232 results about "Dextranase" patented technology

Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.

The invention discloses a composite plant enzyme containing probiotics and an application of the composite plant enzyme. The composite plant enzyme comprises amylase, lipase, protease, cellulase, beta-dextranase, xylanase and lactase. The composite plant enzyme is obtained by discontinuously feeding materials and fermenting for a plurality of periods, selecting a plurality of types of fruits and vegetables, and edible and medical plants as fermentation substrates and taking a plurality of types of probiotics / yeasts as fermentation inoculation substances through a plurality of grades of deep fermentation. The composite plant enzyme contains seven enzymes needed by a human body at the same time so that the plant enzyme has the synergistic effects of promoting in-vivo metabolism and decomposing toxic substances; the plant enzyme contains a high-activity compound enzyme and has a wide acting condition, good adaptability, and good stability, acid-base resistance and high-temperature resistance; the plant enzyme can effectively and rapidly improve the biochemical reaction speed and can be used as a raw material to be applied to foods and chemicals for daily use.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The invention discloses a complex enzyme preparation for a growing and fattening pig feed. The complex enzyme preparation for the growing and fattening pig feed comprises the following eight enzymes: acid protease, amylase, beta-mannase, xylanase, beta-dextranase, cellulase, pectinase and phytase; and the enzyme activity ratio of the eight enzymes is sequentially 1: (0.8-14.5): (0.19-0.3): (33.7-35): (25-30): (3.2-3.5): (0.19-2.0): (0-0.025). The complex enzyme preparation can degrade various anti-nutritional factors in the feed, reduce the chime viscosity of the intestinal tract, improve the metabolic energy of the feed, improve the digestion rate of growing and fattening pigs on protein and starch, reduce the nutrient content of the chime entering the intestinal tract, effectively inhibit the reproduction of harmful microbes in the intestinal tract, reconcile the micro-ecological balance of the intestinal tract, promote the health of the pigs, improve the average daily gain of the growing and fattening pigs, reduce the feed-meat ratio, shorten the maintenance period and improve the economic benefit.

The invention relates to a multi-enzyme preparation capable of effectively reducing malt and beer turbidity, which is characterized in that the multi-enzyme preparation comprises the following ingredients in percentage by weight: 15 to 20 percent of hemicellulase, 10 to 25 percent of beta-dextranase, 15 to 30 percent of prolease, 5 to 15 percent of alpha-amylase and 10 to 25 percent of pure water. The multi-enzyme preparation belongs to the multi-enzyme preparation capable of effectively reducing the malt and beer turbidity, and the turbidity of partial finished products of malt is obviously higher because the varieties, the production places or the production processes of barley are different. The multi-enzyme preparation can effectively solve the problem that the turbidity of the malt is obviously higher, the contents of macromolecular substances such as beta-dextranase, xylan, protein, starch and the like are reduced, the abiological stability of the beer is improved, and the quality of products is improved.

The invention discloses a feed compound enzyme-containing dedicated enzyme for growing pigs and a preparation method of the feed compound enzyme-containing dedicated enzyme, and belongs to the technical field of preparation of enzyme preparations. The dedicated enzyme for the growing pigs is prepared by scientifically compounding aspergillus niger cultures, acidic xylanase, phytase, beta-dextranase, cellulase, amylase, acid proteinase, Chinese herb extracts, a protective agent and an activating agent, wherein the aspergillus niger cells in the aspergillus niger cultures can inhibit the growth of pathogenic escherichia coli, inhibit the growth and breeding of aspergillus flavus, disintegrate the aspergillus flavus, promote the growth of animals, adjust gastrointestinal tract flora to be balanced, and enhance whole immunity during the processes of growth and fermentation, in addition, the fermentation liquid crude enzyme liquid of the aspergillus niger cultures contains different enzyme activity such as protease activity, mannase activity, alpha-galactase activity and pectinase activity, and the dedicated enzyme is specially suitable for being added in the feed for the growing pigs.

The invention relates to an exogenous composite enzyme preparation special for feed industry and a preparation method and application thereof. The composite enzyme preparation comprises five kinds of water-soluble non-starch polysaccharide enzyme and phytase, namely xylanase, dextranase, mannose, cellulase and pectase. The activity mixture ratio of the contained water-soluble non-starch polysaccharide enzyme to the contained phytase is designed according to the kinds and the contents of the non-starch polysaccharide and the phytase in raw materials of a common feed, the synergistic effect between two kinds of the enzymes can be fully achieved, and the most scientific and economic mixture ratio is provided. The exogenous composite enzyme preparation can improve the degradation efficiency of non-starch polysaccharide anti-nutritional factors and phytic acid in the feed, remove the anti-nutritional effect thereof, improve the absorption and conversion rate of the feed and the utilization rate of phosphorus in the feed, substantially reduce the dosage of inorganic phosphorus and the production cost, bring considerable economic benefit to feed processing enterprises and farmers, greatly reduce the contents of nitrogen and phosphorus in animal excrement so as to reduce environmental pollution, and has remarkable social benefit.

The invention discloses a method for preparing chitosan oligosaccharide by degrading chitose comprising steps removing extraneous constituents through fermentation culturing and separation purifying by utilizing shell dextranase bacillus TQK to obtain TQK enzyme liquor which enzyme activation is up to 718u / ml, putting TQK enzyme liquor into 10-20poncentration degrading liquor which uses chitose as bottom liquor, the ratio of TQK enzyme liquor to bottom object is 1:10, and the enzymolysis reaction condition is: mixing speed is 200-300 r / min, Ph is 4-5.5, temperature is 45-52 DEG C and time is 4-6 hours, then centrifugal separating, flocculating, fining, filtrating, ion exchanging, hyperfiltration stage treating, vacuum concentration disposing, sponging drying disposing to prepare chitosan oligosaccharide. The enzymolysis bubatrate concentration can be up to 15-20, enzymolysis time is only 4-6 hours, receiving rate is high, energy consumption is low, pollution is small, also the invention can prepare narrow molecular weight distribution chitosan oligosaccharide with average molecular weight D1500 in quack speed, low consumption and large mass.

The present invention relates to enzyme preparation, and is especially one kind of composite enzyme preparation for degrading agricultural waste effectively and its preparation process. The composite enzyme preparation includes xylanase with enzyme activity not lower than 100 KU / g, beta-dextranase with enzyme activity not lower than 80 KU / g, cellulose with enzyme activity not lower than 28 KU / g, acid protease with enzyme activity not lower than 56 KU / g, lipase with enzyme activity not lower than 15 KU / g, pectase with enzyme activity not lower than 6 KU / g, and alpha-amylase with enzyme activity not lower than 12 KU / g. Its preparation process includes the steps of spawn preparation, enzyme preparation production, compounding, etc. The present invention may be used in feed production, methane production and other fields.

The invention discloses a feed for improving pig growth, which consists of the following components in parts by weight: 725 to 250 parts of soybean meal, 100 to 150 parts of peanut meal, 25 to 30 parts of imported fish meal, 25 to 30 parts of bean oil, 16 to 17 parts of common salt, 38 to 40 parts of calcium hydrogen phosphate, 2 to 3 parts of choline, 1 to 2 parts of threonine, 14 to 15 parts oflysine, 10 to 15 parts of complex microelement, 10 to 15 parts of complex vitamin and 2 to 2.5 parts of enzyme preparation, wherein the enzyme preparation consists, in parts by weight, of 400 to 600 parts of xylanase, 85 to 105 parts of beta-dextranase, 23 to 43 parts of cellulose, 105 to 125 parts of mannase and 44 to 640 parts of phytase. The feed for improving pig growth of the invention can raise the multiplication capability of growing pigs, lower cost in feed, improve intestinal health and result in fresh and good-quality slaughtered pork.

Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.

The invention relates to a double enzyme method for preparing medicinal dextran with controllable molecular weight. The medicinal dextran comprises dextran 70, dextran 40 and dextran 20. The preparation steps of the medicinal dextran comprise two steps of: firstly, synthesizing high molecular dextran: firstly, preparing the high molecular dextran by catalyzing sucrose by adopting dextran, wherein the conversion rate of the sucrose converted into the dextran reaches over 95 percent; secondly, controllably degrading the high molecular dextran: carrying out catalytic degradation on the high molecular dextran into the medicinal dextran as the medicinal dextran by adopting dextranase with high enzyme activity and realizing the controllable degradation of the dextran; and after the reaction is ended, removing impure protein in the products by adopting a simple filtering method and obtaining dextrans with different molecular weights by adopting graded ethanol precipitation. The double enzyme method has the advantages of greenness, environment friendliness, low cost, simplicity and convenience; and in addition, according to the double enzyme method, the yield of products can be greatly improved and the medicinal dextran with high quality is obtained.

The invention discloses a growing-finishing pig later-stage microorganism fermentation antibiotics-free feed, which is formed by mixing batch and compound fermentation materials. Weight ratio of the batch and the compound fermentation materials is 92-96:4-8, and the compound fermentation materials are formed by compounding the fermentation materials parts by weight, lactobacillus acidophilus fermentation material 2.5-5.5 parts, bacillus subtilis fermentation material 0.05-0.1 parts, saccharomyces cerevisiae fermentation material 0.50-1.85 parts, trichoderma longibrachiatum fermentation material 0.05-0.15 parts, aspergillus niger 0.05-0.2 parts and aspergillus oryzae fermentation material 0.05-0.2 parts. The growing-finishing pig later-stage microorganism fermentation antibiotics-free feedis many in beneficial bacterium variety and large in number and simultaneously contains a plurality of high-activity digestive ferment including beta-dextranase, xylanase, cellulose, protease and thelike, thereby having good synergy function, being capable of improving immunity of organism of animals and improving microecology environment, and further improving digestion using rate of the feed and enhancing economic benefit of cultivation and quality of pork.

The invention discloses a method for improving starch yield in a corn starch processing process by using a complex enzyme preparation, which is characterized in that the complex enzyme preparation is cellulose, xylanase, pectinase, beta-dextranase, a stabilizer sodium chloride and a carrier corn starch. In the processing process for producing the corn starch by taking the corn as a raw material, the complex enzyme preparation is added to the fiber washing procedure. The yield of the starch is improved by the procedures of washing and drying the fiber, separating and drying corn protein, soaking and crushing corn, and separating and washing corn germs; and the adding amount of the complex enzyme preparation is 0.01-0.15 per mill of the weight of the corn. According to the method disclosed by the invention, on the basis of a traditional wet processing process, the enzyme preparation is added in the corn separating process, so that the mechanical separating efficiency is improved, and thus the yield of the corn starch and corn protein powder is effectively improved; and meanwhile, energy consumption can be effectively reduced.

The invention relates a method of for preparing agrocybe cylindracea hydrolysate by using a compound enzyme method, comprising the following steps of: (1) washing agrocybe cylindracea, crushing and pulping; (2) adding water for mixing pulp according to the proportion that the agrocybe cylindracea to water is 1:2 to 1:5; (3) first enzymolysis: adding compound enzyme I which comprises cellulase, xylanase, beta-dextranase, mannase and chitinase, wherein the addition amount of the compound enzyme I is 0.05% to 0.3% of that of the agrocybe cylindracea, and carrying out enzymolysis for 2-4 hours at 45 DEC C to 55 DEG C; (4) second enzymolysis: adding compound enzyme II and carrying out enzymolysis for 2-4 hours at 50 DEC C to 60 DEG C; (5) boiling enzymatic hydrolysate and killing enzyme; and (6) adsorbing, filtering and concentrating to obtain transparent hydrolysate. In the method, a compound enzyme preparation is used for preparing the agrocybe cylindracea so as to fully give play to the synergistic interaction; and a subsection processing technology is adopted for leading bioactive substances in the agrocybe cylindracea to be released to the greatest extent.

The invention provides a malt preparation method which can lower the content of beta-hyskon in malt. The method mainly comprises the following steps: A. malt immersion: a small amount of NaOH can be added to lower active levels of microbes, and gibberellic acid is added to treat barley, which can further speed up the related enzymic reaction, and the ventilation and the oxygen supply must be paid attention in the process of the malt immersion and the germination, which facilitates the generation of beta-dextranase, endopeptidase and alfa-amylase; B. germinating: firstly intermediate temperature air, secondly high temperature air and thirdly low temperature air are continuously transferred to supply fresh air; and C. drying: the low temperature long-time moisture expelling method adopted can continuously exert effects of the beta-dextranase and other enzymes. In the invention, three steps of preparing the malt are organically combined, all indexes of the malt are in a proper range, so that the high quality malt which has the advantages of lower content of beta-hyskon and good indexes of leaching rate, ammonia nitrogen and glycation rate, etc. can be prepared.

The invention discloses an enzymolytic preparation method for glossy ganoderma amylose which comprises the steps of, employing inclined-plane bacterial gradual cultivation to obtain the glossy ganoderma fungi fermentation liquid, adjusting fermentation liquid pH and temperature, carrying out enzyme decomposition to the glossy ganoderma mycelia by means of self enzyme group existed in glossy ganoderma mycelia, charging into the fermentation liquor by cellulase, beta-dextranase, pectin enzyme and neutral proteinase, carrying out hydrolytic decomposition, enzyme eradication, filtering, concentrating, alcohol depositing and separating.

The invention discloses a preparation method of a Tremelle fuciformis extract. The method comprises the following steps of: a. soaking Tremelle fuciformis with hot water, adjusting pH to 2.5-3.5, and carrying out a constant-temperature reaction at 85-95 DEG C, wherein the weight ratio of Tremelle fuciformis to water is 1:(5-20); b. treating with an enzyme after the constant-temperature reaction, and controlling pH to 3-6 and the temperature to 40-55 DEG C, wherein the enzyme is one of cellulase, pectinase, and dextranase; c. filtering after the enzyme treatment reaction: firstly performing pumping filtration by using a gauze with 300-400 meshes at 40-50 DEG C to obtain a filtrate; d. concentrating the filtrate, and preforming heat preservation and sterilization at 85-95 DEG C for 0.5-1 hour. According to the method provided by the invention, Tremelle fuciformis is soaked by using hot water and then treated by using the enzyme to remove spore wall of Tremelle fuciformis, which is convenient for the deep extraction, and then treated Tremelle fuciformis is filtrated to obtain the final Tremelle fuciformis extract with an extraction rate as high as 85%.

The invention discloses a vibriosp S6-2(Vibriosp S6-2) CGMCCN0.4011. The growth temperaute of the strain ranges from 4 to 37 DEG C, 25 DEG C is the most preferable; the growth pH ranges from 6 to 11, 7.0 is the most preferable; and the concentration of NaCl for growth is 0.5% to 10%, 4% is the most preferable. The invention further discloses a method for producing ocean low-temperature dextranase by vibriosp and a low-temperature dextranase product produced according to the method. The low-temperature dextranase plays an important role in the aspects of prevention and treatment of dental caries, sugar industry, production of pharmaceutical dextran and the like.

The invention relates to a compound enzyme preparation for cane sugar. The feature is that it contains 14-18wt% amylase, 10-14wt% prectinase, 8-12wt% dextranase, 8-12wt% protease, 16-20wt% cellulose enzyme. It could effectively improve the quality of sugar liquid, lower material viscosity, enhance sedimentation and filtering effect and accelerate absorbance for confection mother liquor sugar. It has the double effect for improving white granulated sugar and the yield.

The invention discloses a poultry enzyme including neutral protease and a preparation method of the poultry enzyme, and belongs to the technical field of enzyme preparation. The poultry enzyme is formed by bacillus subtilis culture, acid xylanase, acid protease, phytase, beta-dextranase, cellulase, amylase, pectinase, Chinese herb extracts, a protective agent and an activating agent through scientific formulation, wherein the bacillus subtilis culture can be used for improving the immunity of the animal organism, promoting the development of immune organs, promoting the maturity of intestinal track structure and function of the animals, increasing daily gain of the animals and improving the forage conversion rate. The poultry enzyme provided by the invention provides security digestive enzyme for poultries, so that the digestion burden can be effectively reduced, the use rate and growth rate of the raw materials can be improved, and the environment is protected; meanwhile, the suitable amount of an activation agent can sufficiently activate the efficiency of the enzyme in the same conditions, so that the enzyme additive amount is saved. Moreover, the Chinese herb extracts prolong the expiration period of the composite enzyme through the scientific formulation, and also improve the immunity of the fed poultries.

The invention discloses a method for solving creaming down of green-tea concentrated solution by utilizing a compound-enzyme preparation. The method comprises the procedures of: by adopting green tea as a raw material, extracting, carrying out primary filtration, enzymolysis, refined filtration, concentrating, sterilizing and canning, wherein the enzymolysis comprises the following steps: cooling green-tea extracting solution obtained by primary filtration to be 40-45 DEG C, and adjusting pH of the extracting solution to be 4.5-5.0; adding the compound-enzyme preparation into the extracting solution, carrying out enzymolysis for 50-70 minutes, then cooling enzymatic hydrolysate to be 10-15 DEG C; and the weight percent of the enzyme preparation, the added amount and the content of soluble solids of the green-tea extracting solution is as follows: 0.1-0.4% of tannase, 0.8-4.0% of acid proteinase, 0.5-3.0% of pectinase and 0.4-2.0% of beta-dextranase. The method disclosed by the invention has the advantages that the content of the substances such as tea polyphenol, macromolecular protein, pectin and polysaccharide is reduced by utilizing a compound-enzyme enzymolysis technology so as to inhibit the generation of creaming down of the green-tea concentrated solution.

The invention provides a method for preparing a microbial-source adverse resisting agent. The method comprises the following steps: aspergillus niger thalli is mixed with water in the proportion of 1:5; after the aspergillus niger thalli is ground for homogenate by a high-shear colloid mill, an aspergillus niger mycelium body is broken; a tissue tissue is less than 5 mu m; aspergillus niger thalli homogenate liquid is heated to 55 DEG C; 1 percent of cellulase, 1 percent of pectinase, 2 percent of beta-dextranase and 2 percent of acid protease are added to the homogenate liquid; the materials are well mixed with the homogenate liquid, subjected to heat preservation and hydrolysis for 6 hours at 55 DEG C, heated to 100 DEG C, kept for 10 minutes for killing enzyme, cooled and then subjected to high-speed centrifugation via a tubular centrifuge at the rotating speed of 15,000 rpm; and centrifugal supernatant is taken on standby. According to the weight ratio of (50-60):(2-3):(1-2):(3-5), the supernatant, xylo-oligosaccharide, chitosan oligosaccharide and fulvic acid are well mixed so as to completely dissolve additive substances in the supernatant, are subjected to ultrahigh-temperature instantaneous sterilization at 142 DEG C for threes seconds, and then are canned aseptically, so as to be prepared into finished products.

The invention discloses a microbial fermentation antibiotic-free feed for pregnant swine, which is prepared by mixing auxiliary material and compounded fermentation material in a weight ratio of 92-96:4-8, wherein the compounded fermentation material is compounded from the following fermentation materials in parts by weight: 2-5.5 parts of lactobacillus acidophilus fermentation material, 0.05-0.1part of bacillus subtilis fermentation material, 1.00-2.85 parts of Saccharomyces cerevisiae fermentation material, 0.05-0.15 part of trichoderma longibrachiatum fermentation material, 0.05-0.2 part of aspergillus niger fermentation material and 0.05-0.2 part of aspergillus oryzae fermentation material. The microbial fermentation antibiotic-free feed for lean-fleshed pregnant swine contains a large amount of various beneficial bacteria and various of high-activity digestive enzymes such as beta-dextranase, xylanase, cellulose, protease and the like, and has good synergistic effect, can improve the immunity of an animal and intestinal tract microecological environment, and can further enhance the health condition of pregnant swine and the feed digestion and utilization rate; therefore the economic benefit of swine culture is improved.

The invention relates to complex enzyme and application thereof, which belongs to the technical field of the brewing of distillate spirits. The complex enzyme comprises the following enzyme preparations and yeasts: 200 to 225 U / g of saccharifying enzyme (wheat), 80 to 100 IU / g of pullulanase (wheat), 10 to 15 IU / g of acid protease (wheat), 15 to 20 IU / g of cellulose (wheat), 15 to 20 IU / g of beta-dextranase (wheat), 5 to 10 IU / g of beta-mannase (wheat), 10 to 15 IU / g of xylanase (wheat), 5 to 10 IU / g of phytase (wheat), 5 to 10 IU / g of pectase (wheat), 1.5 to 2 percent of high temperature resistant saccharomyces cerevisiae (W / W), and 1 to 1.5 percent of aroma-producing yeast (W / W). The complex enzyme is applied as an additive to the production of wheat type distillate spirits. The complex enzyme can increase fermentable sugar, improve the utilization rate of raw materials, and improve the distillation yield; at the same time, the complex enzyme releases and synthesizes more flavor substances, and has the advantages of improving the quality of the distilled spirits and increasing the high-quality product rate of xiaoqu distillate spirits and so on; and the complex enzyme can be widely applied to the production of wheat type distillate spirits.

The invention provides biological straw feed and a preparation method thereof. The method for preparing the biological straw feed comprises the following steps: mixing yeasts, lactic acid bacteria, crop straws, magnetic water and a magnetized compound enzyme system, thereby obtaining a mixture; and fermenting the mixture, thereby obtaining the biological straw feed. The method for preparing the magnetized compound enzyme system comprises the following steps: magnetizing cellulase, protease, amylase, dextranase, pectinase and ligninase in a permanent magnetic field, thereby obtaining the magnetized compound enzyme system. In the process of preparing the biological straw feed, the biological fermentation technology is combined with the magnetization technology, fermentation of the crop straws can be effectively realized, and the activity of the compound enzyme system in the crop fermentation process is prevented from being reduced, so that the protein conversion rate is high. Therefore, the nutritional ingredients of the crop straws are fully converted.

Disclosed is an enzyme combination and sterilization preparation having the broad-spectrum high efficiency sterilization function and the preparation method, which relates to the food safety and enzyme preparation technique field. The invention provides the enzyme combination and sterilization preparation which is combined by lysozyme, Beta-1, 3- dextranase, Beta-mannase, enzyme and neutral protein enzyme, and adopts the coating agent formulation of fucose, xanthan gum, sodium carboxymethylcellulose and pullulan to coat the combined enzyme. The invention adopts the multi-enzyme combination and coating technology to optimize the combination of the enzyme, meanwhile protecting the enzyme by the coating technology so as to effectively kill gram positive and negative bacteria with wide control spectrum and high efficiency, thus effectively killing pathogenic bacteria such as salmonella, Escherichia coli and golden yellow staphylococcus, and the invention and is a perfect alternative to antibiotics within feedstuff of pig and poultry for the preservation of animal and plant products. The coating protection promotes the resistance of enzyme towards temperature, oxidant and acid, thereby enhancing the sterilization effects under unfavorable circumstances and expanding the field of applications of enzyme fungicide.

The invention discloses a transgene saccharomy cescerevisiae for degrading cellulose to synthesize ethanol, and a construction method thereof. The transgene saccharomy cescerevisiae is obtained through the following steps that: genes of three enzymes in a cellulose system are introduced into a saccharomy cescerevisiae together through a saccharomy cescerevisiae expression vector; the three enzymes in the cellulose system are endo beta-1, 4-dextranase, exo beta-1, 4- dextranase and cellobiase; the constructed transgene saccharomy cescerevisiae can effectively, massively and simultaneously express the three cellulases, and degrade the cellulose into glucose, combining with the inborn ability of the saccharomy cescerevisiae of synthesizing the ethanol by use of the glucose. Therefore, the transgene saccharomy cescerevisiae is capable of producing the ethanol by fermenting the cellulose, can produce the ethanol by taking agricultural wastes full of cellulose as raw materials, which not only dissolves the pollution caused by the agricultural wastes, but also produces fuel ethanol to form sustainable energy reservation, thereby having inestimable functions to alleviate the shortage of oil resource, guarantee the safety of national energy, and improve the pollution of automobile exhaust.

The invention relates to a producing strain of Beta mannanase. The classification and nomenclature of the producing strain of Beta mannanase is Bacillus subtilis with accession number of CGMCC No.2077. The most suitable pH and temperature for the glucanase produced by the producing strain of Beta mannanase of the invention are respectively 6.0 and 70 DEG C, enzyme activity changes little under the temperature of 50-70 DEG C and the rest enzyme activity is 20 percent after the glucanase being treated for 5 hours under the temperature of 60 DEG C. The invention has fairly good heat resistance and research value.

The invention provides a composite microbiological antibiotic and a preparation method thereof. The preparation method comprises the steps of respectively culturing lactobacillus rhamnose (A) and lactobacillus acidophilus (B) in an MRS culture medium for 48 hours at 37 DEG C, centrifuging the fermentation liquid at a high speed, adding solid ammonium sulphate into the supernatant, dissolving the generated precipitate in distilled water, purifying the precipitate by gel chromatography and performing freeze dehydration to obtain lactobacillus bacteriocin, adding water in Aspergillus niger thallus based on a ratio of 1:6, milling and crushing by colloid to form homogenate, heating to 55 DEG C, adding 1.5% of cellulase, 1.5% of pectinase, 2% of beta-dextranase and 2% of acid prolealytic enzyme, and mixing uniformly, maintaining temperature at 55 DEG C and hydrolyzing for 6 hours, killing enzyme for 10 minutes at 100 DEG C, after cooling, taking the centrifugal supernatant and performing freeze dehydration to obtain enzymolysis frozen dry powder (C) of mycelium content, and finally uniformly mixing bacteriocin A, bacteriocin B and C in a weight ratio of (15-20):(18-25):(20-35) to obtain the finished product.