32 results about "Keratinase" patented technology

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

This invention discloses an acidic serine keratinase gene and its applications, belonging to the field of genetic engineering technology. This invention screened a novel serine keratinase from the acidic hot spring environment of Niujie, Yunnan. This keratinase naturally possesses characteristics of acid resistance, good thermal stability, and high enzyme activity, allowing it to adapt well to the acidic microenvironment of the skin and temperature fluctuations throughout the product's production, storage, and transportation processes. Furthermore, it eliminates the need for complex stabilization treatments, simplifying the production process, reducing production costs, and extending the product's shelf life. This achieves green and efficient preparation, fundamentally solving key problems of existing keratinases in skin applications, such as irritation, poor stability, and difficulty in storage. It is particularly suitable for the gentle exfoliation of human keratin plugs, demonstrating outstanding application value and broad prospects in the daily chemical skincare field.

A method for decomposition of keratin material, e. g. mixture of feathers, is disclosed. The method comprises treating the keratin material with Bacillus cereus KB043 strain, subsequently with sodium hydroxide and potassium hydroxide, and finally with neutralizing acid. The end product is intended for use as an amino acid fertilizer.

The invention discloses preparation and application of bacillus licheniformis for decomposing pigeon feathers, and relates to the field of environmental resource application in the breeding industry, and the preparation method comprises the following steps: step 1, strain activation: firstly resuscitating a freeze-dried strain, and then culturing and activating by adopting a gradient activation mode; 2, preparing a seed solution, namely culturing the strain activated in the step 1 in a seed culture medium to obtain the seed solution; according to the method, the components of the pre-activation culture medium comprise 1% of corn flour, 0.5% of soybean meal and 0.1% of dipotassium phosphate, the production cost is low, and the preparation feasibility and economic benefits of the bacillus licheniformis are improved; the feather decomposition efficiency is improved by 2-3 times through substrate pretreatment, the residual feather amount is reduced, meanwhile, the fermentation period is shortened to 40-50 hours, and the feather decomposition efficiency of the pigeons is improved; in the fermentation tank, the activity of the keratinase can reach 600U / mL, so that the decomposition efficiency can be improved, subsequent reuse is facilitated, and the utilization rate is increased.

The invention belongs to the technical field of pet food and biological medicine, and discloses a composite probiotic composition with a hair ball biodegradation effect and application thereof. The composition comprises Witzia ciliaris, Bacillus subtilis and a camellia seed extract. According to the present invention, the camellia seed extract with the specific source is adopted as the biological surfactant, such that the oil seal sebum layer on the pet hair surface is emulsified and cracked, the hair surface tension is significantly reduced, the keratinase secreted by the bacillus subtilis is induced to efficiently wet and adsorb on the hair fiber so as to achieve the deep biodegradation of the keratin; meanwhile, the lactic acid generated by the Witzia clostridium is combined to promote intestinal peristalsis, and the decomposition and excretion of hair balls are synergistically realized. Experimental results show that the dry weight loss rate of the composition to cat hair reaches 65-78%, the vomiting frequency can be effectively reduced, and the composition has excellent acid-resistant stability, palatability and safety.

The invention discloses skin mucosa maintenance liquid capable of killing fungi and HPV (human papillomavirus) as well as a preparation method and application of the skin mucosa maintenance liquid, and belongs to the technical field of sterilization and virus removal. The skin mucosa maintenance liquid is prepared from octanol ether, benzalkonium bromide, phenoxyethanol, yermate, parachlorometaxylenol, taetinic acid, chitosan, keratinase, fluconazole, glycerol, borneol, menthol, absolute ethyl alcohol and tween-40. Wherein the skin mucosa maintenance liquid has an effect of regulating apoptosis induction on abnormal cells and an effect on vaginal flora, and has an obvious inhibition effect on cervicitis and colpitis mycotica, namely an anti-inflammatory effect; meanwhile, apoptosis of cells containing HPV16 and HPV18 genes can be induced, shedding of abnormal cells is promoted, diseased cells are killed, and vaginal tissues with pathological changes are removed.

The application provides a method for promoting degradation of waste feather by feather powder. The method is characterized in that feather powder is added to an inorganic salt culture medium containing waste feather as the only carbon and nitrogen source, and then microorganisms with the function of degrading keratin are inoculated to degrade the waste feather. The method has the advantages of short degradation time of waste feather, significantly improved degradation rate and keratinase activity, low cost and easy operation. The application can accelerate the green transformation and high-value application of waste feather, and can screen and enrich germplasm resources for degrading keratin, thereby helping to realize the green and circular high-quality development of the livestock and poultry breeding industry.

The invention relates to the technical field of cosmetics, in particular to a mark fading and repairing composition as well as a preparation method and application thereof. The invention relates to a mark fading and repairing composition which comprises the following components in percentage by weight: 0.1%-5% of dragon's blood extract, 0.01%-0.5% of keratinase and 1%-5% of nicotinamide, the preparation method comprises the following steps: preparing the eutectic solvent; performing ultrasonic-assisted extraction; separation and post-treatment; the invention has the following beneficial effects: the resina draconis extract can inhibit continuous pigmentation caused by acne inflammation residue, promote healing of skin minimal wounds and reduce dermis layer injury of acne mark parts; keratinase specifically hydrolyzes stratum corneum aged keratin, cutin metabolism of acne mark parts is accelerated, and accumulation of pigments in stratum corneum is avoided; nicotinamide inhibits melanocytes from transferring melanin to keratinocytes; through the synergistic effect of the three components, a closed-loop printing fading mechanism of anti-inflammatory repair-keratin metabolism-pigment inhibition is formed, the printing fading effect is more comprehensive and more efficient, and skin repair and barrier protection can be both considered.

The present application relates to a kind of multifunctional pharmaceutical composition of dental comprehensive repair and its preparation method, belong to biopharmaceutical technical field, and pharmaceutical composition includes keratin peptide, coral fungus extract, eggshell derived nano calcium phosphate, catechin zinc complex, hydroxyapatite, modified chitosan-PEG gel and other components.Keratin peptide is the product between 3kDa-10kDa obtained by keratinase enzymolysis to white goose velvet;Coral fungus extract is the product obtained by cellulase, subtilisin and bromelain enzymolysis to coral fungus, and purified by G-25 dextran gel column;Modified chitosan-PEG gel is the product obtained by sodium cyanoborohydride stabilization and glycerol plasticization to the reactant of chitosan and dialdehyde polyethylene glycol.Integrated multiple functions such as antibacterial, mineralization and tissue repair, through optimizing component and dosage form, realize efficient, minimally invasive dental damage repair, and good biocompatibility and long-acting.

The invention belongs to the technical field of microorganism application, and particularly relates to bacillus pacificus YM01 capable of degrading feathers and application of the bacillus pacificus YM01. The bacillus pacificus YM01 is preserved in the China Center for Type Culture Collection on June 23, 2025, and the preservation number of the bacillus pacificus YM01 is CCTCC (China Center for Type Culture Collection) M 20251388. The strain can rapidly grow by taking feather as a unique carbon and nitrogen source and degrade and convert the feather into small molecular compounds such as peptide and amino acid, a culture method of the bacillus pacificus YM01 is optimized, conditions for degrading the feather by fermentation liquor are studied, keratinase BPK1 and chitinase BPC1 are screened out, and the application prospect is wide. The invention further establishes a method for degrading feathers by using the strain and fermentation broth thereof, namely extracellular crude enzyme, and provides a feasible technology for biotransformation and utilization of feather resources.

This invention discloses a BhrPETase keratinase mutant and its application in the degradation of polyethylene terephthalate (PET), belonging to the fields of environmental science and enzyme engineering. The site numbers are based on the amino acid sequence shown in SEQ ID NO:1. The mutant contains substitutions of S14G, H78Y, and S184G relative to SEQ ID NO:1, with the preferred amino acid sequence shown in SEQ ID NO:9. Under conditions of 1 mg enzyme protein / g substrate, 65°C, and 96 h, the preferred mutant exhibits a degradation rate of 90.4% for PET membranes and a half-life of 150 h at 60°C, making it suitable for enzymatic degradation and depolymerization of PET.

The application belongs to the technical field of genetic engineering. The application provides application of a thiol-oxidoreductase in promoting keratin enzymolysis and a method for promoting keratin enzymolysis. The thiol-oxidoreductase is applied to a keratin enzymolysis system, and the enzymolysis effect is obviously improved. Through testing, the enzymolysis effect of the thiol-oxidoreductase combined with a protease on keratin is not only obviously higher than the enzymolysis effect of a single protease, but also is better than the hydrolysis of keratin by a chemical reducing agent with the same concentration. The application retains the advantages of enzyme method "green chemistry" and is friendly to the environment.

The invention relates to the technical field of polypeptide extraction, and discloses a method for extracting crocodile nail peptide with an anti-radiation effect, which comprises the following steps: degreasing and crushing crocodile nails to obtain fine powder, and performing solid-phase kneading on the fine powder and an enzymatic extraction auxiliary composition under an anhydrous condition; adding water, homogenizing, and treating by a high-voltage pulse electric field to obtain a flexible precursor; sequentially adding keratinase and alkaline protease to carry out constant pH relay hydrolysis in a nitrogen-filled micro-positive pressure environment; cooling, adjusting the pH to an isoelectric point, performing coagulation, separating supernate, performing gradient elution through macroporous adsorption resin, and performing vacuum concentration to remove alcohol; finally, the concentrated solution is screened through 3000 Da and 500 Da ultrafiltration membrane bags in sequence, trapped fluid is collected, freeze drying is conducted, and the product is obtained. According to the preparation method disclosed by the invention, the dissolution rate and the purity of the crocodile methyl peptide with the anti-radiation effect are effectively improved by combining solid-phase kneading, a high-voltage electric field with double-enzyme hydrolysis and physical screening, and inactivation and loss of active ingredients are avoided.

The invention relates to a multifunctional pharmaceutical composition for comprehensive tooth repair and a preparation method thereof, and belongs to the technical field of biological pharmacy, the pharmaceutical composition comprises keratin peptide, a coral fungus extract, eggshell derived nano calcium phosphate, a catechin zinc complex, hydroxyapatite, modified chitosan-PEG gel and other components. The keratin peptide is a product between 3kDa and 10kDa obtained by performing enzymolysis on white goose down through keratinase; the coral fungus extract is a product obtained by carrying out enzymolysis on coral fungus through cellulase, subtilisin and bromelain and purifying through a G-25 sephadex column; the modified chitosan-PEG gel is a product obtained by stabilizing a reactant of chitosan and dialdehyde polyethylene glycol through sodium cyanoborohydride and plasticizing through glycerol. Multiple functions of antibiosis, mineralization, tissue repair and the like are integrated, efficient and minimally invasive tooth injury repair is achieved by optimizing components and dosage forms, and biocompatibility and long-term effectiveness are good.

The invention discloses a keratinase gene as well as recombinant bacteria and application thereof. Relates to the technical field of gene engineering. The invention provides a gene, an enzyme, an expression vector, a recombinant bacterium and application thereof. The activity of the keratinase of the obtained strain is screened to obtain the keratin-producing recombinant pichia pastoris engineering strain. The engineering strain constructed by the method is high in keratinase activity and good in stability.

The invention discloses thioredoxin reductase BrgTrxR, the amino acid sequence of the thioredoxin reductase BrgTrxR is shown as SEQ ID NO: 1, the gene is obtained from a Brevibacillus gelatini LD5 strain, the protease is subjected to heterologous expression through a genetic engineering means, the protease is purified through a nickel column and is mixed with keratinase BrgM4 to degrade chicken feather, experimental data shows that the activity of the reductase reaches 15.81 U / mg after the reductase is separated and purified, and the activity of the reductase reaches 15.81 U / mg after the reductase is mixed with keratinase BrgM4. In a mixed degradation system with the temperature of 40-80 DEG C and the pH value of 6.5-7.5, the thioredoxin reductase BrgTrxR can improve the hydrolytic activity of the keratinase BrgM4, after the chicken feather is degraded for 48 h through the synergistic effect of the two enzymes, the concentration of soluble protein reaches 14.45 mg / mL, and the thioredoxin reductase is beneficial to cooperating with the keratinase to improve the keratin degradation efficiency.

The application discloses a kind of strong waterproof high moisture-permeable jacket fabric and preparation method thereof, it is related to fabric technical field.The preparation includes the following steps: step 1: the preparation of TPU film: the spinning solution A and spinning solution B containing different porous chitosan microspheres proportion are formulated;Spinning solution A is spun, and inner layer is obtained;Aldehyde group modified porous chitosan microspheres solution is coated on one side thereof, with the coating surface as receiving layer, spinning solution B is spun on the surface thereof, and outer layer is obtained, dried to obtain composite base film;The composite base film is placed in keratinase solution, pretreated;Then 3-sulfopropyl acrylate potassium salt mixed solution is coated on the surface of outer layer, and ultraviolet light is irradiated at room temperature;Immersed in deionized water, dried to obtain TPU film;Step 2: TPU film is hot-pressed with shuttle fabric to obtain jacket fabric.

The application discloses an eggshell membrane peptide for enhancing bone density and a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of an eggshell membrane peptide for enhancing bone density, which comprises the following steps: vacuum freeze-drying eggshell membranes, crushing the eggshell membranes to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernatant; adding collagenase, performing enzymolysis, centrifuging to take supernatant, and obtaining a primary enzymolysis liquid; adding alkaline phosphatase, performing enzymolysis, centrifuging to take supernatant, and obtaining an enzymolysis liquid; performing ultrafiltration, and drying to obtain the eggshell membrane peptide. The application utilizes keratinase to break the cross-linking of keratin by enzymolysis, utilizes collagenase to release active peptides capable of stimulating the proliferation of osteoblasts by enzymolysis, further utilizes alkaline phosphatase to activate phosphopeptide calcium binding sites by enzymolysis, promotes the deposition of calcium salt, and provides the eggshell membrane peptide for enhancing bone density.

The invention discloses an animal feather keratin extraction method which comprises the following steps: (1) mixing pretreated animal feathers with a tea saponin solution, and stirring to obtain degreased feathers; mixing the degreased feathers with an ethanol solution, and performing ultrasonic treatment to obtain loose feathers; (2) preparing liquid drops from the water phase and the oil phase through a micro-fluidic chip process, standing and curing the collected liquid drops, demulsifying and cleaning to obtain sodium alginate-CaCl2 microspheres; (3) mixing the loose feathers, water, the sodium alginate-CaCl2 microspheres and keratinase, then adjusting the pH value, and carrying out enzymolysis; finally, taking supernate; and (4) adjusting the pH value of the supernate, precipitating, dialyzing the precipitated mixed solution, and freeze-drying to obtain the animal feather keratin. According to the method disclosed by the invention, the slow release of calcium ions is realized by adding the sodium alginate-CaCl2 microspheres, so that the enzyme utilization rate is improved.

This invention relates to the field of waste resource utilization and agricultural fertilizer technology. Specifically, it relates to a method and application for treating bile acid extraction waste. This method co-processes bile acid extraction waste with the products of harmless treatment of diseased and dead livestock and poultry into organic fertilizer, which can be used in tobacco cultivation. This invention proposes an integrated co-processing strategy of "two-stage enzymatic pretreatment - microbial fermentation - fertilization": a composite enzyme preparation is constructed by combining bromelain, acidic protease, keratinase, lipase, and xylanase; a two-stage process of acidic enzymatic hydrolysis and neutral main enzymatic hydrolysis is used to break down the complex organic structure of the two types of waste; ultrasonic assistance can be selected to enhance the degradation effect; then, a two-step high-temperature resistant composite microbial fermentation is carried out to achieve efficient degradation and transformation of organic matter; finally, functional components are added to optimize fertilizer efficiency. This invention achieves the co-resource utilization of two types of waste, and the prepared tobacco organic fertilizer has excellent growth-promoting and disease-resistant properties, combining environmental and economic benefits.

The invention discloses a feed additive based on nano silicon biomimetic mineralization and keratin synergistic enhancement and application, and relates to the technical field of poultry feed. The feed additive based on nano silicon biomimetic mineralization and keratin synergistic enhancement comprises nano silicon, L-zinc methionine, cysteine coated manganese, keratinase and diatomite, and is prepared through the steps of diatomite pretreatment, nano silicon vacuum impregnation, L-zinc methionine coating with a fluidized bed and keratinase fixation. The feed capable of improving the beak keratinization strength of the meat ducks is obtained by adding the feed into a conventional feed and granulating, and is applied to breeding of the meat ducks. According to the feed additive provided by the invention, the bioavailability of the functional components can be improved through the synergistic effect of the core components. Compared with the conventional feed, the feed provided by the invention can improve the duck beak hardness, cuticle thickness and weight gain of the meat ducks, reduce the feed conversion ratio and feather picking incidence rate, reduce the duck beak disability rate of the meat ducks and improve the tibia density of the meat ducks, and has wide application prospects.

The invention discloses a cutinase mutant with remarkably enhanced catalytic efficiency on polyvinyl acetate and application of the cutinase mutant, and belongs to the technical field of enzyme engineering and genetic engineering. The combined mutant C11 provided by the invention shows that the catalytic efficiency on polyvinyl acetate is obviously improved, and the yield of a degradation product acetic acid is 1.73 times that of M8. In addition, the half-life period of C11 at 70 DEG C is 205.9 hours, and the half-life period is prolonged by 5.17 times compared with the half-life period of M8. Meanwhile, the stability of the C11 in an alkaline environment is enhanced, so that the C11 has higher alkali resistance, and the application potential of the C11 under an alkaline condition is widened. The cutinase mutant provided by the invention represents a biocatalyst which has a great prospect in application of industrial paper pulp and paper.

This invention discloses a composite microbial preparation for solid fecal degradation, its preparation method, and its application, belonging to the field of microbial preparation technology. The composite microbial preparation comprises a composite microbial agent and an exogenous composite enzyme preparation; the composite microbial agent includes laccase-producing bacteria, keratinase-producing bacteria, auxiliary degradation bacteria, and composite probiotics; the exogenous composite enzyme preparation includes cellulase, neutral protease, and xylanase. This invention employs a mixed strategy of "exogenous enzyme + enzyme-producing bacteria," rapidly decomposing cellulose, protein, and hemicellulose in the initial stage of composting by adding exogenous cellulase, protease, and xylanase, providing readily available carbon and nitrogen sources for the microorganisms, shortening the composting start-up time to less than 24 hours; simultaneously, the laccase-producing bacteria and keratinase-producing bacteria continuously produce laccase and keratinase during composting, achieving deep degradation of lignin and keratin. This invention has advantages such as rapid start-up, continuous degradation, good deodorization effect, high nitrogen retention rate, and low cost, and has good prospects for industrial application.

The invention discloses an acidic serine keratinase gene and application thereof, and belongs to the technical field of gene engineering. The new serine keratinase is screened from the acidic hot spring environment of Yunnan cattle street, has the characteristics of natural acid resistance, good thermal stability and high enzyme activity, and can well adapt to the physiological acidic microenvironment of skin and the temperature fluctuation of the whole process of production, storage and transportation of products; the keratinase is free of complex stabilizing treatment, the production process can be simplified, the production cost can be reduced, the shelf life of products can be prolonged, green and efficient preparation is achieved, the key problems of irritation, poor stability, difficult storage and the like of existing keratinase in skin application are solved from the source, and the keratinase is especially suitable for mild stripping of human body keratosuppository. The invention has outstanding application value and wide prospect in the field of daily chemical skin care.

The invention discloses a method for extracting protein from human hair fibers, and relates to the technical field of application of human hair fibers. The method comprises the following steps: firstly, fully cleaning, drying and cutting human hair fibers into pieces, then putting the human hair fibers into a NaOH solution, treating the human hair fibers in a sterilization pot, filtering the human hair fibers, and washing insoluble substances obtained by filtering with distilled water until the pH value is close to 7; and putting the treated insoluble substance into a mixed solution containing Na2SO3 and urea for treatment, and washing the insoluble substance obtained by filtering after treatment with distilled water until the pH is close to 7. And finally, adding the obtained insoluble substance into a keratinase solution for dissolving, filtering, dialyzing and drying to obtain light yellow human hair keratin powder. The human keratin extracted by the method disclosed by the invention has the advantages of large molecular weight, stable structure and high purity, can be used for manufacturing outer membranes, contents and the like of various human body soft tissue implants or composite implants, can also be prepared into soft tissue injection filling materials, and has an extremely wide application prospect and a very large market development space.

The application relates to the technical field of skin care products, in particular to a preparation method and application of a pH-responsive core-shell composite enzyme composition; carboxymethyl chitosan is dissolved in deionized water, papain and keratinase are added, and stirring is carried out until the mixture is uniform to obtain an enzyme-containing solution; under stirring, the obtained solution is added dropwise into a sodium tripolyphosphate aqueous solution to generate an ionic gelation reaction and form an inner core microsphere loaded with the proteinase; superoxide dismutase is added into the inner core microsphere suspension to obtain a composite enzyme composition suspension; a stabilizer is added into the suspension, and freeze-drying is carried out to obtain a composite enzyme composition powder; the papain and the keratinase in the inner core synergistically decompose aged keratin; the SOD in the outer layer synchronously removes free radicals and relieves inflammatory reactions in the keratin metabolism process; the composition has precise pH response characteristics, can rapidly release active ingredients in the weak alkaline region of acne skin, slowly release the active ingredients in the healthy weak acidic skin region, and realizes targeted repair.

The invention relates to the technical field of pet health care, in particular to a hair removal composition and application thereof. The keratinase and the enterococcus faecium in the hair removal composition have a compound synergistic effect, and after the keratinase softens hair, the keratinase is matched with the enterococcus faecium to effectively promote hair removal and improve the hair removal effect, so that the hair removal composition disclosed by the embodiment of the invention has a good effect of promoting hair removal in animal bodies such as cats and the like; and the occurrence risk of related diseases such as hair bulb is reduced. Besides, through the synergistic effect of keratinase and enterococcus faecium in the hair removal composition, the same hair removal effect can be achieved on the premise that the use dosage of the hair removal composition is reduced, and meanwhile potential side effects and cost are reduced.

The application discloses a low-temperature dyeing and antibacterial shrink-proof composite processing method for wool fabric, and belongs to the technical field of textile dyeing and finishing processing. Firstly, keratinase and protease are used for enzyme shrink-proof treatment of the wool fabric, wool scale keratin is decomposed by enzymolysis, and the wool is endowed with shrink-proof effect; then, low-temperature dyeing treatment is carried out on the wool fabric; finally, glutamine transaminase is used to catalyze amino macromolecules containing glutamine, and bio-enzyme addition composite finishing is carried out, so that the dyeing fastness is improved, the shrink-proof effect is increased, and the wool is endowed with antibacterial property. Compared with traditional chlorination or single enzyme method for wool shrink-proof finishing, the method has the advantages of process ecological environmental protection, low shrinkage rate, low strength loss, excellent antibacterial effect, high color depth and high color fastness.

The invention discloses a cutinase mutant gene, a protein expressed by the cutinase mutant gene and application of the cutinase mutant gene. The protein expressed by the cutinase mutant gene can be correctly folded and can be massively purified in an escherichia coli system. Meanwhile, the mutant protein disclosed by the invention has the function of degrading PET (Polyethylene Terephthalate) by wild cutinase, and the catalytic efficiency of the protein expressed by the cutinase mutant gene is improved compared with that of a control group. The protein expressed by the keratin mutant gene has activity and stability at 40-80 DEG C. Specifically, when the pH value is 8.0 and the temperature is 72 DEG C, the PET degradation capacity is improved by 20% compared with that of a control group, and when the pH value is 8.0 and the temperature is 60 DEG C, the PET degradation capacity is improved by 70% compared with that of the control group.

The invention belongs to the technical field of feed additives, and particularly relates to a compound enzyme for pigs in a fattening period as well as preparation and application thereof. The compound enzyme is prepared by mixing and blending non-starch polysaccharide enzyme (containing arabinofuranosidase), amylase, compound protease (acidic, neutral and alkaline protease and keratinase), a stabilizer and a filler according to a certain proportion. The complex enzyme overcomes the defects of low utilization rate of feed raw materials, high fiber of pig daily ration and low protein utilization rate, especially the participation of arabinofuranosidase and keratinase, fully excavates the potential nutritional value of each feed raw material, opens up a new field for enzyme preparation application, and provides a new idea for cost reduction and efficiency improvement of feed enterprises.