The invention discloses a method for simultaneously detecting 26 organic acids and inorganic negative ions in juice and a juice manufacturing method. According to the method, a juice sample is preprocessed; multilevel-inhibition conductance ion chromatography is utilize to simultaneously detect 18 organic acids (quinic acid, lactic acid, propionic acid, formic acid, butyric acid, pyruvic acid, pentanoic acid, acetic acid, malonic acid, malic acid, citric acid, glutaric acid, maleic acid, fumaric acid, benzoic acid, cis-aconitic acid, trans-aconitic acid, and oxalic acid) and 8 inorganic negative ions (F<->, Cl<->, NO3<->, NO2<->, ClO2<->,BrO3<->, PO4<3->, and CrO4<2->) in a juice; the sample pretreatment is simple, rapid and accurate; the technical problem that multiple components in juice cannot be accurately analyzed is solved, organic acids and inorganic negative ions in juice can be accurately measured, a technical support is provided for judging nutritional value, freshness degree, and true/false of juice, and the components of juice can be detected by the method.