181 results about "Linolenic acid" patented technology

The invention discloses a preparation method of linolenic acid structural ester with synergistic biological activity, and relates to the technical field of linolenic acid structural ester preparation.The preparation method comprises the steps that specific lipase is prepared through rhizopus wei, and after an SBA-15 molecular sieve is treated, the lipase is fixed to prepare a composite catalyst; the preparation method comprises the following steps: after hydroxyl protection of quercetin, esterifying quercetin with EPA to obtain quercetin-EPA ester; esterifying glycerol and linolenic acid in the presence of a composite catalyst and microwaves, and purifying to obtain an intermediate product; carrying out microwave catalytic ester exchange on the intermediate product and quercetin-EPA ester in tert-butyl alcohol through a composite catalyst to obtain a crude product; and removing impurities from the crude product by a urea adduction method, and carrying out molecular distillation graded purification, column chromatography, deodorization and filtration to obtain a final product. According to the invention, linolenic acid, quercetin and EPA are combined through molecular design to form a compound structure ester with a multi-target effect, and the linolenic acid, quercetin and EPA achieve functional complementation through a synergistic effect, so that the biological activity of the compound structure ester is enhanced.

The invention discloses a preparation method of fermented peony seed oil. The fermentation process is optimized, different strains such as lactic acid bacteria, saccharomycetes and bifidobacteria are innovatively combined for multiple times of fermentation, and the lactic acid bacteria and saccharomycetes mixed strains are used for primary fermentation, so that components are preliminarily decomposed, and the viscosity is reduced; a bifidobacterium strain is used for secondary fermentation, so that the components are further optimized. After fermentation, supercritical CO2 extraction and freezing purification are performed to obtain the high-quality peony seed oil. According to the process, the content of monounsaturated fatty acid oleic acid in the peony seed oil is increased to 80.06%, the content of polyunsaturated fatty acid linoleic acid and linolenic acid is reduced, the product stability is improved, the skin feeling is fresh and cool when the peony seed oil is used, the permeation rate is increased, the DPPH free radical scavenging rate is increased, and the peony seed oil has an anti-eczema effect.

The invention provides a preparation method and application of high alpha-linolenic acid, and relates to the technical field of medicines. Comprising the following steps: selecting purple perilla seeds, cleaning, drying, mechanically grinding in a protective atmosphere, and filtering to obtain purple perilla seed crude oil; carrying out degumming, deacidification, decoloration and dewaxing treatment on the crude perilla seed oil in a protective atmosphere to obtain primarily enriched crude alpha-linolenic acid oil; and respectively carrying out supercritical CO2 extraction, enzymatic molecular distillation and preparative chromatographic separation on the preliminarily enriched perilla seed oil to obtain the high alpha-linolenic acid. According to the method, purple perilla seeds are subjected to multi-stage treatment, extraction and refinement are performed layer by layer through three steps of supercritical CO2 extraction, enzymatic molecular distillation and preparative chromatographic separation, free fatty acid in purple perilla seed crude oil can be effectively removed, and alpha-linolenic acid is enriched to obtain high alpha-linolenic acid with the purity higher than 96%. The method is suitable for mass production in the medicine field.

This invention belongs to the field of genetic engineering technology, specifically involving GmDREB2A2 Application of genes in regulating fatty acid content in plants. Overexpression. GmDREB2A2 Gene regulation of fatty acid content in plants. This invention utilizes an exogenous gene sequence expression vector to express the coding sequence shown in SEQ ID NO.1. GmDREB2A2 Gene sequences are introduced into plants to enable GmDREB2A2 Gene overexpression. Under these conditions, the plant's linolenic acid, linoleic acid, and palmitic acid content were significantly higher than the control, while the content of eicosatrienoic acid and erucic acid was significantly reduced.

The invention discloses a composite material as well as a preparation method and application thereof, and relates to the technical field of high polymer materials. The composite material comprises the following components in parts by weight: 10-50 parts of epoxy resin; 10-20 parts of vegetable oleic acid; 1-3 parts of sulfur; 70-90 parts of a modified heat-conducting filler; the modified heat-conducting filler is obtained by modifying a heat-conducting filler through a double-bond-terminated silane coupling agent; the vegetable oleic acid comprises at least one of eleostearic acid, linolenic acid, linoleic acid, soybean oleic acid, oleic acid, ricinoleic acid and undecylenic acid. The composite material disclosed by the invention not only has the characteristics of high thermal conductivity and excellent electrical insulation performance, but also has good hot working performance and recycling capability, and shows a wide application prospect in the fields of high-thermal-conductivity green electronic packaging materials, electrical insulation coatings and sustainable composite materials.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

The invention relates to the technical field of functional edible oil processing, and discloses zanthoxylum oil with an auxiliary anticancer function prepared on the basis of an aqueous enzymatic method, and the zanthoxylum oil is prepared from the following raw materials in parts by weight: 88-95 parts of zanthoxylum seed oil, 3-8 parts of high-purity oleoresin, 0.6 part of compound enzyme, 1-4 parts of volatile essential oil and 0.05 part of a stabilizer, the preparation method of the zanthoxylum oil with the auxiliary anticancer function based on the aqueous enzymatic method is characterized by specifically comprising the following steps: 1, mixing and adjusting the pH value: crushing zanthoxylum seeds, mixing the crushed zanthoxylum seeds with drinking water, and adjusting the pH value to 5.5; 2, enzymolysis: adding a compound enzyme, and carrying out enzymolysis at 50 DEG C for 4 hours; according to the present invention, through the specific raw material ratio and preparation steps, the low temperature enzymolysis process is adopted, the activity of alpha-linolenic acid and other heat-sensitive components is effectively retained, the composite enzymolysis cooperates with the steam distillation to achieve the efficient extraction and compounding of a variety of active components, and the anti-cancer function of the zanthoxylum oil is effectively improved.

Methods and compositions comprising free fatty acids for the treatment, including prevention, of viral infections, including the treatment of Acute Respiratory Distress Syndrome (ARDS). The compositions comprising free fatty acids, optionally linolenic acid, linoleic acid, and / or palmitic acid, may be used in methods for treating viral infections, optionally, coronavirus infections, for example, SARS-CoV, MERS-CoV, and / or SARS-CoV-2 / COVID-19, and influenza virus infections, optionally, H1N1 and / or H5N1.

This invention discloses a low-temperature reverse flotation collector for collophane and its preparation method, belonging to the field of collophane flotation technology. The low-temperature reverse flotation collector for collophane of this invention is prepared from the following raw materials: linoleic acid, linolenic acid, and gallic acid. This invention generates a low-temperature reverse flotation collector for collophane containing two OH groups and one COOH group through an esterification reaction of linoleic acid, linolenic acid, and gallic acid. In acidic pulp, the flotation recovery rate is 20-50% lower than before the esterification reaction, and the dosage is significantly improved, resulting in a substantial increase in concentrate P2O5 grade and recovery rate. Furthermore, the low-temperature reverse flotation collector for collophane can be used for flotation at a low temperature of 5-10℃. The flotation foam generated during the flotation process has a short duration, low foam stability, and is easily defoamed, which is beneficial for continuous operation and tailings recovery.

The invention discloses China-hemp peptide multi-nutrient protein powder with a metabolism improving function, which comprises China-hemp seeds, soybean protein isolate, China-hemp seed protein, chickpea protein, pea protein, China-hemp peptide, rice protein, broad bean protein, mung bean protein and wheat hydrolyzed protein. Wherein the Chinese hemp seeds are 15-25 parts, the soybean protein isolate is 10-20 parts, the Chinese hemp seed protein is 5-15 parts, the chickpea protein is 8-12 parts, the pea protein is 8-12 parts, the Chinese hemp peptide is 8-15 parts, the rice protein is 5-10 parts, the broad bean protein is 5-10 parts, the mung bean protein is 5-10 parts, and the like. According to the present invention, besides the protein nutrition, the small molecule peptide and 10 other nutrients such as Omega3, gamma-linolenic acid, vitamin E and a variety of minerals are contained, such that the complete protein nutrition is provided, and the health-care food is rich in Omega-3 fatty acid, gamma-linolenic acid, vitamin E, potassium, magnesium, zinc and other minerals with different molecular magnitudes extracted from China-hemp seeds, the ten nutrients, a plurality of plant proteins and small molecule peptides have a synergistic effect to jointly form a basis for improving the metabolic function.

A perilla oil α-linolenic acid microcapsule and its preparation method belong to the food processing field, including the following steps: (1) extraction of perilla oil; using a bio-enzyme-assisted low-temperature pressing method, a compound enzyme is added to perilla seeds for enzymatic hydrolysis, followed by low-temperature pressing to collect perilla seed oil; (2) enrichment and purification of α-linolenic acid; the perilla seed oil obtained in step (1) is purified by molecular distillation technology, and the perilla oil fraction enriched with α-linolenic acid is collected; (3) microencapsulation; using the perilla oil enriched with α-linolenic acid obtained in step (2) as the core material, after selecting a suitable composite wall material, perilla oil α-linolenic acid microcapsules are obtained by spray drying. This invention improves the extraction efficiency of perilla oil, retains the natural activity of α-linolenic acid; efficiently enriches α-linolenic acid through molecular distillation technology, increasing the content of functional active ingredients in the product; and improves the oxidative stability and storage performance of α-linolenic acid.

The invention provides an extraction method of high-content alpha-linolenic acid, and relates to the technical field of biological extraction. The extraction method of the high-content alpha-linolenic acid comprises the following steps: pretreating raw materials, destroying a plant cell wall structure through related operations of crushing and degreasing, releasing grease components, improving the subsequent extraction efficiency, removing phospholipid and free fat, and avoiding impurities influencing the purification effect; the method comprises the following steps: carrying out directional hydrolysis on lipase, cutting off sn-1 and 3 site ester bonds by utilizing lipase specificity, converting triglyceride into free fatty acid, reserving the structural integrity of sn-2 site alpha-linolenic acid, and enabling free alpha-linolenic acid to be more easily separated through subsequent distillation / chromatography compared with glyceride form. Through a silver ion complexing chromatography technology, alpha-linolenic acid and other unsaturated fatty acids with similar structures can be accurately separated, the purity of the final product stably reaches 98% or above, the pharmaceutical grade application standard is met, and the technical bottleneck that a traditional purification method is difficult to break through the purity of 90% is solved.

The invention discloses a cell culture medium supplement as well as a preparation method and application thereof, and relates to the technical field of cell biology. The supplement comprises DL-alpha-tocopheryl acetate, linolenic acid, ethanolamine, L-carnitine hydrochloride, 3, 3 ', 5-triiodoL-sodium thyrosinate, and serum albumin. As a cell culture additive with definite chemical components and simple composition, the cell culture additive can replace a traditional culture system with unknown chemical components or too complex composition, and is used for supporting proliferation, differentiation and characterization analysis of cells. The culture medium additive is clear in chemical component, simple to prepare, stable in effect and relatively low in cost, can promote in-vitro proliferation and oxidation resistance of cells including human embryonic stem cells and mesenchymal stem cells, and has important application value in actual production.

A heart boosting formulation comprises: L-carnitine, alpha-linolenic acid, citrulline, vitamin D3, lycopene, holy basil, cinnamon and beetroot.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The invention discloses a composition for comprehensive conditioning of an internal environment, which comprises the following components in parts by weight: 1000-1200 parts of alpha-linolenic acid, 100-300 parts of cordyceps militaris powder, 50-150 parts of oyster powder, 50-150 parts of ginseng powder, 50-150 parts of pueraria peptide, 50-150 parts of fresh lalang grass rhizome powder and 50-150 parts of polygonatum odoratum powder. Wherein the purity of the alpha-linolenic acid is not lower than 80%. According to the invention, high-purity alpha-linolenic acid and six specific medicinal and edible components are scientifically proportioned to construct a multi-level synergistic system, and all the components complement and synergism on a plurality of targets such as lipid lowering, circulation promoting, sugar stabilizing, acid discharging, repairing and the like, so that comprehensive conditioning on disorder of multiple indexes such as blood fat, blood sugar, uric acid, blood pressure and the like is realized; the limitation of a single component or a simple compound product is broken through, and the comprehensive synergistic conditioning of the internal environment is realized; all the components are selected from a medicinal and edible substance catalog, so that the traditional Chinese medicine composition is safe and mild, meets the physique requirements of Chinese people on warm nourishing and conditioning, gives consideration to conditioning and repairing, and is high in adaptability.

This invention discloses a biological combination preparation for controlling crop pests and its application, belonging to the field of agricultural biotechnology. The biological combination preparation includes the plant inducer alpha-linolenic acid (ALA) and the pesticide chlorantraniliprole. For application, each 1g of dry seeds is thoroughly mixed with 1mL of an ALA solution at a concentration of 4-400mg / L and 2.5-5mg of a 20% chlorantraniliprole suspension before sowing. This invention is the first to utilize ALA in synergistic seed treatment with chemical pesticides, achieving a high lethality against pests while reducing the amount of chemical pesticides used. The treatment method of this invention does not affect seed germination rate or crop growth and development; it reduces the amount of chemical pesticides used by half compared to conventional seed treatment methods; and it is an intensive, simple, and labor-saving operation with significant social and ecological benefits.

The invention belongs to the technical field of biological medicine, and particularly relates to a diglycerol linolenate galactoside compound in pepper as well as a preparation method and application of the diglycerol linolenate galactoside compound. The preparation method comprises the following steps: (1) taking red peppers to prepare pretreated red peppers; (2) adding the pretreated red peppers into an ethanol solution, performing ultrasonic extraction, and collecting first filtrate and first filter residues; (3) adding an ethanol solution into the first filter residue, carrying out ultrasonic extraction, combining the first filtrate and the second filtrate, and concentrating to obtain a crude extract; (4) separating the crude extract by adopting high-speed counter-current chromatography to obtain a primary purified product; and (5) further separating and purifying the primary purified product by adopting high performance liquid chromatography, and identifying to obtain the diglycerol linolenate galactoside compound. The purity of the diglycerol linolenate galactoside compound obtained by the preparation method is as high as 99.3%, and the diglycerol linolenate galactoside compound has a remarkable scavenging effect on DPPH free radicals, shows good antioxidant activity and has a wide application prospect.

The invention discloses a water-soluble fertilizer for promoting swelling and coloring of crop fruits. The water-soluble fertilizer is prepared from the following raw materials: 30 to 50 parts of herba portulacae fermentation liquor, 15 to 25 parts of potassium nitrate, 10 to 20 parts of monopotassium phosphate, 2 to 8 parts of chelated trace elements, 2 to 4 parts of polyglutamic acid, 1 to 3 parts of compound sodium nitrophenolate and 1 to 3 parts of betaine. The invention belongs to the technical field of agricultural fertilizers, and particularly provides a water-soluble fertilizer for promoting fruit swelling and coloring of crops, which is characterized in that inert precursor substances in purslane are converted into efficient active components by a fermentation technology, and alpha-linolenic acid derivatives generated by fermentation of the purslane stimulate expression of relaxation proteins in fruit cell walls to achieve a fruit swelling effect.

This invention relates to ketogenic nutritional powder containing medium- and long-chain fatty acid triglycerides, its preparation method, and its application. It belongs to the field of food science and engineering technology. It uses enzymatically synthesized medium- and long-chain fatty acid triglyceride structured lipids as the sole fat source, which is different from the traditional physical mixture of long-chain triglycerides and medium- and long-chain oils. Medium-chain fatty acids and long-chain fatty acids are simultaneously bound to the same glycerol molecule. Fatty acid digestion and absorption are synchronized and metabolized, which can give full play to the synergistic nutritional advantages. It retains the characteristics of medium-chain fatty acids that provide rapid energy and are not easy to accumulate in body fat, while also supplementing essential fatty acids such as linoleic acid and α-linolenic acid. At the same time, it overcomes the disadvantages of pure medium-chain fatty acid oils that cause great gastrointestinal irritation and have a low smoke point.

The invention belongs to the technical field of microcapsules, and particularly relates to an alpha-ethyl linolenate microcapsule and a preparation method thereof. The invention provides an alpha-ethyl linolenate microcapsule which comprises a core material and a wall material, and the core material comprises alpha-ethyl linolenate; the wall material comprises soybean protein isolate, maltodextrin and microcrystalline cellulose; the mass ratio of the soybean protein isolate to the maltodextrin to the microcrystalline cellulose is (3: 1)-(1.2: 1); and the mass ratio of the core material to the wall material is 1: (2-3). The wall material composed of the soybean protein isolate, the maltodextrin and the microcrystalline cellulose is more effective in the aspect of enhancing the interface structure, so that the wall material has a better protection effect on unsaturated fatty acid.

The invention discloses animal and plant blend oil conforming to a dietary fatty acid equilibrium standard and a preparation process thereof, and belongs to the technical field of edible oil and fat processing. The blend oil is prepared from the following components in parts by mass: 5 to 30 parts of low-cholesterol animal oil, 70 to 95 parts of various vegetable oil and 0.001 to 0.015 part of an emulsifier RXLY-2. The method is characterized in that the fatty acid composition of the final product simultaneously meets the condition that the mass ratio of saturated fatty acid to monounsaturated fatty acid to polyunsaturated fatty acid is (0.95-1.05): (0.95-1.05): (0.95-1.05), and the mass ratio of linoleic acid to alpha-linolenic acid is 4: 1-6: 1. The preparation process comprises the following steps: preheating and emulsifying the animal oil, mixing with the vegetable oil, heating and curing, filtering and filling and the like. According to the invention, through scientific proportioning and a specific process, the internationally recognized'double gold proportion 'of dietary fatty acids is accurately realized, the problems of unbalanced nutrition and imbalance ratio of essential fatty acids of single edible oil are solved, and the product has good stability and is suitable for long-term eating by various people.

This invention discloses a blueberry anthocyanin nutritional solid composition for relieving eye discomfort. The composition comprises blueberry anthocyanin solid powder, algal oil powder, zinc gluconate, and selenium-enriched yeast, and may optionally contain lipid nutrients containing gamma-linolenic acid, as well as food additives and excipients. Oral administration of this composition provides the body with various nutritional supports related to ocular surface function and ocular moisture, helping to maintain tear secretion levels, improve tear film stability, and support the normal physiological state of the corneal epithelium, thereby relieving eye dryness and discomfort caused by environmental stimuli or increased eye strain. Animal experiments showed that after 14 days of continuous administration of the composition, experimental mice exhibited a significantly increased tear secretion volume compared to the control group without the composition, with an increase of approximately 165%. Corneal fluorescein staining scores were also significantly reduced, indicating improved ocular surface condition and significantly enhanced eye comfort.

The invention relates to the technical field of plant oil extraction, in particular to a preparation method of paeonia floribunda seed oil rich in active substances, which comprises the following steps: combining processes of pretreatment, squeezing treatment and refining treatment, and controlling the preheating temperature, heating stopping time and idle cooling process parameters in the squeezing treatment to obtain the paeonia floribunda seed oil rich in active substances. Physical squeezing is kept in a low-temperature environment, and heat-sensitive active substance components such as oleic acid, linoleic acid and alpha-linolenic acid can be reserved to the maximum extent. And in combination with refining treatment, the grease amount is reserved to the maximum extent, so that the oil yield is remarkably improved. Through the process combination of pretreatment, squeezing treatment and refining treatment, physical squeezing is kept in a low-temperature environment by controlling the preheating temperature, the heating stopping time and idling cooling process parameters in the squeezing treatment, and heat-sensitive active substance components such as oleic acid, linoleic acid and alpha-linolenic acid can be reserved to the maximum extent. And in combination with refining treatment, the grease amount is reserved to the maximum extent, so that the oil yield is remarkably improved.

The invention relates to the technical field of precise nutrition, and particularly discloses an alpha-linolenic acid personalized supplement judgment method and system based on fatty acid metabolism related genotypes. The method comprises the following steps: acquiring genotype information of at least one single nucleotide polymorphism site of an individual in a fatty acid desaturase gene cluster and / or a fatty acid elongase gene cluster; evaluating the expected metabolic response efficacy of the individual to alpha-linolenic acid supplementation; and judging whether alpha-linolenic acid is suitable for supplementing n-3 polyunsaturated fatty acid or not according to an evaluation result. The technical problem that an existing one-cut type alpha-linolenic acid supplement scheme is ineffective to people with low hereditary metabolism is solved, and precise nutrition intervention based on the genetic background is achieved.

The invention discloses a method for detecting the content of various components in a puffball sample and application of the method, and the method comprises the following steps: taking puffball sample powder, adding an extraction solvent for extraction, and preparing to obtain a test solution; taking alpha-linolenic acid, linoleic acid, palmitic acid, oleic acid, stearic acid and ergosterol reference substances, and adding a solvent to prepare a mixed reference substance solution; respectively injecting the mixed reference substance solution and the test solution into an ultra-high performance liquid chromatograph for detection, and comparing the maps of the reference substance solution and the test solution; and making a regression equation by using the sample size Ln value and the peak area Ln value of each component, and calculating the content of the components in the puffball sample. According to the quality detection method for the puffball sample, the contents of the six components can be simultaneously determined, the operation is simple, the accuracy is high, the recovery rate is reliable, the detection efficiency is high, and a basis is provided for standardizing the market of puffball medicinal materials and preparations and reasonably developing the puffball medicinal materials and preparations.

The present invention provides a composition for alleviating or preventing a symptom of allergic rhinitis or allergic conjunctivitis, the composition containing dihomo-γ-linolenic acid (DGLA) as an active ingredient.