5463 results about "Cultured Cell Line" patented technology

This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

The invention relates to the technical field of cell culture data processing, in particular to a cell culture information data management method and system. The method comprises the following steps: performing culture environment multi-parameter high-frequency real-time monitoring on a cell culture environment box to obtain multi-dimensional cell culture environment time sequence parameters; performing culture environment key disturbance parameter screening on the multi-dimensional cell culture environment time sequence parameters to generate environment key disturbance parameters; acquiring historical cell culture batch data; based on historical cell culture batch data, carrying out multi-parameter coordinated regulation and control on the environment key disturbance parameters to obtain a cell culture environment regulation and control strategy; regulating and controlling the cell culture process according to the cell culture environment regulation and control strategy, and performing cell culture knowledge graph construction on historical cell culture batch data to obtain a cell culture management knowledge graph. According to the invention, intelligent management of cell culture data is realized through construction of the cell culture knowledge graph.

This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

The invention discloses an abnormal monitoring method and system for immune cell culture. The method comprises the following steps: collecting cell physical state parameters, culture environment parameters and metabolic biochemical indexes in a culture system in real time; performing motility rate threshold judgment and morphological analysis based on the cell physical state parameters to generate a first abnormal signal; performing dynamic trend analysis on the metabolism biochemical indexes to generate a second abnormal signal; performing grade association on the culture environment parameters, the first abnormal signal and the second abnormal signal, and outputting a monitoring abnormal grade; and performing grading response construction according to the monitoring abnormity grade to obtain an abnormity monitoring report. According to the method, the pollution diffusion risk and the functional cell failure misjudgment rate can be reduced.

The invention relates to the technical field of medical image analysis, in particular to a CAR-T cell culture monitoring system based on image recognition, which comprises a cell region division module, a cell morphology analysis module, a cell behavior dynamic analysis module and a cell population collaborative monitoring module. According to the method, through cell image gradient intensity analysis, refined cell edge detection and dynamic adjustment of a segmentation threshold value, high-precision extraction of cell boundaries is ensured, noise interference is avoided, cell shapes, textures and geometric features are refined, the identification degree of morphological features is improved, and tiny changes of cell morphologies are accurately captured; dynamic tracking and trend evaluation are carried out on cell behaviors based on adjacent frame images, tiny dynamic changes such as cell division, aggregation and migration are accurately reflected, excessive smoothness in the dynamic process is avoided, cell population behavior monitoring is combined with interaction and corresponding speed calculation, the population synergistic effect change trend is comprehensively evaluated, and the cell population behavior monitoring effect is improved. And finer cell culture environment optimization and clinical research support are provided.

The invention discloses an organ chip capable of simulating a physiological microenvironment in a joint and application of the organ chip. The organ chip comprises a cell culture layer 1, an elastic film 2, an air pressure control layer 3, a cell culture channel 4, a cell culture fluid perfusion channel 5, a cell suspension inlet 6, a cell suspension outlet 7, a culture fluid perfusion inlet 8, a culture fluid perfusion outlet 9, a through hole 10, a through hole 11, a first air pressure groove 12, a second air pressure groove 13, a pneumatic chamber 14, a pneumatic chamber 15, a cell bearing platform 16 and a matrix 17. The organ chip can simulate physical factors such as dynamic compression and relative sliding in joint movement in vitro, and the physical factors are combined with chemical stimulation factors.

The invention provides a cell culture medium aided design system and method based on a large oracle model, and belongs to the technical field of artificial intelligence. A plurality of candidate culture medium formula schemes meeting user requirements are generated by obtaining culture medium design parameters and requirements and designing structured cues and inputting the designed structured cues into a large language model; a substance matched with the structured cue word is retrieved by utilizing a retrieval enhancement technology, a replacement substance is recommended for a user to refer, the user selects and purchases a required experimental material according to own requirements, a candidate culture medium formula scheme is fed back and evaluated in an actual experiment, and the system performs iterative optimization based on the feedback and evaluation, so that the accuracy of the experiment is improved. And a more appropriate candidate culture medium formula scheme is provided. According to the cell culture medium aided design system and method, intelligence, scientization and individuation of culture medium formula scheme design are achieved, experiment cost is reduced, repeatability and stability of cell culture experiments are improved at the same time, and therefore wide application of culture medium design in the field of biological manufacturing is promoted.

The invention discloses a porcine muscle stem cell line capable of being cultured in vitro for a long time and application of the porcine muscle stem cell line, and belongs to the technical field of stem cells and cell culture meat. The porcine muscle stem cell line is prepared by the following steps: performing CDKN2A gene knockout on porcine muscle stem cells by using a CRISPR / Cas9 gene editing technology, performing monoclonal screening and genotype identification to obtain a cell strain with successful CDKN2A knockout, and then performing carrier-free and serum-free suspension culture domestication on the cell strain. And a carrier-free and serum-free porcine muscle stem cell line capable of being subjected to in-vitro long-term suspension culture is obtained. Verification shows that the CDKN2A knockout porcine muscle stem cell line subjected to carrier-free and serum-free suspension culture domestication not only can be subjected to carrier-free and serum-free suspension culture in a shake flask for a long time, but also can maintain a good differentiation characteristic. The porcine muscle stem cell line which can be cultured on a large scale, is derived from edible animals and can be cultured in vitro for a long time is created, and excellent cell resources are provided for commercial production of cultured meat.

The present application discloses a cell culture unit, device, application and culture method, wherein the cell culture unit includes a substrate, a culture chamber, a liquid storage chamber and a flow channel chamber are formed in the substrate, and the culture chamber and the liquid storage chamber have an opening at the top of the substrate, and the two ends of the flow channel chamber are connected to the culture chamber and the liquid storage chamber respectively, and the flow channel chamber includes a first port portion connected to the liquid storage chamber and a second port portion connected to the culture chamber, and the flow channel chamber is also formed with a constriction portion between the first port portion and the second port portion, and the opening area of ​​the constriction portion is smaller than the opening area of ​​the first port portion and the second port portion. The technical solution of the present application can avoid the impact on the biological sample caused by too fast flow rate, resulting in the cells in the liquid gel being dispersed by the culture medium, and increase the time for the culture medium to infiltrate the biological sample to fully infiltrate the biological sample, thereby being able to improve the simulation degree of the cell model finally obtained and improve the success rate of culture.

Embodiments of the invention include additional methods and compositions for fabricating organoids. A unique approach has been developed for the rapid aggregation of defined number of cells using a micropillar-based template to limit the size of organoids, which are then more commonly referred to as spheroids. This procedure can be utilized to create in vitro cardiac tissue models (e.g., cardiac organoids) simply, rapidly, and inexpensively. In certain aspects an alginate-gelatin based hydrogel premixed with cardiomyocytes is used in combination with a patterned microfluidic chip / cell culture chamber having an array of micropillars to fabricate 3D cardiac organoids.

A method and system to make a modular microfluidic device in a miniaturized form for cell culture, co-culture, and organ-on-a-microfluidic device as in-vitro model of human organs within compartmentalized 3D structures is described. The modular microfluidic device enables co-culturing of heterogeneous cell assemblies that are adjacent or sequentially connected to promote enhanced cell-cell interaction. In vivo-like flow into and / or from one cell growth chamber to adjacent cell growth chamber is maintained by micro-engineered porous barriers i.e., porous walls and membranes. These porous barriers provide a tool for mimicking the paracrine exchange between cells in the human body. The system comprises of a set of microelectrodes for real-time and long-term monitoring and quantitative measurement of the impact of stimuli on the epithelial tissue permeability. The modular microfluidic system provides a platform technology for bio-fabrication of variety of miniaturized in-vitro models for mimicking the structure and function of single and multi-organ models.

The invention relates to the technical field of microbial culture, and discloses a cell culture device, which comprises: a plate body; the culture units are arranged on the plate body at intervals in the width direction of the plate body, and each culture unit comprises a plurality of base holes which are arranged at intervals in the length direction of the plate body and are used for culturing cells and fluid channels which are arranged between every two adjacent base holes; according to the flowing direction of the culture medium along the fluid channels, the two sections of fluid channels corresponding to the two upward sides of each base hole are called the upstream fluid channel and the downstream fluid channel of the base hole; a downstream hole wall is arranged at least between the fluid channel at the downstream of the base hole and the base hole, and a plurality of downstream gaps allowing the culture medium to pass through and limiting the cells to pass through are formed in the downstream hole wall in a cutting manner.

The invention belongs to the technical field of cell culture, and particularly relates to an intelligent chimeric cell culture cabin which is characterized by comprising an outer cabin body and a culture mechanism mounted above the outer cabin body, the fan rotates to suck air outside the device into the culture cabin through the air inlet, and in the process of blowing the air into the culture cabin, the heating pipe can heat the sucked air to increase the temperature of the air, so that the temperature of the air sucked into the device adapts to the temperature required by cell culture, and the situation that cell culture is affected by improper temperature is avoided; the sucked air is discharged out of the device through the air outlet after being circulated in the culture cabin body, so that the air in the culture cabin body can be circulated, meanwhile, waste gas generated by culturing cells in the culture cabin body is taken away, and angle openings of the air dissipation openings are staggered, so that the air discharged from the air dissipation openings can face different angles, and the air dissipation effect is improved. And the situation that the blown airflow faces the same angle and is too intense is avoided.

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

The invention relates to a cell culture microcarrier as well as a preparation method and application thereof. The cell culture microcarrier comprises a polymer porous microsphere with an inside-outside through pore channel structure. The cell culture micro-carrier comprises a biodegradable micro-carrier and also comprises a non-degradable micro-carrier, so that the growth of cells in the carrier is facilitated, and the mass transfer of nutrient substances and metabolites is also facilitated, thereby promoting the growth of the cells, enabling the cultured cells to keep good activity and function, and being high in proliferation efficiency; the method is suitable for three-dimensional culture of somatic cells, stem cells, tumor cells and the like.

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention discloses hyaluronic acid / alginic acid dual-network hydrogel as well as a preparation method and application thereof. The hyaluronic acid / alginic acid double-network hydrogel comprises a first network and a second network which are not crosslinked with each other, and the first network and the second network are distributed in the hydrogel in an interpenetrating manner; the first network comprises an ionic cross-linked network of alginic acid with cell adhesion sites and calcium ions, and the second network comprises a chemical cross-linked network formed by covalent cross-linking of methacrylic anhydride modified hyaluronic acid. The hyaluronic acid / alginic acid double-network hydrogel disclosed by the invention is short in curing time, supports three-dimensional spreading of cells and formation of capillary lumens, has good mechanical stability, and has good application prospects in the aspects of three-dimensional culture of the cells, tissue engineering construction and the like.

The invention belongs to the field of microbial culture, and particularly relates to microbial cell culture equipment which comprises an incubator main body, an oscillation platform is arranged in the incubator main body, the front end of the incubator main body is rotatably connected with an incubator door, the rear end of the incubator door is provided with a sliding mechanism, the sliding mechanism comprises two groups of first connecting rods, and the bottom ends of the first connecting rods are rotatably connected with connecting assemblies; a second connecting rod is rotationally connected to the bottom end of the connecting assembly, a pulley is fixedly connected to the side, close to the moving platform, of the second connecting rod, and the pulley is slidably connected to the interior of the sliding groove. Through the structural design of the sliding mechanism, when the incubator door is pulled to open the incubator main body, the first connecting rod, the second connecting rod and the connecting assembly at the rear end of the incubator door can take out the moving platform on the oscillating platform, so that the operation does not need to be performed inside the microorganism culture when the culture container is taken out.

The invention discloses a serum-free adipogenic differentiation method for porcine adipose precursor cells, and relates to the technical field of mammalian animal cell differentiation.In the adipogenic differentiation process, an induced differentiation culture medium and a maintained differentiation culture medium are sequentially used, and the induced differentiation culture medium is composed of a basic culture medium and cell culture auxiliary factors; the cell culture auxiliary factor is composed of 66nmol / L insulin, 33 [mu] mol / L biotin, 17 [mu] mol / L pantothenic acid, 0.1 [mu] mol / L dexamethasone, 0.25 mmol / L IBMX, 1 [mu] mol / L rosiglitazone, 150 [mu] mol / L sodium oleate and 10 [mu] g / mL transferrin; the maintenance differential medium removes IBMX and rosiglitazone on the basis of inducing the differential medium. The serum-free adipogenic differentiation method for the porcine fat precursor cells, provided by the invention, is good in differentiation effect and high in repeatability.

The invention belongs to the technical field of bioengineering, and particularly relates to a bioengineering-based cell culture environment simulation system, which comprises an incubator device and a control system for controlling the incubator device, and the control system is used for controlling the incubator device to complete simulation of a temperature and humidity environment required by cell culture; the control system comprises an acquisition module, a control module and a processing module, the acquisition module comprises a temperature sensor and a humidity sensor which are arranged in the incubator, the control module comprises a temperature adjusting mechanism and a humidity adjusting mechanism which are arranged in the incubator, and the processing module comprises a processing chip and an upper computer. Firstly, constant temperature and humidity required by cell culture are input through an upper computer, a decoupling control method is operated in the upper computer, and the upper computer controls a temperature adjusting mechanism and a humidity adjusting mechanism to work simultaneously through the decoupling control method to complete simulation of a cell culture environment. The coupling effect between temperature and humidity adjustment can be reduced.

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

To provide a waste liquid withdrawing system that can smoothly withdraw waste liquid without scattering the liquid, and a cell culture system.SOLUTION: A waste liquid withdrawing system comprises: a waste liquid withdrawing mechanism which withdraws culture solution not in use anymore from a plurality of flasks arrayed in a horizontal direction as waste liquid; and a recovery mechanism having a recovery container which recovers waste liquid withdrawn by the waste liquid withdrawing mechanism, where the waste liquid withdrawing mechanism comprises: a sucking mechanism which has a suction part that sucks waste liquid in the flasks, and leads waste liquid to the recovery mechanism; a support mechanism having an arm to which suction part is attached, which is provided movably in a vertical direction, and rotates about an axis line extending in a vertical direction to turn the suction part; a cleaning part which is arranged on a trajectory of the suction part viewed from a vertical direction, and reserves cleaning fluid; and a moving mechanism which linearly moves the plurality of flasks in a horizontal direction, and arranges one flask on a trajectory viewed from a vertical direction.SELECTED DRAWING: Figure 3

The invention discloses a preparation method and application of a cell culture liver based on a microcarrier. The method comprises the steps of obtaining liver seed cells, preparing the microcarrier, culturing cells in vitro based on the microcarrier, preparing the cell culture liver and the like. The preparation method of the cell culture liver based on the microcarrier is provided for the first time, and the preparation scheme that a cell-microcarrier compound serves as a structural unit and is assembled into the cell culture liver after being mixed with an edible material is adopted, so that on one hand, simulation of a real liver lobule structure can be achieved, and on the other hand, simulation of the liver lobule structure can be achieved; the whole structure relation of liver lobules and connective tissues in liver tissues can be simulated, full reduction of animal liver appearance and structural characteristics is achieved, edible materials used in the preparation process are low in price and good in biocompatibility, and the preparation method is suitable for large-scale industrial production. Liver seed cells can be subjected to efficient amplification and albumin synthesis through three-dimensional culture on a microcarrier prepared on the basis of edible materials, so that the cell culture liver is close to a real animal liver in the aspect of nutrition.

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

The invention provides cell culture monitoring equipment which comprises a cell culture box and a monitoring module, the cell culture box is used for installing a micro-fluidic chip and further comprises a first observation window, the first observation window is used for exposing an observation area of the micro-fluidic chip, and the monitoring module comprises an imaging assembly. The imaging assembly is used for collecting an image of the micro-fluidic chip from the first observation window. The cell culture monitoring equipment can realize real-time and online monitoring of the cell growth process.

The invention provides a mesenchymal stem cell culture monitoring system based on multiple parameters, and relates to the technical field of data processing, and the mesenchymal stem cell culture monitoring system comprises a first-level control instruction generation module which is used for generating a second-level control instruction when the metabolism-morphological abnormality association intensity in a combined fuzzy state matrix is judged to reach a preset association threshold value through a fuzzy rule base; when the abnormal state flag bit indicates that the isolated metabolism is abnormal, a second-level control instruction is generated; the driving unit is used for responding to the first-stage control instruction and cooperatively driving the buffer solution injection unit and the nutrition supplementing pump; responding to the secondary control instruction, and dynamically adjusting the opening degrees of a stirring rate controller, a temperature feedback loop and a gas proportional valve of the bioreactor. The control efficiency and the cell culture stability are improved.