Integrated micro-fluidic chip for screening drug compatibility, as well as preparation method and application of integrated micro-fluidic chip

Abstract

The invention relates to an integrated micro-fluidic chip for screening drug compatibility, which comprises a fluid flow passage layer, a microvalve control channel layer and a glass substrate layer, wherein the fluid flow passage layer and the microvalve control channel layer are thermally bonded through PDMS to form a fluid flow passage unit, the fluid flow passage unit and the glass substrate layer form a whole through plasma bonding, the fluid flow passage unit comprises two functional areas, namely, a medicine mixing generation area and an array cell culture area. The invention further provides a preparation method and an application of the integrated micro-fluidic chip for screening drug compatibility; an SU-8 negative photoresist technology, an AZ-XT50 positive photoresist technology, a multi-layer PDMS thermal bonding technology and a plasma bonding technology are adopted, and the micro-fluidic chip integrating functions of automatic medicine mixing and generating, cell culture, excitation, parallel analysis on compatibility results of different drugs, real-time observation and result detection is prepared, multiple experiment parameters can be obtained through once operation, and the integrated micro-fluidic chip is applied to the research on the high-flux medicine compatibility relationship.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to an integrated microfluidic chip for drug compatibility screening, a preparation method and an application. Background technique [0002] Facing many challenges in the development of science and technology in the 21st century, analytical instruments and analytical science are also undergoing a revolutionary and important turning period. One of the increasingly obvious development trends is the miniaturization, integration and portability of chemical analysis equipment . Therefore, in order to meet the needs of the development of the times, the concept of Micro Total Analytical System (μTAS) was born. This kind of chip is used as the operating platform, based on analytical chemistry, based on micro-electromechanical processing technology, and micro-channel network Microfluidics (Microfluidics), also known as Lab-on-a-Chip (LOC), a multidisciplinary interdisciplinary tec...

AI Technical Summary

Problems solved by technology

[0006] At present, there are not many studies on microfluidic chips that integrate microvalves on a large scale and perform drug screening by manipulating microvalves, and this technology that can fully reflect the advantages of integration and flexibility is microfluidic chips. It is a very hot development trend in the field of high-throughput drug screening research in cells. However, due to the complexity of its processing, cumbersome operation and cross-integration of multidisciplinary knowledge, many researchers have little interest in this kind of integration. Drug screening chips with valves are prohibitive, resulting in the fact that drug screening microfluidic chips integrated with microvalves have not been able to be used in large-scale cell-level drug screening.

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