaction is reduced.

The invention discloses a BC (Bacterial Cellulose) / PVA (Polyvinyl Alcohol) composite material, as well as preparation method and application thereof. The preparation method comprises the following steps of: (1) preparing a medical BC wet film block; (2) preparing a polymer solution preparation, namely, under the conditions of high temperature of 95-130 DEG C and high pressure of 0.05-0.15 MPa, preparing a 20-40wt% PVA water solution, namely the polymer solution; (3) soaking the BC wet film block in the polymer solution; and (4) carrying out freeze thawing crosslinking to form the composite material. According to the PVA / BC composite series material prepared by the preparation method, when the PVA concentration reaches 20%, the modulus of compression reaches more than 30 MPa, especially, when the PVA concentration reaches 28%, the modulus of compression reaches more than 50 MPa, the composite material can be used for preparing a replacement for meniscus or cartilage tissue, or preparing replication products of meniscus or cartilage tissue, and the composite material can meet the mechanical strength requirement of bearing tissues, such as the foot cartilage and the meniscus.

The invention discloses a method for in vitro culture of high-proliferation and high-mortality NK cells. The culture method comprises the following steps: (1) performing coating of anti-human CD3 monoclonal antibodies with a concentration of 0.01-3.0 mg / ml and anti-human CD16 monoclonal antibodies with a concentration of 0.01-3.0 mg / ml in a cell culture bottle, causing the coating conditions to be room temperature, causing the incubating time to be 0.5-6 h; (2) causing mononuclear cells obtained through separation out of peripheral blood to be inoculated in the cell culture bottle through serum-free culture solution, and performing stimulation for 12-24 h; (3) adding serum-free culture solution for NK cell culture, IL-2 with a concentration of 20-50 ng / ml, IL-15 with a concentration of 20-50 ng / ml and PedinophyllolB with a concentration of 30-80 ng / ml, performing stimulation for 12-84 h, and then transferring the solution into a cell culture bag to go on performing culture; and (4) obtaining NK cells. According to the method, the high-proliferation and high-mortality NK cells can be obtained, and the method can be used for tumor cell immunotherapy.

The present invention discloses a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells (iPS cells) into hepatocytes, a method for inducing the differentiation of embryonic stem cells or induced pluripotent stem cells into hepatic endoderm cells, and a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells into hepatic progenitor cells. The present invention also provides the hepatocytes, hepatic endoderm cells and hepatic progenitor cells obtained by above methods, and the uses of these cells.

The invention provides a stem cell preparation for treating hepatic fibrosis, and a preparation method of the stem cell preparation. The stem cell preparation for treating hepatic fibrosis is prepared from human menstrual blood mesenchymal stem cells, a chemotactic factor CXCL8 and normal saline. The obtaining method of the menstrual blood mesenchymal stem cells is simple, free of ethical restriction, high in cell viability and high in proliferation capacity, has multi-directional differentiation potential and is simple and convenient to prepare. The menstrual blood mesenchymal stem cell preparation infusion technology provided by the invention is simple and convenient to operate; the hepatic fibrosis is treated by virtue of the menstrual blood mesenchymal stem cells; and the developed preparation method of the stem cell preparation for treating the hepatic fibrosis has very important clinical significance and wide application prospect.

The invention provides a method for preparing a tissue engineering blood vessel based on a 3D (3-Dimensional) bioprinting technology. The method comprises the following steps:alternately printing a blood vessel shape by virtue of a high-polymer material and a human renascent skin fiber cell column, and removing the high-polymer material after fusion to structure a tissue engineering blood vessel; filling late-outgrowth endothelial progenitor cells into the blood vessel, and performing in-vitro dynamic culturing for 7 days in a bioreactor to obtain a differentiated tissue engineering blood vessel, wherein the high-polymer material is one of pluronic F127, poly(N-isopropylacrylamide), methyl cellulose and sodium alginate. The blood vessel obtained by the method is high in biocompatibility and higher in mechanical property, thrombus formation resistant and platelet adhesion resistance, and can be used for repairing and replacing a damaged or disease blood vessel.

The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis and treatment of human diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers.

The invention relates to a Chinese medicinal preparation having functions of enhancing immunity, promoting digestion and improving sleep. The Chinese medicinal preparation is prepared from the following Chinese herb medicines: rhizoma polygonati, medlar, spiny data seed, Chinese yam, tuckahoe, Chinese date, hawthorn, endothelium corneum gigeriae galli, fructus cannabis, rhodiola root, rhizoma atractylodis macrocephalae, epimedium, ganoderma lucidum, tortoise-shell glue and the like. The Chinese medicinal preparation has functions of tonifying kidney and spleen, invigorating Qi and nourishing blood, promoting blood circulation to dispel blood stasis and harmonizing internal organs and is used for treating and relieving senility symptoms, such as tiredness and fatigue, inappetence, insomnia and amnesia, soreness of waist and knees, dizziness or lightheadedness, nycturia, osteoporosis and susceptibility to common colds, which are caused by the deficiency of liver and kidney and hypoimmunity, of middle-aged or old people. Furthermore, the Chinese medicinal preparation can restore the immunity of middle-aged or old people and effectively treat and prevent cardiovascular diseases. The Chinese medicinal preparation has health-care functions of immunoregulation and senility deferment, is safe, does not have side effects, and can be taken for a long time.