407 results about "Cultured Cell Line" patented technology

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.

This invention discloses a hepatocyte model with silencing or overexpression of LCMT1 and its construction method, comprising: passage and seeding cells in logarithmic growth phase into well plates; diluting plasmid siRNA1 or siRNA2 or an overexpression plasmid to an appropriate concentration, and then mixing it with transfection reagent dilution to obtain a transfection mixture; washing the cells in each well of the plate and adding another culture medium, then gently dripping the transfection mixture into the well plate, shaking well, and culturing in a cell culture incubator for a period of time; replacing the culture medium with complete culture medium without antibiotics to obtain the constructed hepatocyte model with silencing or overexpression of LCMT1. This invention, by constructing a hepatocyte model with silencing or overexpression of LCMT1 in the liver, allows for validation experiments to be conducted to study the development mechanism of liver fibrosis. It is suitable for widespread application in various laboratories and provides an experimental model basis for research on liver fibrosis and drug trials.

The application discloses a single-cell biopsy and dynamic transcriptome tracking system and method, and belongs to the technical field of single-cell sequencing. The system comprises the following steps: culturing single cells to be detected in a cell culture dish; puncturing the single cells by using an amino-modified quartz nanocapillary to extract trace cytoplasm samples; recovering the single cells in a culture environment; applying specific stimulation to the recovered single cells; at one or more time points after the stimulation is applied, puncturing the same single cells again by using the quartz nanocapillary to extract trace cytoplasm samples, and recovering the single cells after each extraction; and respectively performing reverse transcription, amplification, library construction and sequencing on the cytoplasm samples extracted at different time points to obtain dynamic transcriptome data of the single cells at different time points. The application can perform low-damage multiple longitudinal biopsies on the same single living cell, and can construct a dynamic transcriptome atlas of the single cell on a time axis in combination with downstream sequencing.

The utility model discloses a cell culture bottle inner environment parameter visual monitoring patch assembly, including operation platform and moving plate, the utility model discloses a moving plate is set up in the operation platform lateral wall, and one lateral wall of moving plate is provided with monitoring device, and the other lateral wall is installed with the monitoring probe that is connected with monitoring device, and the lateral wall of moving plate is installed with the extension spring, and the other end of extension spring is connected with operation platform, when using, the moving plate is pulled away from operation platform, and the cell culture bottle that is attached with the patch is placed in the operation platform top, and the extension spring pulls the moving plate and moves to the operation platform direction, and the monitoring probe is extruded in the cell culture bottle outer wall, and the cell culture bottle of different size can be monitored conveniently.

The invention provides methods for manufacturing pluripotent cells. In particular, the invention provides improved methods for manufacturing pluripotent cells with ground state pluripotency. In various embodiments, the invention contemplates, in part, use of a composition comprising: (a) a Wnt pathway agonist; (b) a MEK inhibitor; and (c) a ROCK inhibitor. In certain embodiments, the composition further comprises bFGF or LIF.

1. The name of the design product: cell culture device (with main machine). 2. The use of the design product: the design product is used for cell culture. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application provides a microfluidic chip and a single cell culture, cell group screening and derivation method. The chip comprises a substrate layer, a flow channel layer, an inlet flow channel, an outlet flow channel, a plurality of common flow channels and a plurality of functional units. The flow channel layer is located below the substrate layer, and the material of the flow channel layer comprises at least one of silicon dioxide, spin-on glass, non-photosensitive epoxy resin and non-photosensitive polyimide. The two ends of the common flow channel are respectively communicated with the inlet flow channel and the outlet flow channel. The functional unit comprises a single cell derivation inlet, a cell culture and screening cavity, a cell derivation cavity, a cell derivation outlet and a driving element. The driving element is used for providing power for liquid to introduce the single cell entering the common flow channel into the cell culture and screening cavity, and derive the target cell group cultured and screened from the cell derivation outlet. The application can complete the single cell culture, cell group identification and screening and target cell group derivation on the chip, is simple in experimental operation, reduces the risk of cross contamination and avoids the fluorescence interference of the flow channel layer itself.

The invention provides an aerosol-type face pack cosmetic that, when dispensed, forms a flexible, easy-to-use, and low-irritation resin foam that easily adheres to the face, such as around the eyes and nose. It also allows the fermented liquid contained in the resin foam to easily seep out, making it easy to apply the fermented liquid to the face and less likely to cause tightness. [Solution] An aerosol pack cosmetic comprising a beauty serum and dimethyl ether, wherein the beauty serum comprises at least one fermentation solution selected from Saccharomyces / Anzange ferment filtrate, Saccharomyces / Human (fibroblast / mononuclear cell) induced pluripotent cell culture conditioned medium ferment filtrate, Galactomyces / Eryngium multiflorum callus culture ferment filtrate, and a resin.

This invention provides a constant-flow nutrient solution replenishment device for cell culture, relating to the field of cell culture technology. It includes a base, an internally connected storage tank, a top-mounted lid, a fixedly connected inlet cylinder at the bottom of the storage tank, and an operating box fixedly connected to the top of the inlet cylinder. A constant-flow component is mounted on the outer surface of the operating box, comprising a drive unit and an output rod fixedly connected to the output end of the drive unit. This invention features a base supporting the storage tank, facilitating the flow of nutrient solution from the bottom inlet hole into the inlet cylinder. The inlet cylinder and guide plate are designed to introduce the nutrient solution into the culture chamber. The dust cover prevents dust from falling into the culture chamber during the nutrient solution's flow. The operating box serves the same purpose as the dust cover and also houses a constant-flow component for controlling the constant flow of the nutrient solution.

Various aspects of the present disclosure relate to a fermentation culture medium supplement that comprises a soluble fraction derived from a leguminous plant. Provided herein, in some aspects, are compositions comprising the soluble fraction derived from a leguminous plant described herein, and methods of making the soluble fraction derived from a leguminous plant.

A cell culture device according to the present disclosure comprises: a culture chamber for culturing suspension cells; a flow path having a culture medium supply port and a culture medium discharge port, and provided adjacent to part or all of the outer side of at least one surface of the culture chamber; an oxygen permeable membrane provided to at least part of the flow path and / or the culture chamber; and a cell seeding port provided to at least part of the culture chamber.

Disclosed herein is a cell culture device (1) for culturing cells and / or organoids, the cell culture device comprising a cell containment unit (2) comprising at least one cell culture chamber (3) configured to contain a liquid cell culture medium (9) and a cell structure, wherein the cell culture chamber (3) comprises: a gas vent opening (4) configured to vent the cell culture chamber; a pressure chamber (5) comprising a pressure chamber inlet (6); a channel (7) connecting the at least one cell culture chamber (3) and the pressure chamber (5); the cell culture device further comprising a first pressure generator (8), in particular a pump, connected to the pressure chamber inlet and configured to temporarily pressurize the pressure chamber with a pressure pulse; wherein the at least one cell culture chamber (3), the pressure chamber (5) and the channel (7) are configured such that the pressure pulse from the first pressure generator (8) propagates from the pressure chamber (5) through the channel (7) to the at least one cell culture chamber (3) and in particular into the liquid cell culture medium (9).

1. The name of the design product: cell culture device. 2. The use of the design product: the design product is used for cell culture. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belongs to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The application discloses a kind of mesenchymal stem cell ball fractional dissociation digestive juice, its preparation method and application, it is related to cell preparation and three-dimensional cell culture technical field, specifically at to provide C liquid, D liquid and E liquid three kinds of gradient concentration working solution.When dissociating, the mesenchymal stem cell ball to be dissociated is determined its average particle size;According to the average particle size, select C liquid, D liquid or E liquid as digestive juice as described above;The selected digestive juice is mixed with the mesenchymal stem cell ball, and digestion reaction is carried out under oscillation condition, and single cell suspension is obtained.The application establishes the hierarchical matching rule of mesenchymal stem cell ball particle size and specific digestive juice concentration, and optimizes oscillation digestion condition, so as to maintain high activity rate and complete phenotype characteristics while efficiently dissociating, realizes the standardization of process and the controllable of dissociation quality, provides stable and reliable solution for the large-scale preparation and quality control of mesenchymal stem cell ball.

Devices for filtering liquids are provided herein. More particularly, the devices may be devices for separating biological cells from microcarriers the cells have been grown with, as well as other debris and materials, that are found in the cell culturing liquid. The devices may also be foldable or collapsible.

A method of coating a cell culture substrate in situ in a bioreactor is provided that includes providing a bioreactor vessel having a cell culture chamber with an inlet for flowing fluid into the cell culture chamber and an outlet for flowing fluid out of the cell culture chamber, and a cell substrate disposed in the cell culture chamber for culturing cells. The method includes providing a coating solution for coating the cell substrate; inputting the coating solution into the cell culture chamber such that the coating solution contacts the cell substrate; and removing an excess of the coating solution from the cell culture chamber. After removing the coating solution, a coated cell substrate remains in the cell culture chamber.

This invention discloses a method for culturing cherry cells and cherry cell exosomes. The culturing method includes the following steps: S1. Obtaining a cherry cell line; S2. Preparing a culture medium by adding salicylic acid and methyl jasmonate; S3. Culturing the cherry cell line obtained in step S1 under the following conditions: culturing at 20–30°C with UV-B illumination during the culture process. This invention improves the cherry cell culture method, increases production efficiency, and enhances the content of active ingredients, especially flavonoids and polyphenols, in the cherry cell culture. The cherry cell culture obtained by this invention, especially the exosomes extracted from it, has broad application potential in the cosmetics field.

The technical problem this invention aims to solve is to provide a device for aseptic bagging of cell suspension in culture bags. This device effectively avoids cell suspension exposure and reduces the risk of contamination. The device includes a reagent bottle and a feeding bottle cap. A dispensing tube is installed on the feeding bottle cap. The lower end of the dispensing tube is connected to a sampling tube, a sampling head, and a shunt tube via a first Y-shaped connecting fitting. The lower end of the shunt tube is connected to a first shunt branch and a second shunt branch via a second Y-shaped connecting fitting. A third clamp structure is installed on the first shunt branch, and a fourth clamp structure is installed on the second shunt branch. A cell culture bag or a venting tube is connected to the lower end of the first and second shunt branch via a connector. An air filter is installed at the lower end of the venting tube. An air inlet component is also installed on the feeding bottle cap. This device ensures that the cell suspension is not exposed during the entire operation, effectively reducing the risk of contamination during the bagging process.

The present disclosure relates to cell culture vessels and methods of using the same that are capable of providing a laminar flow of cell culture media to cells in the cell culture vessels. The cell culture vessels include a plurality of wells in which cells reside as media moves across the top thereof. The cell culture system is further enclosed to maintain sterility. The media can be constantly renewed within the cell culture vessel, can be recirculated within the cell culture vessel, or a combination of both. Circulation of media around the cells residing therein can be particularly useful for 3D tissue culture, such as for producing spheroids or organoids.

The present invention relates to a hydroalcoholic extract derived from plant cell cultures belonging to the species Hyssopus officinalis and to a method for the preparation thereof. The invention further relates to a cosmetic composition or formulation comprising said extract, and to the use of the extract and / or the cosmetic composition or formulation containing said extract for skin care, in particular for strengthening the skin barrier. The cosmetic composition or formulation containing the extract according to the invention can be prepared in any form suitable for topical application, such as cream, gel, lotion, ointment, emulsion for use in lipsticks, foundations and other make-up products, or in anhydrous form.

In the field of cell-based assays, i.e. cellular assays, there is a need for improved cell culture retaining inserts and cell culture retaining insert assemblies. A cell culture retaining insert (10; 70; 90; 100), for retaining a three-dimensional biological sample in a cell culture vessel (102; 132), comprises an insert body (12; 104) that has a positioning formation (16) to in-use locate the cell culture retaining insert (10; 70; 90; 100) relative to a cell culture vessel (102; 132). The insert body (12; 104) also has a retention element receiving formation (20). The cell culture retaining insert (10; 70; 90; 100) additionally includes a retention element (30; 60; 72; 92; 114) which is movably coupled with the receiving formation (20) of the insert body (12;104), whereby a distal retention end (32) of the retention element (30; 60; 72; 92; 114) is freely movable away from and towards the insert body (12; 104), and whereby in-use the weight of the retention element (30; 60; 72; 92; 114) less a buoyant force equal to the weight of any fluid the retention element (30; 60; 72; 92; 114) displaces gives rise to the retention end (32) exerting a retention force on one or more said biological samples.

The process provided in this invention transfects primary cells with gene editing reagents using a high-volume gas-permeable cell culture device and a flow-through electroporation device under conditions that improve gene editing performance, cell yield, and drug (DP) quality characteristics for cell therapy applications. As demonstrated in the examples, primary cells edited according to the process provided herein achieved improved double-strand break (DSB) formation rates, increased frequency of homology-directed repair (HR) and non-homologous end joining (NHEJ) combinations, increased frequency of bi-allelic and mono-allelic HR events, improved cell viability, proliferative capacity, and cell fitness after gene editing, and reduced manufacturing time.

This utility model belongs to the field of cell culture technology, specifically disclosing a cell culture replenishment device, including a laminar flow hood and a tank. The tank is fixed to the top of the laminar flow hood, and a sleeve is movably connected to the top of the tank. A protective sleeve is fixed to the left side of the sleeve. A second ultraviolet lamp is installed in the upper left corner of the tank. A frame is fixed to the center of the top of the tank. This cell culture replenishment device stores sterilized culture medium in the tank. A motor rotates a stirring rod to guide the culture medium through a liquid pump and inject it into a culture dish on the laminar flow hood. After use, the inner wall is cleaned first, then the door is closed and the first ultraviolet lamp is turned on to disinfect the laminar flow hood. The tank is then sterilized by the second ultraviolet lamp. After the sterilization is complete, a dial is turned so that the protective sleeve fits onto the surface of the second ultraviolet lamp for protection, preventing the replenishment device from being contaminated with bacteria and solving the problem of lacking an internal cleaning mechanism.

The application provides a high-activity iPSC exosome and a preparation method and application thereof, relates to the technical field of biological medicine, and the preparation method comprises the following steps: obtaining induced pluripotent stem cells; performing flow cytometry sorting to obtain an iPSC subpopulation; performing suspension expansion culture on the iPSC subpopulation to collect a cell culture solution; sequentially performing clarification filtration, ultrafiltration concentration and CD9 antibody affinity chromatography purification, and then eluting and collecting an eluent containing exosomes. The induced pluripotent stem cells overcome donor dependence and batch difference. The specific screening of a cell subpopulation that is positive for CD105, CD73 and CD90 and maintains pluripotency eliminates heterogeneity and guarantees the high activity and uniform efficacy of the exosomes. The use of microcarriers for serum-free suspension culture breaks through the yield bottleneck and realizes large-scale production. Finally, the high-purity and high-activity exosomes are efficiently extracted through ultrafiltration and CD9 antibody affinity chromatography purification.

The application discloses a preparation method and application of large-size human liver organoids, and belongs to the technical field of cell culture. In the application, 2D culture mature human primary liver parenchymal cells with different cell amounts are resuspended by using a hepatocyte maturation medium HIM, and are added into a U-shaped bottom 96-hole plate for low-oxygen static culture. After the cells are aggregated, the cells are cultured in normal oxygen and are shaken, so that large-size human liver organoids with different cell contents are prepared. The method does not need biological materials, and can induce human primary liver parenchymal cells to self-assemble and construct functional liver organoids with high uniformity in scale through specific culture conditions. The method solves the problems of large batch difference, high heterogeneity and dedifferentiation in the prior art, and can be widely applied to drug hepatotoxicity testing, in-vitro modeling of liver diseases and development of a bioartificial liver system.

The utility model discloses an easy stacking type cell culture device belongs to cell culture device technical field, including base, the top of base is provided with cell culture device body, and the middle fixed connection of cell culture device body one side is provided with observation window, and cell culture device body one side one end fixed connection has control panel, and cell culture device body one side the other end fixed connection has nameplate, and the convenient fixed assembly connection between base and cell culture device body, and the convenient fixed assembly connection between the adjacent cell culture device body, and the both sides of base are provided with positioning assembly, the utility model sets up convenient fixed assembly, and the cell culture device main part is stacked, can be fixed conveniently, thereby improves the stability of cell culture device body stacking, and the utility model discloses through positioning assembly, makes the device to be placed stably on the desktop of experiment table.

A microcavity bioreactor is provided that allows cell growth and cell differentiation in the same vessel, such that higher order three-dimensional cell cultures such as organoids can be generated in a singular vessel. The microcavity bioreactor may be part of a microcavity bioreactor system that allows for perfusion based cell culture and perfusion based cell harvesting. The microcavity bioreactor includes at least one, and preferably at least two, fluid distributor structures in the housing vessel of the microcavity bioreactor.