4 results about "Trichomonas" patented technology

The present application relates to the technical field of cell staining, and particularly relates to a vaginal secretion staining solution, a preparation method and a staining method thereof.The vaginal secretion staining solution provided by the present application uses basic fuchsin and methylene blue as staining agents, can stain epithelial cells and leukocytes, and cannot stain trichomonas and mold, so that leukocytes and trichomonas can be obviously distinguished through color difference, and personnel misjudgment caused by the similarity of leukocytes and trichomonas is reduced.The vaginal secretion staining solution provided by the present application has simple operation steps, short cycle and fast staining.The present application further provides a preparation method and a staining method of the vaginal secretion staining solution.

The application discloses an extractable nucleic acid trichomonad activity protection solution and relates to the technical field of biotechnology, which comprises two component reagents A and B, the reagent A comprises 0.8-1.0% NaCl, 0.1-5% Tris-Cl buffer, 0.1-1.5% SDS, 1.5-5% glucose, 5-15% serum, 0.5-5% betaine, and the balance is sterile deionized water, and the reagent B is a proteinase K solution. The application can not only dilute secretion, preserve trichomonas and culture trichomonas, but also be used for extracting trichomonad nucleic acid in PCR molecular experiment, so that the application can realize double application of trichomonad detection. The application not only helps to solve the health problems of women and improve fertility protection, but also is the first domestic invention to provide an example for new technology transformation service to the society.

The invention discloses a circular RNA (Ribonucleic Acid) and an application of the circular RNA in a trichomonad pigeon vaccine. The coding element contained in the circular RNA can be used for coding the recombinant trichomonas pigeon AP33 or AP65 protein. The coding element has a sequence as shown in SEQ ID NO.1 or SEQ ID NO.2 or a conservative variant sequence of the sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The circular RNA can induce protein expression in an animal body and generate a high-level antibody, does not contain infectious insect species components, can be degraded into nucleotides in the body and cannot be integrated into a host genome when being applied to a trichomonad pigeon vaccine, has no risk to pigeons in use, can stimulate the pigeons to generate relatively strong immune responses including humoral immunity and cellular immunity, and can be used for preparing a vaccine for preventing and treating the pigeons. Meanwhile, the vaccine is high in stability, can be prepared on a large scale through a molecular technology, and is low in production cost.