The invention discloses a poultry enzyme including neutral protease and a preparation method of the poultry enzyme, and belongs to the technical field of enzyme preparation. The poultry enzyme is formed by bacillus subtilis culture, acid xylanase, acid protease, phytase, beta-dextranase, cellulase, amylase, pectinase, Chinese herb extracts, a protective agent and an activating agent through scientific formulation, wherein the bacillus subtilis culture can be used for improving the immunity of the animal organism, promoting the development of immune organs, promoting the maturity of intestinal track structure and function of the animals, increasing daily gain of the animals and improving the forage conversion rate. The poultry enzyme provided by the invention provides security digestive enzyme for poultries, so that the digestion burden can be effectively reduced, the use rate and growth rate of the raw materials can be improved, and the environment is protected; meanwhile, the suitable amount of an activation agent can sufficiently activate the efficiency of the enzyme in the same conditions, so that the enzyme additive amount is saved. Moreover, the Chinese herb extracts prolong the expiration period of the composite enzyme through the scientific formulation, and also improve the immunity of the fed poultries.