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34 results about "Thermomyces lanuginosus" patented technology
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High-temperature cow dung decomposition agent and preparation method thereof
InactiveCN105154373APromote growthGrowth inhibitionBio-organic fraction processingFungiBacillus licheniformisThermomyces lanuginosus
The invention discloses a high-temperature cow dung decomposition agent which is prepared from cow dung and a compound microbial agent through fermentation and drying, wherein cow dung particles in the high-temperature cow dung decomposition agent are loaded with compound bacteria of bacillus subtilis, bacillus licheniformis, in-situ composting geobacillus toebii, thermomyces lanuginosus and thermobifida fusca. In addition, the living bacteria count is not lower than 48+ / - 100 millions / g, the high-temperature cow dung decomposition agent is 50-650 [mu]m in average particle size, lower than 15% in moisture content and 6.0-8.0 in pH. The compound microbial agent in the decomposition agent generates plenty of cellulase, hemicellulase and protease, so that cow dung is rapidly decomposed into a simple inorganic nitrogen source and a carbon source, further, plant growth is promoted and soil fertility is improved. Fermentation is carried out under the high temperature environment, so that mesophilic plant pathogen growth is inhibited effectively, and cow dung products fermented through the decomposition agent can be directly used as organic fertilizer to be applied to soil.
Owner:SHANDONG UNIV
Pichia yeast engineering strain for expressing Thermomyces lanuginosus gene 1n
The invention relates to a Pichia pastoris GS-LN-6 for expressing a Thermomyces lanuginosus thermo-stabilization lipase gene ln. The lipase gene ln is obtained from the Thermomyces lanuginosus through a method of RT-PCR, and is cloned and inserted into a Pichia pastoris integral expression vector pPIC9K. Then the expression vector pPIC9K / ln of the obtained lipase gene ln is inducted into a Pichia pastoris GS115 from which the Pichia pastoris GS-LN-6 for expressing the lipase is screened out. The engineering bacteria can have a lipase expression amount of 1.93mg / mL after undergoing six days of induction culture. The enzyme still has 82 percent of activity when the temperature is kept at 60 DEG C for 60 minutes, has 50 percent of activity when the temperature is kept at 70 DEG C for 60 minutes, and has 45 percent of activity when the temperature is kept at 80 DEG C for 60 minutes. The Pichia pastoris GS-LN-6 can be used as a production strain of the thermo-stabilization lipase with thermal stability and higher economic and social value.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Organic fertilizer fermentation inoculant and application
The invention discloses an organic fertilizer fermentation inoculant and application. The inoculant is composed of a primary fermentation inoculant body and a secondary fermentation inoculant body. The primary fermentation inoculant body comprises bacillus subtilis, bacillus licheniformis, trichoderma viride, aspergillus oryzae, pseudomonas, trichoderma koningii, geotrichum candidum, long spiny shells, ascomycetes, thermomyces lanuginosus and thermobifida fusca. The secondary fermentation inoculant body comprises halophilic bacteria, alkalophilic bacteria, azotobacter chroococcum, bacillus megatherium, streptomyces griseus, bacillus thuringiensis, brevibacillus laterosporus and bacillus mucilaginosus. Time for fermenting organic fertilizer with cow dung as the raw material can be effectively shortened, and diseases and insect pests in the fertilizer can be eliminated. Meanwhile, alkalophilic bacteria and bacillus thuringiensis are added so that the fermentation inoculant can adapt to saline-alkali soil and can prevent diseases and insect pests of plants, and the prepared organic fertilizer can improve the soil structure, reduces the salt content and the pH value in soil, improves soil fertility and is particularly beneficial to improvement of saline-alkali soil.
Owner:SHANDONG SUNWAY LANDSCAPE TECH
Maize straw high-temperature decomposition agent and preparing method thereof
InactiveCN105254353APromote growthGrowth inhibitionBio-organic fraction processingOrganic fertiliser preparationCelluloseBacillus licheniformis
The invention discloses a maize straw high-temperature decomposition agent. The maize straw high-temperature decomposition agent is prepared by fermenting and then drying maize straw powder and complex microbial inoculants. The maize straw powder particles in the maize straw high-temperature decomposition agent are loaded with a bacterium mixture containing thermomyces lanuginosus, thermobifida fusca, thermopolyspora flexuosa, streptomyces thermoviolaceus, in-situ composting bacillus licheniformis and bacillus subtilis. Living bacteria count is not lower than 48+ / -1 hundred million / g. The average grain diameter of the maize straw high-temperature decomposition agent is 150-650 micrometers, water content is lower than 15%, and pH is 6.0-8.0. By the adoption of the decomposition agent, the lignocelluloses substance and the protein substance in maize straw can be degraded quickly to achieve straw decomposing, and rich simple inorganic carbon sources and nitrogen sources are generated. Decomposed maize straw is made into organic fertilizer to effectively promote the growth of plants and improve the fertility of soil. Soil improvement and environment management are facilitated, and sustainable development of agriculture is achieved.
Owner:SHANDONG UNIV
Thermomyces lanuginosus lipase gene, engineering bacteria and application of engineering bacteria
ActiveCN105087614AImprove stabilityPromote accumulationFungiHydrolasesThermomyces lanuginosusProtein structure
The invention discloses a thermomyces lanuginosus lipase gene, engineering bacteria and an application of the engineering bacteria. The base sequence of the thermomyces lanuginosus lipase gene is shown in SEQ ID NO.2. On the basis that existing thermomyces lanuginosus lipase amino acid sequences and protein structures are not changed, factors adverse to protein expression, for example, rare codons, rare codon pairs, strong stem-loop structures, potential early termination signals and the like are avoided, and the thermomyces lanuginosus lipase gene sequence is optimized, so that the sequence can efficiently express thermomyces lanuginosus lipase in a pichia pastoris recombination expression system, and the expression vitality is up to 370.65 U / ml and is improved by 58.38% while compared with the recombination expression vitality of the original sequence of the thermomyces lanuginosus lipase gene being 234.03 U / ml.
Owner:ZHEJIANG UNIV
Method for immobilizing Thermomyces lanuginosus lipase
InactiveCN107012136ASimple preparation conditionsEasy to operateOn/in organic carrierCross-linkOrganic solvent
The invention discloses a method for immobilizing Thermomyces lanuginosus lipase. The method comprises the following steps: adding 100 L of an enzyme liquid of Thermomyces lanuginosus lipase Lipozyme TL into an organic solvent at 4 DEG C, carrying out standing precipitation for 1 h, centrifuging the obtained solution, taking the obtained supernatant, adding a cross-linking agent and macro-porous adsorption resin, carrying out oscillation cross-linking immobilization, and carrying out vacuum drying after the above reaction is completed in order to obtain immobilized Thermomyces lanuginosus lipase. The immobilized lipase has the advantages of simple preparation conditions, and convenience in operation. The enzyme activity and the batch stability of the immobilized Thermomyces lanuginosus lipase are much higher than those of the commercialized enzyme Lipozyme TL IM, so the method is suitable for industrial application.
Owner:ZHEJIANG UNIV OF TECH +1
Method and application for increasing oligomerization wood sugar content in black tea
InactiveCN103300177AEasy to operateEasy to controlPre-extraction tea treatmentThermomyces lanuginosusAnimal testing
The invention provides a method for increasing the oligomerization wood sugar content in black tea aiming at the disadvantages that the oligomerization wood sugar content in the black tea at present is low and the effect is inadequately obvious. The method is characterized in that heat-resistant xylanase is produced in the black tea fermentation process through inoculating a xylanase producing strain, namely Thermomyces lanuginosus, and thus oligomerization wood sugar produced by xylan in the black tea is degraded; detection results prove that compared with the traditional black tea, the oligomerization wood sugar content in the black tea prepared by using the method is increased by 20-40 times; the animal experiment results prove that bifidobactirium proliferation in the intestinal tracts of white rats fed by the black tea is obvious and is improved by about 8% compared with that in the intestinal tracts of the white rats fed by the traditional feeding black tea, and the growth of enteric bacilli, enterococcus and the like can be inhibited to different degrees.
Owner:湖南惟楚福瑞达生物科技有限公司
Preparation method and application of thermomyces lanuginosus lipase
ActiveCN102604913AThe whiteness improvement effect is obviousHydrolasesPre-baking dough treatmentThermomyces lanuginosusTrichoderma reesei
The invention discloses a preparation method of a thermomyces lanuginosus lipase. The method comprises the following steps of: 1) synthesizing a thermomyces lanuginosus lipase gene; 2) constructing a thermomyces lanuginosus lipase expression plasmid; 3) converting a trichodermareesei and selecting high-yield strains of the thermomyces lanuginosus lipase; and 4) separating and purifying the thermomyces lanuginosus lipase. The invention also discloses the application of the thermomyces lanuginosus lipase to the preparation of flour foods.
Owner:SHANDONG YOUTELL BIOCHEM
Garden waste degradation bacteria agent
InactiveCN108486017AImprove degradation rateImprove degradation efficiencyFungiBacteriaEcological environmentThermomyces lanuginosus
The invention provides a garden waste degradation bacteria agent. The garden waste degradation bacteria agent comprises a bacteria groupand a basic culture solution, wherein the bacteria group comprises Promicromonospora, Burkholderia, Thermomyces lanuginosus, Shigella and Streptomyces; the basic culture solution comprises wheat bran, starch, xylose, beef extract, ammonium nitrate and sterile water; the weight ratio of the bacteria group to the basic culture solution is 1:1.5-1:2. The garden waste degradation bacteria agent has the advantages that after fermentation of garden wastes is finished by using the degradation bacteria agent, the degradation rates of hemicellulose, cellulose and lignin are obviously higher than those obtained after one kind of bacteria is individually used; the Shigella and the Streptomyces are degraded by adding sugar, other polysaccharides in the garden wastes can be efficiently degraded, and the overall fermentation process is improved. The compound degradation bacteria agent is high in lignin degradation efficiency; land resources are saved, soil and the ecological environment are improved, environmental pollution is mitigated, the substrate obtained after degradation can be turned into treasure, and resource recycling is achieved.
Owner:SUZHOU UNIV
Method for enriching long-chain polyunsaturated fatty acid through immobilized thermomyces lanuginosus
ActiveCN104130860AReduce usageSimple processFatty-oils/fats separationFermentationMicrobial oilThermomyces lanuginosus
The invention relates to a method for enriching long-chain polyunsaturated fatty acids through immobilized thermomyces lanuginosus. The method includes following steps: (1) carrying out an esterification reaction to a free fatty acid and an alcohol in the presence of the immobilized thermomyces lanuginosus in vacuum or a protective atmosphere; and (2) separating free long-chain polyunsaturated fatty acids out from a product after the reaction. By means of selectively catalytic esterification to fatty acids with different chain lengths during a catalytic esterification process of the fatty acid and the alcohol through immobilized lipases, a new method for enriching the long-chain polyunsaturated fatty acids from deep sea fish oil and microbial oil is achieved.
Owner:WILMAR SHANGHAI BIOTECH RES & DEV CENT
Thermomyces lanuginosus lipase mutant, coding genes, and applications of thermomyces lanuginosus lipase mutant
ActiveCN103981160AIncreased parental vitalityHydrolasesFermentationThermomyces lanuginosusPeptide fragment
The invention provides a thermomyces lanuginosus lipase mutant, coding genes, and applications of the thermomyces lanuginosus lipase mutant. The thermomyces lanuginosus lipase mutant is obtained via point mutation of a peptide fragment with a sequence represented by SEQ ID No.1; and the point mutation is one selected from the following steps or a combination of the following steps: (1) S at the 63th site is changed into L or M; and (2) D at the 232th site is changed into A. Compared with the parent, vitality of the thermomyces lanuginosus lipase mutant is increased greatly; the thermomyces lanuginosus lipase mutant can be used for industrialized production of (3S)-2-carboxyethyl-3-cyano-5-methylhexanol, and further synthesis of pregabalin.
Owner:ZHEJIANG UNIV OF TECH
Composite microbial inoculum for degradation of livestock and poultry manure position and application of composite microbial inoculum
ActiveCN111334452AFully degradedImprove efficiencyBio-organic fraction processingFungiBiotechnologyThermomyces lanuginosus
The invention provides a composite microbial inoculum for degradation of livestock and poultry manure position and an application of the composite microbial inoculum. The composite microbial inoculumconsists of thermomyces lanuginosus NJAU-F4-11 bacterial liquid, bacillus NJAU-N45 bacterial liquid, bacillus NJAU-ND8 bacterial liquid and bacillus B5 bacterial liquid. The application of the composite microbial inoculum is to accelerate compost fermentation. The application comprises the following steps: uniformly mixing livestock and poultry manure and auxiliary materials according to a certainC / N ratio, inoculating the mixed livestock and poultry manure and auxiliary materials with a liquid strain, piling the inoculated poultry manure and auxiliary materials into strip piles, and performing turning fermentation by adopting a strip pile type fermentation process. Compared with control groups without adding strains and control groups added with single strains, the addition of the composite microbial inoculum effectively promotes increase of the temperature of the piles at the initial stage of composting, promotes the piles to enter a cooling period in advance, and promotes improvement of the germination index and the like of the composting products.
Owner:NANJING AGRICULTURAL UNIVERSITY
Bacteria preparation for processing waste with high lignin content
ActiveCN112625921AImprove composting efficiencyBio-organic fraction processingFungiBiotechnologyMicroorganism
The invention belongs to the field of microorganisms, and specifically relates to a bacteria preparation for processing waste with high lignin content. The specific technical schemes are as follows: thermomyces lanuginosus is deposited with the China General Microbiological Culture Collection Center on December 1st, 2020 and assigned accession number CGMCC No. 21075. The provided bacteria preparation includes the thermomyces lanuginosus and sordaria. Two novel microorganisms capable of degrading lignin are provided, have the capacity of independently degrading the lignin, and have a certain capacity of degrading cellulose and hemicellulose. Through the preparation of the two microorganisms into the bacteria preparation used for composting wastes with high lignin content, the effects of greatly enhancing composting efficiency can be achieved by only using the two microorganisms; and therefore, the bacteria preparation has wide application prospects on lignin processing.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Lipase mutant D163F with improved catalytic activity and application thereof
Owner:YUNNAN NORMAL UNIV
Method for preparing polyesters by enzyme catalysis
The invention discloses a method for preparing the polyester through the enzyme catalysis. In the method, a reaction is carried out so as to prepare the polyester under a very gentle conditions with the Beta-hydroxy carboxylic ester as reactant and enyzymes Novozyme435, Lipase from thermomyces lanuginosus PPL or Lipase PS as catalysts. The invention is a solvent-free reaction system, has a high ezyme stability and can be used for carrying out continuous catalytic reactions for many times, and the chemical monomers which can go to scale and the engineering enzyme are adopted as catalysts in the polymerization, thus greatly lowering the cost of the product.
Owner:LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Mutated Aspergillus oryzae strain
The present invention relates to a mutated Aspergillus oryzae strain. Specifically, the invention relates to a mutated Aspergillus oryzae strain of which the productivity is improved by being compared with a non-mutated strain, endogenous enzymes, such as amylase and / or foreign protein, and food-purpose lipase, preferably the food-purpose lipase selected from rhizomucor miehei lipase, thermomyces lanuginosus lipase and fusarium oxysporum lipase. The invention also relates to various applications of the strain.
Owner:WILMAR SHANGHAI BIOTECH RES & DEV CENT
Method for preparing oligosaccharides with side chains by utilizing thermophilic filamentous fungus fermentation one-step method
InactiveCN108048506ALow costReduce the cost of separation and purificationMicroorganism based processesFermentationThermomyces lanuginosusSide chain
The invention discloses a method for preparing oligosaccharides with side chains by utilizing a thermophilic filamentous fungus fermentation one-step method. The method is characterized in that the oligosaccharides with the side chains are prepared by utilizing a method for solid fermentation or liquid fermentation of thermomyces lanuginosus. An experiment verifies that through the thermophilic properties of the thermomyces lanuginosus, fermentation conditions are easy to control in the production process of the thermomyces lanuginosus; meanwhile, the thermomyces lanuginosus can utilize cheapcorn cobs, bran and other agricultural and sideline products to ferment and produce the oligosaccharides with the arabinose side chains; the invention disclosed by the invention is a sustainable production method, can reduce the raw material cost and the separation and purification cost and is an environment-friendly and efficient production process.
Owner:SHANDONG UNIV
Thermomyces lanuginosus lipase mutant G91C and application thereof
ActiveCN113846074AHigh catalytic activityIncrease vitalityHydrolasesBiofuelsThermomyces lanuginosusMutant
The invention provides a thermomyces lanuginosus lipase mutant G91C and application thereof. The application comprises the following steps: predicting a mutation site, preparing a lipase mutant, determining the enzyme activity of lipase, and testing thermal stability and pH stability. The preparation method provided by the invention can realize lipase with higher enzyme activity and better thermal stability.
Owner:NANJING FORESTRY UNIV
Esterase, gene, vector and engineering bacterium derived from thermomyces lanuginosus as well as application
The invention discloses an esterase, a gene, a vector and an engineering bacterium derived from thermomyces lanuginosus as well as application in preparing (3S)-2-carboxyethyl-3-cyan-5-methyl hexanoic acid. An amino acid sequence of the esterase is represented in SEQ ID NO: 2. The esterase gene disclosed by the invention can be linked to an expression vector to construct an intracellular expression recombinant plasmid containing the gene and the intracellular expression recombinant plasmid is transformed to an escherichia coli strain, thus obtaining recombinant escherichia coli. The recombinant escherichia coli contains the esterase; the recombinant escherichia coli or purified esterase is applied to biological catalysis as a catalyst to produce a pregabalin key chiral intermediate.
Owner:ZHEJIANG UNIV OF TECH
Lipase-calcium phosphate compound enzyme crystal, preparation method thereof and method for catalyzing and synthesizing clindamycin palmitate by using lipase-calcium phosphate compound enzyme crystal
ActiveCN108342433AReduce pollutionMild reaction conditionsHydrolasesPeptidesCalcium biphosphateThermomyces lanuginosus
The invention provides a lipase-calcium phosphate compound enzyme crystal, a preparation method thereof and a method for catalyzing and synthesizing clindamycin palmitate by using the lipase-calcium phosphate compound enzyme crystal, and belongs to the technical field of antibiotic drug synthesis. According to the method, clindamycin free alkali shown in the formula I and ethenyl hexadecanoate shown in the formula II serve as a substrate and react with a reaction solvent for 6-30 h at the temperature of 30-80 DEG C under the catalytic function of the lipase-calcium phosphate compound enzyme crystal, and clindamycin palmitate shown in the formula III is prepared. The lipase-calcium phosphate compound enzyme crystal prepared through the method is a calcium phosphate crystal of thermomyces lanuginosus lipase. The lipase-calcium phosphate compound enzyme crystal has the advantages that the cost of the method is low, immobilized enzyme cannot be dissolved in an organic solvent and can be recycled, the yield can reach 75.8% at most, the method is safe, reliable, low in environment pollution and suitable for industrial production.
Owner:HANGZHOU NORMAL UNIVERSITY
Lipase composition and application thereof
PendingCN109943551AReduce manufacturing costSpeed up the initial responseHydrolasesAccessory food factorsChemical industryThermomyces lanuginosus
The invention discloses a lipase composition and application thereof, and belongs to the technical field of enzyme engineering. The lipase composition is composed of 25-50% of lipase from pseudomonasaeruginosa and 50-75% of lipase from thermomyces lanuginosus according to the protein content. The lipase composition has the synergic effect for a hydrolysis reaction and an esterification reaction for catalyzing grease. Triglyceride can be rapidly hydrolyzed, the esterification initial reaction speed is increased, the reaction process is more gentle, fluctuations are little, when the reaction isended, more esterification products can be generated, the enzymic preparation dosage is lowered, the reaction time is effectively shortened, the production cost is effectively saved, and the excellent application and popularization value is achieved in the fields of grease chemical industry, feed additives, detergents and the like.
Owner:BEIJING INST OF TECH ZHUHAI CAMPUS
Method for Enriching Long-chain Polyunsaturated Fatty Acids Using Immobilized Thermomyces lanuginosus Lipase
ActiveCN104130860BReduce usageSimple processFatty-oils/fats separationFermentationMicrobial oilThermomyces lanuginosus
The invention relates to a method for enriching long-chain polyunsaturated fatty acids through immobilized thermomyces lanuginosus. The method includes following steps: (1) carrying out an esterification reaction to a free fatty acid and an alcohol in the presence of the immobilized thermomyces lanuginosus in vacuum or a protective atmosphere; and (2) separating free long-chain polyunsaturated fatty acids out from a product after the reaction. By means of selectively catalytic esterification to fatty acids with different chain lengths during a catalytic esterification process of the fatty acid and the alcohol through immobilized lipases, a new method for enriching the long-chain polyunsaturated fatty acids from deep sea fish oil and microbial oil is achieved.
Owner:WILMAR SHANGHAI BIOTECH RES & DEV CENT
Thermomyces lanuginosa lipase mutants, encoding genes and their applications
ActiveCN103981160BIncreased parental vitalityHydrolasesGenetic engineeringThermomyces lanuginosusVitality
The invention provides a thermomyces lanuginosus lipase mutant, coding genes, and applications of the thermomyces lanuginosus lipase mutant. The thermomyces lanuginosus lipase mutant is obtained via point mutation of a peptide fragment with a sequence represented by SEQ ID No.1; and the point mutation is one selected from the following steps or a combination of the following steps: (1) S at the 63th site is changed into L or M; and (2) D at the 232th site is changed into A. Compared with the parent, vitality of the thermomyces lanuginosus lipase mutant is increased greatly; the thermomyces lanuginosus lipase mutant can be used for industrialized production of (3S)-2-carboxyethyl-3-cyano-5-methylhexanol, and further synthesis of pregabalin.
Owner:ZHEJIANG UNIV OF TECH
A lipase mutant with improved catalytic activity and its application
The invention belongs to the technical field of enzyme engineering, and discloses a lipase mutant with improved catalytic activity and its application. The amino acid sequence of the lipase mutant is as SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO. 4. As shown in SEQ ID NO.5, the lipase mutant changes from aspartic acid at position 116 to lysine and aspartic acid at position 163 in the amino acid sequence SEQ ID NO.1 encoding Thermomyces lanuginosus lipase Aspartic acid is changed to phenylalanine, aspartic acid at position 170 is changed to alanine, aspartic acid at position 259 is changed to tyrosine, and its coding genes are as SEQ ID NO.6, SEQ ID NO. 7. Shown in SEQ ID NO.8 and SEQ ID NO.9. The catalytic activity of the four mutants was improved.
Owner:YUNNAN NORMAL UNIV
Application of Thermophilic Bacteria in Improving Fertility, Potassium and Magnesium Fertilizer Content
The invention relates to application of thermophilic bacteria in fertility improvement and potassium fertilizer and magnesium fertilizer content increase. The thermophilic bacteria is thermomyces dupontii and / or thermomyces lanuginosus.
Owner:YUNNAN UNIV
Application of thermophilic bacteria in fertility improvement and potassium fertilizer and magnesium fertilizer content increase
ActiveCN107827486AColor bright greenImprove fertilityOrganic fertilisersThermusThermomyces lanuginosus
The invention relates to application of thermophilic bacteria in fertility improvement and potassium fertilizer and magnesium fertilizer content increase. The thermophilic bacteria is thermomyces dupontii and / or thermomyces lanuginosus.
Owner:YUNNAN UNIV
Thermomyces lanuginosa lipase gene, engineering bacteria and its application
ActiveCN105087614BOptimize gene sequenceEfficient expressionFungiHydrolasesPichia pastorisThermomyces lanuginosus
The invention discloses a thermomyces lanuginosus lipase gene, engineering bacteria and an application of the engineering bacteria. The base sequence of the thermomyces lanuginosus lipase gene is shown in SEQ ID NO.2. On the basis that existing thermomyces lanuginosus lipase amino acid sequences and protein structures are not changed, factors adverse to protein expression, for example, rare codons, rare codon pairs, strong stem-loop structures, potential early termination signals and the like are avoided, and the thermomyces lanuginosus lipase gene sequence is optimized, so that the sequence can efficiently express thermomyces lanuginosus lipase in a pichia pastoris recombination expression system, and the expression vitality is up to 370.65 U / ml and is improved by 58.38% while compared with the recombination expression vitality of the original sequence of the thermomyces lanuginosus lipase gene being 234.03 U / ml.
Owner:ZHEJIANG UNIV
A lipase-calcium phosphate complex enzyme crystal, its preparation method and its catalytic method for synthesizing clindamycin palmitate
ActiveCN108342433BReduce pollutionMild reaction conditionsHydrolasesPeptidesThermomyces lanuginosusCatalytic function
Owner:HANGZHOU NORMAL UNIVERSITY
Esters, genes, vectors, engineering bacteria and applications derived from Thermomyces lanuginosus
The invention discloses an esterase, a gene, a vector and an engineering bacterium derived from thermomyces lanuginosus as well as application in preparing (3S)-2-carboxyethyl-3-cyan-5-methyl hexanoic acid. An amino acid sequence of the esterase is represented in SEQ ID NO: 2. The esterase gene disclosed by the invention can be linked to an expression vector to construct an intracellular expression recombinant plasmid containing the gene and the intracellular expression recombinant plasmid is transformed to an escherichia coli strain, thus obtaining recombinant escherichia coli. The recombinant escherichia coli contains the esterase; the recombinant escherichia coli or purified esterase is applied to biological catalysis as a catalyst to produce a pregabalin key chiral intermediate.
Owner:ZHEJIANG UNIV OF TECH
Thermomyces lanuginosus lipase mutant and coding gene
PendingCN114317490AMutation results are safeEnzyme activityHydrolasesGenetic engineeringThermomyces lanuginosusSite-directed mutagenesis
The invention discloses a thermomyces lanuginosus lipase mutant and a coding gene. The amino acid sequence of the mutant is shown as SEQ ID NO: 2. According to the mutant disclosed by the invention, polar amino acid at a specific position is mutated into non-polar amino acid, site-directed mutagenesis molecular modification of rational design is carried out on existing enzyme molecules, the mutated enzyme molecular structure is not destructively changed, a mutation result is relatively safe, and enough enzyme activity can be ensured or higher enzyme activity can be obtained.
Owner:NANTONG CHANGYOO PHARMATECH CO LTD
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