77 results about "Parasitic disease" patented technology

The invention discloses a toxoplasma gondii attenuated vaccine strain RH delta rop67 as well as a construction method and application thereof, and belongs to the technical field of parasitic disease prevention and control and biological product preparation. The attenuated vaccine strain is constructed by performing targeted knockout on the ROP67 gene in a toxoplasma gondii strain RH delta ku80 through a CRISPR / Cas9 mediated gene editing technology. Compared with a wild type strain, the attenuated vaccine strain shows remarkable attenuation characteristic and good immunogenicity. A test result shows that the attenuated vaccine strain can induce a host to generate specific immune response mainly based on cellular immunity, maintains a protection effect on toxoplasma gondii infection in a relatively long immune period, and has a protection effect on tachyzoite infection and a chronic infection stage of toxoplasma gondii strains with different virulence; the survival ability of a host to tachyzoite infection can be improved, and the formation level of cysts in tissues is reduced. The invention provides a technical scheme with long-term immune potential for research and development of toxoplasma gondii attenuated vaccines.

The application discloses a kind of carrier, kit and application for long-acting gene knockdown of animal parasitic nematode, belong to the field of animal parasitic disease prevention and control.The lentivirus vector in the application integrates specific coding sequence to the genome of the infected nematode, stably and continuously expresses short hairpin RNA (shRNA) in the nematode, efficiently produces primary single-stranded small RNA (siRNA), these siRNAs target homologous target gene mRNA to cause its degradation, realize stable and continuous gene silencing effect.The application breaks through the bottleneck of traditional animal parasitic nematode RNA interference (RNAi) technology with low efficiency and instability, significantly improves the timeliness of conventional RNAi, can be used to establish long-acting RNAi technology system of animal parasitic nematode, has application value in the research of host in vivo process such as nematode infection, parasitism and pathogenicity and animal parasitic nematode disease prevention and control.

The invention provides application of naphthoquinone compounds in preparation of insecticides for preventing and / or treating parasitic diseases in aquatic animals, and belongs to the technical field of aquaculture. The naphthoquinone compound disclosed by the invention is 2-chloro-1, 4-naphthoquinone, and can be used for killing or preventing pyramidal bodies in aquatic animals. The invention also provides an insecticide for killing or preventing pyramidal worms in aquatic animals by using the naphthoquinone compound, and the insecticide comprises the following raw materials in percentage by weight: 20-30% of the naphthoquinone compound, 5-10% of a surfactant, 5-10% of a dissolving agent and the balance of water. An in-vitro insecticidal experiment and an in-vivo insecticidal experiment prove that the 2-chloro-1, 4-naphthoquinone can be used for efficiently killing insects; and the 2-chloro-1, 4-naphthoquinone has no obvious toxic or side effect on the fish body, and does not influence the growth and physiological functions of the fish body.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

The present disclosure provides novel chemotypes, pharmaceutical compositions thereof, and method of use thereof for treating parasitic infection, including infection caused by parasitic flatworms.

The invention relates to the technical field of medicinal chemistry and parasitic disease prevention and treatment, and particularly discloses a novel harmine derivative, a synthesis method and application of the novel harmine derivative to toxoplasma gondii disease resistance. The structural general formula of the derivative is shown in the specification. Wherein R1 is selected from one of hydrogen, C1-4 alkyl, substituted or non-substituted five-membered aryl or six-membered aryl, and substituted or non-substituted five-membered heteroaryl or six-membered heteroaryl containing 1 to 4 heteroatoms selected from N, O or S; r9 is selected from one of hydrogen, substituted or unsubstituted five-membered aryl or six-membered aryl; and R7 is selected from hydrogen, C1-4 alkyl, substituted or unsubstituted five-membered aryl or six-membered aryl. Preferably, the compound is 1-methyl-9-(3-methylpyridine)-beta-carboline-7-ol, and the formula is shown in the description. The novel harmine derivative provided by the invention shows high-efficiency and low-toxicity anti-toxoplasma gondii activity, the synthesis method is simple, and an excellent candidate compound is provided for developing novel anti-toxoplasma gondii drugs.

The application discloses a method for rapidly culturing trichodinid, and belongs to the field of aquatic biological technology. Trichodinid widely parasitizes on the body surface of many marine and freshwater economic fishes, is one of main parasitic pathogenic agents of the cultured fishes, is easily transmitted by indirect contact between hosts, causes large-scale death of fry, and brings huge economic losses to fish culture industry. However, trichodinid cannot be cultured in vitro, and its natural infection has seasonal regularity, so that the acquisition of experimental materials of trichodinid has great limitations, the research on trichodinid has been less, and the development of trichodinid disease prevention and treatment drugs and vaccines is hindered. The application achieves the effect that the trichodinid parasitizing on the body surface of the young fish with a certain initial trichodinid infection amount is massively reproduced in a short time by injecting an immunosuppressive agent into the young fish. The method is simple in operation, can significantly shorten the experimental period of trichodinid related research, and is beneficial to the development of drugs and vaccines for preventing and treating trichodinid disease.

The present invention. relates to a disinfectant composition against parasitic organisms. The invention also relates to a method for disinfecting at least one area to prevent parasitosis. The invention also relates to the use of at least one compound selected from fatty acids and fatty alcohols and which have a chain length of to carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms, in particular eggs or oocysts, of parasitic organisms.

Subject matter of the present invention is an angiotensin-receptor-agonist and / or a precursor thereof for use in the treatment of a disease in a subject, • wherein said disease is selected from the group comprising heart failure, chronic heart failure, acute heart failure (AHF), myocardial infarction (MI), stroke, liver failure, burn injuries, traumatic injuries, severe infection (microbial, viral (e.g. AIDS), parasitic diseases (e.g. Malaria)), SIRS or sepsis, cancer, acute kidney injury (AKI), CNS disorders (e.g. seizures, neurodegenerative diseases), autoimmune diseases, vascular diseases, hypotension and shock, and • wherein said subject has an amount of DPP3 protein and / or DPP3 activity in a sample of bodily fluid that is above a predetermined threshold.

The invention provides application of gossypol in preparation of chicken feed or medicine for preventing and treating chicken coccidiosis, and relates to the technical field of parasitic disease prevention and treatment. Experimental exploration proves that gossypol has a new application of preventing and treating chicken coccidiosis, a new scheme is provided for development of drugs and feeds for preventing and treating chicken coccidiosis, a new idea is provided for clinical control of chicken coccidiosis, and the application range of gossypol is expanded. Gossypol acts on chicks infected with chicken eimeria tenella disease, compared with chicks in a challenge control group, the reduction rate of oocyst discharge of chicks in a gossypol treatment group reaches 82.5%, the relative weight gain rate can reach 90.8%, the coccidiosis resistance index of the chicks can reach 167.4, and bleeding symptoms caused by chicken coccidiosis can be effectively reduced.

In some aspects, a method of regulating oxidoreductase activity for treating an inflammation or age-related disorder involves administering to an individual isomyosmine or a pharmaceutically acceptable salt thereof. In other aspects, a method of treating oxidative stress associated with an inflammation or age-related disorder involves administering to an individual a therapeutically effective amount of isomyosmine or a pharmaceutically acceptable salt thereof. In other aspects, isomyosmine or a pharmaceutically acceptable salt thereof may be administered to an individual for the treatment of infectious or parasitic diseases or various other disorders.

The present invention relates to a disinfectant composition against parasitic organisms. The invention also relates to a method for disinfecting at least one area to prevent parasitosis. The invention also relates to the use of at least one compound selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms, in particular eggs or oocysts, of parasitic organisms.

The invention belongs to the technical field of veterinary drugs, and particularly relates to eugenol derivatives and application thereof in preventing and treating parasitic diseases of animals, the eugenol derivatives have remarkably improved acaricidal activity, show an excellent killing effect on various livestock and poultry mites, have low toxicity, do not have obvious harm to livestock and poultry and the environment, and can be applied to prevention and treatment of parasitic diseases of animals. The requirements of green veterinary drug development are met, and high stability is shown under the environmental conditions of illumination, temperature change and the like; the preparation method of the eugenol derivative is green and environment-friendly, is simple and convenient to operate, can be used for large-scale preparation, and has relatively high industrial application potential.

This invention discloses a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae, belonging to the field of biotechnology. This invention discovers that compound vitamins and / or bovine liver extract powder as auxiliary components can promote the development of early fourth-stage larvae of Haemonchus contortus into late fourth-stage larvae and adults. This invention provides a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae. The culture medium includes a basal culture medium and auxiliary components, including compound vitamins, bovine liver extract powder, pyruvate, non-essential amino acids, L-glutamine, fetal bovine serum, defibrinated sheep blood, and L-glutathione. The cultivation method includes the step of adding early fourth-stage larvae to the culture medium for cultivation. This invention enables the in vitro culture of adult Haemonchus contortus, which is beneficial for promoting the development of related drugs and vaccines for this parasite and has significant implications for the prevention and control of parasitic diseases.

This invention relates to the application of NR3C1 targeting in the treatment of liver fibrosis. Specifically, this invention provides the use of an NR3C1 agonist for preparing a composition or formulation for the prevention and / or treatment of liver fibrosis. The composition or formulation of this invention is effective in treating liver fibrosis caused by parasitic diseases.

The invention discloses an echinococcus granulosus PLGA-EgG1Y162 nano subunit vaccine and a preparation method thereof, and relates to the field of biomedical engineering and parasitic disease prevention and treatment. According to the method, echinococcus granulosus EgG1Y162 recombinant protein (the amino acid sequence is as shown in SEQ ID NO.1) is taken as an antigen core, and is stably coated in a polylactic acid-glycolic acid copolymer (PLGA) nano carrier through an innovative embedding process, so that the nano subunit vaccine in a brand new form is constructed. Compared with pure EgG1Y162 recombinant protein, the nanoparticles prepared by the preparation method disclosed by the invention not only have innovated components, but also obviously improve the slow release performance of drugs, and can realize precise enrichment and long-acting release of antigens. The invention fills up the blank of research on the echinococcus granulosus PLGA nano subunit vaccine, provides a novel technical scheme for prevention and treatment of echinococcosis, and has important application value and market prospect in the field of biological medicine.

Provided herein is a method of treating, preventing, or alleviating one or more symptoms of Chagas disease in a subject, comprising administering to the subject: (i) ascorbic acid, or a single enantiomer, a mixture of enantiomers, or a mixture of diastereomers thereof; or a pharmaceutically acceptable salt, solvate, or hydrate thereof; (ii) a quinone compound, or a single enantiomer, a mixture of enantiomers, or a mixture of diastereomers thereof, or a pharmaceutically acceptable salt, solvate, hydrate, or prodrug thereof; and (iii) an antiparasitic agent.

The invention relates to a manufacturing process of a layered microbial fermentation bed for livestock and poultry and the fermentation bed, and belongs to the field of breeding. Oxygen supply is guaranteed by optimizing a layered structure, rapid and thorough aerobic decomposition of excrement is realized by utilizing efficient composite flora, and the generation source of malodorous gases such as ammonia gas and hydrogen sulfide is basically eliminated. The humidity, harmful gas concentration and dust in the house are remarkably reduced, and a drier, cleaner and more comfortable living environment is provided. Animal health is improved, ammonia stimulation is reduced, and respiratory diseases are reduced; pathogenic bacteria are reduced, digestive tract diseases are reduced, the incidence rate of skin diseases and parasitic diseases is reduced, and the medication cost is reduced.

Compounds of formula (I) and pharmaceutically acceptable salts thereof, useful as treatments for parasitic diseases are provided. Also provided are pharmaceutical compositions comprising the compounds or pharmaceutically acceptable salts and methods of using the compounds, pharmaceutically acceptable salts, and pharmaceutical compositions in the treatment of various disorders, including parasitic disease or infection.

The invention belongs to the technical field of in-vitro molecular detection of pathogenic microorganisms, and particularly relates to a primer probe combination, a kit and a detection system for detecting entamoeba histolytica, giardia lamblia and cryptosporidium and application of the primer probe combination, the kit and the detection system. The primer and probe combination is obtained by optimizing primer and probe design and comprises primers and probes for detecting entamoeba histolytica, giardia blue and cryptosporidium, three types of parasite pathogens can be identified and detected on suspected infected patients at a time, the flux is high, the detection time is short, the detection time is short, and the detection efficiency is high. And the kit has the advantages of strong specificity, high sensitivity, good stability, short reaction time and the like. Furthermore, the kit containing the primer and probe combination is formed by optimizing a reaction system, a single tube in the kit can contain all primers, probes and components required by PCR reaction in a fully premixed form, the reaction system does not need to be prepared, the operation is simple, and the consumed time is short.

To provide a therapy guidance and / or therapy monitoring for treatment with an angiotensin-receptor-agonist and / or a precursor thereof.SOLUTION: Subject matter of the present invention is an angiotensin-receptor-agonist and / or a precursor thereof for use in the treatment of a disease in a subject, wherein the disease is selected from the group consisting of heart failure, chronic heart failure, acute heart failure (AHF), myocardial infarction (MI), stroke, liver failure, burn injuries, traumatic injuries, severe infection (microbial, viral (e.g. AIDS), parasitic diseases (e.g. Malaria)), SIRS or sepsis, cancer, acute kidney injury (AKI), CNS disorders (e.g. seizures, neurodegenerative diseases), autoimmune diseases, vascular diseases, hypotension and shock, and wherein the subject has an amount of DPP3 protein and / or DPP3 activity in a sample of bodily fluid that is above a predetermined threshold.SELECTED DRAWING: None

This invention provides the application of Usnea powder, Usnea acid, or sodium usneate (or Usnea powder, Usnea acid, or sodium usneate as the sole active ingredient) in the preparation of broad-spectrum antiparasitic drugs for aquatic animals, drugs for treating complications caused by parasitic infections, feed additives, or functional feeds. By utilizing Usnea, Usnea extract, Usnea acid, and Usnea salts, various parasites, including ciliates, worms, and protozoa, that infest the surface and interior of aquatic animals during aquaculture can be eliminated or killed, thus maintaining the health of farmed animals, improving the success rate of aquaculture, achieving broad-spectrum and highly effective prevention and control of aquatic animal parasitic diseases, reducing the use of chemical drugs, and providing good aquaculture, ecological, and social benefits. It is safe, broad-spectrum, and highly effective, facilitating large-scale promotion.

The utility model provides a fish parasite detection kit which comprises a kit body and a kit cover, the kit body is hinged with the kit cover, a first positioning plate and a second positioning plate are arranged in the kit body, the second positioning plate is located below the first positioning plate, and the first positioning plate and the second positioning plate are both provided with positioning holes for test tubes to be inserted in. A clamping mechanism is arranged at the position, corresponding to the positioning hole, of the first positioning plate and is used for clamping the test tube. Due to the arrangement of the first positioning plate and the second positioning plate, when the test tube is sequentially inserted into the positioning holes in the first positioning plate and the second positioning plate, the test tube can be effectively prevented from inclining laterally, then the test tube is clamped through the clamping mechanism, and the stability of the test tube in the detection process is further improved.

The present invention provides new storable and stable disinfectant compositions comprising use of a solid precursor of an oxidized state of chlorine. The component in the device is devoid of stability issues, since the solid precursors are instantly to be dissolved in a pharmaceutically-acceptable diluent, adjuvant, or carrier, and combined with an activator (e.g. acetic acid or its salts), optionally combined with a viscosity enhancer, optionally combined with a dye. The main product is generated by breaking or opening seals, barriers or ports between an optional number of compartments in a multi-compartment device to mix the contents in the compartments, followed by gently squeezing or shaking the device to generate the disinfectant solution, wherein the resulting solutions can be taken out through a cap, port or opening on the multi-compartment device. The resulting compositions are useful disinfectants for treating a broad spectrum of pathogenic bacterial and / or viral, fungal or parasitic pathogens, denoted microbials.

This invention relates to the field of fecal testing, and more specifically, to a method for detecting human parasitic pathogens without the use of slides and coverslips, comprising the following steps: Step 1, adding an appropriate amount of water to the culture wells of a cell culture plate, placing an appropriate amount of feces in the water of the culture well and stirring evenly; Step 2, cutting a piece of textile or nylon mesh of similar size to the culture well and placing it into the culture well to isolate the fecal residue and fecal liquid from each other; Step 3, after stirring and mixing the fecal liquid, transferring the fecal liquid to the culture wells of a new cell culture plate, covering it with the cell plate cover, and examining it under a microscope using an inverted microscope.

The invention discloses a culture medium and a culture method for promoting development of haemonchus contortus larvae, and belongs to the technical field of biology. The compound vitamin and / or the bovine liver extract powder as the auxiliary component can promote the development of the four-stage early larvae of the haemonchus contortus to the four-stage final larvae and adults of the haemonchus contortus. The invention provides a culture medium and a culture method for promoting development of haemonchus contortus larvae, the culture medium comprises a basic culture medium and auxiliary components, the auxiliary components comprise composite vitamins, bovine liver extract powder, pyruvic acid, non-essential amino acids, L-glutamine, fetal calf serum, defibrin sheep blood and L-glutathione; the culture method comprises the step of adding fourth-stage early larvae into the culture medium for culture. According to the method, in-vitro culture of haemonchus contortus adults is achieved, research and development of related drugs and vaccines of parasites are promoted, and the method has important significance on prevention and control of parasitic diseases.

FIELD: veterinary science.SUBSTANCE: used to treat dermatitis of parasitic etiology in carnivores. The medications selamectin and fipronil are applied to the skin between the shoulder blades at the base of the animal’s neck. After which they are treated with a solution of ethyl alcohol. Next, using a sprayer, the surface is treated with electrochemically activated water with an acidic fraction with a pH of 2.0 in the amount of 20 ml per 15–20 cm2 for 15–18 s from a distance of 15–18 cm, then held for 40 min, after which the surface is treated with electrochemically activated water with an alkaline fraction with a pH of 9.0 in the amount of 20 ml per 10–12 cm2 for 15–18 s from a distance of 15–18 cm. The treatment is carried out twice a day until the skin heals.EFFECT: method provides for combined highly effective eradication of ectoparasites and suppression of pathogenic microflora with simultaneous acceleration of regeneration of damaged tissues due to the synergistic action of systemic-contact insectoacaricides and sequential local treatment of affected areas of the skin with electrochemically activated solutions with regulated acid-base balance.1 cl, 1 dwg

The application discloses a method for preventing and treating Hypentoma abdominalis and other parasitic diseases, which comprises the following steps: on the first day, feeding vitamin C bait after formalin immersion, and then adding bacillus subtilis in the breeding tank; on the second day, feeding vitamin C bait after formalin immersion; on the third day, feeding aquatic multi-vitamin bait after formalin immersion, and adding vitamin C in the breeding tank; on the fourth day, first adding oxytetracycline hydrochloride in the breeding tank after formalin immersion, then adding vitamin C, feeding aquatic multi-vitamin bait after vitamin C immersion, and finally adding bacillus subtilis in the breeding tank; on the fifth day, feeding aquatic multi-vitamin bait after vitamin C immersion in the breeding tank, and then immersing. The method has the advantages of short time effect, fast effect, simple operation, convenience, and the like, can effectively cure Hypentoma abdominalis and other parasitic diseases, can prevent secondary infection of molds and bacteria, can reduce stress of the seahorse, can improve immunity, can significantly reduce the mortality of Hypentoma abdominalis, and can improve the economic benefits of breeding.