The invention discloses a method for preparing the anode of a microorganism fuel battery with a graphene and ferrous disulfide compound, and belongs to the field of environments, materials and energy. The method comprises the following steps: (1) gradually dropping a ferric trichloride and thiourea solution into a graphene oxide dispersion in a reaction kettle one droplet by one droplet, uniformly stirring, sealing the reaction kettle, and performing hydrothermal reaction for 12-24 hours within 140-200 DEG C so as to obtain a hydrogel sample; (2) washing the hydrogel sample for times with deionized water, performing freeze-drying, and crushing so as to obtain a nano powder of the graphene and ferrous disulfide compound; (3) mixing the nano powder with a 5% nafion solution, isopropanol and deionized water, uniformly oscillating, coating carbon cloth with the solution, fixing the carbon cloth with a fixing part, and drying the carbon cloth in air, so as to obtain the anode. The method has the advantages that the synthesis steps are very simple, obtained particles are uniform in morphology, graphene lamellas are overlapped to form a well-developed porous structure, good electrochemical properties and biocompatibility can be achieved, and the anode of the microorganism fuel battery has very good properties.