10 results about "Trichomonas" patented technology

The invention belongs to the technical field of plant growth-promoting bacteria, and particularly relates to protozoa trichomonad cooperating with bacillus to prevent and control cucumber fusarium wilt and application of the protozoa trichomonad. The invention provides a strain of protozoa trichomonas mtoleri (Cercomonas mtoleri) B5, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) N0: V202502. The protozoa trichomonad B5 provided by the invention can induce spore germination of a biocontrol strain bacillus velezensis SQR9 and improve the rhizosphere colonization capability of the biocontrol strain SQR9. The protozoa trichomonad B5 and the bacillus velezensis SQR9 are jointly inoculated in cucumber fusarium wilt continuous cropping soil, and the fusarium wilt morbidity of cucumber plants can be effectively reduced.

The invention discloses a recombinant subunit vaccine for resisting trichomonas pigeonae infection and a preparation method of the recombinant subunit vaccine. The vaccine comprises two recombinant proteins with sequences as shown in SEQ ID NO: 7 and SEQ ID NO: 8 respectively and a pharmaceutically acceptable carrier. According to the invention, Sf9 cells are used for respectively expressing trichomonas pigeonae adhesion protein 33 and adhesion protein 65, the immunogenicity of the obtained recombinant protein is similar to that of natural protein, the expression level is high, the immunogenicity is strong, a very small amount of recombinant protein can provide a better immune protection effect, and the recombinant protein has no pathogenicity to pigeons, and can be used for preparing the recombinant protein for preventing and treating trichomonas pigeonae. Meanwhile, large-scale serum-free suspension culture can be carried out through a bioreactor, so that the vaccine production cost is greatly reduced.

The present application relates to a kind of microbial microscopic image target feature identification method, device and storage medium, applied to image processing technical field, comprising: by marking the image on the target to be identified, convert the target to be identified into different scale space and construct multiple groups of Gaussian pyramid and convert into feature pyramid, according to feature pyramid, obtain feature point, obtain the feature parameter of each feature point, when the target needs to be identified, convert the image to be identified into multiple groups of Gaussian pyramid, and the pixel point in Gaussian pyramid is matched with feature point, if the number of pixel point on the image to be identified is matched exceeds the number of pre-set, then consider that there is identification target on the image;By the present application, without using convolutional neural network, microorganism image features can be quickly and accurately automatically identified, and then automatically identify white blood cells, clue cells, spores, blastospores, hyphae, trichomonas and other microorganisms.

The present invention relates to the technical field of molecular detection. Provided is a duplex nested PCR method capable of simultaneously detecting Pentatrichomonas hominis and Tetratrichomonas buttreyi. A positive control mixture of Pentatrichomonas hominis and Tetratrichomonas buttreyi is used as a template, and a Trichomonas genus-specific primer is used as an amplification primer to perform amplification; and an amplification product obtained from the first round of PCR is used as a template, and a mixture of a Pentatrichomonas hominis-specific primer and a Tetratrichomonas buttreyi-specific primer is used as a primer to perform amplification, thereby enabling simultaneous detection of Pentatrichomonas hominis and Tetratrichomonas buttreyi. The method can specifically amplify target fragments of Pentatrichomonas hominis and Tetratrichomonas buttreyi, and can detect positive control DNA at a minimum concentration of 500 fg / μL.

The application discloses a trichomonad detection device, method, equipment and computer readable storage medium, which is applied to the technical field of image processing and comprises the following steps: acquiring a gynecological microscope sample image sequence to be detected by using a gynecological microscope sample image sequence acquisition module; detecting the gynecological microscope sample image sequence to be detected by using a trichomonad detection network model to obtain a target recognition result of each frame of image; processing the gynecological microscope sample image sequence to be detected by using an optical flow algorithm to obtain optical flow data of each frame of image by using an optical flow data determination module; and determining a moving trichomonad recognition result according to the target recognition result of each frame of image and the optical flow data of each frame of image by using a moving trichomonad recognition result determination module. Compared with the current detection of trichomonads only according to artificial medical methods, the application can detect moving trichomonads from multiple aspects based on the combination of optical flow data and the target recognition result of each frame of image, and the probability of missed recognition of trichomonads is reduced.

The invention discloses a high-food-calling pet parasite expelling tablet and a preparation method thereof, and relates to the technical field of pet parasite expelling tablets, the high-food-calling pet parasite expelling tablet is composed of a traditional Chinese medicine extract, a tablet auxiliary material and a food-calling auxiliary material, the traditional Chinese medicine extract is composed of a quispualis indica extract, a betel nut extract, a dark plum extract, a cyrtomium fortunei extract, a radix stemonae extract and a fructus cnidii extract; the tablet auxiliary materials comprise maltodextrin, microcrystalline cellulose, corn starch, anhydrous calcium sulfate and magnesium stearate. According to the high-food-calling pet parasite expelling tablet and the preparation method thereof, the self-help feeding rate of pets reaches up to 90% or above, and the operation complexity of pet owners in the parasite expelling process is reduced; meanwhile, a good expelling and killing effect is achieved on various common parasites such as roundworms, tapeworms, hookworms, pinworms and trichomonads of dogs and cats, and the excellent broad-spectrum insect expelling effect is achieved while good palatability is kept.

The present application relates to the technical field of cell staining, and particularly relates to a vaginal secretion staining solution, a preparation method and a staining method thereof.The vaginal secretion staining solution provided by the present application uses basic fuchsin and methylene blue as staining agents, can stain epithelial cells and leukocytes, and cannot stain trichomonas and mold, so that leukocytes and trichomonas can be obviously distinguished through color difference, and personnel misjudgment caused by the similarity of leukocytes and trichomonas is reduced.The vaginal secretion staining solution provided by the present application has simple operation steps, short cycle and fast staining.The present application further provides a preparation method and a staining method of the vaginal secretion staining solution.

The application discloses an extractable nucleic acid trichomonad activity protection solution and relates to the technical field of biotechnology, which comprises two component reagents A and B, the reagent A comprises 0.8-1.0% NaCl, 0.1-5% Tris-Cl buffer, 0.1-1.5% SDS, 1.5-5% glucose, 5-15% serum, 0.5-5% betaine, and the balance is sterile deionized water, and the reagent B is a proteinase K solution. The application can not only dilute secretion, preserve trichomonas and culture trichomonas, but also be used for extracting trichomonad nucleic acid in PCR molecular experiment, so that the application can realize double application of trichomonad detection. The application not only helps to solve the health problems of women and improve fertility protection, but also is the first domestic invention to provide an example for new technology transformation service to the society.

The invention discloses a circular RNA (Ribonucleic Acid) and an application of the circular RNA in a trichomonad pigeon vaccine. The coding element contained in the circular RNA can be used for coding the recombinant trichomonas pigeon AP33 or AP65 protein. The coding element has a sequence as shown in SEQ ID NO.1 or SEQ ID NO.2 or a conservative variant sequence of the sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The circular RNA can induce protein expression in an animal body and generate a high-level antibody, does not contain infectious insect species components, can be degraded into nucleotides in the body and cannot be integrated into a host genome when being applied to a trichomonad pigeon vaccine, has no risk to pigeons in use, can stimulate the pigeons to generate relatively strong immune responses including humoral immunity and cellular immunity, and can be used for preparing a vaccine for preventing and treating the pigeons. Meanwhile, the vaccine is high in stability, can be prepared on a large scale through a molecular technology, and is low in production cost.