243 results about "Breed" patented technology

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The application provides a SNP primer combination for identifying a static chicken breed and application thereof, and belongs to the technical field of biology. Whether a to-be-detected individual belongs to a static chicken is judged by detecting the genotypes of seven SNP sites of the static chicken. The method is simple in operation, high in accuracy, can accurately identify the static chicken breed, and effectively strikes the fake and inferior products of the static chicken local breed in the market.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

The invention discloses a construction method of an MD-100K liquid phase chip for genome selection of dairy cattle and dairy-meat cattle. The construction method comprises the following steps: step 1, obtaining multiple varieties of candidate SNP (Single Nucleotide Polymorphism) sites; step 2, performing multiple quality screening on the SNP sites; and step 3, designing and optimizing the probe. The method has the beneficial effects that the method is specially designed for the main dairy cattle variety and the dairy and meat dual-purpose cattle variety in China for the first time, and the problem that imported chips are not suitable for water and soil in local varieties in China is effectively solved.

The invention relates to application of an SNP site of a Yanbian cattle FABP2 gene in improving meat quality of Yanbian cattle, and belongs to the technical field of molecular biology. The SNP (Single Nucleotide Polymorphism) sites comprise one or more of a g.5970161 site, a g.5970209 site, a g.5972550 site, a g.5972551 site and a g.5973068 site, and are divided into five types of haplotypes, namely H001, H002, H003, H004 and H005 respectively. The five SNP loci of the Yanbian cattle FABP2 gene can regulate and control meat quality traits, provide reliable markers for molecular breeding of Yanbian cattle, accelerate polymerization and fixation of excellent alleles, realize accurate improvement of high-quality meat traits of Yanbian cattle, further select individuals carrying excellent genotypes for breeding, accelerate fixation and inheritance of excellent traits, and improve the breeding efficiency of Yanbian cattle. The Yanbian cattle variety with better meat quality is bred.

The invention relates to the technical field of molecular breeding, and discloses a molecular marker related to the LDL-C level of porcine serum, a detection reagent and a breeding method. The invention also discloses a primer group for identifying the molecular marker, and the primer group is used for amplifying the following SNP sites: SNP01, which are obtained on the basis of a pig reference genome Sscrofa11.1 and are positioned on 112bp, 008bp and 261bp of a third chromosome, and the genotype is A / G; sNP02 is located at 112bp, 008bp and 354 bp of a third chromosome, and the genotype of SNP02 is A / G; sNP03 is located at 112bp, 008bp and 583bp of the third chromosome, and the genotype of SNP03 is A / G. The primer group for identifying the molecular marker can quickly and effectively identify the LDL-C level of pig serum, and is used for assisting in breeding pig strains with low cholesterol or specific lipid metabolism characteristics.

The present application relates to the technical field of image detection, and can be applied to the business system platform of financial technology, medical health and the like, and discloses a non-contact living body feature data analysis method, device, equipment and medium, comprising: non-contact collection of a three-dimensional space scene and a reference age in a space within a radius range around a target living body; extraction of a living body contour area of the target living body from the three-dimensional space scene, determination of a mass measurement reference value of the target living body according to the area size of the living body contour area; generation of a measurement point position guide mark of the target living body according to the mass measurement reference value; calculation of living body length data and living body height data of the target living body by using the measurement point position guide mark, identification of living body breed information of the target living body by using a living body breed database; and calculation of a living body feature data analysis result of the target living body according to the reference age, the living body length data, the living body height data and the living body breed information. The present application can improve the accuracy of non-contact living body feature data analysis.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the egg yield character of Muscovy ducks and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, and Cgt exists in the 62nd basic group from the 5'end of the sequence; t mutation. A primer pair and a KASP primer group for amplifying and detecting the SNP molecular marker, wherein the sequences of the primer pair and the KASP primer group are as shown in SEQ ID NO: 2-6; the SNP molecular marker, the primer pair, the KASP primer group and the like are applied to auxiliary identification and breeding of high-egg-yield muscovy duck varieties. The CDC20 gene SNP molecular marker related to the Muscovy duck egg production traits is explored in Muscovy ducks for the first time, a new molecular marker is provided for molecular marker-assisted selective breeding, and a scientific basis is provided for breeding of new Muscovy duck strains with excellent reproduction traits; the CC genotype egg yield of the site is higher than that of the CT genotype and that of the TT genotype, and high-egg-yield Muscovy duck individuals can be quickly screened through early genotyping.

The embodiment of the present application relates to the technical field of breeding management, and discloses a selection and breeding method based on multidimensional data, which comprises the following steps: determining the feather characteristic parameters of chicks according to feather characteristic information and pixel point size; determining the gender and predicting the growth parameter information of the corresponding chicks according to the length of the primary wing feather and the length of the over primary wing feather, and performing primary screening according to the predicted growth parameter information; inputting the image information of the breeding hen into a feature recognition model to obtain the corresponding characteristics of the breeding hen; inputting the comb image into a comb recognition model to determine the corresponding comb type; if the feather color, the chicken toe color and the comb type match the breed parameters of the corresponding breeding hen, then the breeding hen is kept and caged, and the individual characteristic parameters are recorded. The scheme of the embodiment of the present application realizes the deep fusion of image recognition technology and multidimensional phenotype data, constructs a full-cycle accurate selection and breeding system from chicks to breeding hens, and realizes the automation of gender identification, the standardization of breed verification and the intelligentization of growth prediction.

The invention discloses a molecular marker related to correction day age of 100 kg weight of a pig and application of the molecular marker, and belongs to the technical field of molecular genetics. According to the invention, for the first time, two SNP loci obviously associated with the weight correction age of a pig reaching 100 kg are identified in a CDS region of a GREB1 gene, and the two SNP loci show complete linkage imbalance and form a conservative haplotype block. The two mutation sites are significantly related to the corrected day age (Plt; 0.001) of the 100 kg weight of the pig, and the corrected day age of the GA genotype is significantly shorter than the corrected day age of the GG genotype and the AA genotype. Detection of the molecular marker associated with the correction day age reaching 100 kg is beneficial to breeding of meat varieties / lines with high growth speed, and has important significance for breeding of pig varieties.

The invention relates to the technical field of molecular detection, and particularly discloses a police dog 10K liquid phase chip, a design method and application thereof and a kit, and genetic typing sites of the police dog 10K liquid phase chip comprise 10463 SNP sites; the physical positions of the 10463 SNP loci are determined based on the comparison of the whole genome sequence of the canine reference genome CanFam 3.1, and the 10463 SNP loci are shown in the table 1. The 10463 SNP loci disclosed by the invention are obtained by screening based on a DNA sample of the police dog, and the obtained 10463 SNP loci can be used as a basis for genetic typing of the police dog; the liquid chip designed according to 10463 SNP loci can quickly realize genotyping of the target SNP loci, and obtained genotyping data can be used for variety identification of police dogs and population diversity analysis of police dogs.

The application discloses a SNP molecular marker combination of a FGF9 gene promoter region related to chicken egg laying traits and application thereof, and belongs to the technical field of molecular genetics. The application finds that multiple SNP sites exist in the FGF9 gene promoter region of a chicken through screening of SNPs between breeds, and for the first time, carries out correlation analysis on the SNPs genotypes of a Jining Hundred-day chicken population and egg laying traits, finds that the GA ‑2177 genotype individual corresponds to more maximum consecutive egg laying numbers; the GA ‑2289 genotype individual corresponds to an earlier age of first egg laying; and the GA ‑3906 genotype individual corresponds to more total egg laying numbers. Therefore, through detection of the three molecular markers related to the egg laying traits, not only is the method simple and fast, but also it is helpful to breed a high egg laying chicken breed, and provides favorable help for breeding work.

The application relates to the field of animal genetic breeding technology, and discloses a local pig breed multi-node block chain collaborative breeding method. According to the method, key data abstracts, evaluation results, model versions and breeding decisions are chained and left marks in a window period through a permissioned consortium chain and a consensus verification mechanism, the source is clear, tamper-proof and auditable, the problem that data standards are different, difficult to trace and difficult to trust in traditional multi-subject collaboration is solved, in addition, an AI multi-factor breeding evaluation system can simultaneously process multiple traits such as meat quality, growth, reproduction, health resistance and environmental management factors, can depict the nonlinear correlation between complex traits, can improve the evaluation precision and stability of the comprehensive genetic value of local pig breeds, can reduce the risk of decision-making errors caused by single-point data or single-algorithm deviation, can realize cross-node alignment and joint use of dispersed data through a rolling window and a labeled archive, and can make up for the short board of insufficient samples of a single breeding unit.

The application discloses an InDel molecular marker combination, primers thereof and application of the molecular marker combination in identifying Hubei Enshi yellow cattle breeds, and the molecular marker combination comprises four InDel molecular markers, namely InDel_2 located at 88012237 bp of a bovine chromosome 1, the nucleotide sequence of which is TAAGA, InDel_4 located at 59170765 bp of a bovine chromosome 11, the nucleotide sequence of which is G, InDel_8 located at 29286667 bp of a bovine chromosome 8, the nucleotide sequence of which is G, and InDel_14 located at 15978630 bp of a bovine chromosome 2, the nucleotide sequence of which is AGC. The molecular marker combination can be used as an effective target for identifying the bloodlines of existing bovine breeds, and is used for identifying Hubei Enshi yellow cattle and foreign bovine breeds or hybrid bovine breeds containing foreign bovine breed bloodlines. If the above characteristics are met, the bovine breed is Hubei Enshi yellow cattle; and if the above characteristics are not met, the bovine breed is a foreign bovine breed or a hybrid bovine breed containing a foreign bovine breed bloodline. The identification method provides necessary resources and methods for analyzing the bloodline sources of bovine breeds and subsequent crossbreeding improvement, and the identification method is simple, accurate and effective.

The application provides a genetic marker associated with chicken intestinal length in a KCNIP4 gene and an application thereof, and belongs to the fields of animal genetics and breeding and biotechnology.The genetic marker comprises IL_tag1 or IL_tag2; the Ensembl number of the IL_tag1 is rs316532738, corresponds to the sequence of a positive strand of a chromosome No.4 of a chicken reference genome bGalGal1.mat.broiler.GRCg7b published by NCBI, is located in the 1st intron of a gene KCNIP4, and the base at the position is T or C; the Ensembl number of the IL_tag2 is rs316953671.The genetic marker is helpful to genetically improve the intestinal length of a laying hen, is applied to the genetic breeding of a chicken, and is favorable to improving intestinal traits and obtaining a laying hen variety with better nutrient absorption.

This invention discloses a method for breeding a new dual-purpose (meat and milk) sheep breed. The method involves crossbreeding East Friesian sheep as the sire and Lowland sheep as the dam to obtain the East F1 generation; crossbreeding the East F1 generation with Lowland sheep as the dam to obtain the East F2 generation; crossbreeding the East F1 generation with East Friesian sheep as the dam to obtain the East F2 generation flock; and crossbreeding the East F2 generation with East F1 generation as the dam and Lowland sheep as the dam to obtain the East F3 generation flock. Superior rams and ewes from the East F3 generation flock are selected for self-pollination to establish a zero-generation dual-purpose sheep flock. Through crossbreeding fixation, generational selection, and breeding expansion, a new dual-purpose sheep breed is developed. The new breed developed by this invention has advantages such as good meat performance, excellent milk production performance, high fertility, good maternal instincts, and strong adaptability.

This invention discloses a kit and method for evaluating the growth traits of Dwarf Chickens. Using Dwarf Chickens as the research subject, this invention identifies six SNP loci on the IGF2BP3 gene of Dwarf Chickens that are significantly associated with growth traits such as body weight, keel length, chest width, chest depth, shank length, or body oblique length. These SNP loci can serve as molecular breeding markers for Dwarf Chicken growth traits. By detecting these SNP loci, the body weight and body shape of Dwarf Chickens can be predicted rapidly, effectively, and accurately at an early stage, shortening breeding time and accelerating the breeding process. This invention provides fundamental data for future molecular breeding and breed improvement of Dwarf Chickens, and can significantly improve the efficiency of Dwarf Chicken germplasm resource improvement.

The application relates to the technical field of feed formula data optimization, in particular to a pig feed formula data optimization processing method and system based on a computer, which comprises the following steps: acquiring parameters corresponding to a target breeding scene and inputting the parameters into a preset collaborative optimization model; wherein the collaborative optimization model is configured to: determine a reference threshold of digestible protein; dynamically compensate the reference threshold to generate an upper limit of dynamic demand of the digestible protein; determine a soluble fiber proportion target coordinated with the upper limit of dynamic demand; take the received upper limit of dynamic demand of the digestible protein and the soluble fiber proportion target as a collaborative optimization target, perform proportioning calculation on raw material available nutrient component parameters, and generate a final feed formula meeting the collaborative optimization target. The application can construct a collaborative optimization model of pig breed genetic characteristics, environmental temperature and raw material available nutrients, and realize dynamic and accurate adaptation of a feed formula.

The application discloses a SNP molecular site, a detection primer thereof and application in predicting high and low of egg laying traits of chickens. The application compares and analyzes SNP typing data of different egg laying performance chicken breeds, and carries out association analysis by combining phenotype data related to egg laying traits, finally identifies a SNP marker site significantly related to egg laying number of chickens at 300 days old, which corresponds to sequence information 61,577,523 bp of chromosome 1 of a chicken reference genome GRC7b version published in an Ensembl website, wherein a base at the position is A or G, and the SNP number is rs740376220. The application further provides a detection primer for detecting the genotype of the SNP molecular site. The SNP marker site and the detection primer thereof can be used for accurately predicting high and low of egg laying traits of chickens, and can provide technical support for molecular marker assisted selection breeding of egg laying traits of chickens, shorten breeding period and improve breeding efficiency.

The invention provides an SNP (Single Nucleotide Polymorphism) marker primer pair related to pig multirib traits and application of the SNP marker primer pair. The SNP site is a nucleotide site of chromosome 7 rs701714758 of a reference sequence of an international pig genome version 11.1, has G / A polymorphism, is located in a protein coding region of an ABCD4 gene, and is remarkably associated with rib number characters of Duroc pigs, landrace pigs, large white pigs and Pietrain pigs through verification of whole genome association analysis and meta analysis. The invention further provides a primer pair for detecting the SNP marker, an upstream primer of the primer pair is SEQ ID NO: 2, and a downstream primer of the primer pair is SEQ ID NO: 3. The SNP marker can be applied to marker-assisted selection and whole genome selection of rib number characters of multiple varieties of pigs, can accurately screen a multi-rib number pig group, improves character prediction accuracy, increases pork and rib row yield, meets consumption demands, and has both social and economic benefits.

The application discloses a sheep meat tracing method based on mineral fingerprint characteristics and belongs to the technical field of food tracing detection. The method takes the longissimus dorsi muscle of Yemule Baiyang, Kazakh sheep and Turpan black sheep in northern Xinjiang as samples, determines the content of 28 kinds of mineral elements by using an ICP-MS method, screens ten kinds of elements B, Na, Mg, V, Zn, As, Rb, Sr, Mo and Ag based on Wilks' Lambda criterion, and constructs a stepwise linear discriminant model, wherein the discriminant accuracy of model back substitution and cross validation reaches 100%. The method has strong pertinence, high precision and good stability, can quickly and accurately identify three kinds of characteristic sheep meat, and provides a reliable technical means for Xinjiang northern characteristic sheep meat origin tracing, variety identification, brand protection and market supervision.

The application discloses a specific SNP site primer combination for identifying Taihe black-bone chicken varieties and application thereof. A chicken pan-genome containing a chicken reference genome GRCg7b and 2.49 Gb pan-sequence is taken as reference, WGS data of Taihe black-bone chicken and related chicken varieties is analyzed and screened to obtain two specific SNP sites, namely novel_th1 (G / T, dominant allele G) and novel_th2 (C / T, dominant allele C), wherein the T allele is medium to high frequency in most non-Taihe black-bone chicken varieties / strains. A FAM / VIC double-labeled TaqMan probe and a matching primer are designed based on the two sites to construct a real-time fluorescent quantitative PCR detection kit for joint typing of the two sites, and an amplification system and a Ct value judgment standard are established, so that 96 varieties / strains such as Taihe black-bone chicken, Zhushi chicken, other black-bone chicken, local chicken, white-feathered broiler and egg chicken can be quickly and accurately identified. The method has the advantages of high specificity, high sensitivity, simple operation and low cost, and is suitable for identification of authenticity of Taihe black-bone chicken and products thereof, purity monitoring and molecular-assisted breeding.

The utility model relates to the technical field of novel livestock and poultry large-scale breeding, in particular to a meat duck cub separating device which comprises an incubator shell, a conveying belt is arranged in the incubator shell, and a separating assembly is arranged at the top of the conveying belt; the servo motor drives the spiral telescopic rod, and the distance between the heating plate and the duck egg placement base can be accurately controlled. Different varieties of meat duck eggs have different requirements on temperature in the hatching process, the temperature distribution in the hatching chamber can be accurately adjusted by adjusting the position of the heating plate, the duck eggs are always in the most suitable hatching temperature environment, the hatching success rate is greatly improved, and the hatching quality is improved for some varieties sensitive to temperature. The height of the heating plate can be adjusted in time according to the requirement of a specific incubation stage, the duck egg placement seat can be easily moved through the sliding rail in the incubation process, damage possibly caused by duck egg movement is avoided, and meanwhile operation convenience is improved.

The invention relates to molecular identification of pig varieties, and discloses an SNP molecular marker combination for identification of six white pigs in Anqing, the combination is composed of the following 14 SNP sites, and the physical position and allele information of each SNP site are determined based on a pig reference genome Sscrofa11.1: rs340865310 located at 226716bp of a chromosome 2, the allele of which is G / A; the rs344868753 is located at the 124988228bp position of the chromosome 2, and the allele of the rs344868753 is T / C; the rs793459202 is located at the 12758234 bp position of the chromosome 3, and the allele of the rs793459202 is C / T; and the rs700761784 is located at the 13345574bp position of the No.3 chromosome, and the allele of the rs700761784 is C / T. According to the invention, 14 specific SNPs are taken as a marker set, and unified 0 / 1 / 2 codes, standardized parameters mu / sigma and threshold judgment rules are matched, so that the flow of a detection end is clear, the repeatability is high, and cross-laboratory consistent implementation is facilitated.

This invention relates to a method for breeding dwarf and black-legged chickens and its application. The breeding method includes the following steps: breeding D, E, and C lines respectively, using D line as the first paternal line, E line as the terminal paternal line, and C line as the maternal line; hybridizing the first paternal line D line with the maternal line C line to obtain parent generation hens; and hybridizing the parent generation hens with the terminal paternal line E line to breed dwarf and black-legged chickens. This invention introduces sex-linked dwarfism genes into the Iron-Leg Ma chicken breed to breed sex-linked dwarf Iron-Leg Ma chickens. Simultaneously, it pairs high-reproductive-performance recessive White Locker chickens with black-legged Ma chickens, resulting in commercial chickens suitable for markets in Southwest and Northern China, possessing characteristics such as black legs, dwarf legs, white meat, fast growth rate, and high feed conversion rate.

This invention discloses a molecular marker associated with the chicken shank length trait and its application, belonging to the field of poultry breeding technology. This invention selected 505 Ma Huang chickens as experimental subjects for genome-wide association analysis (GWAS). Shank length trait data were collected, and blood samples were collected from under the wings at 77 days of age to extract genomic DNA. The integrity, purity, and concentration of the extracted genomic DNA were tested. Qualified samples were resequencing, and then GWAS was performed on the shank length trait, detecting 458 SNPs significantly associated with shank length. Further validation analysis was conducted on some of these SNP sites and their association with the shank length trait, providing theoretical support for breeding chicken breeds associated with the shank length trait.