The invention discloses a low-temperature plasma generator and a method for preparing the activated water. The device includes a power source, an insulation container and a plasma generating mechanismarranged in the insulation container, wherein the plasma generating mechanism includes an electrode array board, a porous electrode column and a blocking dielectric tube, the electrode array board isconnected with a high-voltage output end of the power source, the porous electrode column is connected with a low-voltage input end of the power source and grounded through a ground wire, the electrode array board includes a connection board and an electrode column array group which consists of high-voltage electrode columns and is arranged at the bottom of the connection board, the porous electrode column is internally distributed with through holes matched with the high-voltage electrode columns, the blocking dielectric tube is arranged between the through holes and the high-voltage electrode columns, and at least two air inlet holes for loading working gas are symmetrically arranged on an upper side wall of the insulation container. The activated water can effectively kill bacteria andfungi live, the inactivation effect on the fungi of a specific species is significant, and most of the fungi (>85.2%) in the liquid can be effectively killed within 1 minutes.