8results about How to "Improve secretion efficiency" patented technology

This invention relates to the field of bioengineering technology, specifically to an endogenous signal peptide element fused with the MF-αpro region and its applications. The endogenous signal peptide element is composed of an endogenous signal peptide from Pichia pastoris with the C-terminus fused with MF-αpro or with amino acids 38 to 51 of the MF-αpro region detached; the amino acid sequence of the MF-αpro region is shown in SEQ ID NO:8; the amino acid sequence of the MF-αpro region with detached amino acids 38 to 51 is shown in SEQ ID NO:10. This invention provides a complete solution from rational screening to functional verification to element optimization. The resulting highly efficient, patent-free signal peptide element is suitable for the production of industrial enzymes, pharmaceutical proteins, and diagnostic reagents.

The invention provides a method for promoting exosome secretion through magnetic stimulation and application of the method, and belongs to the technical field of biological medicine. The method comprises the following steps: S1, preparing magnetic hydrogel; s2, inoculating cells on the magnetic hydrogel; s3, dynamically loading a magnetic field to realize remote magnetic stimulation of the cells; s4, collecting a cell supernatant; and S5, extracting and purifying the exosome. Magnetic force stimulation is applied remotely through a magnetic field, the size of magnetic nanoparticles is extremely small, the generated movement and deformation can be positioned to the subcellular scale, mechanical stimulation can be uniformly and accurately applied to stem cells, the exosome secretion efficiency can be greatly improved, and the exosome secretion efficiency can be improved. Compared with cells cultured on a conventional cell culture plate, the cells on the magnetic hydrogel loaded with the dynamic magnetic field have the advantages that the quantity of generated exosomes is obviously increased and reaches 10 times or more. According to the method, mechanical stimulation on the cells is realized under the non-contact condition, and the method can be applied to various complex cell culture environments.

The application discloses application of a migration body in preparation of a medicine for inhibiting osteoclast differentiation or promoting osteogenesis. The application significantly improves the secretion amount of the migration body by optimizing cell culture conditions, realizes large-scale preparation, and greatly reduces production cost. The application also enriches active ingredients in the migration body by pressure stimulation, so that the migration body has more excellent effect of promoting bone defect repair. In addition, the migration body has larger size and a stable film structure, prolongs in-vivo retention time, realizes long-acting repair by single administration, and further solves the long-acting and clinical transformation problems. The migration body has wide application prospect in the field of bone defect repair.

This invention discloses a method and application for rapidly preparing humanized collagen using umbilical cord mesenchymal stem cell material, belonging to the field of biomaterial preparation technology. The key technical points of the method are as follows: S1, processing and passage-expanding umbilical cord tissue; S2, seeding the expanded umbilical cord mesenchymal stem cell suspension into a culture dish, then adding DMEM / F12 medium containing platelet lysis buffer for culture, followed by induction medium to obtain extracellular matrix; S3, enzymatically hydrolyzing the extracellular matrix, centrifuging the hydrolysate to obtain the supernatant, adjusting the pH, allowing it to stand, centrifuging again to obtain the precipitate, reconstituteing the precipitate, purifying it, centrifuging again, and then lyophilizing it; S4, forming the formulation to achieve efficient secretion and self-assembly of collagen, while utilizing extracellular matrix components (such as fibronectin and laminin) secreted by umbilical cord mesenchymal stem cells to assist collagen in forming an active product with a natural triple helix structure.

The application discloses an ovarian anti-aging cell biological preparation and a preparation method and application thereof, and belongs to the technical field of biotechnology. The biological preparation is obtained by compounding ovarian stem cells and mulberry source specific polypeptides SEQ ID NO. 1 and SEQ ID NO. 2, a double mechanism of cell repair and molecular regulation is formed, animal experiments show that the efficiency of promoting E2 secretion and inhibiting FSH secretion is improved, and the follicle survival rate under a microscope is significantly improved, and the ovarian anti-aging aspect embodies a good treatment prospect. Meanwhile, the application adopts the medicinal and edible mulberry to replace the active ingredients extracted from radix falcaliae polygoni multiflori, and avoids the hepatotoxicity risk.

A method for increasing the secretion rate of foreign proteins expressed in silkworm cells, extracting cDNA obtained by reverse transcription of all RNA from silkworm BmN cells, and synthesizing the cDNA using the reverse transcribed cDNA as a template license plate Gene, then the SEQ ID NO. license plate Gene transfer into silkworm BmN cells can increase the secretion rate of foreign proteins expressed in silkworm cells. The present invention can effectively increase the secretion efficiency of foreign proteins expressed in silkworm cells, provide new research directions and ideas for increasing the secretion rate of foreign proteins expressed in silkworm cells, and provide a reference for the study of the mechanism of signal peptides involved in protein secretion.

The present invention relates to a fusion protein, an immunogenic composition, a recombinant vaccine, and a molecular architecture design and application for preventing pneumococcal infection. The present invention starts from the tertiary structure of the protein molecules of ply and PhtD, and after creative screening, finally selects the C-terminal domain of the ply protein and the N-terminal domain of the phtD protein to construct a fusion protein, and then further adds elements such as the Fc domain and STABILON. The fusion protein molecule of the present invention can weaken tissue lesions caused by pneumococcal infection, has good immunogenicity, plays an effective preventive and immune protection role, and effectively prevents pneumococcal infection. The immunogenic composition, fusion protein, and recombinant vaccine for preventing pneumococcal infection of the present invention have broad application prospects.

The application discloses a breast pump, which comprises a main machine and a breast cup. The main machine comprises a face shell and a massage assembly, and the massage assembly is arranged on the face shell. The breast cup comprises a first cup body and a massage part. The first cup body is arranged on the surface of the face shell and detachably connected with the face shell. The massage part is arranged on the first cup body and protrudes from the first cup body. The massage assembly abuts against the protruding part of the massage part to massage the human body through the massage part. In the example of the application, the massage part is arranged on the breast cup, so that the breast can be massaged during use to reduce the pain of the breast during milk swelling, and the comfort during the breast pumping process is improved, and the breast milk secretion efficiency is improved.