Primer group, probe and kit for detection of Schistosoma mansoni

A technology of Schistosoma mansoni and a kit, which is applied in the field of primers, probes and kits for detecting Schistosoma mansoni, which can solve the problems of high cost, high false positive, cumbersome and difficult pathogen isolation and cultivation, etc., and achieve simple operation and specificity Strong, sensitive and accurate results
CN110791576APending Publication Date: 2020-02-14JIANGSU INST OF PARASITIC DISEASES +1

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU INST OF PARASITIC DISEASES
Publication Date
2020-02-14

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Abstract

The invention provides a primer group, probe and kit for detection of Schistosoma mansoni. The primer group includes an upstream primer and a downstream primer, wherein the sequence of the upstream primer is shown in SEQ ID No. 1, the sequence of the downstream primer is shown in SEQ ID No. 2. The primer group for detection of Schistosoma mansoni and a probe and kit for detection of Schistosoma mansoniare has simple and convenient operation. It is verified through tests that when the primer group, probe and kit for detection of Schistosoma mansoni are applied to detection of Schistosoma mansoni, high specificity, high sensitivity, high accuracy and good repeatability are achieved, test results can be obtained within 20 minutes, and the primer group, probe and kit have low requirements fortest conditions and technologies of the test staff and low test cost.
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Description

technical field

[0001] The invention relates to the technical field of molecular biology detection, in particular to a primer set, a probe and a kit for detecting schistosoma mansoni. Background technique

[0002] Schistosomiasis is a global parasitic disease that seriously endangers human health. There are five main types of human schistosomiasis, namely Japanese, Egyptian, mansoni, intercalated and Mekong schistosomiasis, among which the infections caused by Egyptian (Schistosoma haematobium), Mansoni (S.mansoni) and Japanese schistosomiasis (S.japonicum) Schistosomiasis is the most prevalent. Among them, schistosomiasis mansoni is the most widely distributed and has the largest number of patients. It is distributed in 54 countries in the Arabian Peninsula, Africa and Latin America, and the number of people at risk is about 400-500 million.

[0003] As the number of people going abroad for labor, construction, business and tourism in my country has increased year by year...

Claims

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