468 results about "Breed" patented technology

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300124, a KASP type molecular marker 300124 closely linked with the cowpea salt tolerance major QTL is located at the position of 43716091 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork tenderness. An SNP site exists at the 232bp position from the 5'end of the sequence, the polymorphism is G / A, three genotypes of GG, GA and AA are shown, G allele is dominant allele, and the meat tenderness of a GG type individual is obviously higher than that of an AA type individual. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding or pork tenderness identification, and a method for identifying the pork tenderness is developed, which is beneficial to accelerating genetic breeding and early-stage seed selection of boars with high-quality meat traits.

The invention discloses a flexible self-adaptive saline goose production method which comprises the following steps: firstly, obtaining a goose blank; obtaining weight and variety information of the goose blank through an information acquisition unit I; calling standard process parameters corresponding to varieties and weights in a database through a control unit, firstly putting the goose blank into pickling equipment for pickling, after pickling is completed, putting the goose blank into marinating equipment for marinating, obtaining the salted goose after marinating is completed, and entering a step of obtaining and detecting quality information of the salted goose; the quality information of the salted geese is obtained and detected through an information acquisition unit II, the quality information is compared with a database standard value to judge the product qualification, and the quality information comprises color and flavor information of the salted geese; and carrying out air drying treatment, vacuum sealing and sterilization treatment on the treated qualified salted goose finished product. The intelligent salted goose production purposes of high efficiency, energy conservation, stable quality control and nutrition enhancement can be achieved.

The invention belongs to the technical field of gene detection, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker, a primer pair, a kit, a detection method and application of a g.64930 site of a PPARG (Peroxisome Peroxisome Activated Receptor Gene) gene of Anhui local variety cattle. The nucleotide sequences of the molecular marker are as shown in SEQ ID NO.1 and SEQ ID NO.2, when the basic group at the 118th site of the 5'end of the molecular marker is G, the meat color brightness of beef is higher than that when the basic group at the site is T, and the muscle pH is lower than that when the basic group at the site is T. The molecular marker can be used as a candidate molecular marker for the quality character of the Anhui local variety cattle, is applied to population breeding of the Anhui local variety cattle, and is combined with production requirements for molecular-assisted breeding, so that a theoretical basis is conveniently provided for variety resource development and breeding of the Anhui local variety cattle.

The invention belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The molecular probe combination and the gene chip of the marker site combination for meat duck whole genome breeding simultaneously cover 7 representative meat duck varieties and 71 economic characters, have richer polymorphism and higher pertinence in meat duck groups, and are lower in cost and higher in speed compared with high-throughput sequencing detection; the breeding chip is designed according to the growth, feed efficiency, slaughtering, breeding, egg quality and various molecular phenotypes of the meat ducks, and compared with a high-throughput sequencing technology, the breeding chip is higher in seed selection accuracy, has higher breeding value, can be widely applied to breeding genotype detection of the meat ducks, and can be used for detecting the breeding genotypes of the meat ducks. And the method has creative significance in the aspect of meat duck genome selective breeding.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to a sheep body type as well as a KASP primer and application thereof, and belongs to the technical field of molecular markers. The SNP molecular marker related to the sheep body type is characterized in that a G / A polymorphic site exists at the 101st site on a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID NO: 1. A KASP primer is developed on the basis of the SNP molecular marker, the KASP primer is used for detecting the genotype of the SNP molecular marker in sheep, sheep individuals with AA genotype or AA genotype and GA genotype are removed, and sheep individuals with GG genotype are reserved. According to the method, an effective means is provided for breeding of sheep body type dominant varieties, the sheep body type can be enlarged by screening the sheep carrying the G type, breeding work is simplified, meanwhile, economic benefits of sheep breeding are increased, and the method has important significance.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC350094, the KASP type molecular marker 350094 closely linked with the cowpea salt tolerance major QTL is located at the 43398473 bp position on the No.7 chromosome, the marker can be used for amplifying a to-be-detected material, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the to-be-detected material. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention provides a local livestock and poultry variety identification method based on an SNP marker, and relates to the field of variety identification, and the method comprises the following steps: S1, genotype data acquisition, S2, genotype data quality control, S3, variety identification standard library establishment, and S4, bloodline content evaluation. According to the method, on the basis of a standardized molecular identification analysis process of genomic SNP marker bloodline content evaluation, genotype information of a core SNP site is analyzed, so that whole analysis process processing from original genotyping data to variety identity judgment is realized; the method can provide standardized, convenient and efficient molecular detection technical support for protection and utilization of genetic resources of local livestock and poultry in China, germplasm innovation and industrial development.

The invention provides application of a pig STR molecular marker combination in pig individual identification, a kit and an identification method, relates to the technical field of biology, and develops an STR molecular marker combination for accurate identification of pig individual identification by applying a whole genome association analysis method for 18 wild boar varieties and 42 domestic pig varieties. By detecting the combination, individual identification and genetic identification can be accurately and efficiently carried out on a to-be-detected pig sample, and when the minimum set number of the selected STR marker is 6, individual distinguishing can be carried out. According to the scheme provided by the invention, by comprehensively analyzing the STR typing result, the parent-child margin determination and individual identification of the pig can be effectively carried out, so that the pedigree accuracy in pig breeding work can be improved, the breeding process is accelerated, and the method has good application prospects and economic values.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300124, a KASP type molecular marker 300124 closely linked with the cowpea salt tolerance major QTL is located at the position of 43716091 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

A computing device may receive an inheritance dataset of a target domestic companion animal that belongs to a first owner, the first owner being a user of an online system. The computing device accesses inheritance datasets of reference panel animals. The reference panel animals are organized into breeds. The computing device compares the inheritance dataset of the target domestic companion animal to the inheritance datasets of the reference panel animals to identify breeds of the target domestic companion animal. The computing device identifies a plurality of matched domestic companion animals in the breeds of the target domestic companion animal. The computing device filters the matched domestic companion animals based on geographical proximity. The computing device causes to display a filtered matched domestic companion animal to the first owner of the target domestic companion animal to indicate potential social matches for the target domestic companion animal.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The application discloses a SNP marker related to an intramuscular fat content character of Rongchang pigs, a detection primer and application, and belongs to the technical field of molecular biology. The application develops a SNP marker and a detection primer related to the intramuscular fat content character of Rongchang pigs based on a SNP marker technology. The SNP marker developed by the application is located at 121626638 bp of a No. 7 chromosome of Rongchang pigs, and relates to C / T mutation. When the genotype is CT, the intramuscular fat content of Rongchang pigs is significantly higher than that of the TT genotype. The SNP marker can screen out individuals with excellent intramuscular fat content, so that the level of the intramuscular fat content character of Rongchang pigs is improved, and the breeding process of new breeds is accelerated.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker influencing the lambing number of goats. The marker is located at the 6293801th basic group on the No.15 chromosome of a goat, the specific SNP marker site is C / G basic group mutation at the 392bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of a CC genotype goat individual and a CG genotype goat individual at the site is remarkably higher than that of a GG genotype individual. The method comprises the following steps: by taking a Muncheng black goat as a research object, carrying out PCR (Polymerase Chain Reaction) amplification on a segment of ENSCHIG00000009743 gene, wherein the segment contains a goat 15 # chromosome g.6293801Cgt; according to the present invention, the DNA sequence of the G mutation site is analyzed, the influence of the specific genotype of the site on the lambing number of the Mancheng black goats is analyzed, and the individual breeding of the Mancheng black goats is performed according to the influence, such that the Mancheng black goat lambing number can be increased, and the breeding of the new variety (strain) using the Mancheng black goats as the breeding material can be accelerated; and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The invention belongs to the technical field of molecular biology, particularly relates to a pig chromosome 3 TMC7 gene as a meat-producing character molecular breeding marker and application, and aims to provide a new marker and technical support for meat-producing character molecular breeding of pigs. The molecular marker is located at the 26346502 base in a pig chromosome 3 TMC7 gene, and allele G / T polymorphism exists at the site. A correlation analysis result shows that in American and French large white pig populations, the eye muscle area of a GG genotype individual is remarkably higher than that of other genotype individuals (plt; 0.05), and it is indicated that the genotype is a favorable genotype of the eye muscle area character. The SNP marker provided by the invention provides a new molecular tool for genetic breeding of pig eye muscle area characters, and can be used for auxiliary screening of breeding pigs with excellent meat production performance, so that the breeding process of pig breeds with high lean meat percentage is accelerated.

The application provides a SNP primer combination for identifying a static chicken breed and application thereof, and belongs to the technical field of biology. Whether a to-be-detected individual belongs to a static chicken is judged by detecting the genotypes of seven SNP sites of the static chicken. The method is simple in operation, high in accuracy, can accurately identify the static chicken breed, and effectively strikes the fake and inferior products of the static chicken local breed in the market.

The present application relates to a SNP marker Ec62238 related to the reproductive traits of Exopalaemon modestus and an application thereof, and belongs to the technical field of crustacean genetics and breeding. Detection primers SEQ ID NO. 1-2 and typing primers SEQ ID NO. 3-5 are designed according to the SNP marker Ec62238. The gene sequence in which the SNP marker Ec62238 is located is shown in SEQ ID NO. 6. The base at the 601st site of the 5' end of the gene sequence is G or A. The SNP marker Ec62238 is located on the 24th chromosome of Exopalaemon modestus. The present application also provides an application of the detection primers and the typing primers in the selection of Exopalaemon modestus of good reproductive traits. The molecular marker is used for the screening of Exopalaemon modestus with high reproductive capacity or the selection of good breeds with high reproductive capacity, and can save cost and shorten the breeding period.

The present invention discloses a SNP molecular marker associated with the IMF trait of Ningxiang pigs and its application. The SNP molecular marker includes at least one of the SNP1 site, SNP2 site, SNP3 site, SNP4 site, SNP5 site, and SNP6 site. The present invention provides a SNP molecular marker associated with the IMF trait, which can efficiently and accurately identify and screen Ningxiang pig breeds with different IMF contents. It has the characteristics of rapid, accurate, and low-cost detection. It can be used for the detection and application of different Ningxiang pig breeds, providing an indispensable and important tool for the breeding of Ningxiang pig breeds with high IMF content and excellent meat quality, and for improving the quality of Ningxiang pork.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

The invention discloses a construction method of an MD-100K liquid phase chip for genome selection of dairy cattle and dairy-meat cattle. The construction method comprises the following steps: step 1, obtaining multiple varieties of candidate SNP (Single Nucleotide Polymorphism) sites; step 2, performing multiple quality screening on the SNP sites; and step 3, designing and optimizing the probe. The method has the beneficial effects that the method is specially designed for the main dairy cattle variety and the dairy and meat dual-purpose cattle variety in China for the first time, and the problem that imported chips are not suitable for water and soil in local varieties in China is effectively solved.

This invention discloses a dog lifting device (10) for animals particularly for big breed canine or dogs as a support during medical or surgical procedures but more particularly to a dog lifting device (10) which permits large and heavy dogs to be lifted with relative ease during periods of medical and surgical procedures, temporary or permanent paralysis. The dog lifting device (10) comprises a frame (12), a motored hoist (22) secured to the frame (12), an actuator (24) connected to the motored hoist (22) and a lifting rope (26) operably connected to the actuator (24) with a pulley (26) that translates the horizontal force from the motored hoist (22) to the lifting rope (26) which makes the dog lifting device (10) efficient, safe and easy to use with due regard to animal welfare.

The invention relates to application of an SNP site of a Yanbian cattle FABP2 gene in improving meat quality of Yanbian cattle, and belongs to the technical field of molecular biology. The SNP (Single Nucleotide Polymorphism) sites comprise one or more of a g.5970161 site, a g.5970209 site, a g.5972550 site, a g.5972551 site and a g.5973068 site, and are divided into five types of haplotypes, namely H001, H002, H003, H004 and H005 respectively. The five SNP loci of the Yanbian cattle FABP2 gene can regulate and control meat quality traits, provide reliable markers for molecular breeding of Yanbian cattle, accelerate polymerization and fixation of excellent alleles, realize accurate improvement of high-quality meat traits of Yanbian cattle, further select individuals carrying excellent genotypes for breeding, accelerate fixation and inheritance of excellent traits, and improve the breeding efficiency of Yanbian cattle. The Yanbian cattle variety with better meat quality is bred.

The invention relates to the technical field of molecular breeding, and discloses a molecular marker related to the LDL-C level of porcine serum, a detection reagent and a breeding method. The invention also discloses a primer group for identifying the molecular marker, and the primer group is used for amplifying the following SNP sites: SNP01, which are obtained on the basis of a pig reference genome Sscrofa11.1 and are positioned on 112bp, 008bp and 261bp of a third chromosome, and the genotype is A / G; sNP02 is located at 112bp, 008bp and 354 bp of a third chromosome, and the genotype of SNP02 is A / G; sNP03 is located at 112bp, 008bp and 583bp of the third chromosome, and the genotype of SNP03 is A / G. The primer group for identifying the molecular marker can quickly and effectively identify the LDL-C level of pig serum, and is used for assisting in breeding pig strains with low cholesterol or specific lipid metabolism characteristics.

The present application relates to the technical field of image detection, and can be applied to the business system platform of financial technology, medical health and the like, and discloses a non-contact living body feature data analysis method, device, equipment and medium, comprising: non-contact collection of a three-dimensional space scene and a reference age in a space within a radius range around a target living body; extraction of a living body contour area of the target living body from the three-dimensional space scene, determination of a mass measurement reference value of the target living body according to the area size of the living body contour area; generation of a measurement point position guide mark of the target living body according to the mass measurement reference value; calculation of living body length data and living body height data of the target living body by using the measurement point position guide mark, identification of living body breed information of the target living body by using a living body breed database; and calculation of a living body feature data analysis result of the target living body according to the reference age, the living body length data, the living body height data and the living body breed information. The present application can improve the accuracy of non-contact living body feature data analysis.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.