19results about How to "Improve proliferative ability" patented technology

The invention relates to a mesenchymal stem cell serum-free culture medium with limited chemical components as well as application and a method thereof, the serum-free culture medium is completely serum-free and has definite components: all additives are known chemical substances and meet the production requirements of cGMP (Complementary Good Manufacturing Practice); telomere shortening can be obviously delayed; replicative senescence is slowed down by activating TERT expression; the multiplication capacity can also be enhanced; the P10 generation cells still have the active division capacity; multidirectional differentiation is supported; the osteogenic differentiation efficiency is superior to that of a traditional serum-containing system; and the method is suitable for industrial production and clinical transformation.

The application discloses a method for culturing three-dimensional cell spheres of alveolar type II epithelial cells under a condition without exogenous matrix glue, and comprises the following steps: A, perfusion digestion treatment is performed on mouse lung tissue to obtain a single cell suspension; B, the single cell suspension is inoculated into a culture dish coated with anti-CD31 antibody and anti-CD45 antibody for immunoadhesion culture, under the culture condition, cells expressing CD31 and CD45 are subjected to immunoadhesion, meanwhile, fibroblasts with strong adhesion ability are attached to the surface of the culture dish, so that non-epithelial cells are removed, and unattached cells are collected to obtain an enriched ATII cell population; and C, the unattached cells are inoculated into a low-adhesion culture environment for suspension culture, and the culture system contains signal regulatory factors for maintaining the stability of the ATII cell phenotype, and the enrichment degree of epithelial cells is improved, and the interference of non-target cells is reduced through the combination of negative immunoadhesion of the anti-CD31 antibody and the anti-CD45 antibody and short-time adhesion treatment.

The invention relates to a CD70 and BCMA targeting dual-targeting chimeric antigen receptor and an application thereof. The CD70 and BCMA targeting dual-targeting chimeric antigen receptor has specific binding ability to CD70 and BCMA. The immune effector cell prepared on the basis of the double-targeting chimeric antigen receptor has the following advantages that the probability that tumor cells escape treatment by losing a single antigen is greatly reduced, and the targeting treatment effect is improved; a CD70 nano antibody sequence is used, so that the length of an insertion fragment is greatly shortened, and the development difficulty of a process end is reduced; and the polypeptide has excellent proliferation capacity, is beneficial to prolonging the in-vivo duration time, and plays a more remarkable anti-tumor role. The invention provides a treatment or improvement approach for diseases related to BCMA-CD70 expression.

The application belongs to the field of treating blood vessel-bone coupling imbalance diseases in traditional Chinese medicine, and particularly relates to a composition for treating blood vessel-bone coupling imbalance diseases, a pharmaceutical preparation and application. Geniposide alone or in combination with crocin I can effectively reduce endothelial cell damage under pathological stimulation conditions, reduce the level of cell apoptosis and the degree of oxidative stress, improve the vascular endothelial function state, and can also significantly inhibit osteoclast differentiation, reduce the number of mature osteoclasts, and reduce bone resorption activity, thereby being conducive to restoring the dynamic balance between bone formation and bone resorption.

The invention discloses a crocodile blood composition for improving immunity and application. The composition provided by the invention comprises the following raw materials in parts by weight: 30-60 parts of crocodile plasma powder, 1-7 parts of liquorice, 5-10 parts of radix asparagi, 3-10 parts of schisandra chinensis, 4-10 parts of codonopsis pilosula, 8-15 parts of lucid ganoderma, 2-8 parts of American ginseng and 1-5 parts of vitamin C. The composition is rich in various active substances capable of improving immunity, and can improve the immunity of the crocodile blood, promote the release level of immunomodulatory factors TNF-alpha and IL-2, increase the total number of spleen and lymphocytes and the volume of white marrow and lymph node cortex of the spleen through the cooperation of specific Chinese herbal medicine components and bioactive components in the crocodile blood, and improve the immunity of the crocodile blood. The immunosuppression effect is reduced, and the lymphocyte proliferation capacity is remarkably improved. The composition provided by the invention is green and safe in raw materials, rich in nutrition, and capable of enhancing the effect of crocodile blood and enhancing the immunity of the organism.

The invention discloses hydrogel containing 7-amino acid phosphorylated peptide and application of the hydrogel in neural restoration. The hydrogel comprises acellular matrix hydrogel, 7-amino acid phosphorylated peptide, 7-amino acid phosphorylated peptide, 7-amino acid phosphorylated peptide and 7-amino acid phosphorylated peptide, and a 7-amino acid phosphorylated peptide supported in the acellular matrix hydrogel. The 2, 7-amino acid phosphorylated peptide can play a multi-dimensional regulation role in nerve injury microenvironment remodeling, realizes dynamic targeted regulation of peripheral nerve injury microenvironment, and promotes rapid regeneration and recovery of injured peripheral nerves.

The invention discloses a biomarker for diagnosing pulmonary fibrosis and application of the biomarker, and belongs to the technical field of biomarkers in clinical medicine. The biological marker for preparing and diagnosing pulmonary fibrosis is a nerve growth factor NGF, the nerve growth factor NGF can remarkably promote differentiation of lung fibroblast MRC5 cells, and along with expression increase of a receptor TrkA of the lung fibroblast MRC5 cells, the migration capacity and the multiplication capacity of the lung fibroblast MRC5 cells are remarkably enhanced. Meanwhile, the NGF also aggravates the formation of the BLM-induced pulmonary fibrosis of the mouse. Therefore, the nerve growth factor NGF as the biomarker has accurate and specific detection and auxiliary diagnosis on pulmonary fibrosis, especially idiopathic pulmonary fibrosis (IPF).

The present application provides a method for in vitro proliferation of cells, comprising the following steps: adding a NKG2A conjugate to a first cell culture solution to form a second cell culture solution; and adding a cell to a cell culture container containing the second cell culture solution for a cell expansion culture to obtain an expanded cell. The method for in vitro proliferation of cells provided by the present application can efficiently obtain a large number of high-purity NK cells, and the expanded cells have a significant tumor killing ability.

The application relates to the fields of biotechnology and targeted therapy, and particularly relates to a recombinant construct, a CAR-T cell and construction and application thereof. The application verifies the role and mechanism of THEMIS in maintaining the long-term killing of tumor cells of CAR-T through experiments. The CAR-T cell overexpressing THEMIS is constructed, compared with a control group, the proliferation capacity and memory cell formation capacity of the THEMIS-7x19 CAR-T cell are enhanced after antigen stimulation, and the cytokines are in a controllable state, the cell apoptosis and exhaustion are reduced, and the long-term tumor killing capacity can be maintained more effectively. The in-vivo experiment also proves that the THEMIS-7x19 CAR-T cell has stronger anti-tumor effect compared with the control group in a lymphoma tumor-bearing mouse model, and the survival time of the mouse is significantly prolonged.

This invention discloses a network-like porous poly(3,4-hydroxybutyrate) microsphere, its preparation method, and its applications, belonging to the field of biomedical materials technology. The microspheres exhibit a villous network structure with a continuous, irregular "ridge-valley" topology on their surface. The network-like porous poly(3,4-hydroxybutyrate) microspheres provided by this invention maintain good structural integrity even after 6 months of in vivo implantation, significantly increase ATP levels in fibroblasts under glucose-free conditions, and inhibit cell senescence. They also induce macrophages to transform from a pro-inflammatory M1 phenotype to a pro-repair M2 phenotype, avoiding the excessive inflammatory response commonly seen in PLLA-type microspheres, and demonstrate superior collagen regeneration promotion compared to PLLA-type microspheres. Furthermore, they facilitate cell adhesion and proliferation.

This invention belongs to the field of biotechnology, specifically relating to a method for constructing a gastric adenocarcinoma model. The method includes the following steps: breeding Anxa10-Cre / + animals or their progeny with LSL-KrasG12D / +;Tp53 R172H / + animals or their progeny to obtain LSL-KrasG12D / +;Tp53 R172H / +;Anxa10-Cre / + animals; administering N-methyl-N-nitrosourea to the LSL-KrasG12D / +;Tp53 R172H / +;Anxa10-Cre / + animals. This method shortens the tumorigenesis period (approximately 2.5 months), increases the tumorigenesis rate (100%), and confirms the pathological type of the obtained gastric adenocarcinoma model as adenocarcinoma.

The application discloses a method for culturing sweat gland cells to obtain a sweat gland organoid and application thereof, and belongs to the technical field of bioengineering, wherein the sweat gland cells can be cultured by using a three-dimensional culture medium provided by the application, and a sweat gland organoid with high cell activity and bidirectional differentiation potential is obtained; after being transplanted into a full-thickness injury mouse on the sole and back, the sweat gland organoid not only can promote the regeneration and function recovery of the sweat gland, but also can promote the healing of the skin wound, and can be used for preparing a cell product for repairing skin injury and recovering the function of an accessory organ, and has a wide application prospect.

The application discloses application of MSCs culture solution in enhancing paracrine and immunosuppression efficiency, wherein a diplotene protein solution is added into a complete MSCs culture medium, i.e., a DMEM / F12 culture medium containing 10% fetal bovine serum, and the MSCs culture solution is obtained after sufficient mixing, wherein the concentration of the diplotene protein in the MSCs culture solution is 1 mu g / ml-500 mu g / ml, and the MSCs culture solution can significantly improve the proliferation capacity, paracrine capacity and immunosuppression efficiency of MSCs without changing the morphology and basic phenotype of the MSCs, the culture solution additive has simple components, can produce significant effects in a short time (24-48h), has wide application prospects, and is favorable for popularization and application.

The invention relates to the technical field of crossing of a stem cell engineering technology, a nano-drug delivery system and a gene therapy technology, in particular to a PEI-LNPmiR-21 delivery system, a preparation method and application thereof and an engineered long-acting stem cell preparation. According to the delivery system, a lipid nanoparticle core is prepared by adopting a microfluidic technology, and low-molecular-weight PEI is covalently grafted on the surface of a lipid layer by utilizing carbodiimide chemistry, so that a nano compound with a core-shell structure is formed. The compound can efficiently deliver miR-21 into stem cells, and by regulating and controlling a PTEN / PI3K / Akt signal channel, the expression of a cell aging marker is remarkably inhibited, the proliferation capacity of the stem cells is enhanced, and the stemness characteristic of the stem cells is maintained.

The invention discloses application of a taste sense 2 receptor member 31 (TAS2R31) as a knockout target in cellular immunotherapy, and belongs to the technical field of biological medicines. Specifically, it is found for the first time that the proliferation capacity of tumor infiltrating lymphocytes (TIL cells) obtained after TAS2R31 gene is knocked out is remarkably improved, the killing capacity is remarkably enhanced, and the cell phenotype can be converted towards the direction more beneficial to anti-tumor immunity; meanwhile, the TIL cells with the TAS2R31 gene knocked out can release functional cell factors after being stimulated by antigens, and the TIL cells have definite anti-tumor immunocompetence. Therefore, the TAS2R31 is used as a new knockout target and is used for developing a new tumor treatment scheme based on TIL cells or TCR-T cells. The cell therapy provided by the invention provides a new strategy direction for tumor treatment by improving the multiplication capacity and the immune function of the TIL cells and the TCR-T cells.

The invention belongs to the technical field of microbial engineering, and discloses bifidobacterium animalis subsp. Lactis JJKK012 capable of producing exopolysaccharides and application of the bifidobacterium animalis subsp. Lactis JJKK012. The problem that the bifidobacterium animalis subsp. Lactis capable of producing exopolysaccharides at high yield and simultaneously exerting antibacterial and immunoregulation effects is lacked at present is solved. The preservation number of the animal bifidobacterium subsp. Lactis JJKK012 is CGMCC (China General Microbiological Culture Collection Center) No.35555, the viable count of the strain is greater than or equal to 1 * 10 < 11 > CFU / g, and the content of exopolysaccharides reaches 856.5 mg / L. The bifidobacterium animalis subsp. Lactis JJKK012 disclosed by the invention can be used for effectively activating RAW264.7 macrophages and exerting a function of enhancing immunity; meanwhile, the strain has good acid resistance, gastrointestinal fluid tolerance, antibacterial ability and cell adhesion ability, and has the function of regulating intestinal flora.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

The application belongs to the technical field of microbial fertilizer, and particularly relates to an environment-friendly biological organic fertilizer and a preparation method thereof.The environment-friendly biological organic fertilizer comprises the following raw materials in parts by weight: 70-90 parts of fermented organic matter, 8-13 parts of microbial agent and 10-15 parts of coated suspension.The test results show that after the environment-friendly biological organic fertilizer is applied to soil, the growth of crops can be effectively promoted, the yield of crops is improved, the nutritional quality and edible safety of crop fruits are significantly improved, and the environment-friendly biological organic fertilizer has a wide application prospect.

The invention relates to the field of cell cryopreservation, in particular to a cryopreservation resuscitation fluid for immune cells, the cryopreservation resuscitation fluid comprises NAD < + > or precursor molecules thereof, ethylenediamine tetraacetic acid (EDTA) and human serum albumin (HSA), the invention also provides a method for using the cryopreservation resuscitation fluid, the cell viability can be obviously improved, the cell state can be improved, the cell depletion can be reduced, and the immune cells can be recovered. The good proliferation capacity of the cells is maintained.