97 results about "Linolenic acid" patented technology

This invention belongs to the field of genetic engineering technology, specifically involving GmDREB2A2 Application of genes in regulating fatty acid content in plants. Overexpression. GmDREB2A2 Gene regulation of fatty acid content in plants. This invention utilizes an exogenous gene sequence expression vector to express the coding sequence shown in SEQ ID NO.1. GmDREB2A2 Gene sequences are introduced into plants to enable GmDREB2A2 Gene overexpression. Under these conditions, the plant's linolenic acid, linoleic acid, and palmitic acid content were significantly higher than the control, while the content of eicosatrienoic acid and erucic acid was significantly reduced.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

A perilla oil α-linolenic acid microcapsule and its preparation method belong to the food processing field, including the following steps: (1) extraction of perilla oil; using a bio-enzyme-assisted low-temperature pressing method, a compound enzyme is added to perilla seeds for enzymatic hydrolysis, followed by low-temperature pressing to collect perilla seed oil; (2) enrichment and purification of α-linolenic acid; the perilla seed oil obtained in step (1) is purified by molecular distillation technology, and the perilla oil fraction enriched with α-linolenic acid is collected; (3) microencapsulation; using the perilla oil enriched with α-linolenic acid obtained in step (2) as the core material, after selecting a suitable composite wall material, perilla oil α-linolenic acid microcapsules are obtained by spray drying. This invention improves the extraction efficiency of perilla oil, retains the natural activity of α-linolenic acid; efficiently enriches α-linolenic acid through molecular distillation technology, increasing the content of functional active ingredients in the product; and improves the oxidative stability and storage performance of α-linolenic acid.

The invention provides an extraction method of high-content alpha-linolenic acid, and relates to the technical field of biological extraction. The extraction method of the high-content alpha-linolenic acid comprises the following steps: pretreating raw materials, destroying a plant cell wall structure through related operations of crushing and degreasing, releasing grease components, improving the subsequent extraction efficiency, removing phospholipid and free fat, and avoiding impurities influencing the purification effect; the method comprises the following steps: carrying out directional hydrolysis on lipase, cutting off sn-1 and 3 site ester bonds by utilizing lipase specificity, converting triglyceride into free fatty acid, reserving the structural integrity of sn-2 site alpha-linolenic acid, and enabling free alpha-linolenic acid to be more easily separated through subsequent distillation / chromatography compared with glyceride form. Through a silver ion complexing chromatography technology, alpha-linolenic acid and other unsaturated fatty acids with similar structures can be accurately separated, the purity of the final product stably reaches 98% or above, the pharmaceutical grade application standard is met, and the technical bottleneck that a traditional purification method is difficult to break through the purity of 90% is solved.

The invention discloses a cell culture medium supplement as well as a preparation method and application thereof, and relates to the technical field of cell biology. The supplement comprises DL-alpha-tocopheryl acetate, linolenic acid, ethanolamine, L-carnitine hydrochloride, 3, 3 ', 5-triiodoL-sodium thyrosinate, and serum albumin. As a cell culture additive with definite chemical components and simple composition, the cell culture additive can replace a traditional culture system with unknown chemical components or too complex composition, and is used for supporting proliferation, differentiation and characterization analysis of cells. The culture medium additive is clear in chemical component, simple to prepare, stable in effect and relatively low in cost, can promote in-vitro proliferation and oxidation resistance of cells including human embryonic stem cells and mesenchymal stem cells, and has important application value in actual production.

A heart boosting formulation comprises: L-carnitine, alpha-linolenic acid, citrulline, vitamin D3, lycopene, holy basil, cinnamon and beetroot.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The invention discloses a composition for comprehensive conditioning of an internal environment, which comprises the following components in parts by weight: 1000-1200 parts of alpha-linolenic acid, 100-300 parts of cordyceps militaris powder, 50-150 parts of oyster powder, 50-150 parts of ginseng powder, 50-150 parts of pueraria peptide, 50-150 parts of fresh lalang grass rhizome powder and 50-150 parts of polygonatum odoratum powder. Wherein the purity of the alpha-linolenic acid is not lower than 80%. According to the invention, high-purity alpha-linolenic acid and six specific medicinal and edible components are scientifically proportioned to construct a multi-level synergistic system, and all the components complement and synergism on a plurality of targets such as lipid lowering, circulation promoting, sugar stabilizing, acid discharging, repairing and the like, so that comprehensive conditioning on disorder of multiple indexes such as blood fat, blood sugar, uric acid, blood pressure and the like is realized; the limitation of a single component or a simple compound product is broken through, and the comprehensive synergistic conditioning of the internal environment is realized; all the components are selected from a medicinal and edible substance catalog, so that the traditional Chinese medicine composition is safe and mild, meets the physique requirements of Chinese people on warm nourishing and conditioning, gives consideration to conditioning and repairing, and is high in adaptability.

The invention discloses a water-soluble fertilizer for promoting swelling and coloring of crop fruits. The water-soluble fertilizer is prepared from the following raw materials: 30 to 50 parts of herba portulacae fermentation liquor, 15 to 25 parts of potassium nitrate, 10 to 20 parts of monopotassium phosphate, 2 to 8 parts of chelated trace elements, 2 to 4 parts of polyglutamic acid, 1 to 3 parts of compound sodium nitrophenolate and 1 to 3 parts of betaine. The invention belongs to the technical field of agricultural fertilizers, and particularly provides a water-soluble fertilizer for promoting fruit swelling and coloring of crops, which is characterized in that inert precursor substances in purslane are converted into efficient active components by a fermentation technology, and alpha-linolenic acid derivatives generated by fermentation of the purslane stimulate expression of relaxation proteins in fruit cell walls to achieve a fruit swelling effect.

This invention relates to ketogenic nutritional powder containing medium- and long-chain fatty acid triglycerides, its preparation method, and its application. It belongs to the field of food science and engineering technology. It uses enzymatically synthesized medium- and long-chain fatty acid triglyceride structured lipids as the sole fat source, which is different from the traditional physical mixture of long-chain triglycerides and medium- and long-chain oils. Medium-chain fatty acids and long-chain fatty acids are simultaneously bound to the same glycerol molecule. Fatty acid digestion and absorption are synchronized and metabolized, which can give full play to the synergistic nutritional advantages. It retains the characteristics of medium-chain fatty acids that provide rapid energy and are not easy to accumulate in body fat, while also supplementing essential fatty acids such as linoleic acid and α-linolenic acid. At the same time, it overcomes the disadvantages of pure medium-chain fatty acid oils that cause great gastrointestinal irritation and have a low smoke point.

The invention discloses animal and plant blend oil conforming to a dietary fatty acid equilibrium standard and a preparation process thereof, and belongs to the technical field of edible oil and fat processing. The blend oil is prepared from the following components in parts by mass: 5 to 30 parts of low-cholesterol animal oil, 70 to 95 parts of various vegetable oil and 0.001 to 0.015 part of an emulsifier RXLY-2. The method is characterized in that the fatty acid composition of the final product simultaneously meets the condition that the mass ratio of saturated fatty acid to monounsaturated fatty acid to polyunsaturated fatty acid is (0.95-1.05): (0.95-1.05): (0.95-1.05), and the mass ratio of linoleic acid to alpha-linolenic acid is 4: 1-6: 1. The preparation process comprises the following steps: preheating and emulsifying the animal oil, mixing with the vegetable oil, heating and curing, filtering and filling and the like. According to the invention, through scientific proportioning and a specific process, the internationally recognized'double gold proportion 'of dietary fatty acids is accurately realized, the problems of unbalanced nutrition and imbalance ratio of essential fatty acids of single edible oil are solved, and the product has good stability and is suitable for long-term eating by various people.

This invention discloses a blueberry anthocyanin nutritional solid composition for relieving eye discomfort. The composition comprises blueberry anthocyanin solid powder, algal oil powder, zinc gluconate, and selenium-enriched yeast, and may optionally contain lipid nutrients containing gamma-linolenic acid, as well as food additives and excipients. Oral administration of this composition provides the body with various nutritional supports related to ocular surface function and ocular moisture, helping to maintain tear secretion levels, improve tear film stability, and support the normal physiological state of the corneal epithelium, thereby relieving eye dryness and discomfort caused by environmental stimuli or increased eye strain. Animal experiments showed that after 14 days of continuous administration of the composition, experimental mice exhibited a significantly increased tear secretion volume compared to the control group without the composition, with an increase of approximately 165%. Corneal fluorescein staining scores were also significantly reduced, indicating improved ocular surface condition and significantly enhanced eye comfort.

The invention relates to the technical field of plant oil extraction, in particular to a preparation method of paeonia floribunda seed oil rich in active substances, which comprises the following steps: combining processes of pretreatment, squeezing treatment and refining treatment, and controlling the preheating temperature, heating stopping time and idle cooling process parameters in the squeezing treatment to obtain the paeonia floribunda seed oil rich in active substances. Physical squeezing is kept in a low-temperature environment, and heat-sensitive active substance components such as oleic acid, linoleic acid and alpha-linolenic acid can be reserved to the maximum extent. And in combination with refining treatment, the grease amount is reserved to the maximum extent, so that the oil yield is remarkably improved. Through the process combination of pretreatment, squeezing treatment and refining treatment, physical squeezing is kept in a low-temperature environment by controlling the preheating temperature, the heating stopping time and idling cooling process parameters in the squeezing treatment, and heat-sensitive active substance components such as oleic acid, linoleic acid and alpha-linolenic acid can be reserved to the maximum extent. And in combination with refining treatment, the grease amount is reserved to the maximum extent, so that the oil yield is remarkably improved.

The invention relates to the technical field of precise nutrition, and particularly discloses an alpha-linolenic acid personalized supplement judgment method and system based on fatty acid metabolism related genotypes. The method comprises the following steps: acquiring genotype information of at least one single nucleotide polymorphism site of an individual in a fatty acid desaturase gene cluster and / or a fatty acid elongase gene cluster; evaluating the expected metabolic response efficacy of the individual to alpha-linolenic acid supplementation; and judging whether alpha-linolenic acid is suitable for supplementing n-3 polyunsaturated fatty acid or not according to an evaluation result. The technical problem that an existing one-cut type alpha-linolenic acid supplement scheme is ineffective to people with low hereditary metabolism is solved, and precise nutrition intervention based on the genetic background is achieved.

The present invention provides a composition for alleviating or preventing a symptom of allergic rhinitis or allergic conjunctivitis, the composition containing dihomo-γ-linolenic acid (DGLA) as an active ingredient.

The invention discloses a dual-mode cooperative detection method for unsaturated fatty acid in Baijiu, and relates to the technical field of food detection. According to the method, aiming at the characteristic of a high alcohol ester matrix of the white spirit and the detection pain point that unsaturated fatty acid is easy to oxidize and large in content span, selective enrichment and matrix purification are performed on a white spirit sample through a silanization modified C18 solid-phase extraction small column, and then low-temperature anti-oxidation derivatization is completed through matching of a composite derivatization reagent and an anti-oxidation system. Then, the palmitic acid, the oleic acid and the linoleic acid are quantified by adopting a gas chromatography-mass spectrometry method, meanwhile, the alpha-linolenic acid is directly detected by adopting a high performance liquid chromatography-quadrupole time-of-flight mass spectrometry method, and finally, results are calibrated and output by combining an internal standard method and an external standard method. According to the method, efficient purification of the Baijiu matrix is achieved, the oxidation loss rate of alpha-linolenic acid is controlled within 3%, high-precision quantification of constant components and high-sensitivity detection of trace components can be synchronously achieved, the method is suitable for detection of full-flavor and full-alcoholic-degree Baijiu, stability and universality are excellent, and reliable technical support is provided for Baijiu quality evaluation.

Isotopically modified polyunsaturated lipids containing a 1, 4-diene system are provided.SOLUTION: Wherein R and R ' comprise residues of linolenic, arachidonic, eicosapentaenoic, or docosahexaenoic acids, or esters thereof, wherein the esters, if present, are C1 - C21 alkyl esters, wherein the ruthenium complex further comprises a cyclopentadiene (Cp) ligand, and wherein the ruthenium in the ruthenium complex is in the + 1 oxidation state (Ru + 1).SELECTED DRAWING: None

The invention discloses fatty acid desaturase for heterologous synthesis of alpha-linolenic acid and application of the fatty acid desaturase, and belongs to the technical field of biology. The purpose of the invention is to improve the yield and conversion rate of alpha-linolenic acid. The invention provides a fatty acid desaturase. The amino acid sequence of the fatty acid desaturase is as shown in SEQ ID NO. 4 or SEQ ID NO. 5. And a theoretical basis is provided for improving the yield of alpha-linolenic acid.

Described herein are pet food compositions and methods for using them. Such compositions may comprise soluble fiber and certain ratios of linolenic acid to fatty acids. The methods may include feeding the pet an effective amount of the pet food composition. The methods may include increasing the lean mass of a companion animal comprising feeding an effective amount of a pet food composition comprising soluble fiber and certain ratios of linolenic acid to fatty acids.

The application discloses an alpha-linolenic acid and metal iridium coupling compound, a preparation method and application thereof, and relates to the field of medicine, in particular to an alpha-linolenic acid and metal iridium coupling compound, which has good antitumor activity on human cervical cancer cells, human hepatoma cells and mouse breast cancer cells. The preparation method is simple and easy to operate, and the product has good antitumor activity.

The present disclosure relates to topical skin care compositions that increase the hydration of aging skin, improve the barrier function of aging skin, provide antioxidant protection to aging skin and enhance dermal-epidermal junction health in aging skin. In particular, the disclosure relates to a topical composition comprising (i) one or more collagens; (ii) one or more barrier support agents, preferably linoleic acid and / or linolenic acid; (iii) one or more bio-hydration support agents, preferably a botanical 5a reductase inhibitor comprising butyl avocadate, saw palmetto extract, and / or black pepper extract; (iv) one or more dermal-epidermal junction agents, preferably comprising passiflora edulis seed oil; (v) one or more antioxidants, preferably comprising nelumbo nucifera germ extract and / or vitis vinifera (grape) flower extract; and (vi) hyaluronic acid, hyaluronic acid derivatives or combinations of hyaluronic acid and / or hyaluronic acid derivatives.

A composition is provided comprising an effective amount of a combination of gamma linolenic acid (GLA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA). The composition comprises about 5% w / w to about 25% w / w GLA, and about 5% w / w to about 25% w / w of a mixture of DHA and EPA. Methods of modulating fatty acid profiles or treating an inflammatory condition in a horse include administering an effective amount of the composition to the horse. The inflammatory conditions capable of being treated include gastric ulcers, airway inflammation, joint inflammation, or combinations thereof.

The invention discloses MLCT grease for improving the stability of an ice cream milk base and a preparation method of the MLCT grease. The MLCT grease is prepared by directionally hydrolyzing grease containing conjugated linolenic acid and then directionally esterifying medium-chain fatty acid and palmitic acid at sn-1 and sn-3 positions. In the CLnA-enriched sn-2 MLCT structure ester constructed by the invention, sn-2 site fatty acid is mainly CLnA and accounts for 13-15% of the total amount of fatty acid, medium-chain fatty acid accounts for more than 30% of the total amount of fatty acid, and the content of MLCT accounts for more than 93% of the total amount of fatty acid. The CLnA-enriched sn-2 MLCT structure ester prepared by the invention is added into a basic formula of ice cream, so that the bleeding rate of the ice cream can be reduced by 10% or more, the expansion rate can be increased by 8% or more, and the melting rate is also improved.

The invention provides a chitosan-based compact dressing, a preparation method and a water-light disintegrating tablet. Relates to the field of skin care. The dressing comprises the following raw materials: aloe liquid, a chitosan care solution and a traditional Chinese medicine extract, the mass ratio of the aloe liquid to the chitosan care solution to the traditional Chinese medicine extract is 10: 3: 1, and the dressing formed according to the scheme has a very good effect of tightening the private parts of women in an experiment, micromolecular hyaluronic acid, collagen peptide, nicotinamide, gamma-linolenic acid, asiaticoside, trehalose, allantoin, chitosan and linarin have a very good tightening effect, an optimal use ratio of the materials is obtained through experimental verification, and the synergistic effect of the materials in the ratio is the strongest; and the effect of tightening and shrinking the female vagina can be better achieved.

The invention discloses a plasma metabolism biomarker combination for predicting and intervening propofol-induced respiratory depression, a detection method and application, and relates to the technical field of biological science. According to the method, non-target metabonomics detection is conducted on preoperative plasma of 298 subjects through UPLC-MS, 14-metabolite panels are obtained through machine learning supervised feature screening, a diagnosis model is established through verification of discovery and verification queues, and the prediction AUC of severe respiratory depression reaches 0.86-0.91 (discovery queue) and 0.86-0.91 (verification queue). Mouse in-vivo function verification proves that the key metabolite alpha-linolenic acid obviously improves the respiratory rate, the tidal volume and the minute ventilation volume under two administration paradigms of pretreatment and treatment of a mouse propofol-RD model, shortens the recovery time of righting reflex, has a good safety foundation, and has a good application prospect. Prompt can be converted into a foresight supplement or rapid treatment medication thought in a perioperative period, and a'prediction and intervention 'integrated clinical value is formed.

The present invention discloses a pharmaceutical composition having activity of anti-tumor comprising a therapeutically effective amount of one or more compounds of formula I as the active ingredient, conventional pharmaceutically acceptable carriers, and one or more pharmaceutically acceptable acidic components, wherein said acidic component is selected from a group consisting of oleic acid, stearic acid, linolenic acid, fumaric acid, benzoic acid, tartaric acid, sorbic acid, lactic acid, citric acid, acetic acid and EDTA, wherein X is 0 or S; R is substituted or unsubstituted C3˜C10 straight or branched alkyl, C3˜C10 cycloalkyl, C3˜C10 straight or branched alkenyl or C3˜C10 straight or branched chain alkynyl; and if R has substituting group, said substituting group may be C1˜C6 alkyl, C1˜C6 alkoxy, C1˜C6 alkylthio group, C1˜C6 alkyl amino group, phenyl or phenyl substituted by halogen. Further, said composition may be made into all sorts of conventional dosage forms, in particular the oral dosage forms and topically administered transdermal dosage form. In addition, the invention discloses a method to use the composition for treatment of tumor.

The application belongs to the technical field of food processing, and particularly relates to a preparation method of a coating agent for reducing food allergenicity, the coating agent and application. The preparation method comprises the following steps: a certain amount of alpha-linolenic acid is weighed according to a proportion, and is dissolved in preheated ethanol to form an alpha-linolenic acid-ethanol solution; a certain amount of rosemary extract is weighed according to a proportion, and is dissolved into an aqueous solution to prepare a rosemary extract aqueous solution through ultrasonic dissolution; carrageenan and sodium carboxymethyl cellulose are added into the rosemary extract aqueous solution to obtain a mixture aqueous solution; the alpha-linolenic acid-ethanol solution is added into the mixture aqueous solution and uniformly stirred to obtain the coating agent for reducing food allergenicity. The application first prepares a composite coating agent rich in lipid compounds by using lipids and representative food gums, and the composite coating agent is applied to low-allergenic food, especially whole-grain products of peanuts and nuts, so that the allergenicity of the food can be effectively reduced, and the nutritional value of the food can be improved.