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Lipase composition and application thereof

A technology of lipase and composition, which is applied in the field of enzyme engineering, can solve problems such as few structure and catalytic substrate affinity and activity, achieve good promotion and application value, speed up the initial reaction speed, and smooth the reaction process

Pending Publication Date: 2019-06-28
BEIJING INST OF TECH ZHUHAI CAMPUS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few literatures and patents on lipase compatibility, and they are relatively simple. They are often compounded based on the most basic optimum pH, optimum temperature, etc., and seldom start from the compatibility and activity of their structure and catalytic substrate.

Method used

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  • Lipase composition and application thereof

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preparation example Construction

[0039] The present invention also provides a kind of preparation method of fatty acid methyl ester, it is to use lipase composition as above to prepare fatty acid methyl ester, and concrete steps are as follows:

[0040] 1) Take kitchen waste grease or deodorized vegetable oil distillate and place it in the reaction device, add lye, adjust the pH to 5-6.5, and stir evenly at 30-45°C for later use; wherein, the lye is 0-560ppm NaOH;

[0041] 2) Weighing 0.1% to 0.6% lipase composition according to the mass ratio, adding it to purified water or tap water, mixing evenly, and then adding it to the soybean oil deodorized distillate in step 1), and continuing the reaction;

[0042] 3) During the reaction process, add 1.5-2 equivalents of methanol several times, and continue to stir and react at a speed of 10-300r / min for 2-24h. The oil sample is separated into layers, the oil sample is taken, and the fatty acid methyl ester is obtained.

Embodiment 1

[0044] A lipase composition, the lipase composition is composed of the following components in mass percentage: 75% of lipase from Pseudomonas aeruginosa, 25% of lipase from Thermomyces lanuginosa.

[0045] A kind of preparation method of fatty acid methyl ester, it is to use lipase composition as above to prepare fatty acid methyl ester, concrete steps are as follows:

[0046] 1) Take kitchen waste oil and put it in a triangular flask, add 240-560ppm lye NaOH to adjust the pH to 5-6, shake well in a shaker at a temperature of 30°C for later use;

[0047] 2) Take a lipase composition with a mass ratio of 0.3% and add it into purified water, mix evenly, then add it to the soybean oil deodorized distillate in step 1), and place it in a shaking table for reaction;

[0048] 3) Add 1.5 equivalents of methanol several times 3 hours before the reaction, centrifuge at a speed of 300r / min for 24 hours, add 0.25 equivalents of methanol at 0 hours, add 0.25 equivalents of methanol at 1 h...

Embodiment 2

[0050] A lipase composition, the lipase composition is composed of the following components in terms of protein mass percentage: 50% of lipase from Pseudomonas aeruginosa, 50% of lipase from Thermomyces lanuginosa.

[0051] A kind of preparation method of fatty acid methyl ester, it is to use lipase composition as above to prepare fatty acid methyl ester, concrete steps are as follows:

[0052] 1) Take the deodorized distillate of soybean oil and place it in a reaction kettle with a volume of 6 cubic meters and add 200-300ppm of lye NaOH to adjust the pH to 5-6. spare;

[0053] 2) Take a lipase composition with a mass ratio of 0.2% and add it to tap water, mix evenly, then add it to the soybean oil deodorization distillate in step 1), adjust the moisture content of the entire reaction system to 3%, and place it in a reaction kettle reaction;

[0054] 3) 1.5 equivalents of methanol was added during the reaction for 8 hours, and the reaction was centrifuged at a speed of 50 r / ...

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Abstract

The invention discloses a lipase composition and application thereof, and belongs to the technical field of enzyme engineering. The lipase composition is composed of 25-50% of lipase from pseudomonasaeruginosa and 50-75% of lipase from thermomyces lanuginosus according to the protein content. The lipase composition has the synergic effect for a hydrolysis reaction and an esterification reaction for catalyzing grease. Triglyceride can be rapidly hydrolyzed, the esterification initial reaction speed is increased, the reaction process is more gentle, fluctuations are little, when the reaction isended, more esterification products can be generated, the enzymic preparation dosage is lowered, the reaction time is effectively shortened, the production cost is effectively saved, and the excellent application and popularization value is achieved in the fields of grease chemical industry, feed additives, detergents and the like.

Description

technical field [0001] The invention relates to the technical field of enzyme engineering, in particular to a lipase composition and its application. Background technique [0002] Enzymes, as biocatalysts, are highly specific and specific. The essence of an enzyme preparation is a protein, and its catalytic function and specificity are determined by the high-level structure of the protein. Proteins composed of long-chain amino acids have different polarities due to the different groups of different amino acid residues, for example, due to intermolecular hydrogen bonds, van der Waals forces, ionic forces, and sometimes covalent bonds such as disulfide bonds. After being folded and modified in the cell, under the joint action of these intermolecular forces, a functional higher-level structure is finally formed. The folded enzyme protein usually has at least one active center that catalyzes the reaction and a binding structure that captures the reaction substrate. The bindin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C12P7/62C11D3/386A23K20/189
Inventor 韩望黄友雪黄大鑫曹议元罗冠聪曹弘隽林小欣奉心茹甘露菁金铁铨
Owner BEIJING INST OF TECH ZHUHAI CAMPUS
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