The application relates to a
genetically engineered body, and particularly discloses a
perilla frutescens PfCHLH
gene and a
virus-induced
gene silencing
system, a construction method and application of the
gene silencing
system. PfCHLH The
nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the
amino acid sequence of the encoded
protein is shown as SEQ ID NO. 2. PfCHLH The
gene silencing fragment is cloned into a
virus carrier pTRV2 to obtain a pTRV2- PfCHLH silencing carrier, and the pTRV2 carrier and the pTRV1 carrier are respectively transformed into
agrobacterium GV3101 to prepare infection bacterial liquid; the infection bacterial liquid is mixed according to a volume ratio of 1:1 and then injected into
perilla frutescens cotyledons to induce
gene silencing. PfCHLH qRT-PCR
verification shows that the expression amount is significantly down-regulated and a stable yellowing
phenotype is generated; preferably, the OD 600 of the infection bacterial liquid is 0.6, the silencing
phenotype is more significant, and the consistency is better. The
perilla frutescens VIGS
system established in the application is simple in operation, short in cycle, and high in efficiency, and can be used for
rapid identification of perilla frutescens gene functions and
verification and application of
key genes in related metabolic pathways.