44 results about "Rhizomucor miehei" patented technology

Topical compositions comprising extracts of Rhizomucor miehei that are substantially devoid of acid-protease activity and their use in treating dermatologic conditions, including reducing the appearance of signs of skin aging.

Methods and compositions for reducing and / or inhibiting inflammation by topical application of dermatocosmetic compositions comprising effective amounts of extracts of Coriolus versicolor that have been hydrolyzed by an acid protease, preferably Rhizomucor miehei, and thereafter rendered substantially devoid of acid-protease activity.

The invention relates to a sulfur-containing flavor ester composition and a preparation method and cheese essence thereof. The preparation method comprises the following steps: (1) taking anhydrous milk fat and 3-methylthio-propanol as raw materials, and adding 200-400U of rhizomucor miehei lipase into the raw materials per gram for performing enzymatic hydrolysis, thereby obtaining an enzymolysis product; (2) performing level-I molecular distillation on the enzymolysis product obtained in the step (1), wherein the vacuum degree of level-I molecular distillation is 30-40Pa, removing the fraction on a temperature section of 50 DEG C or below, and continuously performing level-II molecular distillation, wherein the vacuum degree of level-II molecular distillation is less than 5Pa, collecting a light phase part of the fraction on a temperature section of 50-180 DEG C, thereby obtaining the product. According to the invention, the preparation method is simple, and the prepared sulfur-containing flavor ester composition has aroma of cheese and cream cheese after high-temperature processing. The cheese essence prepared by taking the sulfur-containing flavor ester composition disclosed by the invention as a raw material is strong in flavor, mellow, natural and good in high-temperature stability.

The invention provides a chiral resolution method of an isobutyrate compound. The isobutyrate compound is a racemic isobutyrate compound, and has the following structural general formula: R1 is selected from any one of the following substituted or unsubstituted groups: pyridine, pyrazole, pyrimidone, 4-hydroxyquinoline, quinazoline and quinazolinone, R2 is selected from substituted or unsubstituted alkyl and aryl, and the chiral resolution method comprises the following steps: S1, carrying out hydrolysis treatment on a racemic isobutyrate compound by adopting hydrolase to obtain a hydrolyzed system, wherein the hydrolase is derived from Thermomyces sp.Rhizomucor miehei, Bacillus sp.; and S2, separating the acid in the hydrolyzed system from the isobutyrate compound which is not hydrolyzed. The hydrolase used in the method has the advantages of good stability, good enantiomer selectivity, mild reaction conditions, low cost and simple operation.

The invention provides a method for fermenting chymosin by using nisin fermentation wastewater as a partial nitrogen source, relating to the method for producing the chymosin by microbial fermentation. The invention solves the problems that at current, the method for producing the chymosin by the microbial fermentation has high cost, and the direct discharge of the fermentation wastewater of the nisin results in environment pollution. The method comprises the following steps: 1, preparing a culture medium; 2, preparing rhizomucor miehei seed liquor; 3, inoculating the rhizomucor miehei seed liquor to a triangular flask; and 4, culturing the inoculated seed liquor for 96h at the temperature of 37-38 DEG C to obtain the chymosin. The invention can utilize waste, reduce the environment pollution caused by directly discharging the fermentation wastewater of the nisin, and lower the cost for fermenting the chymosin. The invention can be applied to the field of microbial fermentation.