385 results about "Continuous cropping" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses bacillus vallismortis LRB-5, a biocontrol microbial agent, a bacterial fertilizer and application of the bacillus vallismortis LRB-5, the biocontrol microbial agent and the bacterial fertilizer, the bacillus vallismortis LRB-5 is preserved in China General Microbiological Culture Collection Center on January 25, 2018, the preservation number is CGMCC No.15306, and the bacillus vallismortis LRB-5 is classified and named as bacillus vallismortis. The bacillus vallismortis LRB-5 provided by the invention can inhibit the growth of phytopathogen, promote the growth and development of arabidopsis thaliana and apples, and also can alleviate successive cropping obstacles of the apples.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention relates to a cultivation nutrient medium and a preparation method thereof. According to the nutrient medium, peat is completely abandoned, the coco coir, the straw and the biochar are combined to reduce ecological burden, the nutrient medium is an environment-friendly medium, the biochar fixes carbon and reduces emission, and the soil structure can be improved after long-term use; vermiculite improves the water-holding capacity, perlite and straw form a stable pore structure, and the ventilation porosity reaches 25% or above. Wormcast and a microbial agent synergistically release nitrogen, phosphorus and potassium, and the fertilizer efficiency is lasting. The microbial agent can inhibit soil-borne diseases such as fusarium and reduce the death rate of cultivated seedlings. The biochar adsorbs salt and toxins, and the EC value is stabilized at 1.2-1.8 mS / cm. The cost of the selected raw materials is reduced by 35% compared with that of a traditional substrate, and the substrate is suitable for large-scale production, suitable for vegetable seedling culture, potted flowers, organic farms and soilless culture systems, and especially suitable for saline-alkali soil or continuous cropping obstacle areas.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii BDLZ-3 and application thereof in relieving continuous cropping obstacles of crops. The invention discloses a strain of pseudomonas monteilii BDLZ-3, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.34349. The pseudomonas monteilii BDLZ-3 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated in the ginseng continuous cropping obstacle process can be efficiently degraded, the degradation rate of benzoic acid can reach 95.36%, various phenolic acids such as vanillic acid, cinnamic acid, palmitic acid and gallic acid can be effectively degraded, and the degradation spectrum is wide; when the pseudomonas monteilii BDLZ-3 is applied in a field, the content of benzoic acid in soil can be effectively reduced, meanwhile, the occurrence of ginseng root diseases can be controlled to a certain extent, the prevention and treatment effect reaches 41.23%, the growth indexes such as root length and root thickness are remarkably improved, and the yield increasing effect is achieved.

The invention belongs to the technical field of microorganisms, and particularly discloses a morchella growth-promoting strain bacillus MSB09, which is classified and named as bacillus sp. And has a preservation number of GDMCC No: 65938. Morchella is used as a precious edible and medicinal fungus, the planting scale is continuously enlarged, but the soil continuous cropping problem has become a main obstacle for soil-covered cultivation and fruiting of the morchella, the soil microorganism structure of the morchella is studied, and the morchella can be used for promoting growth of the morchella. And beneficial microorganism bacillus (Bacillus sp.) MSB09 is screened out, so that technical support is provided for reconstruction of soil micro-ecology, alleviation of continuous cropping obstacles of morchella esculenta and biological prevention and control.

The invention relates to the technical field of greenhouse vegetable planting environment regulation and control, in particular to an internet-based intelligent greenhouse vegetable environment regulation and control system and method, which comprises an acquisition module, a monitoring module, an analysis module, a decision module, an execution control module and a mobile terminal interaction module, in combination with a preset successive cropping obstacle threshold model, analyzing to obtain the soil secondary salinization degree, the nutritional imbalance state, the microflora disorder index and the allelochemical toxicity grade; and the decision module is used for generating a comprehensive regulation and control strategy including crop rotation suggestions, irrigation amount, a fertilization scheme, biological control measures and environmental parameter regulation instructions in combination with facility vegetable types and growth stage requirements. A continuous cropping obstacle analysis result is obtained through the analysis module in combination with big data and a machine learning algorithm, a comprehensive regulation and control strategy is generated, continuous cropping obstacles are effectively dealt with, and the planting efficiency and the vegetable quality are improved.

The invention discloses a special fertilizer composition for high-yield cultivation of sugarcane, and belongs to the technical field of sugarcane planting. Comprising a disease-resistant fertilizer and an insect-resistant fertilizer; wherein the disease-resistant fertilizer comprises the following raw materials in parts by mass: 50 parts of diatomite, 5-10 parts of biomass charcoal, 1-2 parts of a compound microbial agent, 150-170 parts of an organic fertilizer and 50-60 parts of an inorganic fertilizer; wherein the compound microorganism bacterium agent comprises a bacillus subtilis bacterium agent, a trichoderma harzianum bacterium agent, a trichoderma viride bacterium agent and a paecilomyces lilacinus bacterium agent; the insect prevention fertilizer comprises beauveria bassiana suspension and matrine in a volume ratio of (3.5-4.5): 1. According to the fertilizer composition special for high-yield cultivation of sugarcane, the synergistic effect of microorganisms is utilized, the broad-spectrum inhibition effect on microorganisms in sugarcane continuous cropping disease soil can be achieved, the kieselguhr and the biomass charcoal cooperate to form a porous substance, nutrient elements are adsorbed in pores, the slow-release effect is improved, the fertilizer efficiency is prolonged, and the effect is good. Moreover, the soil structure can be improved, and the disease resistance of the sugarcane during continuous cropping is greatly improved through the synergistic interaction with the microbial complex microbial inoculants.

The invention belongs to the technical field of microorganisms, and particularly relates to a Burkholderia sp. SJ08 strain, a Burkholderia sp. SJ08 fungicide, and a preparation method and application thereof. The invention discloses a strain of Burkholderia cepacia SJ08, the Burkholderia cepacia SJ08 is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC (China General Microbiological Culture Collection Center): 65908, and the preservation date is February 18, 2025. The Burkholderia sp. SJ08 strain is screened from seed radix pseudostellariae, crop diseases caused by fusarium oxysporum can be effectively inhibited, continuous cropping obstacles of crops can be inhibited, crop growth is promoted, and the Burkholderia sp. SJ08 strain is safe and free of pollution. The Burkholderia sp. SJ08 strain provided by the invention has a good application prospect in crop production.

The invention belongs to the technical field of plant growth-promoting bacteria, and particularly relates to protozoa trichomonad cooperating with bacillus to prevent and control cucumber fusarium wilt and application of the protozoa trichomonad. The invention provides a strain of protozoa trichomonas mtoleri (Cercomonas mtoleri) B5, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) N0: V202502. The protozoa trichomonad B5 provided by the invention can induce spore germination of a biocontrol strain bacillus velezensis SQR9 and improve the rhizosphere colonization capability of the biocontrol strain SQR9. The protozoa trichomonad B5 and the bacillus velezensis SQR9 are jointly inoculated in cucumber fusarium wilt continuous cropping soil, and the fusarium wilt morbidity of cucumber plants can be effectively reduced.

The invention discloses a rapid diagnosis method for blueberry continuous cropping soil obstacle factors, and belongs to the technical field of soil environment analysis. Based on combined analysis of soil physical and chemical factors, metagenome and metabolome, pathogenic bacteria types and key metabolites significantly related to the blueberry continuous cropping obstacle are extracted, and a characteristic diagnostic index system is formed. The index system comprises main pathogenic bacteria types, typical harmful metabolites, key soil physical and chemical factors and appropriate or risk threshold ranges thereof, and provides a scientific basis for rapid identification of soil continuous cropping obstacles. Comparative analysis is carried out by establishing a standardized index library, rapid comparison of related indexes and standard indexes in a newly sampled soil sample is realized, and whether the soil has continuous cropping obstacle risks or not is judged in an auxiliary manner by integrating the deviation degree of physical and chemical factors and biological factors.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention relates to the technical field of agricultural information and seed and seedling cultivation, and discloses a peanut planting method for relieving continuous cropping obstacles of peanuts. The method comprises the following steps: constructing initial digital twins of a land parcel, and synchronously constructing a disease-resistant peanut variety screening and seedling pretreatment system; historical continuous cropping information, soil physicochemical and microorganism data, meteorological time sequence data and seedling cultivation parameters are fused, the system is driven to evolve into operation state digital twins, and seedling-soil-environment collaborative simulation is achieved. Evaluating the continuous cropping obstacle risk and the seedling adaptability based on the twinborn body, and generating a comprehensive diagnosis report containing a nutrient imbalance index, pathogenic bacteria abundance prediction, a root exudates cumulative effect and a seedling disease-resistant adaptation coefficient; according to the method, differentiated seed and seedling optimization, soil improvement and cultivation strategies are implemented, and management measures are dynamically optimized through real-time data feedback in a growth cycle. Through cooperative driving of digital twinning and seed and seedling cultivation, source prevention, control and intervention of continuous cropping obstacles are achieved.

The invention relates to a growth-promoting compound microbial agent, which is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and is characterized in that the growth-promoting compound microbial agent is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and the growth-promoting compound microbial agent is prepared from bacillus amyloliquefaciens, bacillus subtilis and Trichoderma longibrachiatum. The compound microbial agent (bacillus subtilis, pseudofungal bacillus and trichoderma longibrachiatum) has excellent performance in the aspects of promoting plant growth and improving the soil environment, and the plant height, the fresh weight and the root length of plants are remarkably increased. The application of the complex microbial inoculant effectively relieves the chive continuous cropping obstacle, improves the chive growth potential and soil fertility, and provides a theoretical basis and technical support for sustainable planting of chives in facility agriculture.

The invention belongs to the technical field of microorganisms, and particularly discloses a morchella growth-promoting strain Clonostachys roseMSF05, the classification name is Clonostachys rosea, and the preservation number is GDMCC No: 65937, morchella is used as a precious edible and medicinal fungus, the planting scale is continuously enlarged, but the soil continuous cropping problem has become a main obstacle for soil-covered cultivation and fruiting of the morchella, the soil microorganism structure of the morchella is studied, and the morchella growth-promoting strain CClonostachys roseMSF05 can be used for promoting growth of the morchella. And the beneficial microbial strain clonostachys rosea MSF05 is screened out, so that technical support is provided for rebuilding soil micro-ecology, relieving continuous cropping obstacles of morchella esculenta and biological prevention and control.

The invention belongs to the technical field of biological bacterial fertilizers, and discloses a Chinese herbal medicine carrier-based multifunctional microbial bacterial fertilizer, which comprises a microbial strain group, a Chinese herbal medicine active component group, an organic matter and nutrition carrier, mineral elements and auxiliary components, and a functional synergist. Through the synergistic interaction of Chinese herbal medicine active ingredients and functional microbial flora, breeding of harmful bacteria in soil is inhibited, synthesis of acetylcholin esterase of nematodes is interfered, a continuous cropping disease occurrence chain is blocked from the source, and meanwhile, organic acid and flavonoid substances slowly released by a Chinese herbal medicine carrier are utilized to activate resistance gene expression of a plant system, so that the disease resistance of the nematodes is improved. The tolerance threshold of crops to high temperature, cold damage and diseases is enhanced; in the composite carrier, humic acid and diatomite are crosslinked through hydrogen bonds to form a stable granular structure, the cation exchange capacity and porosity of soil are effectively improved, the strong hydration capacity of polyglutamic acid is combined to lock nutrients and moisture, and development of meristem of a root system and proliferation of capillary roots are promoted.

The invention discloses beneficial fusarium oxysporum and application thereof in relieving apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the fusarium oxysporum FoB1 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.42195. The fusarium oxysporum FoB1 disclosed by the invention is beneficial fusarium oxysporum, and has the advantages of excellent biocontrol function, strong environmental adaptability and high affinity with a host. By optimizing the preparation process of the microbial inoculant, the activity and stability of the inoculant are remarkably improved, and a high-quality bacterium source is provided for large-scale production. On the basis, a simple and convenient bacterium-bearing nursery stock application technology is developed, a novel efficient biological prevention and control way is provided for relieving apple continuous cropping obstacles, and important technical support is provided for promoting green and sustainable development of the fruit tree industry.

The invention provides application of trichoderma in relieving continuous cropping obstacles of ginseng or salvia miltiorrhiza, and relates to the technical field of crop cultivation. Through deep research and creative labor, the trichoderma can effectively relieve soil chemical property deterioration and soil microorganism change after continuous cropping of the ginseng or the salvia miltiorrhiza, so that the soil chemical property after continuous cropping of the ginseng or the salvia miltiorrhiza can be improved, the relative abundance of beneficial bacteria in the soil is increased, the relative abundance of harmful bacteria is inhibited, and the yield of the ginseng or the salvia miltiorrhiza is increased. Meanwhile, the content of effective components of ginseng and salvia miltiorrhiza can be increased, and the quality of continuous cropping ginseng or salvia miltiorrhiza is improved.

The invention relates to the technical field of bio-organic fertilizers, in particular to a preparation method and application of a bio-organic fertilizer based on secondary fermentation propagation of bacillus velezensis, and the preparation method comprises the following steps: uniformly mixing livestock and poultry manure and straw powder, adding a high-efficiency decay-promoting microbial inoculum and a compound additive, and carrying out trough type turning, stirring and mixing to obtain the bio-organic fertilizer. A compost material after primary fermentation is obtained; and after the water content of the compost material after primary fermentation is reduced to 45-55% and the temperature is reduced to 45 DEG C or below, adding an amino acid raw material and a bacillus velezensis inoculant, stirring and mixing, continuing fermentation, controlling the temperature of the compost body for secondary fermentation to be less than or equal to 60 DEG C, stirring once a day, and naturally airing after fermentation is completed, thereby obtaining the biological organic fertilizer. According to the technical scheme, the effective viable count in the biological organic fertilizer and the soil colonization effect of the biological organic fertilizer are improved, so that the prevention and control effect on continuous cropping obstacle related pathogenic fungal diseases is improved, and an efficient and reliable technical scheme is provided for biological prevention and control of continuous cropping obstacles of traditional Chinese medicinal materials and greenhouse vegetables.

The invention relates to the technical field of facility agriculture, in particular to a green cultivation method for greenhouse water culture of Chinese chives. Through integration of intelligent greenhouse facilities, staged nutrient solution regulation and control, physical-biological-chemical synergistic disease and insect pest prevention and control and an annual production management technology, the problems of continuous cropping obstacles, high pesticide residues, winter dormancy and the like existing in traditional soil culture of Chinese chives are solved. According to the method, annual production of Chinese chives is achieved, the harvesting period of each crop is shortened to 20-30 days, compared with traditional soil culture, the annual yield is increased by 40% or above, the pesticide use amount is reduced by 95%, the pesticide residue percent of pass is 100%, and the product is free of heavy metal pollution, meets the green food standard and is suitable for green transformation and popularization of facility agriculture.

The invention discloses a planting method for overcoming continuous cropping obstacles of silage corn, which solves the problems of soil degradation and diseases and insect pests caused by continuous cropping by integrating soil physical remediation, chemical remediation, biological remediation, microbial community regulation and ecological intercropping technologies. The core technology content comprises the steps that deep ploughing is combined with organic matter and a biochar modifier to repair the soil structure, a compound microbial agent is sprayed to inhibit pathogenic bacteria, leguminous green manure and commercial crop rape are used for improving nitrogen circulation and reducing the autotoxicity effect every two years, and a precise fertilization management technology is matched. After the scheme is implemented, the soil health condition is remarkably improved, the silage corn yield is remarkably increased, and good ecological and economic benefits are achieved.

The invention discloses streptomyces obscura QS2-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces caniferus QS2-7 is obtained through separation, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.32328. Experiments show that the strain QS2-7 has a relatively strong inhibition effect on various plant pathogens, and has excellent biocontrol bacterium potential. And the strain has the functions of nitrogen fixation, phosphorus solubilization and protease production, and can effectively degrade autotoxic substances generated by plant planting and relieve autotoxicity. The strain can significantly promote plant growth and effectively reduce the incidence rate of diseases, and has high application value in green prevention and control of plant diseases and continuous cropping production.

The invention relates to the field of agricultural planting, in particular to a post-ginseng land soil improvement method based on compound bacteria fermentation degradation of allelochemicals. The invention provides a main technological process for improving soil through a fermentation method. The main technological process comprises the steps of land consolidation, ridge forming and pond building, material preparing and feeding and soil-material mixing, zymophyte inoculation and bacterium-material combination, film mulching anaerobic and fermentation monitoring, fermentation completion and post-treatment. The invention also provides a complex microbial inoculant composition capable of achieving the improvement effect, and the complex microbial inoculant composition comprises the following components in parts by weight: 5 parts of straw returning agent, 3 parts of organic fertilizer leavening agent and 1 part of bacillus subtilis agent. According to the method, the improved soil is subjected to long-time anaerobic fermentation in the water stain micro-ecological environment, the organic matter content of the soil can be greatly increased by adding a large number of organic matter materials, the carbon / nitrogen ratio of the soil is increased, the bulk density of the soil is reduced, fertility is comprehensive, sufficient and balanced, and the method is suitable for continuous cropping planting and healthy growth of ginseng.

The invention discloses mortierella alpina GRB-1, a fungicide and application of the fungicide, and belongs to the technical field of agricultural microorganisms. The Mortierella alpina GRB-1 is separated from root systems of healthy fruit trees in a continuous cropping apple orchard for the first time, and the Mortierella alpina GRB-1 has the capabilities of producing protease, producing siderophores, dissolving phosphorus, producing ammonia gas and producing auxin, can inhibit fusarium and has the application potential of relieving continuous cropping obstacles. The product prepared by combining the straw with crop straws has an excellent effect on relieving apple continuous cropping obstacles, can promote the growth of overground parts and underground parts of continuous cropping apple trees, improves the relative abundance of bacterial communities in apple continuous cropping soil and the nutrient content in the apple continuous cropping soil, improves the root activity of apples, and improves the survival rate of the apples. The method can be used for preventing and treating apple successive cropping obstacles.

According to the method, chewing cane ditching planting is adopted, the depth of chewing cane planting ditches is 20-45 cm, fresh corn is planted on ridge surfaces on the two sides of the planting ditches after chewing cane planting is conducted for 10-15 days, chewing cane-corn root system dislocation growth is achieved, root system antagonism between gramineous plants is avoided, ecological niche complementation is achieved, and the yield of the chewing cane is increased. The effective nutrient absorption space of crops is increased. After fresh corn ears and straws are harvested and sold, soil in a planting area is dug in time, furrows close to chewing cane planting rows are ridged to prevent falling, and the positions of ridges and furrows are interchanged. Position-changing rotation of the chewing cane and the fresh-eating corn every year is achieved, namely rotation of the fresh-eating corn is conducted in an original chewing cane planting area, and rotation of the chewing cane is conducted in an original fresh-eating corn planting area. Therefore, two planting modes of crop rotation and relay intercropping are combined, land resources are efficiently utilized, continuous cropping obstacles are avoided, the land output is increased, the land production cost is reduced, and good harvest of the chewing cane and the fresh corn is achieved.

The invention belongs to the technical field of agricultural fertilizers, and discloses a preparation process and application of a medical stone double-effect organic fertilizer with soil improving and growth promoting functions, the medical stone double-effect organic fertilizer comprises the following components by weight: 20-40 parts of activated medical stone powder with a particle size of less than or equal to 0.1 mm, and activated by a citric acid solution; the decomposed organic material is prepared by mixing livestock and poultry manure and crop straw according to the mass ratio of 2: 1-4: 1 and performing aerobic composting fermentation through a compound microbial agent, and the water content is smaller than or equal to 30%; the microbial agent contains phosphate solubilizing bacteria, nitrogen-fixing bacteria and rhizosphere growth-promoting bacteria, and the effective viable count is greater than or equal to 2 * 10 CFU / g; the auxiliary material comprises bentonite, a polyacrylamide water-retaining agent and a pH (Potential of Hydrogen) regulator, and the proportion of the bentonite is greater than or equal to 30 percent. The adsorption-desorption performance of the medical stone is enhanced by activating the medical stone through citric acid, and a synergistic system of water retention, fertilizer supply and growth promotion is formed by combining decomposed organic materials and functional microorganisms. According to the fertilizer, the stability of a soil aggregate structure can be remarkably improved (increased by 15-20%), the yield increasing rate of crops reaches 20-30%, and 30% of chemical fertilizer dosage is reduced. The method is suitable for acid soil remediation, continuous cropping obstacle treatment and economic crop planting.

The invention discloses a cultivation method for improving acidic soil of a greenhouse vegetable base based on rotation of morchella esculenta, and belongs to the technical field of soil improvement. The method comprises the following steps: (1) selecting an acidification test plot of long-term continuous cropping vegetables, and detecting soil pH, conductivity, water-soluble salt and anion and cation concentration; (2) preparing a morchella sextelata strain and culturing the morchella sextelata strain until the inoculation bag is full of hyphae; (3) spreading strains on the surface of soil, and managing fruiting after earthing; and (4) after harvesting morchella esculenta, detecting soil indexes, and evaluating the acidification improvement effect. According to the method, the soil pH value is remarkably increased through rotation of the morchella esculenta, the conductivity and the water-soluble salt content are reduced, the anion-cation balance is adjusted, the soil acidification problem caused by long-term continuous cropping of a greenhouse vegetable base is relieved, and meanwhile the land utilization economic value is increased.

The invention discloses a method for efficiently reducing nitrogen and phosphorus in greenhouse vegetable soil based on morchella esculenta cultivation, and belongs to the technical field of soil improvement and edible fungus cultivation. In order to solve the problem of soil nitrogen and phosphorus eutrophication caused by long-term continuous cropping of a facility vegetable base, by screening a specific Morchella sextella strain and combining with optimized strain culture, inoculation and field management technologies, the absorption efficiency of the Morchella sextella on the soil nitrogen and phosphorus is remarkably improved. The method specifically comprises the following steps: soil nutrient detection and eutrophication evaluation, directional culture of strains, layered soil covering and sowing, accurate supplementation of exogenous nutrition bags, and dynamic monitoring of hypha growth and nutrient absorption. Experiments show that the content of total nitrogen (TN) in soil can be reduced by 11.27%-18.01%, the content of total phosphorus (TP) can be reduced by 2.72%-6.16%, the reduction amplitude of rapidly available phosphorus (AP) can reach 2.12%-11.35%, and the accumulation of nitrogen and phosphorus in the soil can be effectively relieved. According to the method, a dual absorption mechanism is formed through hypha metabolism and sporocarp, rapid reduction of greenhouse vegetable soil nutrients is achieved, and a new way is provided for sustainable development of greenhouse agriculture.

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

The invention belongs to the technical field of soil improvement, and discloses a preparation method of a composite microbial agent and application of the composite microbial agent in continuous cropping stress resistance, the active ingredients of the composite microbial agent comprise Gliocladium mucilaginosum, Trichoderma harzianum and Pseudomonas chlororaphis, and a carrier contains a basic carrier, a synergistic component and a soil adaptive component (oyster shell powder or wormcast particles). The preparation method comprises the following steps: strain fermentation, carrier construction, solid-state co-fermentation, and mixing of a dried fermentation product and a carrier. After the compound microbial agent is used, the abundance of gliocladium mucilaginosum, trichoderma harzianum and pseudomonas chlororaphis in continuous cropping crop soil is obviously increased, and the abundance of fusarium oxysporum is obviously reduced; the acidification condition of the acidified soil is obviously improved, the content of organic acids (ferulic acid and vanillic acid) in the soil is obviously reduced, and the soil becomes loose from hardening, so that the root activity is improved, and the plant diseases and insect pests are resisted; the single fruit weight and sugar degree can be remarkably improved, the fruit storage rotting rate is reduced, and the effect is remarkable.