65 results about "Intermediate host" patented technology

Systems and methods for computing data transmission characteristics of a network path are disclosed. In some embodiments, the network path has a sending host, at least one intermediate host, and a receiving host, and the data transmission characteristics are computed based on single-ended measurements performed at the sending host.

The invention discloses an artificial propagation method of diversinervus elegans silvestri. In the method, a pumpkin is used as propagation material, parasaissetia nigra nietner is propagated to be used as an intermediate host, the parasaissetia nigra nietner is used for producing the diversinervus elegans silvestri, the parasaissetia nigra nietner used as the intermediate host is inoculated on the pumpkin and cultured for 35-50 days under indoor natural conditions to inoculate a parasitic wasp; the proportion of the male diversinervus to the female diversinervus is controlled to be 2-4 : 1, the diversinervus are fed with 10% honey water, the proportion of the male encyrtid to the parasaissetia nigra nietner is controlled to be 1 : 5, and after 1-3 days, the pumpkin used as the propagation material is taken out; another pumpkin with the intermediate host is used as the propagation material for propagation of the diversinervus elegans silvestri, the parasaissetia nigra nietner is inoculated on the pumpkin for 1-3 days, and then, the provenances of the diversinervus are discarded after inoculating 1-2 pumpkins; the pumpkin being taken out is placed in the room for cultivation under natural conditions, the parasitized parasaissetia nigra nietner used as the intermediate host is cut off by a blade so as to collect honeybee pupae, and the grown diversinervus are collected after the eclosion of the pupae. The method can implement volume production of the diversinervus elegans silvestri, and can effectively solve the problems of low progenitive rate and delayed supply and demand of biological control.

The present invention provides methods and compositions for the construction and direct transformation of site-saturation libraries into Bacillus. This method avoids the need for the use of intermediate hosts, such as E. coli for the development of Bacillus strains suitable for the production of proteins.

A method for reproducing the Bethylidae, which can be used to prevent and elimiante the natural energy of tree (such as longican), features that the another insect "Huangfengjia" is used as its intermediate host.

The invention relates to a method for breeding a large number of microplitis bicoloratus for controlling noctuidae injurious insects, belonging to the field of insect management. Beet armyworm or prodenia litura larvas between the end of second instar and the beginning of third instar are used as intermediate hosts, and microplitis bicoloratus is used as parasitic wasps for wasp inoculation, wherein the quantity ratio of the parasitic wasps to the hosts is 1:5-10. The hosts are bred under the conditions that temperature is 25-27 DEG C, RH (Relative Humidity) is 60-70%, photoperiod L:D is 2:12 and light intensity is 500 LUX. The parasitic wasps come out of polypide for cocooning, pupation and eclosion to obtain the microplitis bicoloratus. The invention realizes the efficient breeding of a large number of high-quality graparasitic wasps, namely microplitis bicoloratus which can be applied to the control of prodenia litura; the invention provides a solid basis for applying the microplitis bicoloratus to the control of the noctuidae injurious insects in large lands. The method is not limited by seasons, ensures the long-time abundant supply source of parasitic wasps and is beneficial to the long-time efficient control of noctuidae.

The invention discloses a Japanese coccophagus expanding propagation method. The method includes the first step of host plant preparation, the second step of expanding propagation of an intermediate host, namely parasaissetia nigra, and the third step of expanding propagation and rejuvenation of Japanese coccophagus. The method is a simple and efficient intermediate host collection and inoculation method, the survival rate of the intermediate host, namely parasaissetia nigra, is increased, the utilization rate of host plants is increased to the largest extent, the parasitism rate of Japanese coccophagus on the intermediate host is increased, the number of pest populations is controlled, and the field prevention effect is ensured.

The present invention belongs to the field of biological pesticide, and is especially one kind of high efficiency and low toxicity molluscacide containing ginkgolic acid. The present invention adopts ginkgolic acid as main component, and niclosamide, copper ion and supplementary material to compound composite molluscacide for killing water snail as the unique intermediate host of blood flukes and preventing propagation of schistosomiasis. The test shows that the molluscacide has fast water snail killing effect, no damage to human body and no obvious toxicity on fresh water fish. The molluscacide may be prepared into concentrated liquid preparation or solid preparation for easy production, storing, transportation and use.

A fast spiral filter, its structure is that the filter is equipped with a main body, the front end is equipped with a feeding cylinder and the rear end is equipped with a rear end cover, the middle of the main body is provided with a filter screen skeleton and a filter screen or filter cloth is installed on it, and the upper end of the main body is provided with a pressure filter. Table, the lower end is equipped with a slope type liquid collection tank, installed with a liquid outlet hole, connected by a flange to the discharge pipe, the screw shaft is provided with a spiral piece and a material guide piece, and the feeding cylinder is installed to install the drive shaft sleeve to connect to the motor. The upper end of the cover is provided with a safety valve, the lower end is provided with a discharge hole and a control valve is installed, the rear end cover is provided with a bearing box to install bearings, and the front outrigger and the rear outrigger are installed under the liquid collection tank and the feeding cylinder. A fast spiral filter achieves the effects of continuous filtering and cake unloading, saves energy and reduces costs, has a compact structure, occupies a small area, is convenient to operate, and is safe to use to improve filtration efficiency.

The invention provides an artificial propagation method of vally sclerodermite bethylidae, comprising:(1)host cultivation,adopt the pupal of the yellow powder nail initial pupate as the intermediate host, including grub cultivation, old grub cultivation, selecting of the pupal, imago cultivation and gaining ovum; (2)rearing,include cleaning, clogging of the breeding honeybee pipe, move the seed honeybee--vally sclerodermite bethylidae into the breeding honeybee pipe;(3)graft honeybee,take the pupal of the initial pupate into the breeding honeybee pipe and breed the parasitic wasp. The natural host number does not limit the invention, it can breed a good deal of vally sclerodermite bethylidae and substantial increase the parasitic rate.

Disclosed is a recombinant live vaccine against foot-and-mouth disease virus delivered by Attenuated Salmonella choleraesuis, whose preparation consists of synthesizing the DNA sequence encoding the foot-and-mouth disease virus antigen epitope genes through chemical synthesis method, linking into complete antigen genes on the DNA horizontally to form fusion genes, inserting the fusion genes into expression plasmid vectors to construct eukaryon expression plasmid pB01, passing the eukaryon expression plasmid pB01 through intermediate host of samonella typhimurium LB5010, electrically transforming into Attenuated Salmonella choleraesuis, thus constructing the recombinant live vaccine pB01/S.cho against foot-and-mouth disease virus.

The invention belongs to the technical field of vaccine development, and provides novel echinococosis granulosis vaccine for preventing intermediate hosts of echinococosis for human, livestock and the like. The novel echinococosis granulosis vaccine comprises soluble expression mixtures of Eg95 recombinant proteins, aluminum hydroxide and CpG immune adjuvants. The novel echinococosis granulosis vaccine has the advantage that excellent cellular immunity and humoral immunity of bodies can be effectively stimulated by the novel echinococosis granulosis vaccine.

The invention discloses a preparation method of a crab breeding special disinfectant, and belongs to the technical field of aquatic disinfectant preparation. The preparation method comprises the steps: drying croton seed under the blazing sun, preparing bean meal by an oil press, then mixing with vinasse and the like, supplementing with protease and lipase, inoculating with probiotics, fermenting, then allowing tea leaves to stand for getting moldy, carrying out high pressure steaming, grinding into a slurry, and mixing with the croton seed meal fermented material, a filling powder and the like to prepare the crab breeding special disinfectant. The product prepared by fermenting the croton seed meal by the probiotics effectively kills intermediate hosts, such as oncomelania, escargots and the like, of parasites in a pond, and cooperates with the tea leaf slurry to achieve the purpose of removing animal worm eggs, spores and harmful bacteria; after the disinfectant is used, the disinfectant can be degraded by soil microorganisms, is used as nutrients absorbed by aquatic animals and plants, and effectively solves the problem of drug residues of traditional crab breeding disinfectants.

The invention belongs to the technical field of vaccine development, and provides novel echinococosis granulosis vaccine for preventing intermediate hosts of echinococosis for human, livestock and the like. The novel echinococosis granulosis vaccine comprises soluble expression mixtures of Eg95 recombinant proteins, aluminum hydroxide and immune adjuvants. The novel echinococosis granulosis vaccine has the advantage that excellent cellular immunity and humoral immunity of bodies can be effectively stimulated by the novel echinococosis granulosis vaccine.

The invention relates to a trichogramma collecting device. The collecting device comprises a bracket, a tray, a cover body, a filter net and an optical sensing device, wherein the tray is connected with the bracket and used for supporting trichogramma and intermediate host eggs; the cover body is arranged above the tray, and a gap is formed between the cover body and the tray; the filter net surrounds the outer sides of the cover body and tray and seals the gap, storage space is formed in the cover boy, the tray and the filter net, the bore diameter of the filter net makes tiny trichogramma able to enter the storage space, the storage space is internally provided with a light source, and the light source is configured to be able to give out light so as to attract trichogramma; the opticalsensing device is configured to turn on the light source when night illumination intensity is sensed and turn off the light source when day illumination intensity is sensed.

The present invention relates to a preparation method of a snail respiratory protein. A respiratory protein containing copper ions is extracted from a snail vivo used as an intermediate host of Schistosoma japonicum by virtue of mechanical extrusion, tissue dissection, hemolymph extraction, ammonium sulfate precipitation, super high-speed centrifugal separation and other means. The respiratory protein contains 0.2-0.3% of copper, the UV characteristic absorption peak is at 275nm and 340nm, and a cylindrical agreegate structure is observed under a transmission electron microscope.

An artificial reproduction method for Microplitis mediator (Haliday) with sexual dispause performance as natural enemy of pests features that the armyworm is used as intermediate host and said Microplitis mediator can be induced to form diapause source in artificial condition for storage for a long time, and the dispause may be released for application at all times according to the occurring time and quantity of harmful insects.

The invention relates to an artificial breeding method of cotesia chilonis matsumura.The method includes the steps of breeding hosts, prolonging the life of the species, breeding cotesia chilonis matsumura, delaying the propagation and storage of bred cotesia chilonis matsumura, and prolonging the life of host cotesia chilonis matsumura with Evergestis extimalis Scopoli larvae three to four years old as the intermediate hosts by means of external nutrients, thereby increasing the host search frequency of male and female cotesia chilonis matsumura.In addition, by means of the diapause of female cotesia chilonis matsumura, cotesia chilonis matsumura can be stored for a long time in the set environment; by means of lots of exploration, diapause is removed at any time for application under proper conditions.Materials for the method are easy to obtain, the breeding rate is high, stability is strong, and the problems that in an artificial transgenerational breeding method, the bee species is short in life and bees can hardly meet demands are solved.

The invention provides applications of a TGF-beta1 receptor blocker in preparation of medicines treating the Hydatid disease. Concretely, through immune escape of TGF-beta1 receptor blockers for inhibition of infection of intermediate hosts by echinococcosis granulosis cysts, increase of the number of CD<4+>T cells, decrease of the number of CD<8+> T cells, raising of the CD<4+>/CD<8+>T cell ratio, decrease of the number of CD<4+>CD<25+> T cells, increase of expression of active receptors NKG2D of NK cells and raising of the cracking rate of target cells Yac-1 by NK cells in host cells are caused. Researches show that NK cell viability is in positive correlation with active receptor NKG2D number. Results show that the provided method can enhance inhibition of hosts to echinococcosis granulosis cyst infection effectively. The method can be used for treating the Hydatid disease effectively, and has good clinic application prospects.

The invention provides a prevention method for river crab diseases, which achieves the goals of remarkable prevention effects on the diseases of cultivated river crabs and yield increase and income increase of fishery. The prevention method for the river crab diseases comprises treating a crab pool during a cultivation preparation period of the river crabs. The prevention method is characterized in that after water is injected into the crab pool, chemicals are used according to a certain concentration to perform insect killing and sterilization to the crab pool so as to kill pathogens of the river crab diseases and various shellfish which are major intermediate hosts of the pathogens, and after a certain time, quicklime is mixed with the water according to a certain dosage and sprayed to the whole pool. The prevention method is favorable for reducing incidence of the river crab diseases, and is particularly favorable for reducing incidence of a trembling disease of the river crabs, thereby being favorable for increasing cultivation yield and economic benefit of the river crabs.

The invention relates to a comprehensive pest preventing and controlling method for tea planting. The method includes the steps of planting osmanthus trees between tea trees to achieve shade and prevent pests, using oil cake and farmyard manure to fertilize the tea trees, timely picking tea leaves, and controlling occurrence of empoasca flavescens, leaf mites, and leaf bud diseases; turning over soil of the tea trees in late autumn, preventing and controlling wintering lepidopteran pests in the soil, pruning tree branches, cultivating good tree crowns, enhancing the growth of the tea trees, changing the growth environment of the pests and pathogens, cutting off tree branches with the pests and the diseases and fine and close tree branches, cleaning and deeply burying dead branches and falling leaves, and preventing and controlling leaf and wintering pests; at the same time, installing pest control lamps and pest sticky boards in a tea garden to attract pests, and timely cleaning weedsin the tea garden, so that the pests and the pathogens loose intermediate hosts. The method is particularly suitable for prevention and control of diseases and pests of small leaf tea in Yanjin County, Yunnan Province.

The invention discloses a pyemotidae production method, which solves the problems of difficult batch production of substitute hosts and the unmatched obtaining time and usage time in the existing natural enemy pyemotidae production. The pyemotidae production method adopts the following steps of: a, selecting the substitute hosts to unstick, wherein the substitute hosts are roe; b, refrigerating after laminating; c, selecting the intermediate hosts to proliferate the pyemotidae, wherein the intermediate hosts are the barley pest beginning hatchpupa; and d, inoculating the substitute hosts to cultivate. Through selecting, treating the substitute hosts and improving the inoculation and cultivation method and device, the problems of difficult batch production of the substitute hosts and the unmatched obtaining time and usage time in the existing natural enemy pyemotidae production are overcome.

The invention discloses a trichogramma laid egg collecting device which is used for facilitating uniform distribution of trichogramma and making full use of intermediate host eggs for parasitism. Thedevice comprises a box, a partition plate frame, a plurality of partition plates and egg laying plates, a dark box is arranged at the left end of the box, the right end of the box is transparent, thepartition plate frame is erected in the box, the partition plates are movably arranged on the partition plate frame, the partition plates are bilaterally swung front and back relative to the partitionplate frame, the partition plates and the partition plate frame can be relatively static, the egg laying plates are arranged on the partition plates and coated with the intermediate host eggs. According to the device, after the intermediate host eggs are placed, the trichogramma lays eggs on the egg laying plates, the egg laying plates can be obliquely arranged according to habits of the trichogramma, so that the trichogramma moves towards high positions, strong and weak illumination is arranged, so that trichogramma is driven to move towards one side of the strong illumination, and the trichogramma is driven to move.

The invention relates to the technical field of biological medicine engineering and discloses a preparation method of recombinant lactobacillus plantarum expressing a porcine circovirus type-3 (PCV3)Cap gene. The method comprises the following step of S1, design and synthesis of primers, wherein the primers LpS-F and LpS-R for amplifying the sequence of the PCV3 Cap gene are designed through Primer 5.0 software according to a PCV3 gene sequence (login number: AYM55798.1) in GenBank, and the fragment length is 789bp. According to the preparation method of recombinant lactobacillus plantarum expressing the porcine circovirus type-3 Cap gene, lactobacillus plantarum is selected to express the PCV3 Cap gene which contains the signal peptide 1320 and is optimized by DCpep, lactococcus NZ3900 is selected as a cloning intermediate host, and a high content of recombinant plasmids are obtained; then, lactobacillus plantarum Lp12 is used for expressing the PCV3 Cap protein, an effective preparation and identification method is established, a detection means is optimized, and a theoretical foundation is laid for developing type-3 porcine circovirus candidate vaccines taken orally. By means of the preparation method, recombinant lactobacillus plantarum for expressing the PCV3 Cap protein is created, and the feasibility of expressing heterologous proteins through lactobacillus plantarum isenriched.