1271results about How to "Achieve enrichment" patented technology

The preparation process of selenium-rich edible fungus with activated selenium ore as selenium source belongs to the field of biological selenium resource converting technology. Natural selenium ore as the selenium source is crushed, activated and water extracted; the selenium containing water solution is used in compounding culture medium; and edible fungus is cultured to realize selenium conversion and enrichment, with the selenium-enriching cultured mycelium containing organic selenium in 50-600 mg/kg. The present invention realizes the conversion and enrichment of organic selenium while producing edible fungus with rich active polysaccharide content.

The invention discloses a method of detecting gene mutation based on Blocker primers and ARMS primers, and a kit. According to the invention, corresponding Blocker primers are designed according to an annealing temperature, the Blocker primers are combined with a wild-type template under the annealing temperature of the Blocker to block amplification of the wild type, and simultaneously an ARMS technology is used to reduce amplification of the wild-type background. The method integrates a Blocker enrichment technology and an ARMS fluorescent quantification PCR technology in one reaction system, and thus realizes enrichment and identification for the mutated genes for one step, reduces operation steps, and improves detection sensitivity and singularity.

The invention relates to a method for carrying out desalination and zero-discharge processing of power plant desulphurization wastewater by adopting an electrodialysis technology. The method comprises the following steps: neutralizing, precipitating, coagulating and filtering the power plant desulphurization wastewater and removing CODs (Chemical Oxygen Demands), heavy metals, F-ions, gypsum, silicon dioxide, hydroxide of iron and aluminum and other insoluble particles in waste water; separating C1-ions and other monovalent ions in clear liquid from SO3<2->, SO4<2-> and other divalent ions through nanofiltration; desalinating and concentrating water produced by the nanofiltration by adopting a multistage countercurrent reverse electrodialysis method. The method has the advantages that the concentration of the Cl-ions in nanofiltration concentrated water and electrodialysis freshwater generated by the method is lower, and the nanofiltration concentrated water and the electrodialysis freshwater can be returned for flue gas desulfurization; the content of NaCl in a small amount of electrodialysis concentrated water produced reaches above 12 percent, the bivalent ions and other impurities are removed by nanofiltration, and the electrodialysis concentrated water is further evaporated and concentrated, so that chlorine-alkali industry electrolytes and NaCl salt can be obtained. Through the method, the zero-discharge treatment and the resource utilization of the desulfurization wastewater can be realized.

The invention discloses a purification process of ganoderma lucidum spores powder. The process comprises the following steps: removing shells of shell-broken ganoderma lucidum spores powder; after immersing by a 20-95% ethanol solution in volume fraction and water, separating shells to obtain a shell-broken ganoderma lucidum spores powder filtrate and shells of ganoderma lucidum spores powder; concentrating and drying the filtrate to obtain purified ganoderma lucidum spores powder; preparing shell alcohol extract solid powder, dichloromethane extract solid powder, ethyl acetate extract solid powder, shell aqueous extract solid powder, normal butanol extract solid powder, water phase powder or chitosan from shells of ganoderma lucidum spores powder by virtue of a comprehensive recycling process. According to the invention, ineffective component (shells) of the spores powder is separated and effective components (polysaccharide, triterpene and the like) are enriched by breaking shells and purifying, so that the content of the effective components of the purified ganoderma lucidum spores powder is remarkably improved and the effect is remarkably enhanced. The shells are comprehensively utilized, so that the economic benefit is improved.

The invention discloses a reagent for extracting and purifying DNA, comprising the following substances: preprocessing lysis solution with pH of 7.4-8.5: water serves as solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg/ml of protease K; pyrolysis combined liquid with pH of 6.4-7.4: water serves as solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10% of surface active agent, 0.5-4% of amphoteric ion detergent, 10-100mM of chelating agent and 15-30% of alcohol; cleaning solution I: water serves as solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of ethanol water solution; spent regenerant is TE (10mM Tris-HCl, 1mMEDTA, and pH is 8.0); magnetic bead suspension is nanoscale silicone coated magnetic microsphere (50-100mg/ml).The DNA extracting efficiency is as high as 93.67% by adopting the reagent.

The invention provides a system and a method for gasification melting of household refuse and treatment of household refuse leachate. The system comprises a household refuse pretreatment system, a refuse energy recovering system and a refuse leachate concentration and flue gas treatment system, wherein the household refuse pretreatment system comprises a household refuse stock bin, a household refuse crusher, a household refuse sieving machine and a household refuse extrusion dehydrator which are sequentially connected; the refuse energy recovering system comprises a fluidized-bed gasifier, a cyclone melting furnace, a waste heat boiler and an air-supplying and oxygen-enriching system matched with the fluidized-bed gasifier, the cyclone melting furnace and the waste heat boiler; the refuse leachate concentration and flue gas treatment system comprises a leachate concentrating system, a flue gas purifying device and a wastewater treatment device. According to the system, the heat value of the refuge is increased by a pretreatment process; an oxygen-enriching gasifier and a high-temperature cyclone melting furnace technology are used as a recovering system of refuge energy; the refuge leachate is concentrated by using medium-temperature flue gas exhausted by the waste heat boiler; the concentrated leachate is sprayed into the high-temperature cyclone furnace for harmless treatment.

The invention discloses a method for extracting and purifying spittle DNA, which comprises the following: 1) a step of pre-treatment, which is to contact the spittle with pretreatment lysis solution, remove solid matters adhered onto cells and obtain coarse lysis solution; 2) a step of nucleic acid absorption, which is to mix the coarse lysis solution obtained by the step 1) with lysis binding solution and magnetic bead suspension to form a solution system containing magnetic nano microsphere and DNA composite and is to collect the magnetic nano microsphere and DNA composite from the solutionsystem; and 3) a step of washing, which is to wash the magnetic nano microsphere and DNA composite obtained by the step 2) with washing liquid I and washing liquid II in turn, and dissolve out DNA byusing eluent. When the method provided by the invention is used, the DNA extraction efficiency may reach 90 percent, the extracted DNA can be used in short tandem repeat (STR) composite amplification, DNA sequencing, DNA quantification and other downstream analysis and operation.

The invention provides a method and system for video enrichment. The method comprises the steps that modeling analysis is carried out on each frame of image in an original video image, and the regional positions of a foreground and background are judged; a binary image is built; each object in the foreground is tracked to obtain an event body; the event body and event body information are stored in a foreground image database; the event body stored in the event body database is divided into event pieces according to preset time intervals, and feature information of the event pieces is obtained; cluster classification is carried out on the event pieces; video enrichment display is carried out; background images are sampled. According to the method and system for video enrichment, event bodies are extracted from a monitoring video automatically, classification and searching are carried out automatically for enrichment display, and therefore video enrichment is achieved, storage space and time needed for watching the video are saved, similar event bodies can be quickly searched out, and browsing and storage of monitoring videos are greatly facilitated.

The invention discloses a method for extracting purified DNA from human exfoliative cells. The method comprises the following steps: 1) pretreating: contacting the human exfoliative cells with pretreating lysate to remove the solid matters adhering to the biological cells to obtain coarse lysate; 2) nucleic acid adsorbing: mixing the coarse lysate obtained in the step 1) with lysis integrated liquid and magnetic bead suspension to form a solution system containing a magnetic nano microsphere-DNA compound and collecting the magnetic nano microsphere-DNA compound in the solution system; and 3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step 2) with washing liquid I and washing liquid II respectively and then dissolving out the DNA with eluent. The efficiency of extracting DNA by the method can reach 90% and the extracted DNA can be applied to such downstream analysis operations as STR (short tandem repeat) multiplex amplification, DNA sequencing, DNA quantifying and the like.

The invention belongs to the field of antimony smelting arsenic alkali residue treatment and particularly discloses a wet-method recycling and harmless treatment process for antimony smelting arsenicalkali residue. The process adopts a whole-wet-method treatment process and mainly comprises the eight working procedures of breaking, dissolving for leaching, oxidation for antimony outlet, purification for impurity removing, transition for crystallizing, reduction for arsenic sinking, evaporative crystallization and secondary residue harmless treatment. Recycling and harmless treatment of the arsenic alkali residue, zero emission of waste residue and waste water and up-to-standard discharge of exhaust gas are thoroughly achieved, the long-term plagued arsenic alkali residue environmental pollution problem in the antimony smelting industry in China is thoroughly solved, and the environmental risk caused by stacking the arsenic alkali residue is eliminated; and meanwhile, separation, enrichment and effective recovering of valuable elements are achieved.

The invention belongs to the technical field of chemical wastewater treatment, and in particular relates to a white carbon black production wastewater treatment technology. The technology comprises the steps of pretreating white carbon black production wastewater so that the water quality of the treated wastewater can reach the water quality requirement of a membrane treatment system; carrying out membrane filtration so that enriching sodium sulfate in the wastewater is enriched at one side of a membrane until the content of sodium sulfate is not less than 10% and the produced water at the other side reaches a recycle water index and is recycled to a production system for reuse; carrying out freezing crystallization on the thick water to separate out mirabilite, separating out the crystallization mother liquor so as to obtain the mirabilite and returning the crystallization mother liquor back to a membrane concentration system, and thermally dehydrating the mirabilite so as to obtain anhydrous sodium sulphate which is not less than 98% and used for selling; or evaporating and crystallizing the thick water with the sodium sulfate being not less than 10% through directly utilizing an evaporator so as to separate out the anhydrous sodium sulphate. The water source recycling and chemical raw material recovery are realized through low energy consumption. Through cooperatively using two technologies, the energy consumption for treating each tonnage of wastewater can be greatly reduced, and the water recovery rate of a whole treatment system is not less than 92%; the sodium sulfate content of an anhydrous sodium sulphate product is not less than 98%.

The invention discloses a tobacco stalk biochar soil conditioner capable of reducing the content of heavy metals in tobacco, improving the tobacco quality and improving the soil environment, as well as an improvement method for tobacco field soil. The tobacco stalk biochar soil conditioner is prepared by mixing the following raw materials in parts by weight: 40-60 parts of tobacco stalk biochar and 40-60 g of a passivant. The tobacco stalk biochar soil conditioner is easy to obtain raw materials, simple in preparation method and low in cost, can effectively reduce the content of heavy metals in tobacco, and can improve the quality of tobacco field soil; a large number of waste tobacco stalks generated in tobacco growing areas are adopted as the raw material, the resource recycling of waste materials is realized, the sustainable development of the tobacco industry is facilitated, and the tobacco stalk biochar soil conditioner is suitable for large-scale popularization and application in the field of tobacco growing.

The invention belongs to the technical field of detection and relates to a pretreatment method for biogen amine neurotransmitter and/or metabolites thereof. The method comprises the following steps of: performing derivatization on a sample containing the biogen amine neurotransmitter and/or the metabolites thereof, and carrying out online solid-phase extraction on derivatization products. On the basis, the invention also relates to a detection method for the biogen amine neurotransmitter and/or the metabolites thereof, a detection kit and application thereof. The pretreatment method has the advantages that impurities in the derivatization products can be eliminated, and after being enriched, the derivatization products with proper concentration are transferred into the following detection steps. The detection method has the advantages that the content of the biogen amine neurotransmitter and/or the metabolites thereof in a biological sample can be fast and accurately measured, and the detection sensitivity is high.

The present invention belongs to the technical field of air purification, and particularly relates to a graphene oxide/titanium dioxide-active carbon three-dimensional composite material and a preparation method thereof, wherein a graphene oxide/titanium dioxide composite material is directly and densely adhered onto the surface of an active carbon filtration screen or a carbon fiber net so as to obtain the three-dimensional composite material. According to the present invention, the graphene oxide/titanium dioxide-active carbon three-dimensional composite material can provide excellent adsorption performance and excellent photocatalytic degradation performance on formaldehyde, wherein photocatalytic degradation is performed after formaldehyde is adsorbed, such that the actual removal of the formaldehyde can be achieved, and the secondary pollution caused by the desorption of the formaldehyde can be prevented; and after the adsorption degradation treatment is performed with the composite material of the present invention, the removal rate of the low concentration formaldehyde (concentration of 1.0 mg/m<3>) in the home within 10 h is up to 82.5-95.6%.