48 results about "Clonorchis sinensis" patented technology

The invention relates to the technical field of biology, in particular to an antigen chip used for detecting human parasites antigens in serum and a preparation method thereof. The invention spots antigen array of parasites which are seriously harmful to human body, Schistosoma, Lung fluke, Clonorchis sinensis, Cysticercosis, Sparganum mansoni, Trichina, Angiostrongylus cantonensis, Toxoplasma gondii, and the like, on a solid phase membrane carrier, and specific gamma immunoglobulin (IgG) of human parasites in serum is detected by a specially made vertical flow chip detect device, and the detecting marker is colloid gold second antibody conjugate or colloid gold protein A conjugate of fresh color. IgG antibodies in serum specially bind with the antigens on the membrane in percolation process layer upon layer, and human IgG which specially binds with the antigens is detected by the colloid gold marker. Detecting of a plurality of parasites antigen indexes in serum sample can be finished within several minutes. The device and the method have the advantages of high throughput, simple operation, convenient use and being suitable for parasitic diseases clinical test and serum-epidemiological investigation.

The invention discloses an ELISA kit, which can simultaneously diagnose the infection of Clonorchis sinensis, Paragonimus westermannii, cysticercosis and sparganosis. The kit provided by the invention can simply, quickly and accurately diagnose the infection of the main tissue parasitic helminth parasites at one time through a standardized method.

The invention discloses a PCR (Polymerase Chain Reaction) amplification kit for detecting clonorchis sinensis metacercaria on the basis of plastosome COI genes and an amplification primer. The PCR amplification kit comprises PCR amplification reaction liquid, wherein the PCR amplification reaction liquid includes 10mmol / L of Tris-HCl, 50mmol / L of KCL, 1.5mmol / L of MgCl<2>, 0.8mmol / L of dNTPs, 0.4[mu]mol / L of positive primers, 0.4[mu]mol / L of reverse primers, 20ng / [mu]L of DNA (Deoxyribonucleic Acid) templates and 0.075U / [mu]L of Taq enzyme. The PCR amplification kit has the advantages of high sensitivity, high specificity, high detection speed and high detection accuracy, and an effective technical measure is provided for diagnosis, treatment and prevention of clonorchis sinensis infection.

The invention discloses a traditional Chinese medicine preparation as well as a preparation method and an application thereof. The traditional Chinese medicine preparation is characterized in that the active ingredients include euphorbia pekinensis, euphorbia kansui and daphne genkwa. The traditional Chinese medicine preparation can effectively treat hepatitis A, effectively inhibit hepatitis B virus replication to realize the effect of eliminating hepatitis B virus, and effectively treat hepatitis B; and in addition, the traditional Chinese medicine preparation can also improve the fibrosis level of liver, effectively treat cirrhosis, effectively remove ascites from the body caused by cirrhosis, remove clonorchis sinensis, as well as effectively treat clonorchis sinensis disease.

The invention provides a clonorchis sinensis real-time fluorescence polymerase chain reaction (PCR) detection kit which comprises a PCR specific primer and a fluorescent probe, wherein the PCR specific primer consists of a clonorchis sinensis ribosome 18S upstream primer having a base sequence shown as SEQ. ID. No. 1 in a sequence list and a clonorchis sinensis ribosome 18S downstream primer having a base sequence shown as SEQ. ID. No. 2 in the sequence list; the fluorescent probe has a base sequence shown as SEQ. ID. No. 3 in the sequence list; and the 5' end of the fluorescent probe is labeled with a fluorescent reporter group and the 3' end is labeled with a fluorescent quenching group. Compared with an existing detection method, the clonorchis sinensis real-time fluorescence PCR detection method disclosed by the invention can be used for reflecting the process of target gene amplification in real time and directly showing the copy number of the target group according to internal parameters or external parameters; the detection method has the advantages of being high in sensitivity, strong in specificity, short in cycle, high in flux and the like; meanwhile, the complex operation of gel electrophoresis is avoided by detecting a fluorescence signal by virtue of an optical system; and the detection method can be used for reducing the occurrence rate of false positive and potential hazard to human bodies and environment, and the detection method can be also used for shortening detection time.

The invention discloses a primer, a probe and a kit for detecting clonorchis sinensis through a RAA fluorescence method. The kit comprises the primer, the probe, a RAA fluorescence universal reactant,a reaction buffer solution, a negative quality control substance, and a positive quality control substance. The kit carries out isothermal amplification detection at a temperature of 30 to 42 DEG C,the detection is finished within 5 to 20 minutes; the operation is simple, and the kit can be co-used with a small instrument, is portable, has the characteristics of rapidness, sensitivity, accuracy,and good repeatability, is suitable for basic detection and on-site detection, and has a good application prospect.

The invention relates to the field of biotechnology, in particular to a clonorchis sinensis recombinant protein C<s>H<sc>B and application thereof in enteritis therapeutic drug. The C<s>H<sc>B protein has an amino acid sequence shown in SEQ NO:2. According to the clonorchis sinensis recombinant protein C<s>H<sc>B and the application thereof in the enteritis therapeutic drug, through widespread and in-depth study, it is discovered that the recombinant protein C<s>H<sc>B has an obvious therapeutic effect on enteritis especially ulcerative colitis, and conditions such as body weight change, disease activity score, length of colon and inflammatory cell infiltration of animals suffering from colonitis can be obviously improved through therapy of the recombinant protein C<s>H<sc>B; and meanwhile, the clonorchis sinensis recombinant protein C<s>H<sc>B can obviously reduce the level of ulcerative colitis inflammatory factor (such as IL-6). The enteritis therapeutic drug containing the recombinant protein C<s>H<sc>B has an extremely high application prospect and value especially in ulcerative colitis field.

The invention discloses a primer group for detecting clonorchis sinensis and a detection method thereof, belonging to the technical field of parasite molecular detection. The primer group for detecting clonorchis sinensis is as shown by SEQ ID NO: 1-6. According to the detection method, a sample to be detected is amplified through loop-mediated isothermal amplification reaction by the primers, the amplification product is detected, and whether the sample contains clonorchis sinensis can be determined by adding a fluorescent chromogenic reagent and observing with the naked eye in natural light, or by detecting whether positive gradient strips exist by an electrophoresis method. The primers for detection disclosed by the invention have high sensitivity and good specificity, and the detection method has high accuracy, simplicity of operation and broad application prospects.

The invention discloses a ribosomal DNA ITS1 gene-based polymerase chain reaction (PCR) amplification kit for detecting clonorchis sinensis metacercaria and an amplification primer. The kit comprises a PCR amplification reaction solution, wherein the PCR amplification reaction solution comprises 10 mmol / L of Tris-HCl, 50 mmol / L of KCl, 1.5 mmol / L of MgCl2, 0.8 mmol / L of dNTPs, 0.4 [mu]mol / L of positive primer, 0.4 [mu]mol / L of negative primer, 20 ng / [mu]L of DNA template, and 0.075 U / [mu]L of Taq enzyme. The kit disclosed by the invention is high in sensitivity and specificity, the detection is fast in speed and accurate, and an effective technical means is provided for making a diagnosis, giving treatment and preventing clonorchis sinensis infection.

The invention relates to a method for detecting clonorchis sinensis eggs, in particular to a method for detecting clonorchis sinensis eggs in gallstone, comprising the following steps: 1) firstly grinding stones and filtering the ground stones to prepare platelets; 2) observing whether the platelets contain clonorchis sinensis eggs through an optical microscope; 3) observing the platelets or the sectioned stones through a scanning electron microscope if the clonorchis sinensis eggs are not observed; and 4) detecting the clonorchis sinensis eggs by polymerase chain reaction if the clonorchis sinensis eggs are not observed. By detecting the clonorchis sinensis eggs in gallstone, the invention obviously increases the detection rate of infection caused by clonorchis sinensis, provides a new method for clinically preventing and treating infection caused by clonorchis sinensis and exploring the correlation between the gallstone and infection caused by clonorchis sinensis so as to reduce the incidence of the gallstone, improves the precision of diagnosis and achieves the aims of early diagnosis, early treatment and early prevention.

The invention relates to the field of a biological technology, in particular to clonorchis sinensis recombination protein CsHscB (called rCsHscB for short) and an application thereof to cholestasis-induced liver fibrosis therapeutic drugs. The CsHscB protein has amino acid sequence shown as SEQ NO:2. Extensive and deep research finds that the protein has notable treatment effects on cholestasis-induced liver fibrosis. The protein treatment can obviously improve the liver function index and pathological changes of inflammatory cell infiltration, bile duct proliferation, cholestasis-induced liver fibrosis and the like of cholestasis-induced liver fibrosis animals. Besides, the recombination protein CsHscB can notably reduce the level of inflammatory factors (such as IL-6 and MCP-1 ) in cholestasis-induced liver fibrosis animal models. Therefore, the cholestasis-induced liver fibrosis therapeutic drug containing the rCsHscB protein provided by the invention has high application prospect and value.

The invention belongs to the field of resource and environment, and particularly relates to a microbiological detecting device, which mainly consists of two parts, namely, a chemical polarization field effect sensor and a detecting system. The microbiological detecting device adopts the working principle that the chemical polarization action of a to-be-detected object is mainly used for inducing the semiconductor layer as the substrate to be polarized, so as to adjust the transmission of the carriers in the semiconductor layer and consequently control the change of the output signals between a control source and a drain electrode. The detecting device can be controlled through a computer and can continuously monitor the to-be-detected object in real time. The detecting device can be used for monitoring and investigating the microbes as schistosome, plasmodium and clonorchis sinensis.

The invention discloses new genes for encoding clonorchis sinensis microsome GST-1, polypeptides encoded by the genes, and antibody of the polypeptides. The invention also discloses the depressor of the polypeptides for screening clonorchis sinensis microsome GST-1, the use of polynucleotides as primer or as probe, in particular the use of the polypeptide and polynucleotide as diagnosis reagent kit, vaccine and pharmaceutical composition.

The invention discloses a PCR (polymerase chain reaction) amplification kit for detecting metacercaria of Cs (clonorchis sinensis) on the basis of ribosomal DNA (deoxyribose nucleic acid) ITS2 (internal transcribed spacer 2) and an amplification primer. The PCR amplification kit comprises a PCR amplification reaction liquid, wherein the PCR amplification reaction liquid comprises 10 mmol / L of Tris-HCl , 50 mmol / L of KCl, 1.5 mmol / L of MgCl2, 0.8 mmol / L of dNTPs (deoxyribonucleoside-5'-triphosphate), 0.4 mu mol / L of a forward primer, 0.4 mu mol / L of a reverse primer, 20 ng / mu L of a DNA template and 0.075 U / mu L of a Taq enzyme. The PCR amplification kit is high in sensitivity and high in specificity, can detect metacercaria of Cs quickly and accurately and provides an effective technical means for diagnosis and prevention of Cs infection.

The invention relates to an anti-parasitic medicine composition and application. The composition comprises an anti-parasitic active component, wherein the anti-parasitic active component comprises left-handed praziquantel, tribendimidine and / or a tribendimidine in vivo metabolite. By mixed use of the left-handed praziquantel, the tribendimidine and / or the tribendimidine in vivo metabolite, the anti-parasitic medicine composition has the synergistic interaction effect, can effectively enhance the prevention or treatment effect and reduce the medicine use amount and the cost, and enables medicine application to be simpler. Compared with a single anti-parasitic active component medicine, the composition expands the range of an anti-parasitic spectrum, and is suitable for clonorchis sinensis and / or opisthorchis viverrini infection of human and animals. Furthermore, the composition is high in medicine compliance, high in safety and high in economy.

The invention discloses new genes for encoding clonorchis sinensis thermostable type cytoplasm malate dehydrogenase, polypeptides encoded by the genes, and antibody of the polypeptides. The invention also discloses the depressor of the polypeptides for screening the clonorchis senensis thermostable type cytoplasm malate dehydrogennase, the use of polynucleotides as primer or as probe, in particular the use of the polypeptide and polynucleotide as diagnosis reagent kit, and vaccine.

The invention discloses a clonorchis sinensis specific antibody functionalized nanogold rod. The nanogold rod is prepared by adopting a seed-mediated growth method, coating the gold nanorod with a sulfhydrylated clonorchis sinensis specific IgG antibody, and screening the clonorchis sinensis specific IgG antibody functionalized nano antibody by comparing the displacement of LSPR red peaks before and after coating. The invention further discloses the clonorchis sinensis specific antibody and application of the clonorchis sinensis specific antibody functionalized nanogold rod, and the clonorchissinensis specific antibody functionalized nanogold rod is specifically applied to detection of serum circulating antigen through the clonorchis sinensis specific antibody. According to the invention,the advantages of detection sensitivity and early stage are realized by utilizing a nanogold rod biotechnology, the operation is simple, the cost is low, and a new technical support is provided for early detection of clonorchis sinensis.

The invention relates to the technical field of biologics, and particularly relates to a primer group and a kit for detecting and identifying clonorchis sinensis and / or haplorchis taichui. The primer group is selected from a nucleotide sequence shown in SEQ ID NO.1 to SEQ ID NO.4. The primer group has the advantages of being good in specificity and high in amplification efficiency, primer dimer, non-specific amplification and cross reactions are avoided in the PCR (Polymerase Chain Reaction) amplification process, influence of non-target products to results is avoided, moreover, the amplification efficiency is high, and detection signals are intense.

The invention belongs to a medical research method, and specifically discloses a method for studying a microstructure of a clonorchis sinensis egg in gallstone. The method comprises the steps that: (1) a gallstone specimen is prepared with a conventional method, and is filmed; (2) the gallstone specimen is placed in a sample chamber of a scanning electron microscope, and is positioned under a magnification of 20-40 times; (3) a local gallstone specimen is respectively magnified to 400 times, 1000 times, 3000times, 6000 times, 10000 times and 20000 times, wherein scanning electron microscope observation and X-ray energy spectrometer analysis are carried out. According to the invention, with the scanning electron microscope and the X-ray energy spectrometer, the microstructure of the gallstone clonorchis sinensis egg is studied. According to the size and morphology of clonorchis sinensis egg and size and morphology of gallstone crystal, ordered and gradually magnified analysis is carried out, and a novel research method is provided for the discussion of clonorchis sinensis egg and gallstone formation mechanism.

The invention discloses new genes for encoding clonorchis sinensis type I aldehyde dehydrogenase, polypeptides encoded by the genes, and antibody of the polypeptides. The invention also discloses the depressor of the polypeptides for screening clonorchis sinensis type I aldehyde dehydrogenase, the use of polynucleotides as primer or as probe, in particular the use of the polypeptide and polynucleotide as diagnosis reagent kit, and vaccine.

The invention provides a detection primer, a detection kit and a detection method for clonorchis sinensis and an application, belongs to the technical field of biological assays, and aims to overcomethe defects of high cost, complicated operation, large time consumption, missed detection or false detection in existing detection methods. The invention provides a detection primer, a detection kit and a detection method for clonorchis sinensis with low cost, simple operation, short detection time and high sensitivity. According to the invention, detection can be completed within 5 min at the normal temperature, and interpretable results can be obtained. The detection kit of the present invention has 2ng / [mu]L of minimum detection concentration to clonorchis sinensis DNA and 5 times higher sensitivity than common PCR. The detection kit has 100% of specificity to clonorchis sinensis. Results can be judged visually in actual sample detection. clonorchis sinensis metacercariae and eggs can be quickly detected by adding nucleic acid dye Sybr Green I without needing PCR instruments or ultraviolet gel systems. The detection kit is suitable for quick detection in primary care or on site.

The present invention discloses one new gene coding the clonorchis sinonsis GTP bindin alopha subunit, the gene coded polypeptide and the antibody of the polypeptide. The present invention also discloses clonorchis sinonsis GTP bindin alopha subunit screening inhibitor of the polypeptide and the application of the polynucleotides as primer or probe, especially the use of the polypeptide and the polynucleotides as diagnosis kit and vaccine.

The invention discloses a PCR kit for detecting clonorchis sinensis in pigs based on a mitochondrial gene nad2. The kit comprises sterile deionized water, PCR reation liquid, Taq, DNA, polymerase, GoldView DNA dye, 6*Loading Buffer, a standard product and a reference product. The kit can quickly and accurately detect DNA of the clonorchis sinensis in pigs of a detected sample and can be applied toinvestigation of molecular epidemiology infected by the clonorchis sinensis in pigs and curative effect monitoring. Template DNA preparation is simple in steps, low in cost and simple in operation especially for large-scale detection of a large number of samples, saves time, labor, a large number of reagents and consumable materials, and improves the work efficiency. The kit can be applied to detection for the clonorchis sinensis in pigs. The application of the kit is a detection method of high sensitivity and high specificity based on the molecular biology.

The invention discloses a clonorchiasis sinensis and angiostrongyliasis cantonensis real-time fluorescence PCR (Polymerase Chain Reaction) detection reagent, and a kit and a detection method thereof, wherein the detection reagent comprises two groups of compositions taking conserved segments of clonorchiasis sinensis ITS1 gene and angiostrongyliasis cantonensis ITS2 gene as targets, and the two groups of compositions respectively comprise a pair of specific primers, a specific probe and an internal standard probe. The kit comprises PCR mixed solution, Taq DNA polymerase, DEPC-H2O, a positive quality control product, a negative quality control product and pseudovirus internal standard solution; the PCR mixed solution contains the detection reagent. The detection method comprises the steps of total DNA extraction, reaction composition preparation, amplification and result judgment. The reagent and the kit are strong in specificity, high in sensitivity, pollution-free, high-throughput, and capable of accurately detecting parasite larvae, invisible to the human eyes, in food, and the detection method is fast, specific and sensitive and capable of simultaneously detecting a great amount of samples.

The invention discloses new genes for encoding clonorchis sinensis type I aldehyde dehydrogenase, polypeptides encoded by the genes, and antibody of the polypeptides. The invention also discloses the depressor of the polypeptides for screening clonorchis sinensis type I aldehyde dehydrogenase, the use of polynucleotides as primer or as probe, in particular the use of the polypeptide and polynucleotide as diagnosis reagent kit, and vaccine.

The invention discloses a clonorchis sinensis droplet type digital PCR detection kit. The kit comprises a specific primer and a fluorescent probe, wherein the specific primer is designed according toa COI gene of a cytochrome C oxidase subunit 1 gene of clonorchis sinensis. In addition, the invention also discloses a primer and a probe for detecting clonorchis sinensis and an application of the primer and the probe in preparation of a clonorchis sinensis infection disease diagnosis product. An upstream primer of the primer is a sequence as shown in SEQ ID NO.1; the sequence of the downstreamprimer is shown as SEQ ID NO.2; and the probe has a sequence as shown in SEQ ID NO. 3. The invention successfully establishes the high-sensitivity detection kit capable of efficiently detecting clonorchis sinensis excrement DNA, and the detection kit is rapid, sensitive and specific and can be used for detecting clonorchis sinensis infection, especially mild infection.

The invention provides a specific antigen of clonorchis sinensis, and also provides a specific antibody combining the specific antigen of the clonorchis sinensis, a separated protein which is coded by a nucleotide sequence of a specific antigenic gene of the clonorchis sinensis, a vector containing the specific antigenic gene of the clonorchis sinensis, a vaccine, a kit and application of the specific antigen of the clonorchis sinensis, the antibody, the vaccine and the kit in preparing a drug for treating, diagnosing or preventing the clonorchiosis. The Cs1 antibody of the clonorchis sinensis has higher immunogenicity, can be used for easily preparing a polyclonal / monoclonal antibody, and is further applied to the aspects such as antigen detection and the like. Moreover, the stronger immune response of an experimental animal (mouse) on the antigen is realized.

The invention provides a kit for detecting porcine clonorchis sinensis. The kit consists of sterile deionized water, a fluorescent quantitative PCR (polymerase chain reaction) solution, a standard and a reference, wherein the fluorescent quantitative PCR solution contains SYBRGreenReal-timePCRMasterMix, ddH2O and detection primers. The kit has the advantages of strong specificity, high sensitivity, no pollution and high flux, and does not need electrophoresis so as to save the detection time. The kit can be used for quickly and accurately detecting the DNA of the porcine clonorchis sinensis in a detected sample, and can be used for molecular epidemiological investigation and curative effect monitoring of porcine clonorchis sinensis infection. The template DNA preparation step of the method is simple and low in cost, and the operation is simple, so that time and labor are saved, a large amount of reagents and consumables are saved, and the working efficiency is improved. The kit is applied in detection of the porcine clonorchis sinensis serving as a food-borne parasite.