Process optimization method for producing dextran from beet

By optimizing the process of producing dextran from sugar beets, and utilizing pectinase pretreatment, compound enzyme catalysis, and multi-stage ethanol precipitation purification, the high cost and molecular weight distribution problems of the traditional sucrose fermentation method have been solved, achieving efficient and environmentally friendly dextran production suitable for pharmaceutical-grade products.

CN120944987APending Publication Date: 2025-11-14CHANGMAO BIOCHEMICAL ENG CO LTD
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Patent Information

Application Number
CN202511133945.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-14
Publication Date
2025-11-14

AI Technical Summary

Technical Problem

Traditional sucrose fermentation for the production of dextran suffers from high costs, difficulty in precisely controlling molecular weight distribution, high costs for treating fermentation waste liquid, and low equipment utilization.

Method used

An optimized process for producing dextran from sugar beets was developed, including pectinase pretreatment, compound enzyme catalysis, molecular weight regulation by endoglucanase, multi-stage ethanol precipitation purification, and resource utilization of Saccharomyces cerevisiae fermentation waste liquid.

Benefits of technology

It achieves a 60% reduction in raw material costs, precise control of molecular weight distribution, an 80% reduction in waste liquid treatment costs, improved equipment utilization, and product purity ≥98%, making it suitable for the industrial production of pharmaceutical-grade dextran.

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Abstract

The invention relates to the field of dextran production, and particularly discloses a process optimization method for producing dextran from beet, which comprises the following steps: pretreating beet juice by pectinase to obtain a high-purity substrate, innovatively adopting a dextransucrase and alpha-1, 3 branching enzyme compound catalytic system, and combining with a Mn < 2 + > cofactor and a Tween 80 stabilizer to improve the enzyme activity efficiency, thereby obtaining the high-purity dextran. The molecular weight distribution is regulated and controlled in real time through endoglucanase, and a multi-stage ethanol precipitation and purification process is matched, so that the purity of the product reaches 98% or above and PDIlt; 1.25, resource utilization of single-cell protein produced by waste liquid fermentation is originally realized, and the sucrose conversion rate of the whole process is gt; the recovery rate of dextran is greater than or equal to 85%. Compared with a traditional method, the method has the advantages of being low in raw material cost, high in molecular weight controllability, environmentally friendly and the like, and is suitable for industrial production of pharmaceutical-grade dextran.
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