10 results about "Schistosoma" patented technology

Disclosed are Schistosoma japonicum eggs and the ingredients of secretions and excretions thereof, comprising eggs, egg culture supernatant, and egg secreted proteins. (hereinafter referred to as “Schistosoma eggs and secretions and excretions thereof” for short). A Schistosoma infection can significantly inhibit metastatic tumors of organs such as lungs, and an anti-tumor effect is mediated by the “Schistosoma eggs and secretions and excretions thereof”, which have the effect of inhibiting various host tumors, comprising lung cancer, liver cancer, melanoma, and hematological tumors. Two egg secreted proteins having an anti-tumor effect are further identified. The “Schistosoma eggs and secretions and excretions thereof” exert an anti-tumor effect by activating natural immunity such as alveolar macrophages. The “Schistosoma eggs and secretions and excretions thereof” have a potential application value in prevention and treatment of tumors in humans.

The application discloses double-stranded RNA for killing schistosoma and application thereof, wherein the nucleotide sequence of the positive strand of the double-stranded RNA is shown as SEQ ID NO:1. Through animal experiments, the double-stranded RNA has good killing effect on the early infection establishment stage (1-30 dpi) of the worm body, the worm development stage (18-30 dpi) after infection establishment, and the mature worm stage (26-42 dpi) after infection establishment, can not only prevent schistosoma from establishing infection in the host body and effectively inhibit the growth and development of the worm body, but also can reduce the oviposition of female worms, even kill the worm body with established infection, and reduce the pathological damage to the host, has great treatment potential, and can be used for treating schistosoma infection at different development stages.

The application discloses an ecological regulation model for indoor breeding of oncomelania, and relates to the technical field of snail breeding regulation. The model comprises a matching relationship between the number of indoor breeding units and the total amount of oncomelania breeding, which is used for realizing the standardized control of the initial oncomelania quantity of each breeding unit, ensuring the initial ecological balance of the experimental system, and determining the environmental regulation priority of the indoor oncomelania breeding unit based on the matching relationship. The application dynamically corrects the weight of the environmental parameter based on the risk intensity of different transmission stages of schistosoma, breaks through the bottleneck that the static prevention and control strategy is difficult to adapt to the change of the life cycle of parasites, and further realizes real-time monitoring of the fluctuation of biological characteristic parameters in the mating period, the pregnancy period and the reproductive period, so that the environmental regulation can accurately respond to the biological rhythm of the oncomelania population, ensures the sustainability of the indoor breeding of oncomelania to a certain extent, and reduces the risk of schistosomiasis transmission.

The utility model belongs to the field of cercaria escape, particularly relates to a schistosome cercaria escape device, and aims to solve the problems that when oncomelania escape is carried out in the prior art, flowing of water flow in a real environment cannot be well simulated, an angle-adjustable illumination system cannot be arranged, the cercaria escape effect is poor, experimental equipment cannot be sterilized after an experiment is finished, and the working efficiency is low. In order to solve the problem that cercaria possibly remains in a box body and causes pollution, the utility model provides the following scheme: the device comprises a box body, supporting legs are fixedly connected to four corners of the bottom of the box body, a controller is arranged on the box body, a heater and a temperature sensor are arranged in the box body, and one side of the box body is fixedly communicated with a water inlet pipe; the top of the box body is open, a fixing plate is fixedly connected to the interior of the box body through bolts, natural water flow and illumination in different time periods can be well simulated through the disturbance mechanism and the adjusting mechanism, and the cercaria releasing process is accelerated.

The invention provides a primer group, a kit and a method for detecting MNP marker sites of schistosoma and oncomelania in a water environment and application of the primer group, the kit and the method. The primer group comprises at least one of a first primer pair to a fourteenth primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the fourteenth primer pair and the reverse primer of the fourteenth primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 28 in a sequence table. The primer group has amplification compatibility, amplification can be carried out in a reaction system by adopting a super-multiplex PCR technology, amplification of 14 MNP marker sites in the reaction system is realized, sequence analysis of amplification products is carried out by fusing a next-generation sequencing platform, and identification of schistosoma japonicum and oncomelania hupehensis by one reaction is realized. The method has a comprehensive, efficient, sensitive and accurate detection effect.

The invention discloses application of 5HTR1 and Sj5HTR1 gene segments, dsRNA for interfering schistosome and application of the dsRNA, the nucleotide sequence of a sense strand of the dsRNA is as shown in SEQ ID NO.1, the nucleotide sequence of an antisense strand of the dsRNA is as shown in SEQ ID NO.2, and the dsRNA can be applied to preparation of anti-schistosomiasis drugs. The dsRNA is targeted to a 5-hydroxytryptamine receptor 1, development and survival of schistosome and oviposition of female schistosome can be remarkably inhibited, damage of schistosome to host pathology can be effectively reduced, and it is also shown that Sj5HTR1 is a potential anti-schistosome treatment target and plays an important role in schistosome biology.

The present invention relates in particular to an in vitro method for detecting parasite DNA of the genus Schistosoma from a urine sample of a subject likely to have been infected by said parasite, comprising an isothermal amplification step with a set of pan-specific primers capable of simultaneously amplifying and detecting the species S. haematobium, S. mansoni, S. bovis, S. curassoni, and S. japonicum. It also relates to a simplified in vitro method, adapted to endemic areas, for extracting parasite DNA, particularly of the genus Schistosoma, from a biological sample (e.g., urine) of a subject likely to have been infected by said parasite, comprising steps of DNA concentration by decantation, lysis with heating but without agitation or centrifugation, and filtration and elution of the parasite DNA.

A compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use in the treatment of a patient infected with pathogenic organisms; wherein X and Y are each independently selected from C, N, O and S, provided at least one of X and Y is N, O or S; wherein R1 and R2 are each independently selected from an optionally substituted C1-C8 alkyl, C1-C8 alkenyl, aryl, heteroaryl or alkylaryl group; and wherein Z is selected from H, —CN, —NO2, —NR3R4, —NR5(CO)R6; C1-C4 alkyl, C1-C4 alkoxy, —(CO)WR12, hydroxy, amino, thiol, chloro, fluoro, CF3, CHF2 or CH2F groups. The compounds may be effective in treating patients / animals infected with parasites selected from Schistosoma, Haemonchus, Eimeria, Echinococcus, Dirofilaria, Fasciola or Plasmodium parasites. Additionally or alternatively, the compounds of formula (I) may be effective in treating patients infected with pathogenic bacteria selected from S. aureus, MRSA and Enterococcus faecalis.

The present invention provides methods for detecting an anti-Schistosoma antibody in a sample obtained from a subject. Corresponding compounds, compositions, kits and methods for monitoring a subject to determine if they have contracted a Schistosoma infection are also provided.

The invention belongs to the field of biotechnology modeling, and discloses a method for establishing a schistosoma sinensis induced mouse hepatic fibrosis model. The method specifically comprises a preferable method for breeding schistosoma sinensis infectious oncomelania in a laboratory, wherein the preferable number of cercaria infected by each mouse through abdominal skin inoculation is 40 + / -2, and the cercaria comes from more than or equal to 4 positive oncomelania. The pathological change prompts that the mouse liver of the model group progresses from worm egg granuloma to hepatic fibrosis, and finally the schistosoma cirrhosis is caused. And four continuous animal experiment observations (38 weeks for the first time, 57 weeks for the second time, 32 weeks for the third time and 21 weeks for the fourth time) prove that the method has the advantages of high modeling success rate, low mouse death rate, long-term observation, easiness in popularization and the like. The schistosoma sinensis induced mouse hepatic fibrosis model is established for the first time and is not reported before. In addition to the schistosoma japonicum katsurada, the invention provides a novel schistosoma japonicum katsurada hepatic fibrosis animal model which has extremely high application value.