69 results about "Anti nutritional" patented technology

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

The invention discloses high-safety and high-activity chickpea fermentation liquor as well as a preparation method and application thereof. The fermentation liquor is prepared by fermenting chickpeas through specific kefir grain symbiotic flora, the phytic acid content in the raw materials is remarkably reduced to be smaller than or equal to 0.4%, the oxalic acid content is remarkably reduced to be smaller than or equal to 0.07%, the phytic acid content and the oxalic acid content are far lower than those of a water extraction method, an enzymolysis method and a single-strain fermentation method, and potential damage of anti-nutritional factors to scalp is effectively eliminated. Meanwhile, rich active ingredients such as chickpea essence A, small molecular protein peptide and kefir grain lysate are generated in the fermentation process. Experiments show that the fermentation liquor can efficiently inhibit 5alpha-reductase (the inhibition rate is larger than or equal to 80%) and remarkably promote regeneration of mouse hair follicles, has excellent oxidation resistance and malassezia activity inhibition, and can be used for preparing hair products for preventing hair loss and maintaining hair follicles.

The invention relates to the technical field of soybean meal processing, and discloses a soybean meal raw material treatment method, soybean meal and animal feed, the method comprises the following steps: (1) mixing a soybean meal raw material with a pretreatment reagent, and respectively carrying out first extraction, second extraction and solid-liquid separation to obtain a first solid phase; the pretreatment reagent comprises organic alcohol, citric acid, fructo-oligosaccharide and water; the temperature of the second extraction is 15-45 DEG C higher than that of the first extraction; (2) mixing the first solid phase with an enzyme preparation, performing enzymolysis, and performing solid-liquid separation on a product after enzymolysis to obtain a second solid phase; the enzyme preparation comprises phytase, cellulase and non-starch polysaccharide enzyme. By adopting the method disclosed by the invention, the content of antinutritional factors in the soybean meal raw material can be fully reduced, the protein content of the product is increased, the efficient utilization and low immunity characteristics of soybean protein are considered, and the enzymolysis soybean meal product with high protein, low antigen, low small peptide, low bad oligosaccharide, strong stability and good palatability can be obtained without excessive enzymolysis.

The invention relates to saccharomyces cerevisiae MBPSc22 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc22 for synthesizing an immune nutrient, namely cantharidin, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc22 is CGMCC No. 35982; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, and more importantly, the fermented soybean meal is rich in cantharidin, so that the soybean meal protein obtains new functional immune nutrition; therefore, the whole nutritional value of the soybean meal is improved, and the method reaches a leading level in the field of developing functional fermented soybean meal by using saccharomyces cerevisiae.

The present invention belongs to the technical field of microorganisms. Provided are Saccharomyces cerevisiae FNFH_Sc18 and the use thereof. In one aspect, provided is an engineered Saccharomyces cerevisiae strain FNFH_Sc18 for synthesizing immunonutrients β-carotene and astaxanthin, which strain has a deposit number of CGMCC No. 24838. In another aspect, provided is the use of the Saccharomyces cerevisiae. The strain uses soybean meal as a solid-state fermentation substrate, the resulting fermented soybean meal product exhibits significantly increased contents of crude proteins and amino acids, and substantially reduced anti-nutritional factors, such as oligosaccharides and urease. More importantly, the fermented soybean meal is rich in β-carotene and astaxanthin, which imparts new functional immunonutritional properties to a soybean meal protein, thereby improving the overall nutritional value of the soybean meal, and attaining a leading level in the field of developing functional fermented soybean meal by using Saccharomyces cerevisiae.

The invention relates to the technical field of feed production, in particular to a method for producing fermented concentrated feed by extrusion drying, which comprises the following steps: gradient pretreatment, crushing a protein raw material until the particle size is less than or equal to 0.5 mm, adding a complex microbial inoculant, pre-fermenting at 35-40 DEG C for 24 hours, crushing an energy raw material until the particle size is less than or equal to 1.0 mm, and performing extrusion drying to obtain the fermented concentrated feed. The method comprises the following steps: S1, mixing with a pre-fermented protein raw material according to a mass ratio of (4: 1)-(6: 1), adjusting the water content to 40-50%, carrying out enzymolysis synergistic fermentation, synchronously adding a complex microbial inoculant and a complex enzyme preparation into a mixture in S1, carrying out dynamic feed supplement fermentation system, monitoring the pH value and reducing sugar content in a fermentation tank in real time through a sensor, automatically adjusting nutrition supplement according to monitoring data, controlling the fermentation efficiency, and carrying out extrusion drying. According to the production method for achieving fermentation of the concentrated feed through extrusion drying, pre-fermentation treatment is independently carried out on protein raw materials, anti-nutritional factors are accurately degraded, and flora activity inhibition caused by carbon source competition after the protein raw materials are mixed with energy raw materials is avoided.

The invention relates to the technical field of microbial fermentation, and discloses bacillus subtilis, application of the bacillus subtilis in fermented soybean meal and a preparation method of the fermented soybean meal. The bacillus subtilis screened by the invention has strong anti-nutritional factor resistance (such as glycinin, beta-conglycinin, raffinose and stachyose), has a good growth effect in the soybean meal raw material, and is used for fermenting the soybean meal raw material; the content of anti-nutritional factors (such as glycinin, beta-conglycinin, raffinose and stachyose) in the soybean meal raw material can be obviously reduced, and meanwhile, the content of lipopeptide substances (such as surfactin and bacitracin D) in the fermented soybean meal product can be obviously improved; the prepared fermented soybean meal product has a good antibacterial effect on three common intestinal pathogens (escherichia coli, salmonella and staphylococcus) in animal bodies.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The invention relates to a soybean clear water feed capable of remarkably promoting growth of stichopus japonicus and a preparation method and application of the soybean clear water feed, and the soybean clear water feed comprises the following components in parts by mass: 20-40 parts of lactobacillus fermented soybean clear water and 100 parts of stichopus japonicus basal feed. The lactobacillus fermented soybean clear water is obtained by adding lactobacillus bulgaricus into the soybean clear water for fermentation; the preparation method comprises the following steps: fully mixing a stichopus japonicus basal feed, crushing, filtering and screening by using a 100-mesh screen, adding lactobacillus fermented soybean clear water, fully and uniformly mixing, granulating and drying. The soybean clear water stichopus japonicus feed is suitable for stichopus japonicus culture modes such as indoor ponds, outdoor ponds and outdoor net cages. By using the bean clear water, the environmental pollution is reduced, and the feed production cost is reduced; meanwhile, the nutrition is rich, macromolecular proteins, phytic acid, trypsin inhibitors and other anti-nutritional factors in the soybean clear water are fully degraded in the lactic acid bacteria fermentation process, the intestinal flora of the stichopus japonicus can be optimized, the feed utilization rate is increased, the weight gain of the stichopus japonicus is promoted, and the antioxidant capacity of the stichopus japonicus is enhanced.

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.

The invention relates to the technical field of feed production, and particularly provides a compound enzyme and application thereof in enzymolysis of palm meal. The compound enzyme is specially designed according to the nutritional characteristics of the palm meal, non-starch polysaccharide components such as protein, cellulose and mannan in the palm meal can be effectively subjected to enzymolysis, the anti-nutrition effect of non-starch polysaccharide is reduced, the content of soluble oligosaccharide, small molecular protein and the like is increased, and the feeding value of the palm meal is improved.

The invention provides a bacillus halotolans strain with high protease activity, and the bacillus halotolans strain is characterized in that the strain is preserved in Guangdong Microbial Culture Collection Center on July 15, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 64868. The strain is named as CQPL9. The strain can efficiently secrete acidic, neutral and alkaline full-spectrum protease, and can efficiently decompose large protein molecules and anti-nutritional ingredients in soybean meal. The method is suitable for a solid-state fermentation process and can be used for improving the quality of soybean meal, increasing the nutritional value and developing functional products.

The invention discloses a Chinese style functional pastry and a preparation method thereof. Firstly, white kidney beans are induced to germinate, endogenous phytase is activated to reduce the content of resistance factors such as phytic acid and lectin, and the activity of an alpha-amylase inhibitor is protected by spraying a soybean trypsin inhibitor. And then mixing the germinated white kidney beans with the aqueous extracts of astragalus membranaceus and poria cocos, inoculating saccharomycetes and lactobacillus plantarum for fermentation to generate flavor substances and active components, and consuming small molecular sugar to maintain the low GI characteristic. Finally, puerarin powder and the like are added into the white kidney bean paste and the Chinese yam paste to prepare dough, the dough wraps the functional stuffing prepared from the chia seed powder, the Chinese wolfberry fruit powder and the red date and longan paste, and the finished product is obtained through forming, steaming and rose dew incense. The method effectively solves the contradiction between alpha-amylase inhibitor loss and anti-nutritional factor degradation in germination, improves the bioavailability, flavor level and intestinal health regulation function of the product, and realizes the synergistic interaction of nutrition enrichment, blood sugar management and multiple nourishing regulation.

The invention discloses a Lactobacillus paracasei G15 and an application thereof in food with an auxiliary blood glucose reducing effect. The lactobacillus paracasei G15 is classified and named as lactobacillus paracasei, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20232497, the preservation unit is China Center for Type Culture Collection, and the preservation date is December 7, 2023. The lactobacillus paracasei G15 is used for generating organic acid and active peptide through metabolism, improving the fermentation characteristic of dough, and meanwhile achieving the synergistic hypoglycemic effect by adjusting intestinal flora and enhancing insulin sensitivity. The lactobacillus paracasei G15 can also cooperate with Gansu yeast to construct a composite fermentation system, food functions are improved through microbial metabolism conversion, antinutritional factors in food raw materials are decomposed, active peptides are released, short-chain fatty acids are generated, the starch digestion rate is further delayed, and rapid release of glucose is reduced.

This invention belongs to the field of resource utilization and feed processing technology, and discloses a method for preparing Caragana korshinskii feed based on mycelial fermentation. This method utilizes a combination of physical crushing, fine grinding, and microbial fermentation to achieve efficient degradation of anti-nutritional factors without the need for high temperature and pressure. The addition of microorganisms directionally degrades lignin, reducing the lignin and tannin content in Caragana korshinskii, improving palatability, and increasing the crude protein content and digestibility of the feed. Corn and wheat bran are selected as auxiliary materials to improve pellet formation. The low-temperature pelleting process avoids the destruction of nutrients such as amino acids, maximizing the preservation of the feed's nutritional value.

The present application relates to the technical field of microorganism, and particularly relates to a Dvorst's bacteria and a method for fermenting corn steep liquor by using the same.The present application provides a Dvorst's bacteria capable of efficiently degrading vomitoxin in corn steep liquor, and corn steep liquor is fermented by using the Dvorst's bacteria and Candida krusei in cooperation, so that the pH of the corn steep liquor can be increased, the content of vomitoxin and anti-nutritional factors in the corn steep liquor can be reduced, and the content of choline in the corn steep liquor can be significantly increased, the quality of the corn steep liquor is obviously improved, the corn steep liquor after the cooperative fermentation can be better applied in the field of animal feed, and has a wide application prospect.

Provided are a Saccharomyces cerevisiae strain MBP_Sc21 and the use thereof, belonging to the technical field of microorganisms. On one hand, provided is the Saccharomyces cerevisiae strain MBP_Sc21 expressing an immunonutrient β-carotene, the accession number of said strain being CGMCC No. 32663; and on the other hand, provided is the use of the Saccharomyces cerevisiae strain. The strain uses soybean meal as a solid fermentation matrix, the obtained fermented soybean meal product has a significantly increased content of crude proteins and greatly decreased contents of antinutritional factors such as oligosaccharides and urease. More importantly, the fermented soybean meal is rich in β-carotene, which endows the soybean meal protein new functional immunonutrition, thereby improving the overall nutritional value of soybean meal.

The group of inventions relates to the field of processing oily raw material, and more particularly to a novel product in the form of a protein-sugar hydrolysate (PSH) obtained from sunflower meal, a method for producing same by enzymatic hydrolysis, and PSH-based food and feed products. The PSH contains 40-70% protein, 20-40% sugar, and at least 0.02% caffeic acid in free form or in bound form as part of chlorogenic acid isomer molecules. In addition, the PSH has a total branched-chain amino acid content of at least 30 g / kg of the final product (PSH), and an arginine content of at least 20 g / kg PSH. The resulting PSH does not contain antinutrients and serves as an excellent substitute for animal protein in food products and in diets for aquatic organisms, livestock and domestic pets. The claimed novel method is safe, environmentally-friendly, waste-free and allows flexibility in terms of the operations and ingredients used in the production process.

The application discloses a special feed for fattening pigs rich in functional amino acids, which comprises the following components in parts by weight: a core functional amino acid composition 3-5 parts, a low-soybean meal multi-protein matrix 60-70 parts, a nutrition synergistic component 20-25 parts, an anti-nutritional factor degradation agent 1-2 parts, and a micro-ecological and plant extract component 1-2 parts. A preparation method comprises the following steps: obtaining the low-soybean meal multi-protein matrix by pretreating raw materials; preparing the core functional amino acid microcapsule; mixing the basic materials and then performing low-temperature section granulation; after cooling, mixing the micro-ecological and plant extract component at low temperature; and finally screening and removing impurities and unqualified particles to obtain the finished product. The application optimizes the amino acid ratio and the preparation process, protects the activity of the amino acids, adapts to different growth stages of the fattening pigs, improves the growth performance and carcass quality, improves the raw material digestion rate, reduces nitrogen emission, and has strong applicability.

The invention relates to the technical field of feeds, and discloses a functional probiotic-based daily ration formula for laying hens and a preparation method of the functional probiotic-based daily ration formula. Wheat middling is used as daily ration of laying hens, so that the function of reducing the breeding cost is achieved. The laying hen feed is prepared from the following raw materials in parts by weight: corn, soybean meal, 15-30 parts of wheat middling, 0.1-0.2 part of compound probiotic dry powder, 5% laying hen premix and the like according to a scientific proportion, and the compound functional probiotic dry powder is prepared by compounding bacillus licheniformis, bacillus subtilis and enterococcus faecalis. According to the laying hen feed disclosed by the invention, bacillus revives and reproduces in intestinal tracts of laying hens and secretes hydrolases such as xylanase and protease in situ, anti-nutritional factors such as non-starch polysaccharides in wheat middling are efficiently degraded, and wrapped nutrient substances are released; and the lactic acid bacteria regulate the intestinal microecological balance through competitive rejection and maintain the intestinal health. The daily ration can significantly improve the utilization rate of wheat middling, improve the feed-egg ratio, effectively improve the eggshell strength and quality while ensuring the egg laying performance, and assist breeding cost reduction and efficiency improvement.

The present application relates to the technical field of feed, in particular to a functional protein feed produced by using enzymatic decoupling fermentation process to treat the meal and a preparation method thereof. The method mixes double-low rapeseed meal, de-phenol cottonseed protein and peanut meal according to a specific ratio, and sieves to obtain coarse phase and fine phase. By adding slow-release type embedded acid protease wet capsules (type B) to the fine phase, adding fast-release type embedded acid protease wet capsules (type A) to the coarse phase, and adopting the process of swelling the fine phase first and then carrying out anaerobic fermentation with the coarse phase, the time sequence and space control release of the protease in the fermentation process are realized. The technology significantly improves the enzymatic hydrolysis efficiency of protein and the degradation effect of anti-nutritional factors, and the final product has high nutritional value and can effectively improve the growth performance of weaned piglets.

A process of reducing the glucosinolate content in oilseed meal is disclosed. The oilseed meal is derived from Camelina sativa. The oilseeds are conditioned to degrade some of the glucosinolates. The conditioned oilseeds are pressed to expel oil from the seeds. The press cake fraction of the oilseed is extracted with hexane, desolventized and toasted to reduce the glucosinolate content. Defatted meals with low glucosinolate content made by the process are also described.

The application provides a goat complete mixed pellet feed containing coffee peel and application thereof, and the feed comprises the following raw materials in parts by weight: coffee peel 15-30 parts, corn 20-35 parts, soybean meal 10-20 parts, bran 5-15 parts, palm meal 1-3 parts, baking soda 1-2 parts, mineral premix 1-3 parts, vitamin premix 0.5-1.5 parts, compound probiotic preparation 0.1-0.5 parts and compound enzyme preparation 0.05-0.2 parts. The coffee peel is treated by an alkaline hydrolysis-solid state fermentation compound removal process, so that the problem of the influence of the anti-nutritional factors of the coffee peel on the feeding effect is effectively solved; through optimization of the formula, the multiple components are synergistically enhanced, and through adoption of a specific process, the problems of difficult granulation and molding of the feed after the addition of the coffee peel and loss of nutrients are solved. The feed is used for goat fattening breeding, can improve the daily weight gain of the fattening goats, reduces the feed-meat ratio, improves the intramuscular fat content of the mutton and improves the flavor of the mutton.

The application provides a taste-improved linalool embedded microcapsule feed additive and a preparation method thereof. The additive is a microcapsule composed of core material linalool and wall material, and the wall material comprises raw material matrix, black soldier fly larvae powder, fly maggot powder, edible gelatin, edible lactose and emulsifier; the fermented raw material matrix is prepared from bean curd residue, peanut cake, wheat bran and cassava residue through co-fermentation of lactic acid bacteria and yeast. The preparation method comprises the following steps: mixing the raw material matrix and wall material components to prepare a wall material aqueous solution, adding linalool emulsion, and then performing composite coagulation, glutamine transaminase cross-linking solidification and spray drying to obtain the product. In the application, the anti-nutritional factors of raw materials are removed through fermentation, the protein digestion and utilization rate is improved, the pungent smell of linalool is shielded with the help of gelatin and lactose, the volatilization and oxidation of linalool are slowed down, the feed palatability is greatly improved, the product stability is good, and the product can be widely applied to livestock and poultry breeding feed.

The invention discloses a compound feed for improving the immunity of geese and a preparation method, belongs to the field of livestock feeds, and solves the technical problems that the existing goose feed is difficult to intercept and remove macromolecular anti-nutritional factors, so that the intestinal tracts of the geese cannot absorb effective nutrient substances, and the immunity is low. The compound feed comprises basic nutrient components such as wheat flour, corn oil, rice bran, distillers' grains, apple peel, vinegar residues and soybean meal, as well as modified chitosan, modified glucan and probiotics. The modified chitosan and the modified dextran form a reticular structure compound with the pore diameter of 10-20 nm in the small intestine of the goose, macromolecular anti-nutritional factors can be intercepted, micromolecular nutrition can be allowed to pass through, and the modified chitosan and the modified dextran are discharged along with the anti-nutritional factors after being degraded in the large intestine; the probiotics are used for assisting digestion. During preparation, the processes of layered mixing, low-temperature granulation and drying and twice sieving are adopted. The goose feed is balanced in nutrition and controllable in cost, anti-nutritional factors can be removed, the product quality is guaranteed through the process, and the goose immunity is improved through multiple ways.

This invention discloses an animal feed based on the synergistic enzymatic hydrolysis of cottonseed meal and rapeseed meal, and its feeding equipment, relating to the field of animal feed technology. It comprises the following raw materials in parts by weight: 25-30 parts cottonseed meal, 20-25 parts rapeseed meal, 35-40 parts corn flour, 3-5 parts wheat bran, 0.18-0.26 parts compound enzyme preparation, 2.8-4.5 parts nutritional supplement, and 0.4-0.7 parts functional additive. This invention utilizes multi-enzyme synergistic hydrolysis to degrade protein into easily absorbed small peptides, improving crude protein digestibility. Combined with the complementary and precise supplementation of amino acids from the two meal products, feed conversion efficiency is significantly optimized. Through detoxification enhancement and the synergistic effect of adsorbents, the residual levels of anti-nutritional factors are significantly reduced compared to the unoptimized group, greatly improving feeding safety.

The invention provides an astragalus mongholicus fermented product based on compound probiotic synergistic fermentation and a preparation method thereof, and relates to the technical field of traditional Chinese medicine fermentation. The fermented product is prepared by taking astragalus membranaceus as a raw material and carrying out synergistic fermentation by adopting compound probiotics consisting of lactobacillus plantarum, lactobacillus acidophilus and saccharomyces cerevisiae, the preparation method comprises four core steps of radix astragali pretreatment, compound probiotic preparation, inoculated fermentation and post-treatment. Through the synergistic effect of the compound probiotics, the dissolution rate of astragaloside, astragalus polysaccharide and other effective components in astragalus membranaceus can be remarkably increased, the dissolution rate of astragaloside is increased by 30% or above compared with that of an unfermented group, the dissolution rate of polysaccharide is increased by 25% or above, anti-nutritional factors in astragalus membranaceus are reduced, and the bioavailability of the effective components and the palatability of a product are improved; the prepared fermented product is high in stability and good in safety, can be widely applied to the fields of functional food, health care products and traditional Chinese medicine preparations, and is particularly suitable for people needing to enhance immunity.

The present application relates to a kind of saccharomyces cerevisiae MBP_Sc22 and its application, belong to microbial technology field.The present application provides a kind of synthetic immunonutrient-cantharides yellow saccharomyces cerevisiae engineering strain MBP_Sc22 in one aspect, and its preservation number is CGMCC No.35982;The present application provides the application of the saccharomyces cerevisiae in another aspect, and the strain is with soybean meal as solid fermentation matrix, and the crude protein content of the product fermented soybean meal is significantly improved, oligosaccharide and urease and other anti-nutritional factors are greatly reduced, more importantly, fermented soybean meal is rich in cantharides yellow, so that soybean meal protein obtains new functional immunonutrition, so as to improve the overall nutritional value of soybean meal, reaches leading level in the field of using saccharomyces cerevisiae to develop functional fermented soybean meal.

The invention belongs to the technical field of biological medicines, and relates to hydrogel beads for protecting oral peptide medicines and a preparation method of the hydrogel beads. The hydrogel bead comprises a therapeutic polypeptide, a Bowman-Birk inhibitor (BBI) and a pH response type gel matrix, wherein the pH response type gel matrix is formed by sodium alginate, chitosan and calcium ions through ionic crosslinking and polyelectrolyte composite action. The therapeutic polypeptide is preferably a glucagon-like peptide-1 (GLP-1) analogue, such as a semeglutide. According to the invention, a traditional anti-nutritional factor BBI is converted into a polypeptide drug'natural protective agent ', and hydrogel beads are co-embedded. According to the system, rapid release of BBI and slow release of therapeutic peptide are realized through intelligent carrier design, so that a microenvironment for protecting low protease is locally and actively established in intestinal tracts, the oral bioavailability of drugs is remarkably improved, and a new normal form is provided for high-valued utilization and oral delivery strategies of soybean processing byproducts.

The invention provides pea-based yoghourt and a preparation method thereof. The preparation method comprises the following steps: S1, removing impurities from peas, cleaning, soaking, pulping, filtering, centrifuging, and taking supernate to obtain pea milk; s2, flavourzyme is added into the pea milk for enzymolysis, and pea milk enzymatic hydrolysate is obtained; s3, blending the pea milk enzymatic hydrolysate, adjusting the pH value, sterilizing and cooling; and S4, inoculating a leavening agent, performing constant-temperature fermentation, and performing after-ripening to obtain the pea-based yoghourt. By accurately controlling the enzymolysis process of the pea milk, on one hand, combined anti-nutritional factors in the pea milk are effectively released, and on the other hand, macromolecular substances are decomposed into a nitrogen source and a carbon source which are easily utilized by lactic acid bacteria, so that an efficient metabolism foundation is laid for subsequent fermentation, the fermentation period is remarkably shortened, and the acidity of the pea-based yoghourt is improved; meanwhile, peculiar smell in the peas can be covered, the quality of the pea-based yoghourt is improved by applying the method, and the application range of the peas in the field of plant-based yoghourt is widened.