3809 results about "Cultured Cell Line" patented technology

The invention relates to the technical field of cell culture data processing, in particular to a cell culture information data management method and system. The method comprises the following steps: performing culture environment multi-parameter high-frequency real-time monitoring on a cell culture environment box to obtain multi-dimensional cell culture environment time sequence parameters; performing culture environment key disturbance parameter screening on the multi-dimensional cell culture environment time sequence parameters to generate environment key disturbance parameters; acquiring historical cell culture batch data; based on historical cell culture batch data, carrying out multi-parameter coordinated regulation and control on the environment key disturbance parameters to obtain a cell culture environment regulation and control strategy; regulating and controlling the cell culture process according to the cell culture environment regulation and control strategy, and performing cell culture knowledge graph construction on historical cell culture batch data to obtain a cell culture management knowledge graph. According to the invention, intelligent management of cell culture data is realized through construction of the cell culture knowledge graph.

This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

The invention discloses an abnormal monitoring method and system for immune cell culture. The method comprises the following steps: collecting cell physical state parameters, culture environment parameters and metabolic biochemical indexes in a culture system in real time; performing motility rate threshold judgment and morphological analysis based on the cell physical state parameters to generate a first abnormal signal; performing dynamic trend analysis on the metabolism biochemical indexes to generate a second abnormal signal; performing grade association on the culture environment parameters, the first abnormal signal and the second abnormal signal, and outputting a monitoring abnormal grade; and performing grading response construction according to the monitoring abnormity grade to obtain an abnormity monitoring report. According to the method, the pollution diffusion risk and the functional cell failure misjudgment rate can be reduced.

The invention relates to the technical field of medical image analysis, in particular to a CAR-T cell culture monitoring system based on image recognition, which comprises a cell region division module, a cell morphology analysis module, a cell behavior dynamic analysis module and a cell population collaborative monitoring module. According to the method, through cell image gradient intensity analysis, refined cell edge detection and dynamic adjustment of a segmentation threshold value, high-precision extraction of cell boundaries is ensured, noise interference is avoided, cell shapes, textures and geometric features are refined, the identification degree of morphological features is improved, and tiny changes of cell morphologies are accurately captured; dynamic tracking and trend evaluation are carried out on cell behaviors based on adjacent frame images, tiny dynamic changes such as cell division, aggregation and migration are accurately reflected, excessive smoothness in the dynamic process is avoided, cell population behavior monitoring is combined with interaction and corresponding speed calculation, the population synergistic effect change trend is comprehensively evaluated, and the cell population behavior monitoring effect is improved. And finer cell culture environment optimization and clinical research support are provided.

The invention provides a cell culture medium aided design system and method based on a large oracle model, and belongs to the technical field of artificial intelligence. A plurality of candidate culture medium formula schemes meeting user requirements are generated by obtaining culture medium design parameters and requirements and designing structured cues and inputting the designed structured cues into a large language model; a substance matched with the structured cue word is retrieved by utilizing a retrieval enhancement technology, a replacement substance is recommended for a user to refer, the user selects and purchases a required experimental material according to own requirements, a candidate culture medium formula scheme is fed back and evaluated in an actual experiment, and the system performs iterative optimization based on the feedback and evaluation, so that the accuracy of the experiment is improved. And a more appropriate candidate culture medium formula scheme is provided. According to the cell culture medium aided design system and method, intelligence, scientization and individuation of culture medium formula scheme design are achieved, experiment cost is reduced, repeatability and stability of cell culture experiments are improved at the same time, and therefore wide application of culture medium design in the field of biological manufacturing is promoted.

Embodiments of the invention include additional methods and compositions for fabricating organoids. A unique approach has been developed for the rapid aggregation of defined number of cells using a micropillar-based template to limit the size of organoids, which are then more commonly referred to as spheroids. This procedure can be utilized to create in vitro cardiac tissue models (e.g., cardiac organoids) simply, rapidly, and inexpensively. In certain aspects an alginate-gelatin based hydrogel premixed with cardiomyocytes is used in combination with a patterned microfluidic chip / cell culture chamber having an array of micropillars to fabricate 3D cardiac organoids.

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

The invention discloses a preparation method and application of a cell culture liver based on a microcarrier. The method comprises the steps of obtaining liver seed cells, preparing the microcarrier, culturing cells in vitro based on the microcarrier, preparing the cell culture liver and the like. The preparation method of the cell culture liver based on the microcarrier is provided for the first time, and the preparation scheme that a cell-microcarrier compound serves as a structural unit and is assembled into the cell culture liver after being mixed with an edible material is adopted, so that on one hand, simulation of a real liver lobule structure can be achieved, and on the other hand, simulation of the liver lobule structure can be achieved; the whole structure relation of liver lobules and connective tissues in liver tissues can be simulated, full reduction of animal liver appearance and structural characteristics is achieved, edible materials used in the preparation process are low in price and good in biocompatibility, and the preparation method is suitable for large-scale industrial production. Liver seed cells can be subjected to efficient amplification and albumin synthesis through three-dimensional culture on a microcarrier prepared on the basis of edible materials, so that the cell culture liver is close to a real animal liver in the aspect of nutrition.

The invention provides cell culture monitoring equipment which comprises a cell culture box and a monitoring module, the cell culture box is used for installing a micro-fluidic chip and further comprises a first observation window, the first observation window is used for exposing an observation area of the micro-fluidic chip, and the monitoring module comprises an imaging assembly. The imaging assembly is used for collecting an image of the micro-fluidic chip from the first observation window. The cell culture monitoring equipment can realize real-time and online monitoring of the cell growth process.

The invention provides a mesenchymal stem cell culture monitoring system based on multiple parameters, and relates to the technical field of data processing, and the mesenchymal stem cell culture monitoring system comprises a first-level control instruction generation module which is used for generating a second-level control instruction when the metabolism-morphological abnormality association intensity in a combined fuzzy state matrix is judged to reach a preset association threshold value through a fuzzy rule base; when the abnormal state flag bit indicates that the isolated metabolism is abnormal, a second-level control instruction is generated; the driving unit is used for responding to the first-stage control instruction and cooperatively driving the buffer solution injection unit and the nutrition supplementing pump; responding to the secondary control instruction, and dynamically adjusting the opening degrees of a stirring rate controller, a temperature feedback loop and a gas proportional valve of the bioreactor. The control efficiency and the cell culture stability are improved.

The invention discloses a method for transdifferentiating tail fin cells of erythroculter ilishaeformis into fat and muscle cells and application, the method is based on 5-Azacytidine (azacytidine) to promote mesenchymal stem cells to be differentiated into muscle cells, and other small molecule compounds are added on the basis of adding the 5-Azacytidine to transdifferentiate the tail fin cells of erythroculter ilishaeformis into muscles. The method is realized through an in-vitro cell reprogramming technology, fish individuals do not need to be sacrificed, and the destructive influence of traditional muscle or fat sampling on fish bodies is avoided; a single-step induction system does not need multi-factor sequential treatment, a high-purity target cell population can be obtained only in 5-7 days at least, the standardization degree of the operation process is high, the stability among batches is excellent, specific cells can be continuously and stably produced, and a safe and reliable technical basis is provided for sustainable cell culture fish meat production.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention relates to a triple-hole paper-based three-dimensional cell culture chip as well as a preparation method and application thereof. The chip comprises a paraffin hydrophobic region and a paper-based hydrophilic region. The preparation method mainly comprises the following steps: 1) designing a mask plate; 2) cutting the mask plate by laser; and 3) preparing the triple-hole paper-based chip by hot pressing. The thickness of the chip is 100-200 [mu] m, the porous fiber structure of the paper-based material allows transportation of oxygen and waste, and a three-dimensional microenvironment for in-vivo cell growth can be simulated. Meanwhile, a transparent and liquid-tight supporting layer is fused on the lower layer of the paper-based chip, so that leakage of cells inoculated on the upper layer of the paper-based chip is avoided. The paper-based chip is embedded into a 6-pore plate, and bladder cancer cells (5637), mouse astrocytoma (U87) and human breast cancer cells (MCF-7) are inoculated in a paper-based hydrophilic region respectively. The cell compatibility of the paper-based chip and the growth behaviors of three different types of cells in three-dimensional paper fibers are investigated. It is proved that the chip can be used for researching three-dimensional construction and growth of human and mammalian cells, and the difference between three-dimensional culture and two-dimensional culture is revealed by detecting the proliferation capacity and survival rate of different cells.

The invention relates to a cell culture system (1) comprising: A bioreactor (100), comprising a vessel (110) and a recycling outlet (120), said recycling outlet (120) being positioned in the vessel (110) so as to be able to extract, from the vessel (110), a multiphase flow comprising a liquid phase and a gas phase; a separator (300) receiving the multiphase flow, said separator (300) being configured to accommodate and maintain a multiphasic system, comprising both the liquid phase and the gas phase; and a recycled media transfer line (220) adapted for routing of at least a portion of the liquid phase from the separator (300) to the bioreactor (100). The invention also relates to a cell culture method (1000) using a cell culture system (1) of the invention and cell biomass obtained thereof.

The invention discloses a filling and repairing agent for promoting angiogenesis and a preparation method thereof, the filling and repairing agent is composed of a vascular endothelial cell supernatant, acellular matrix particles, a suspending aid and a protective agent, the vascular endothelial cell supernatant is prepared by inducing vascular endothelial cells into a tube under a low-oxygen three-dimensional condition, collecting a cell culture solution, centrifuging and concentrating; the acellular matrix particles are obtained by acellular tissue matrix, shearing, freeze-drying, liquid nitrogen grinding and collecting; according to the filling repairing agent for promoting angiogenesis, the vascular endothelial cell supernate and the acellular matrix particles are combined, the filling repairing agent contains high-concentration angiogenesis-related factors and a stable three-dimensional bionic scaffold structure, function complementation is achieved through the synergistic effect of the bioactive factors and the bionic scaffold, and the filling repairing agent for promoting angiogenesis is obtained. Therefore, the filling repair agent which can promote angiogenesis, construct a physical support framework and promote tissue repair and regeneration capacity is innovatively obtained.

The invention discloses a cord blood-derived regulatory T cell amplification culture medium and a use method thereof, and relates to the field of animal cell culture and immune cell therapy, the culture medium is a serum-free culture medium, the culture medium is composed of a basic culture medium, a serum substitute combination, a combination of four cell factors, an immunosuppressor, an apparent stabilizer and an antioxidant, and all the components play a role synergistically; according to the use method, cell amplification is realized through a collaborative process of staged environmental regulation and targeted activation. According to the method, the problems of exogenous pollution and batch difference caused by dependence of a serum-containing system on cord blood-derived regulatory T cell amplification in the prior art are solved, the defect that a serum-free scheme is difficult to consider the amplification efficiency and the function stability at the same time is overcome, the cell amplification quality and the clinical application safety are guaranteed, and the method is suitable for large-scale clinical transformation requirements.

The utility model relates to the technical field of cell cultivators, in particular to a pump-free perfusion micro-fluidic chip for multi-cell three-dimensional co-culture, which comprises a liquid storage layer, a liquid inlet and outlet layer, an upper channel layer, a lower channel layer and a packaging layer, realizes a one-way circulation perfusion dynamic co-culture system without a pumping system, reduces the occupied space of equipment, and is convenient to operate. Operation is simple, culture medium consumption is reduced, and cost is reduced; the separation of free NK cells and tumor infiltration NK cells is realized, and conditions are provided for researching molecular biological characteristics of different types of NK cells. The migration and interaction of the tumor spheres and the NK cells are observed in real time at the same time, so that the problem that a microfluid pump integrated by an external system is needed for separating free immune cells and tumor infiltration immune cells by existing equipment is solved.

The invention discloses a force-position control bottle cap screwing device and method supporting the digital twinning technology. The force-position control bottle cap screwing device comprises a screwing clamping jaw mechanism used for screwing a bottle cap; the Z-axis executing mechanism and the X-axis executing mechanism are used for adjusting the position of the screwing clamping jaw mechanism, the screwing clamping jaw mechanism is fixed to the output end of the Z-axis executing mechanism, and the Z-axis executing mechanism is fixed to the output end of the X-axis executing mechanism; the force sensor and the position sensor are used for detecting the stress and position information of the screwing clamping jaw mechanism; and the control unit is used for receiving signals of the force sensor and the position sensor and regulating and controlling the stress and the position of the screwing clamping jaw mechanism according to the received signals. The requirement for opening and closing the cell culture fluid bottle cap to take fluid in the intelligent and automatic production process of the organoids can be met, and technical and theoretical possibilities are provided for successful research and development of the intelligent and automatic production line for culturing and observing the organoids.

The invention relates to the technical field of medical cell culture, and discloses a medical experiment cell culture device which comprises a culture box, an electric push rod is fixedly connected to the lower surface of the culture box, a supporting plate is fixedly connected to the output end of the electric push rod, and a first connecting column is fixedly connected to the outer wall of the supporting plate; a first partition plate is fixedly connected to the inner wall of the incubator, a sliding groove is formed in the first partition plate, the outer wall of the first connecting column is slidably connected to the inner wall of the sliding groove, and a movable frame is rotatably connected to the outer wall of the first connecting column. The electric push rod is started to drive the supporting plate to move up and down, and then the connecting column drives the moving frame to move synchronously. The movable frame drives the transmission plate to move upwards, the frame plates at the two ends are pulled or pushed to move relatively through the fixing columns, then the closing plate is driven to be opened and closed, meanwhile, the placing frame can be moved into a test box or stored into a culture box, operation convenience and efficiency are improved, and the risk that the culture environment is polluted by manual taking is avoided.

The invention discloses a culture medium and application thereof, and a method for in-vitro separation culture and adipogenic differentiation of precursor adipocytes in goat muscle, and relates to the technical field of cell culture, the culture medium comprises the following components: 8-12 [mu] g / mL of insulin, 8-12% by volume of fetal calf serum, 95-105 U / mL of penicillin, 95-105 [mu] g / mL of streptomycin and the like. On the basis of the prior art, a proper amount of liquiritin and pilocarpine are mainly added into a culture medium, and synergistic cooperation of the liquiritin and pilocarpine is found, so that the adipogenic differentiation capacity of cells such as mesenchymal stem cells and precursor adipocytes is remarkably improved, and the application range is wide. On the basis, the invention also provides a complete set of perfect in-vitro isolated culture and adipogenic differentiation method of the goat intramuscular precursor adipocytes, and a method and a way are provided for a series of researches developed through the goat primary intramuscular precursor adipocytes in the future.

The invention discloses a method for culturing CIK (Cytokine-Induced Killer) cells and application thereof. The method comprises the following steps: recovering cord blood, mixing and centrifuging the recovered cord blood and a buffer solution, re-suspending and precipitating, centrifuging again to obtain cord blood mononuclear cells, inoculating the cord blood mononuclear cells into an activation culture medium, and culturing to obtain the CIK cells, the buffer solution contains L-proline, human recombinant DNA enzyme and angelica polysaccharide. According to the method, the mononuclear cells can be separated from the umbilical cord blood preserved by liquid nitrogen, the utilization rate of the umbilical cord blood which can only be discarded due to insufficient cells is increased, the traditional lymphocyte separation process is optimized, the recovery rate of the mononuclear cells is increased, and the cost is reduced. The method provided by the invention can be used for culturing dozens to ten billions of CIK cells from a small amount of cryopreserved cord blood, and has huge advantages in the field of CIK cell culture and application.

The present invention refers to a method for coating a collagen like protein onto a surface of a polymer-scaffold comprising or consisting the following steps: a) plasma-treating the surface of the polymer-scaffold, b) providing a collagen like protein in a solvent, to obtain a collagen like protein-containing solution, c) bringing the plasma treated polymer-scaffold and the collagen like protein containing solution into contact followed by an optionally rinsing, d) removing the solvent and thereby obtaining a collagen like protein coated polymer-scaffold. Furthermore, the present invention refers to an article obtained by the method of the present invention. Moreover, the present invention refers to the use of the article according to the invention for wound healing and bone repair as well as for flask-coating and plate-coating in cell culture.

The utility model relates to the technical field of cell culture, and discloses a chamber structure adaptive to Faraday wave assembly, which comprises a pore plate, N mounting grooves are formed in the top surface of the pore plate, and N is greater than or equal to 1; the N container bodies are arranged on the pore plate in a rectangular array mode or a central symmetry mode and correspondingly arranged in the N mounting grooves, a plurality of culture grooves used for cell assembly are sequentially formed in the container bodies in the longitudinal direction from the top face to the bottom face, and the widths of the culture grooves are gradually decreased. According to the device, internal cells or cell microspheres can be gathered into a sound potential well of a standing wave sound field under the action of acoustic pressure, gravity and buoyancy under the action of the sound field and are tightly arranged according to an expected pattern, and a culture solution is longitudinally stacked in a plurality of culture tanks of which the widths are gradually reduced; longitudinal shape limitation on the tissue structure is realized, and complex three-dimensional longitudinal arrangement is constructed through mutual combination of the two. Therefore, the requirement of constructing any multi-scale and complex cell structure is met. And forming a multi-layer structure.

The utility model discloses a micro-fluidic chip for liver multi-cell co-culture, which belongs to the technical field of biomedical engineering and comprises a perfusion layer and a culture layer, and the perfusion layer is provided with a first culture solution inlet and a culture medium outlet; the culture layer is provided with a micro-perfusion liver chip; bonding and forming the perfusion layer and the culture layer; two groups of micro-perfusion liver chips are arranged, and each group of micro-perfusion liver chips comprises a set of micro-perfusion network system; the micro-perfusion network system comprises a micro-perfusion channel, a multifunctional cell co-culture area and a liquid storage tank, the micro-perfusion channel, the multifunctional cell co-culture area and the liquid storage tank are communicated, and the micro-perfusion network system is communicated with the first culture solution inlet and the culture medium outlet; the multifunctional cell co-culture area comprises four cell culture areas. The problems that in the prior art, the liver structure is complex, and experimental observation and operation are difficult are solved. The device has the advantages that the in-vivo environment and interaction of liver organ diseases are fully simulated, and observation is convenient.

The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.

The invention discloses a cell culture box convenient for taking and placing cell culture dishes, and relates to the technical field of cell culture, the cell culture box comprises a box body, the inner wall of the box body is fixedly connected with a plurality of placing plates, and the side walls of the top ends of the plurality of placing plates are provided with a plurality of first grooves. The inclination angle of the second clamping plate is the same as that of the outer wall of the corresponding cover plate, so that the cover plate is screwed above the cell culture dish by utilizing the second clamping plate, the primary installation between the cell culture dish and the cover plate is completed, and then a buckle on the outer side of the cover plate can be pressed into a clamping groove in the outer wall of the cell culture dish through a clamping block; when the cell culture dish needs to be taken out, a buckle is pulled out of a clamping groove in the outer wall of the cell culture dish by utilizing a pulling plate, and then the cover plate is taken down from the cell culture dish by utilizing a second clamping plate, so that the culture efficiency and reliability during cell culture are greatly improved.

The present invention relates to an integrated system for automating cell culture processes. The system combines artificial intelligence (AI) with a robotic apparatus to execute tasks typically performed manually in cell culture laboratories. The AI module employs machine learning algorithms trained on extensive datasets, enabling it to make informed decisions regarding cell culture conditions and protocols for a variety of cell types. An accompanying robotic system performs liquid handling tasks such as media changes and cell passaging with precision. The system is compatible with multiple types of cell culture vessels, facilitating bulk processing. Additionally, an enclosed sterile environment is maintained to prevent contamination. A user interface allows for the customization of protocols and remote monitoring, enhancing operational efficiency. This invention streamlines cell culture workflows, reduces manual labor, and increases the reproducibility and scalability of cell culture.

The present invention refers to a method for coating a collagen like protein onto a surface of a polymer-scaffold comprising or consisting the following steps: a) plasma-treating the surface of the polymer-scaffold, b) providing a collagen like protein in a solvent, to obtain a collagen like protein-containing solution, c) bringing the plasma treated polymer-scaffold and the collagen like protein containing solution into contact followed by an optionally rinsing, d) removing the solvent and thereby obtaining a collagen like protein coated polymer-scaffold. Furthermore, the present invention refers to an article obtained by the method of the present invention. Moreover, the present invention refers to the use of the article according to the invention for wound healing and bone repair as well as for flask-coating and plate-coating in cell culture.