321 results about "Linolenic acid" patented technology

A composition is formulated in a therapeutic amount to maintain and promote hair health and growth in a human in need thereof. The composition may comprise a shelf stable, supercritical CO2 fluid extracted, enhanced lipidosterolic extract of Serenoa repens (LSESr). The enhanced LSESr may have a ratio of free fatty acids to total fatty acids that is greater than about 83.0% and an enrichment of lauric, myristic, oleic and linoleic acids as free fatty acids to total free fatty acids that is about 81.0% or greater. An enrichment of linoleic and linolenic acids together may be greater than about 4.0%.

The invention discloses a serum-free culture medium for in-vitro proliferation of poultry muscle stem cells and application of the serum-free culture medium, and belongs to the technical field of cell engineering, a basic serum-free culture medium is DMEM-F12, a complete serum culture medium is a mixture of DMEM-F12 added with 10% of fetal calf serum and 1% of mycillin, and the volume ratio of a culture medium supplement to the basic culture medium is 1: 1000; the serum-free culture medium contains a supplement; the supplement comprises the following compositions: insulin, transferrin, fibroblast growth factor 2, epidermal growth factor, catalase, ethanolamine, vitamin B12, linoleic acid, linolenic acid, retinol acetate, tocopherol, L-alanine, L-proline, sodium selenite, sodium pyruvate, reduced glutathione, berberine hydrochloride and analogues thereof.

The invention relates to the field of extraction of sacha inchi oil, in particular to a preparation method and application of sacha inchi oil, and aims to solve the problems of large loss of heat-sensitive components, solvent residue and low oil yield in the traditional process. The ratio of cellulase to pectinase to xylanase to lipase is 3: 2: 1: 0.5, and carrying out enzymolysis for 2-3 hours at the pH value of 4.5-5.5 and the temperature of 45-50 DEG C; the obtained enzymolysis product is subjected to 28-40kHz ultrasonic treatment for 20-30 minutes, and then is subjected to two-stage gradient squeezing (pre-squeezing at 50-55 DEG C to final squeezing at 35-40 DEG C) in combination with 0.1 mu m ceramic membrane filtration, so that efficient oil extraction and active ingredient protection are realized. The oil yield of the sacha inchi oil is increased to 50.0%, the content of alpha-linolenic acid is larger than or equal to 42%, the content of vitamin E reaches 158 mg / 100 g, no solvent is left, and the sacha inchi oil is suitable for the fields of functional food and cosmetics.

The invention discloses a macrophage-polymer lipid nanoparticle composite drug delivery system, a preparation method thereof and application of the macrophage-polymer lipid nanoparticle composite drug delivery system in cryptococcus infection resistance. According to the drug delivery system, alpha-linolenic acid (ALA), high molecular weight chitosan oligosaccharide (HCOS) and amphotericin B (AmB) are used as raw materials to construct nanoparticles AmB-PLHNs, the nanoparticles are loaded in macrophages through the phagocytosis of the macrophages, and the AmB-PLHNs (at) M1 is constructed. AmB is a common medicine for clinically treating Cn infection, but has the problems of toxic and side effects on kidney and incapability of being transferred into the center. The AmB-PLHNs (at) M1 carries AmB-PLHNs to be transferred into the brain according to the blood-brain barrier penetrating characteristic of M1 type macrophages, and the brain entering efficiency of the medicine is improved. By combining the phagocytosis of macrophages on cryptococcus, the inflammation immune regulation function of the macrophages and the bacteriostatic action of the drug-loaded nanoparticles, the drug-loaded nano-particles are used for treating Cn central infection, and the toxic and side effects of AmB are reduced.

The invention relates to a bacteriostatic washing machine tank cleaning block composition and a preparation method thereof, and the bacteriostatic washing machine tank cleaning block composition comprises the following components: peroxide salt, a surfactant, a chelating agent, an acid substance, modified silicon dioxide, an active oxygen activator, a release agent, an adhesive, a filler and water; wherein the modified silicon dioxide is obtained by firstly hydroxylating silicon dioxide, then carrying out esterification reaction with hexadecenoic acid and linolenic acid, and finally polymerizing with sodium acrylate. The modified silicon dioxide can generate mechanical friction with stubborn dirt to promote the dirt to fall off, a high-crosslinking-degree net-shaped organic chain segment formed on the surface of the modified silicon dioxide has amphipathicity, after the modified silicon dioxide is compounded with the surfactant and other components, a synergistic effect is generated to improve the decontamination effect, the compatibility of all the components can be improved, and the cleaning effect is improved. Falling stubborn dirt can be wrapped, and the decontamination effect is further improved; all the components are compounded, so that a good antibacterial effect and the like are achieved, and a good prospect is achieved.

The invention discloses a preparation method of linolenic acid structural ester with synergistic biological activity, and relates to the technical field of linolenic acid structural ester preparation.The preparation method comprises the steps that specific lipase is prepared through rhizopus wei, and after an SBA-15 molecular sieve is treated, the lipase is fixed to prepare a composite catalyst; the preparation method comprises the following steps: after hydroxyl protection of quercetin, esterifying quercetin with EPA to obtain quercetin-EPA ester; esterifying glycerol and linolenic acid in the presence of a composite catalyst and microwaves, and purifying to obtain an intermediate product; carrying out microwave catalytic ester exchange on the intermediate product and quercetin-EPA ester in tert-butyl alcohol through a composite catalyst to obtain a crude product; and removing impurities from the crude product by a urea adduction method, and carrying out molecular distillation graded purification, column chromatography, deodorization and filtration to obtain a final product. According to the invention, linolenic acid, quercetin and EPA are combined through molecular design to form a compound structure ester with a multi-target effect, and the linolenic acid, quercetin and EPA achieve functional complementation through a synergistic effect, so that the biological activity of the compound structure ester is enhanced.

The invention discloses a preparation method of fermented peony seed oil. The fermentation process is optimized, different strains such as lactic acid bacteria, saccharomycetes and bifidobacteria are innovatively combined for multiple times of fermentation, and the lactic acid bacteria and saccharomycetes mixed strains are used for primary fermentation, so that components are preliminarily decomposed, and the viscosity is reduced; a bifidobacterium strain is used for secondary fermentation, so that the components are further optimized. After fermentation, supercritical CO2 extraction and freezing purification are performed to obtain the high-quality peony seed oil. According to the process, the content of monounsaturated fatty acid oleic acid in the peony seed oil is increased to 80.06%, the content of polyunsaturated fatty acid linoleic acid and linolenic acid is reduced, the product stability is improved, the skin feeling is fresh and cool when the peony seed oil is used, the permeation rate is increased, the DPPH free radical scavenging rate is increased, and the peony seed oil has an anti-eczema effect.

The invention belongs to the technical field of catalysts, and discloses a catalyst for preparing high-purity oleic acid as well as a preparation method and application of the catalyst. According to the catalyst for preparing the high-purity oleic acid, a SiO2 molecular sieve serves as a carrier, Pd and Pt serve as active components, and the molar ratio of Pd to Pt is 3: 2-4: 1. According to the invention, by controlling the carrier, the active component and respective dosages, the prepared catalyst can perform selective hydrogenation on vegetable oil containing linoleic acid and / or linolenic acid to obtain high-purity oleic acid.

The invention provides a preparation method and application of high alpha-linolenic acid, and relates to the technical field of medicines. Comprising the following steps: selecting purple perilla seeds, cleaning, drying, mechanically grinding in a protective atmosphere, and filtering to obtain purple perilla seed crude oil; carrying out degumming, deacidification, decoloration and dewaxing treatment on the crude perilla seed oil in a protective atmosphere to obtain primarily enriched crude alpha-linolenic acid oil; and respectively carrying out supercritical CO2 extraction, enzymatic molecular distillation and preparative chromatographic separation on the preliminarily enriched perilla seed oil to obtain the high alpha-linolenic acid. According to the method, purple perilla seeds are subjected to multi-stage treatment, extraction and refinement are performed layer by layer through three steps of supercritical CO2 extraction, enzymatic molecular distillation and preparative chromatographic separation, free fatty acid in purple perilla seed crude oil can be effectively removed, and alpha-linolenic acid is enriched to obtain high alpha-linolenic acid with the purity higher than 96%. The method is suitable for mass production in the medicine field.

The invention discloses a pharmaceutical composition for treating atopic dermatitis and a preparation method and application of the pharmaceutical composition for treating atopic dermatitis. The pharmaceutical composition comprises matrix vaseline, a humectant, a moistening agent, a repairing agent and an antioxidant. Two lipid components (gamma-linolenic acid and linoleic acid) are supplemented, so that eczema-like dermatitis can be obviously relieved, skin lesion inflammatory cell infiltration is reduced, the content of fatty acid in a skin barrier is recovered, treatment of atopic dermatitis is assisted, and the treatment effect on the atopic dermatitis is remarkable; the pharmaceutical composition disclosed by the invention has the advantages of quick effect on atopic dermatitis, small toxic and side effects, good safety and good medicinal prospect.

The invention discloses a method for promoting development of swallowing glands of bumblebees, the method is characterized in that the mass ratio of linoleic acid to alpha-linolenic acid in bumblebee feed is adjusted to be (1-5): 1, and the development of the swallowing glands of the bumblebees is promoted, the method is simple and easy to implement, and by adjusting the ratio of the linoleic acid to the alpha-linolenic acid in the bumblebee feed, the development of the swallowing glands of the bumblebees is promoted. According to the method, normal development of the giant bumblebees is guaranteed, diet preference of the giant bumblebees is met, nutrient fluctuation of natural pollen is made up, development of swallow glands can be remarkably promoted, feeding of larvae of the giant bumblebees is facilitated, and growth and development of offspring of a bee colony are facilitated.

This invention belongs to the field of genetic engineering technology, specifically involving GmDREB2A2 Application of genes in regulating fatty acid content in plants. Overexpression. GmDREB2A2 Gene regulation of fatty acid content in plants. This invention utilizes an exogenous gene sequence expression vector to express the coding sequence shown in SEQ ID NO.1. GmDREB2A2 Gene sequences are introduced into plants to enable GmDREB2A2 Gene overexpression. Under these conditions, the plant's linolenic acid, linoleic acid, and palmitic acid content were significantly higher than the control, while the content of eicosatrienoic acid and erucic acid was significantly reduced.

The invention discloses a composite material as well as a preparation method and application thereof, and relates to the technical field of high polymer materials. The composite material comprises the following components in parts by weight: 10-50 parts of epoxy resin; 10-20 parts of vegetable oleic acid; 1-3 parts of sulfur; 70-90 parts of a modified heat-conducting filler; the modified heat-conducting filler is obtained by modifying a heat-conducting filler through a double-bond-terminated silane coupling agent; the vegetable oleic acid comprises at least one of eleostearic acid, linolenic acid, linoleic acid, soybean oleic acid, oleic acid, ricinoleic acid and undecylenic acid. The composite material disclosed by the invention not only has the characteristics of high thermal conductivity and excellent electrical insulation performance, but also has good hot working performance and recycling capability, and shows a wide application prospect in the fields of high-thermal-conductivity green electronic packaging materials, electrical insulation coatings and sustainable composite materials.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.

The application provides a diglyceride oil containing alpha-linolenic acid for improving vascular health in six aspects and a preparation method and application, and relates to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing oil, glycerol and water, and then adding a lipase Lecitase Ultra to obtain a pretreated mixture after stirring; the mixed oil is a mixture of safflower seed oil, yuanbao maple seed oil, perilla seed oil, milk thistle seed oil and peony seed oil; a lipase Lipase DFA Amano 15 and a lipase Palatase 20000L are pretreated to obtain a fixed mixed lipase, which is mixed with the pretreated mixture for enzymolysis, and then subjected to molecular distillation. Through the specific ratio of edible oil and the specific enzymolysis process, the product not only improves the stability and taste, but also improves the vascular health in multiple aspects of reducing blood uric acid, reducing total blood cholesterol, reducing blood triglyceride, reducing blood sugar, reducing blood pressure, resisting inflammation, resisting oxidation and preventing arterial thrombosis.

The invention belongs to the technical field of mineral separation, and particularly relates to an environment-friendly composite flotation agent and application, and the composite flotation agent comprises the following raw materials in percentage by mass: 36.0%-37.5% of oleic acid, 8%-9% of linoleic acid, 1%-2.5% of linolenic acid, 20%-22% of a surfactant, 0.5%-0.7% of an inhibitor, 1%-2% of tributyl citrate and the balance of a solvent. The prepared composite flotation agent is beneficial to flotation separation of gangue minerals and tungsten ores, has a good flotation effect on low-grade tungsten ores, and is green and environmentally friendly.

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

The invention relates to the technical field of functional edible oil processing, and discloses zanthoxylum oil with an auxiliary anticancer function prepared on the basis of an aqueous enzymatic method, and the zanthoxylum oil is prepared from the following raw materials in parts by weight: 88-95 parts of zanthoxylum seed oil, 3-8 parts of high-purity oleoresin, 0.6 part of compound enzyme, 1-4 parts of volatile essential oil and 0.05 part of a stabilizer, the preparation method of the zanthoxylum oil with the auxiliary anticancer function based on the aqueous enzymatic method is characterized by specifically comprising the following steps: 1, mixing and adjusting the pH value: crushing zanthoxylum seeds, mixing the crushed zanthoxylum seeds with drinking water, and adjusting the pH value to 5.5; 2, enzymolysis: adding a compound enzyme, and carrying out enzymolysis at 50 DEG C for 4 hours; according to the present invention, through the specific raw material ratio and preparation steps, the low temperature enzymolysis process is adopted, the activity of alpha-linolenic acid and other heat-sensitive components is effectively retained, the composite enzymolysis cooperates with the steam distillation to achieve the efficient extraction and compounding of a variety of active components, and the anti-cancer function of the zanthoxylum oil is effectively improved.

Methods and compositions comprising free fatty acids for the treatment, including prevention, of viral infections, including the treatment of Acute Respiratory Distress Syndrome (ARDS). The compositions comprising free fatty acids, optionally linolenic acid, linoleic acid, and / or palmitic acid, may be used in methods for treating viral infections, optionally, coronavirus infections, for example, SARS-CoV, MERS-CoV, and / or SARS-CoV-2 / COVID-19, and influenza virus infections, optionally, H1N1 and / or H5N1.

This invention discloses a low-temperature reverse flotation collector for collophane and its preparation method, belonging to the field of collophane flotation technology. The low-temperature reverse flotation collector for collophane of this invention is prepared from the following raw materials: linoleic acid, linolenic acid, and gallic acid. This invention generates a low-temperature reverse flotation collector for collophane containing two OH groups and one COOH group through an esterification reaction of linoleic acid, linolenic acid, and gallic acid. In acidic pulp, the flotation recovery rate is 20-50% lower than before the esterification reaction, and the dosage is significantly improved, resulting in a substantial increase in concentrate P2O5 grade and recovery rate. Furthermore, the low-temperature reverse flotation collector for collophane can be used for flotation at a low temperature of 5-10℃. The flotation foam generated during the flotation process has a short duration, low foam stability, and is easily defoamed, which is beneficial for continuous operation and tailings recovery.

The invention discloses a strawberry FaLEC2 gene and application thereof, and relates to the field of plant molecular biology, the nucleotide sequence of the FaLEC2 gene is as shown in SEQ ID NO.1, and the amino acid sequence of protein encoded by the strawberry FaLEC2 gene is as shown in SEQ ID NO.2. The invention studies the application of the FaLEC2 gene in regulating and controlling the contents of vinblastine, 3-deoxy-D-mann-2-octanone acid, raspberry glycoside and linolenic acid in strawberries. It is found that through overexpression of the FaLEC2 gene, the content of vinblastine, the content of 3-deoxy-D-mann-2-octanone acid, the content of raspberry glycoside and the content of linolenic acid in the strawberries can be increased; the metabolism regulation and control mechanism of the vinblastine, the 3-deoxy-D-mann-2-octanone acid, the raspberry glycoside and the linolenic acid in the strawberry fruits can be further known, so that the content of the vinblastine, the 3-deoxy-D-mann-2-octanone acid, the raspberry glycoside and the linolenic acid in the strawberry fruits can be regulated and controlled by utilizing a biotechnological means; an important foundation is laid for strawberry variety improvement.

The invention discloses China-hemp peptide multi-nutrient protein powder with a metabolism improving function, which comprises China-hemp seeds, soybean protein isolate, China-hemp seed protein, chickpea protein, pea protein, China-hemp peptide, rice protein, broad bean protein, mung bean protein and wheat hydrolyzed protein. Wherein the Chinese hemp seeds are 15-25 parts, the soybean protein isolate is 10-20 parts, the Chinese hemp seed protein is 5-15 parts, the chickpea protein is 8-12 parts, the pea protein is 8-12 parts, the Chinese hemp peptide is 8-15 parts, the rice protein is 5-10 parts, the broad bean protein is 5-10 parts, the mung bean protein is 5-10 parts, and the like. According to the present invention, besides the protein nutrition, the small molecule peptide and 10 other nutrients such as Omega3, gamma-linolenic acid, vitamin E and a variety of minerals are contained, such that the complete protein nutrition is provided, and the health-care food is rich in Omega-3 fatty acid, gamma-linolenic acid, vitamin E, potassium, magnesium, zinc and other minerals with different molecular magnitudes extracted from China-hemp seeds, the ten nutrients, a plurality of plant proteins and small molecule peptides have a synergistic effect to jointly form a basis for improving the metabolic function.

The invention discloses an application of fat powder as a chicken feed raw material in replacing corn and improving fatty acid composition of eggs. The fat powder accounts for 2-6% of the feed; the dosage ratio of unsaturated fatty acid to saturated fatty acid is 2.7-3.83; in the fat powder, the saturated fatty acid accounts for 70-135 mg / g; 270 to 365 parts of unsaturated fatty acid; the unsaturated fatty acid comprises 1.03 to 2.56 parts of palmitoleic acid; 90.8 to 124 parts of oleic acid; 164 to 216 parts of linoleic acid; 0.819 to 1 part of eicosaenoic acid; 10 to 15.9 parts of alpha-linolenic acid; ePA (ethylene propylene diene monomer) is 1.49 to 3.24; and DHA (docosahexaenoic acid) 1.62- By adopting the scheme of finely blending the fat powder to replace corn and improve the fatty acid composition of eggs, the egg quality of laying hens can be obviously improved, and the feed cost is obviously improved.

The invention relates to the technical field of edible oil production, and specifically discloses a preparation method and preparation equipment of blended oil fatty acid, comprising a barrel, a motor, a shaft, two stirring components, a feed pipe and a discharge pipe, wherein the feed pipe and the discharge pipe are both connected to the barrel, the motor is fixedly connected to the barrel and is located above the barrel, one end of the shaft is fixedly connected to the output end of the motor, the other end of the shaft is inserted into the interior of the barrel, and the two stirring components are symmetrically arranged on both sides of the shaft; the preparation equipment of blended oil fatty acid has better use effect, the prepared blended oil fatty acid can meet the production requirements, and it is ensured that linoleic acid and linolenic acid in the blended oil will not be unbalanced.

A perilla oil α-linolenic acid microcapsule and its preparation method belong to the food processing field, including the following steps: (1) extraction of perilla oil; using a bio-enzyme-assisted low-temperature pressing method, a compound enzyme is added to perilla seeds for enzymatic hydrolysis, followed by low-temperature pressing to collect perilla seed oil; (2) enrichment and purification of α-linolenic acid; the perilla seed oil obtained in step (1) is purified by molecular distillation technology, and the perilla oil fraction enriched with α-linolenic acid is collected; (3) microencapsulation; using the perilla oil enriched with α-linolenic acid obtained in step (2) as the core material, after selecting a suitable composite wall material, perilla oil α-linolenic acid microcapsules are obtained by spray drying. This invention improves the extraction efficiency of perilla oil, retains the natural activity of α-linolenic acid; efficiently enriches α-linolenic acid through molecular distillation technology, increasing the content of functional active ingredients in the product; and improves the oxidative stability and storage performance of α-linolenic acid.

The invention discloses a selenium-rich camellia oil capsule capable of effectively improving a health-care effect and a preparation method thereof. The selenium-rich camellia oil capsule contains unsaturated fatty acid, saturated fatty acid, tea polyphenol, camellia saponin, squalene, 14-methylpentadecanoic acid, linoleic acid, stearic acid, linolenic acid, protein, cellulose, vitamin E, thiamine, riboflavin, nicotinic acid, magnesium, calcium, iron, zinc, copper, manganese, potassium, phosphorus and selenium. According to the technical scheme, the quality and the health-care effect of the camellia oil can be effectively improved, meanwhile, the loss of active ingredients is reduced, the oil yield is increased, and the nutritional ingredients of the camellia oil are kept. Besides, the method also has the advantage of environmental protection, as supercritical CO2 can be recycled, the influence on the environment is reduced, the selenium-enriched camellia oil capsule can stay in the intestinal tract of a human body for a long time, the absorption rate of effective components is improved, and unsaponifiable matters, such as gamma-tocopherol, beta-sitosterol and squalene, of the camellia oil are retained.

The present invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a locus qFAC.A9-1 significantly associated with the fatty acid composition of rapeseed seeds and its application. <h2 style=";text-align:left;direction:ltr">qFAC.A9‑1 Its peak SNP marker Bn‑A09‑p3047090 is located at the 2,966,603th base of chromosome A09 of the rapeseed Darmor V4.1 reference genome, which can explain an average of 12.0% of the phenotypic variance. The PARMS marker designed using this SNP was used to detect rapeseed germplasm resources, and it was found that it is easy to operate and has clear typing. The average arachidonate, erucic acid and linolenic acid content of seeds of AA genotype materials is lower than that of GG genotype materials, while the content of linoleic acid, oleic acid, palmitic acid and stearic acid is higher, which has a good selection effect on the fatty acid composition of rapeseed seeds.

The invention provides an extraction method of high-content alpha-linolenic acid, and relates to the technical field of biological extraction. The extraction method of the high-content alpha-linolenic acid comprises the following steps: pretreating raw materials, destroying a plant cell wall structure through related operations of crushing and degreasing, releasing grease components, improving the subsequent extraction efficiency, removing phospholipid and free fat, and avoiding impurities influencing the purification effect; the method comprises the following steps: carrying out directional hydrolysis on lipase, cutting off sn-1 and 3 site ester bonds by utilizing lipase specificity, converting triglyceride into free fatty acid, reserving the structural integrity of sn-2 site alpha-linolenic acid, and enabling free alpha-linolenic acid to be more easily separated through subsequent distillation / chromatography compared with glyceride form. Through a silver ion complexing chromatography technology, alpha-linolenic acid and other unsaturated fatty acids with similar structures can be accurately separated, the purity of the final product stably reaches 98% or above, the pharmaceutical grade application standard is met, and the technical bottleneck that a traditional purification method is difficult to break through the purity of 90% is solved.