8results about How to "Improve proliferative ability" patented technology

The application belongs to the field of treating blood vessel-bone coupling imbalance diseases in traditional Chinese medicine, and particularly relates to a composition for treating blood vessel-bone coupling imbalance diseases, a pharmaceutical preparation and application. Geniposide alone or in combination with crocin I can effectively reduce endothelial cell damage under pathological stimulation conditions, reduce the level of cell apoptosis and the degree of oxidative stress, improve the vascular endothelial function state, and can also significantly inhibit osteoclast differentiation, reduce the number of mature osteoclasts, and reduce bone resorption activity, thereby being conducive to restoring the dynamic balance between bone formation and bone resorption.

The present application provides a method for in vitro proliferation of cells, comprising the following steps: adding a NKG2A conjugate to a first cell culture solution to form a second cell culture solution; and adding a cell to a cell culture container containing the second cell culture solution for a cell expansion culture to obtain an expanded cell. The method for in vitro proliferation of cells provided by the present application can efficiently obtain a large number of high-purity NK cells, and the expanded cells have a significant tumor killing ability.

This invention discloses a network-like porous poly(3,4-hydroxybutyrate) microsphere, its preparation method, and its applications, belonging to the field of biomedical materials technology. The microspheres exhibit a villous network structure with a continuous, irregular "ridge-valley" topology on their surface. The network-like porous poly(3,4-hydroxybutyrate) microspheres provided by this invention maintain good structural integrity even after 6 months of in vivo implantation, significantly increase ATP levels in fibroblasts under glucose-free conditions, and inhibit cell senescence. They also induce macrophages to transform from a pro-inflammatory M1 phenotype to a pro-repair M2 phenotype, avoiding the excessive inflammatory response commonly seen in PLLA-type microspheres, and demonstrate superior collagen regeneration promotion compared to PLLA-type microspheres. Furthermore, they facilitate cell adhesion and proliferation.

The application discloses a method for culturing sweat gland cells to obtain a sweat gland organoid and application thereof, and belongs to the technical field of bioengineering, wherein the sweat gland cells can be cultured by using a three-dimensional culture medium provided by the application, and a sweat gland organoid with high cell activity and bidirectional differentiation potential is obtained; after being transplanted into a full-thickness injury mouse on the sole and back, the sweat gland organoid not only can promote the regeneration and function recovery of the sweat gland, but also can promote the healing of the skin wound, and can be used for preparing a cell product for repairing skin injury and recovering the function of an accessory organ, and has a wide application prospect.

The invention discloses application of a taste sense 2 receptor member 31 (TAS2R31) as a knockout target in cellular immunotherapy, and belongs to the technical field of biological medicines. Specifically, it is found for the first time that the proliferation capacity of tumor infiltrating lymphocytes (TIL cells) obtained after TAS2R31 gene is knocked out is remarkably improved, the killing capacity is remarkably enhanced, and the cell phenotype can be converted towards the direction more beneficial to anti-tumor immunity; meanwhile, the TIL cells with the TAS2R31 gene knocked out can release functional cell factors after being stimulated by antigens, and the TIL cells have definite anti-tumor immunocompetence. Therefore, the TAS2R31 is used as a new knockout target and is used for developing a new tumor treatment scheme based on TIL cells or TCR-T cells. The cell therapy provided by the invention provides a new strategy direction for tumor treatment by improving the multiplication capacity and the immune function of the TIL cells and the TCR-T cells.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

The application belongs to the technical field of microbial fertilizer, and particularly relates to an environment-friendly biological organic fertilizer and a preparation method thereof.The environment-friendly biological organic fertilizer comprises the following raw materials in parts by weight: 70-90 parts of fermented organic matter, 8-13 parts of microbial agent and 10-15 parts of coated suspension.The test results show that after the environment-friendly biological organic fertilizer is applied to soil, the growth of crops can be effectively promoted, the yield of crops is improved, the nutritional quality and edible safety of crop fruits are significantly improved, and the environment-friendly biological organic fertilizer has a wide application prospect.

The invention relates to the field of cell cryopreservation, in particular to a cryopreservation resuscitation fluid for immune cells, the cryopreservation resuscitation fluid comprises NAD < + > or precursor molecules thereof, ethylenediamine tetraacetic acid (EDTA) and human serum albumin (HSA), the invention also provides a method for using the cryopreservation resuscitation fluid, the cell viability can be obviously improved, the cell state can be improved, the cell depletion can be reduced, and the immune cells can be recovered. The good proliferation capacity of the cells is maintained.