135 results about "Anti nutritional" patented technology

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The present invention belongs to the technical field of biomass resource utilization and microbial fertilizers, and specifically relates to a fertilizer based on oil-tea camellia cattail, a preparation method and its application. The fertilizer described in the present invention is prepared by a collaborative fermentation process of Aspergillus niger, Phanerochaete chrysosporium and Bacillus subtilis. The obtained fermentation product is separated into solid and liquid to obtain a solid, the solid being a solid fertilizer, and the liquid being a foliar fertilizer after post-treatment. Both can be used for the cultivation of crops such as tangerines and oil-tea camellias. Based on the microbial collaborative fermentation process, the content of anti-nutritional factors tannin and tea saponin in the obtained solid fertilizer is significantly reduced, which is less than 1.8 g / kg and 0.1% respectively on a dry basis, while maintaining a seed germination index higher than 95%. According to a planting comparative test, the average yield per mu of oil-tea camellias using the solid fertilizer and liquid fertilizer obtained in this application increased by 12%. The average yield per mu of tangerines increased by about 11%, and the sugar, vitamin C, and trace element contents in tangerines were higher than those using traditional fertilizers.

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention relates to the field of animal feed science and processing, and discloses an efficient fattening type compound feed for meat gamegalors and a preparation method thereof.The preparation method includes the steps that firstly, specific compound plant raw materials and screened compound dominant flora are adopted for multi-step solid-state symbiotic fermentation, and a fermentation base material rich in probiotics, small molecule peptides and low anti-nutritional factors is prepared; secondly, core functional components including high-activity probiotics, plant extracts, functional lipids, antioxidants and the like are subjected to targeted sustained-release microcapsule coating through a multi-layer composite material; and finally, mixing the fermentation base material with targeted slow-release microcapsules and other conventional feed components, and preparing the compound feed by adopting a low-temperature granulation process. The nutritional value and the utilization rate of the feed can be remarkably improved, and functional components are effectively protected and delivered in a targeted manner, so that the growth and development of the meat gamecocks are promoted, the immune function and the oxidation resistance of the meat gamecocks are enhanced, the meat quality is improved, and the overall health level is improved.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for preparing rapeseed meal polypeptide through mixed bacteria combined fermentation of rapeseed meal. The mixed strain composition suitable for fermenting the rapeseed dregs is obtained through screening, and the rapeseed dregs are subjected to combined fermentation by adopting the mixed strain composition, so that the content of soluble peptides in a fermentation product can be increased, and the content of anti-nutritional factors such as glucosinolates in the fermentation product can be reduced; the antioxidant performance of the rapeseed meal fermentation peptide can be improved, and the rapeseed meal fermentation peptide feed has better nutritional efficacy and feed quality and is wide in application prospect.

The invention relates to the technical field of food engineering, and discloses pediococcus acidilactici, a microbial agent containing the pediococcus acidilactici, application of the microbial agent, sorghum fermented food and a preparation method of the sorghum fermented food. The preservation number of the pediococcus acidilactici is GDMCC (China General Microbiological Culture Collection Center) No.65676, and the pediococcus acidilactici can be used for preparing the pediococcus acidilactici. The microbial agent contains pediococcus acidilactici and auxiliary bacteria. According to the sorghum fermented food obtained through fermentation treatment of the fungicide, antinutritional factors such as tannin in sorghum can be converted into urolithin, hydrolysis of protein, fat and starch can be promoted, the utilization rate of the sorghum is increased, the quality and flavor of fermented products are improved, the fermentation period is shortened, the shelf life is prolonged, and high economic benefits are achieved.

The invention provides a method for preparing a protease beverage by fermenting cyperus esculentus powder and a product thereof. Comprising the steps of directional pretreatment, fermentation medium preparation, segmented fermentation and optional blending, specifically, directional pretreatment adopts low-temperature extrusion at the temperature of 60-70 DEG C and ultrasonic treatment at the power of 200-300 W for cooperative treatment, and the fiber structure and antinutritional factors of soybean meal are effectively broken; in the fermentation stage, lactobacillus plantarum, saccharomyces cerevisiae and bacillus coagulans composite flora are inoculated, anaerobic fermentation is performed at the temperature of 35-37 DEG C, the pH value of the system is reduced to 4.0-4.5, and then L-cysteine hydrochloride-vitamin B synergist is added for secondary fermentation in a micro-aerobic environment at the temperature of 38-40 DEG C. The fermentation process provided by the invention is simple, the treatment is convenient, the used equipment is conventional equipment in the food industry, and the method is suitable for large-scale industrial production. Meanwhile, in the prepared fermentation liquor, the protease activity is larger than or equal to 320 U / mL, the total number of viable bacteria is larger than or equal to 1.2 * 10 CFU / mL, the optimum pH of the protease is 3.8-4.8, the optimum temperature is 37-39 DEG C, the protease is highly matched with the human gastrointestinal tract environment, and the method has wide industrialization prospects.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention belongs to the technical field of feed preparation, and particularly relates to a method for preparing a feed raw material through synergistic fermentation of rapeseed dregs and pomace. The method systematically solves the technical problems of anti-nutritional factor residue, pomace decay risk, low fiber utilization rate, unbalanced nutrition and the like of rapeseed meal, and has the core advantages of thoroughness and safety of detoxification, no chemical residue, significantly improved fiber degradation rate, and significantly improved process stability by inhibiting infectious microbe pollution through segmented temperature control. The method provides an industrial feasible path for high-value utilization of the rapeseed dregs and the pomace, and has both economic benefits and environmental benefits.

The invention discloses a method for preparing fermented soybean meal through synergistic fermentation of saccharomyces cerevisiae FNFHSc18 and bacillus subtilis FNFHBS08 and application of the fermented soybean meal, combined fermentation of the saccharomyces cerevisiae FNFHSc18 and the bacillus subtilis FNFHBS08 is utilized, the method has the advantages of being short in fermentation period, high in efficiency and low in cost, the prepared fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is increased. The contents of crude protein and soluble protein are obviously improved, anti-nutritional factors such as antigen protein, oligosaccharide and urease are greatly reduced, and the nutritional value of the soybean meal is comprehensively improved; the invention also provides an application of the fermented soybean meal feed in breeding, and the fermented soybean meal is used for replacing 30% of fish meal protein, so that the final weight, the weight gain rate and the specific growth rate of the largemouth bass can be obviously improved, and the growth performance of bred animals is enhanced.

The invention discloses a prediction model for predicting the utilization rate of standardized mineral elements of wheat feed raw materials on white feather broiler chickens and construction and application of the prediction model, conventional nutritional ingredients and antinutritional factor contents are obtained as input variables, measured values are taken as output variables, and the standard phosphorus utilization rate = 67.54-2.60 * neutral detergent fibers is obtained; the standardized copper utilization rate is equal to 13.00 + 2.03 * crude protein-3.89 * crude fiber + 13.85 * crude ash-2.51 * phytic acid, the standardized iron utilization rate is equal to 10.05 + 3.21 * crude protein-12.16 * crude fiber + 16.68 * crude ash, and the standardized zinc utilization rate is equal to 14.12 + 29.51 * crude protein-5.42 * crude fiber. The method solves the problems of large workload and long time consumption in the existing determination of the standardized mineral element utilization rate of the wheat feed raw material, and can rapidly predict the standardized mineral element utilization rate of the wheat feed raw material on broiler chickens.

The invention relates to a method for preparing fish feed by secondary utilization of traditional Chinese medicine wastes, and belongs to the technical field of fish feed. After the traditional Chinese medicine waste is pretreated to remove heavy metal, primary fermentation of bacillus subtilis and aspergillus niger is carried out, the bacillus subtilis can degrade cellulose, the aspergillus niger decomposes antinutritional factors, then fermentation of lactobacillus lactis and candida utilis is carried out, lactic acid bacteria produce acid to inhibit infectious microbes, flavor substances are synthesized, and palatability is improved. Through traditional Chinese medicine residue pretreatment, multi-strain synergistic fermentation and a scientific feed formula, efficient reutilization of traditional Chinese medicine waste is successfully realized, and the fish feed which is rich in nutrition, high in safety and remarkable in economic benefit is produced.

The invention relates to the technical field of microbial fermentation, and discloses a high-protein pig feed prepared by a microbial fermentation technology, which is prepared by the following steps: S1, mixing soybean meal, corn straw and bran according to a mass ratio of 5: 3: 2, crushing to 60-80 meshes, adding a compound microbial agent and water, and adjusting the water content to 45-50% to prepare a fermentation base material; the method comprises the following steps: carrying out first-stage solid-state fermentation for 48-60 hours under the condition of 35-38 DEG C, introducing sterile air in the fermentation process and maintaining the pressure in a tank to be 0.05-0.1 MPa and the stirring speed to be 30-50 rpm, and by arranging a complex microbial inoculant synergistic fermentation system, when the high-protein feed is prepared from soybean meal, corn straws and other plant raw materials, the high-protein feed can be prepared from the soybean meal, the corn straws and other plant raw materials. The specific compatibility proportion of bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae is screened, the synchronous effect of cellulose degradation, organic acid synthesis and mycoprotein enrichment is achieved, and efficient passivation of anti-nutritional factors of different raw materials is guaranteed.

The invention provides modified wheat bran, a preparation method thereof and application of the modified wheat bran in preparation of low-GI products, and belongs to the technical field of food processing. The invention provides a preparation method of modified wheat bran, which comprises the following steps: inoculating lactic acid bacteria to sterilized wheat bran, and carrying out first fermentation to obtain a lactic acid bacteria fermentation product; and inoculating hericium erinaceus to the lactic acid bacteria fermentation product, and carrying out second fermentation to obtain the modified wheat bran. According to the preparation method disclosed by the invention, the wheat bran is modified by adopting lactic acid bacteria-hericium erinaceus echelon fermentation, the content of antinutritional factor phytic acid in the obtained modified wheat bran is remarkably reduced, the content of soluble protein is remarkably improved, the modified fermentation product contains Hericene A, Hericene C and Hericene D, and the eGI value is 39.74-45.83.

The present invention provides fermented distiller's grains, a preparation method therefor, and a use thereof. In the method, encapsulated Rhodotorula glutinis powder and a bacterial liquid of Bacillus velezensis capable of efficiently degrading crude fiber are inoculated into distiller's grains for mixing and fermentation to obtain fermented distiller's grains. A solid-state fermentation process enables the encapsulated Rhodotorula glutinis powder to avoid oxidation of astaxanthin produced by same, thereby effectively improving the stability of the astaxanthin and ensuring that the fermented distiller's grains can be stored at room temperature for at least 60 days. Moreover, the content of anti-nutritional factors in the fermented distiller's grains is greatly reduced, and the nutritional content is significantly improved, thereby greatly improving the utilization rate of fermented distiller's grains. Adding the fermented distiller's grains to the non-breeding feed diet of pigs, cattle, and sheep and replacing 40-50% of the diet can effectively reduce the incidence of disease.

The invention provides a control method and device of cooking equipment, a readable storage medium and the cooking equipment. The control method of the cooking equipment is applied to the cooking equipment, the cooking equipment comprises a body and a liquid drainage assembly, a cooking cavity is formed in the body, the cooking cavity is used for containing food materials and liquid, the liquid drainage assembly comprises an opening communicated with the cooking cavity, and the liquid drainage assembly is used for draining the liquid out of the cooking cavity. Comprising the following steps: controlling the temperature in a cooking cavity to be heated to a first cooking temperature so as to perform enzymolysis on anti-nutritional factors in food materials; and heating the cooking cavity according to the target heating efficiency until the gas pressure value in the cooking cavity is higher than the gas pressure value at the opening so as to discharge the liquid in the cooking cavity through the liquid discharge assembly. Coarse cereal food materials do not need to be soaked for a long time, and the cooking efficiency of the coarse cereal food is improved while the anti-nutritional factor discharging effect is guaranteed.

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

The invention discloses high-safety and high-activity chickpea fermentation liquor as well as a preparation method and application thereof. The fermentation liquor is prepared by fermenting chickpeas through specific kefir grain symbiotic flora, the phytic acid content in the raw materials is remarkably reduced to be smaller than or equal to 0.4%, the oxalic acid content is remarkably reduced to be smaller than or equal to 0.07%, the phytic acid content and the oxalic acid content are far lower than those of a water extraction method, an enzymolysis method and a single-strain fermentation method, and potential damage of anti-nutritional factors to scalp is effectively eliminated. Meanwhile, rich active ingredients such as chickpea essence A, small molecular protein peptide and kefir grain lysate are generated in the fermentation process. Experiments show that the fermentation liquor can efficiently inhibit 5alpha-reductase (the inhibition rate is larger than or equal to 80%) and remarkably promote regeneration of mouse hair follicles, has excellent oxidation resistance and malassezia activity inhibition, and can be used for preparing hair products for preventing hair loss and maintaining hair follicles.

The present invention relates to a plant protein composition, preferably from legumes, more preferably from peas or faba beans, characterised by the presence of dietary fibres and a low content of antinutritional compounds such as galacto-oligosaccharides. Another aspect of the invention relates to a method for producing these novel legume proteins, preferably from peas or faba beans. The invention also relates to the use of the legume proteins, preferably from peas or faba beans, for the manufacture of food products.

The invention relates to the technical field of soybean meal processing, and discloses a soybean meal raw material treatment method, soybean meal and animal feed, the method comprises the following steps: (1) mixing a soybean meal raw material with a pretreatment reagent, and respectively carrying out first extraction, second extraction and solid-liquid separation to obtain a first solid phase; the pretreatment reagent comprises organic alcohol, citric acid, fructo-oligosaccharide and water; the temperature of the second extraction is 15-45 DEG C higher than that of the first extraction; (2) mixing the first solid phase with an enzyme preparation, performing enzymolysis, and performing solid-liquid separation on a product after enzymolysis to obtain a second solid phase; the enzyme preparation comprises phytase, cellulase and non-starch polysaccharide enzyme. By adopting the method disclosed by the invention, the content of antinutritional factors in the soybean meal raw material can be fully reduced, the protein content of the product is increased, the efficient utilization and low immunity characteristics of soybean protein are considered, and the enzymolysis soybean meal product with high protein, low antigen, low small peptide, low bad oligosaccharide, strong stability and good palatability can be obtained without excessive enzymolysis.

The invention relates to saccharomyces cerevisiae MBPSc22 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc22 for synthesizing an immune nutrient, namely cantharidin, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc22 is CGMCC No. 35982; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, and more importantly, the fermented soybean meal is rich in cantharidin, so that the soybean meal protein obtains new functional immune nutrition; therefore, the whole nutritional value of the soybean meal is improved, and the method reaches a leading level in the field of developing functional fermented soybean meal by using saccharomyces cerevisiae.

The present invention belongs to the technical field of microorganisms. Provided are Saccharomyces cerevisiae FNFH_Sc18 and the use thereof. In one aspect, provided is an engineered Saccharomyces cerevisiae strain FNFH_Sc18 for synthesizing immunonutrients β-carotene and astaxanthin, which strain has a deposit number of CGMCC No. 24838. In another aspect, provided is the use of the Saccharomyces cerevisiae. The strain uses soybean meal as a solid-state fermentation substrate, the resulting fermented soybean meal product exhibits significantly increased contents of crude proteins and amino acids, and substantially reduced anti-nutritional factors, such as oligosaccharides and urease. More importantly, the fermented soybean meal is rich in β-carotene and astaxanthin, which imparts new functional immunonutritional properties to a soybean meal protein, thereby improving the overall nutritional value of the soybean meal, and attaining a leading level in the field of developing functional fermented soybean meal by using Saccharomyces cerevisiae.

The present disclosure provides compositions and methods for increasing the utilization of non-digestible fibers (NDFs) in animal feed and reducing the anti-nutritional effects of the NDFs. The method comprises orally administering to the animal a composition comprising lysozyme. More specifically, the method comprises orally administering to the animal a composition comprising a lysozyme, i.e., a C-type lysozyme or a GH-22 lysozyme. The methods and compositions can be used to increase NDF utilization, growth performance, survival rate, or any combination thereof in the non-human animal.

The invention relates to the technical field of feed production, in particular to a method for producing fermented concentrated feed by extrusion drying, which comprises the following steps: gradient pretreatment, crushing a protein raw material until the particle size is less than or equal to 0.5 mm, adding a complex microbial inoculant, pre-fermenting at 35-40 DEG C for 24 hours, crushing an energy raw material until the particle size is less than or equal to 1.0 mm, and performing extrusion drying to obtain the fermented concentrated feed. The method comprises the following steps: S1, mixing with a pre-fermented protein raw material according to a mass ratio of (4: 1)-(6: 1), adjusting the water content to 40-50%, carrying out enzymolysis synergistic fermentation, synchronously adding a complex microbial inoculant and a complex enzyme preparation into a mixture in S1, carrying out dynamic feed supplement fermentation system, monitoring the pH value and reducing sugar content in a fermentation tank in real time through a sensor, automatically adjusting nutrition supplement according to monitoring data, controlling the fermentation efficiency, and carrying out extrusion drying. According to the production method for achieving fermentation of the concentrated feed through extrusion drying, pre-fermentation treatment is independently carried out on protein raw materials, anti-nutritional factors are accurately degraded, and flora activity inhibition caused by carbon source competition after the protein raw materials are mixed with energy raw materials is avoided.

The invention relates to the technical field of microbial fermentation, and discloses bacillus subtilis, application of the bacillus subtilis in fermented soybean meal and a preparation method of the fermented soybean meal. The bacillus subtilis screened by the invention has strong anti-nutritional factor resistance (such as glycinin, beta-conglycinin, raffinose and stachyose), has a good growth effect in the soybean meal raw material, and is used for fermenting the soybean meal raw material; the content of anti-nutritional factors (such as glycinin, beta-conglycinin, raffinose and stachyose) in the soybean meal raw material can be obviously reduced, and meanwhile, the content of lipopeptide substances (such as surfactin and bacitracin D) in the fermented soybean meal product can be obviously improved; the prepared fermented soybean meal product has a good antibacterial effect on three common intestinal pathogens (escherichia coli, salmonella and staphylococcus) in animal bodies.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The invention provides a fermentation inoculant for improving puffing performance and nutritional quality of brown rice and application thereof, and belongs to the technical field of food deep processing. The fermentation inoculant for improving the puffing performance and the nutritional quality of the brown rice is composed of bacillus amyloliquefaciens and aspergillus oryzae according to the ratio of (2-3): 1. Through the synergistic effect of the complex microbial inoculants, cellulase, hemicellulase and protease secreted by aspergillus oryzae can break a brown rice bran barrier and degrade macromolecular protein, amylase secreted by bacillus amyloliquefaciens can improve the starch gelatinization efficiency, and meanwhile the content of antinutritional factors is reduced. The final puffed brown rice powder has the characteristics of high expansion rate, loose texture, high nutrition utilization rate, excellent brewing performance and the like, solves the problems of poor puffing performance, low nutrition absorption and easy caking during brewing in traditional brown rice processing, and is suitable for industrial production of instant nutritious food.

The invention relates to a soybean clear water feed capable of remarkably promoting growth of stichopus japonicus and a preparation method and application of the soybean clear water feed, and the soybean clear water feed comprises the following components in parts by mass: 20-40 parts of lactobacillus fermented soybean clear water and 100 parts of stichopus japonicus basal feed. The lactobacillus fermented soybean clear water is obtained by adding lactobacillus bulgaricus into the soybean clear water for fermentation; the preparation method comprises the following steps: fully mixing a stichopus japonicus basal feed, crushing, filtering and screening by using a 100-mesh screen, adding lactobacillus fermented soybean clear water, fully and uniformly mixing, granulating and drying. The soybean clear water stichopus japonicus feed is suitable for stichopus japonicus culture modes such as indoor ponds, outdoor ponds and outdoor net cages. By using the bean clear water, the environmental pollution is reduced, and the feed production cost is reduced; meanwhile, the nutrition is rich, macromolecular proteins, phytic acid, trypsin inhibitors and other anti-nutritional factors in the soybean clear water are fully degraded in the lactic acid bacteria fermentation process, the intestinal flora of the stichopus japonicus can be optimized, the feed utilization rate is increased, the weight gain of the stichopus japonicus is promoted, and the antioxidant capacity of the stichopus japonicus is enhanced.

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.

The invention relates to the technical field of feed production, and particularly provides a compound enzyme and application thereof in enzymolysis of palm meal. The compound enzyme is specially designed according to the nutritional characteristics of the palm meal, non-starch polysaccharide components such as protein, cellulose and mannan in the palm meal can be effectively subjected to enzymolysis, the anti-nutrition effect of non-starch polysaccharide is reduced, the content of soluble oligosaccharide, small molecular protein and the like is increased, and the feeding value of the palm meal is improved.