8 results about "Dextranase" patented technology

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention discloses a high-activity dextranase preparation system and a high-activity dextranase preparation method. The high-activity dextranase preparation system comprises a fermentation device and a purification device, the fermentation device comprises a fermentation tank, a monitoring mechanism, a material supplementing mechanism, a continuous flow circulating mechanism, a microfluid impedance spectroscopy analysis mechanism and a control mechanism, the monitoring mechanism is connected to the fermentation tank, and the material supplementing mechanism is connected to the fermentation tank and used for supplementing liquid into the fermentation tank; the continuous flow circulating mechanism is connected between the bottom and the top of the fermentation tank so as to circularly process fermentation liquor in the fermentation tank, and the microfluid impedance spectroscopy analysis mechanism is connected to the continuous flow circulating mechanism so as to collect a metabolic flow characteristic frequency spectrum; the control mechanism can execute a self-adaptive pulse material supplementing algorithm according to the metabolic flow characteristic frequency spectrum and control the material supplementing mechanism to supplement materials, and the purification device performs purification treatment on fermentation products from the fermentation tank through a nine-time filtration purification process. The method improves the purity, improves the enzyme activity, improves the production efficiency, and reduces the cost.

The present invention aims to provide a liquid oral composition that does not contain ethyl alcohol and can sufficiently suppress the formation of precipitates during high-temperature storage. [Solution] The present invention provides a liquid oral composition that is substantially free of ethyl alcohol and comprises (A) component: dextranase and (B) component: xanthan gum, preferably further comprising (C) component. It is preferable that the content of component (A) is 0.0077 to 1.54% by mass, the content of component (B) is 0.01 to 1.0% by mass, and the mass ratio of the content of component (A) to the content of component (B) ((A) / (B)) is 0.6 to 150.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".

This invention discloses a method for preparing recombinant dextranase from Chaetomium globosum and its uses. The amino acid sequence of the recombinant dextranase is shown in SEQ ID NO.2 or SEQ ID NO.4, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1 or SEQ ID NO.3. The optimal pH of the recombinant dextranase is 4.5-7.0, and the optimal reaction temperature is 45-60℃. + K + and Ca 2+ It has an activating effect on enzyme activity, Mg 2+ NH4 + and Mn 2+ It had no significant effect on enzyme activity, Fe 3+ Zn 2+ Cu 2+ and Co 2+ It has an inhibitory effect on enzyme activity. Furthermore, this enzyme exhibits excellent pH stability, good thermal stability, and anti-interference ability, making it well-suited to the application requirements of the food, pharmaceutical, and chemical industries.

The present application relates to a kind of inhibiting dental plaque and composition of calculus, belong to oral care technical field.It contains composite plant fermentation extract, immobilized lysozyme, immobilized glucose oxidase, dextranase, chelating agent and oral acceptable carrier according to certain mass fraction;Composite plant fermentation extract is made by being fermented by specific concentration lactobacillus plantarum after being mixed by salvia miltiorrhiza and rhodiola rosea powder according to 3-5:1.The composition is synergized by composite plant fermentation extract and immobilized enzyme preparation, chelating agent, not only improves the storage stability and action efficiency of enzyme, realizes the source inhibition of dental plaque formation and the hindering of calculus mineralization, but also has additional functions such as oral mucosa protection, anti-inflammatory, solves the problem of single function, insufficient stability, low active ingredient utilization rate of existing product, and can be used for preparing toothpaste, mouthwash and other oral care products.

This invention provides a marine-derived glycomonad K1 strain, a method for producing dextran anhydrase, and its applications. The strain is a Gram-positive actinomycete. Colonies on a solid culture medium containing blue dextran exhibit the following characteristics: initially smooth surface, turning into a white, dry powder after three days of incubation; dried, with radially arranged edges; white on the front and black and opaque on the back. This invention also provides a method for producing dextran anhydrase using the aforementioned glycomonad K1 strain. The dextran anhydrase prepared using this method exhibits good thermal stability and strong pH tolerance, and can be widely used in the sugar, food, pharmaceutical, health, daily necessities, and chemical industries.

The present invention aims to provide a liquid oral composition that contains no ethyl alcohol and in which the generation of precipitates during storage at high temperatures can be sufficiently suppressed. Specifically, the present invention provides a liquid oral composition that is substantially free of ethyl alcohol, and comprises dextranase as a component (A), and xanthan gum as a component (B), and preferably further comprises a component (C). It is preferable that at least one of the following conditions is satisfied: the content of component (A) is 0.0077 to 1.54% by mass; the content of component (B) is 0.01 to 1.0% by mass; and the mass ratio ((A) / (B)) of the content of component (A) to the content of component (B) is 0.6 to 150.