The invention relates to an integrated micro-fluidic 
chip for screening 
drug compatibility, which comprises a fluid flow passage layer, a microvalve 
control channel layer and a glass substrate layer, wherein the fluid flow passage layer and the microvalve 
control channel layer are thermally bonded through PDMS to form a fluid flow passage unit, the fluid flow passage unit and the glass substrate layer form a whole through 
plasma bonding, the fluid flow passage unit comprises two functional areas, namely, a 
medicine mixing generation area and an array 
cell culture area. The invention further provides a preparation method and an application of the integrated micro-fluidic 
chip for screening 
drug compatibility; an SU-8 negative 
photoresist technology, an AZ-XT50 positive 
photoresist technology, a multi-layer PDMS 
thermal bonding technology and a 
plasma bonding technology are adopted, and the micro-fluidic 
chip integrating functions of automatic 
medicine mixing and generating, 
cell culture, excitation, parallel analysis on compatibility results of different drugs, real-time observation and result detection is prepared, multiple experiment parameters can be obtained through once operation, and the integrated micro-fluidic chip is applied to the research on the high-flux 
medicine compatibility relationship.