27 results about "Palmitoleic acid" patented technology

The application provides a recombinant Yarrowia lipolytica capable of producing palmitoleic acid at a high yield, a construction method and application thereof, and belongs to the technical field of bioengineering. Yarrowia lipolytica The recombinant Yarrowia lipolytica is obtained by knocking out peroxisome biogenesis factor 10 and triacylglycerol lipase 4 in the genome of Yarrowia lipolytica and inserting an acetyl-CoA carboxylase, a Δ9 desaturase, an acetyl-CoA diacylglycerol acyltransferase, a glycerol-3-phosphate acyltransferase, a lysophosphatidylcholine acyltransferase and a phosphatidylcholine:diacylglycerol acyltransferase gene expression cassette. Experiment proves that the recombinant Yarrowia lipolytica can efficiently ferment and produce palmitoleic acid, and realizes efficient synthesis of the natural product palmitoleic acid from plants in Yarrowia lipolytica.

The application belongs to the technical field of bio-based packaging materials, and particularly relates to a novel double-layer composite film and application thereof. The novel double-layer composite film comprises the following steps: reacting palmitic anhydride and carboxymethyl cellulose to obtain carboxymethyl cellulose palmitate; adding a pH-sensitive natural dye indicator to obtain carboxymethyl cellulose palmitate nanoemulsion containing a pH-sensitive substance; then loading a natural polymer material to prepare a film material; finally adding the film material to the carboxymethyl cellulose palmitate to prepare a composite film, thereby obtaining the novel double-layer composite film. The double-layer composite film can be self-emulsified without adding a surfactant, and has the ability to load functional indicator (pH-sensitive color indicator) substances; meanwhile, the barrier performance of the film material, such as a chitosan film, to water vapor and oxygen is improved, and the double-layer composite film has a very promising application in food or fruit preservation. The method provided by the application is not only green in raw materials, but also uses a flow casting method, which can be applied to industrial production and has a good application prospect.

The invention belongs to the technical field of agricultural pest control, and particularly relates to a novel drosophila melanogaster attractant and a preparation method and application thereof. The invention discloses a novel drosophila melanogaster attractant which comprises the following components in percentage by mass: 10%-20% of brown sugar, 1%-5% of acetic acid, 1%-5% of ethanol, 1%-5% of phenethyl alcohol, 0.5%-2.0% of oleic acid, 0.1%-1% of palmitoleic acid, 0.1%-0.5% of sodium benzoate, 0.2%-0.5% of ascorbic acid, 0.2%-0.3% of sodium carboxymethyl cellulose, 0.5%-1.0% of sodium dodecyl benzene sulfonate and the balance of water. Field test results show that the novel drosophila melanogaster attractant is high in trapping rate of drosophila melanogaster on fruit crops, the used raw materials are low in price, the preparation process is simple, the dosage form is a liquid dosage form, field application is facilitated, the novel drosophila melanogaster attractant can be widely applied to prevention and control of field drosophila melanogaster, and the market prospect is good.

This application discloses a corrosion and scale inhibitor and its preparation method. On one hand, this application provides a corrosion and scale inhibitor, wherein the mass ratio of each component of the corrosion and scale inhibitor includes: 60-70 parts of palmitoleic acid-acrylic acid-lignin sulfonic acid copolymer and 30-40 parts of maleic acid-acrylic acid-EDTA copolymer. On the other hand, this application provides a method for preparing the above-mentioned corrosion and scale inhibitor, including the following steps: S1, preparing palmitoleic acid-acrylic acid-lignin sulfonic acid copolymer; S2, preparing maleic acid-acrylic acid-EDTA copolymer; S3, weighing each component according to the formula, mixing thoroughly, and obtaining the corrosion and scale inhibitor. The application exhibits good environmental friendliness, excellent scale inhibition effect against calcium and magnesium ions, good dispersibility, and excellent corrosion inhibition effect.

The purpose of this invention is to provide an oil-in-water emulsion and a foamable oil-in-water emulsion with minimized thickening and solidification during refrigerated storage.　This oil-in-water emulsion contains oil / fat having a weight ratio S2Po / S2M of 0.03 or more. S2Po is a triglyceride in which two Ss and one Po are bonded, and S2M is a triglyceride in which two Ss and one M are bonded. Here, S denotes a 16-22 C saturated fatty acid, M denotes a 16-22 C monovalent unsaturated fatty acid, and Po denotes a palmitoleic acid (C16: 1 (n-7)).

The application belongs to the technical field of microbial fermentation, and discloses a method for improving the production of palm oleic acid in Schizochytrium sp. by fermentation regulation and application. In the fermentation process of Schizochytrium sp., one or more combined regulation strategies of controlling the fermentation temperature, optimizing the carbon-nitrogen ratio of the fermentation medium and adjusting the pH value of the fermentation liquid are adopted to change the metabolic flow direction, so as to improve the accumulation of palm oleic acid C16:1 in oil. Compared with genetic engineering modification, the method does not need complex genetic operation and has high safety. Through verification of a 5L fermentation tank, the proportion of palm oleic acid in oil can be significantly increased from less than 1% in conventional fermentation to 16.12%, and the oil yield is stable, so that the method has good industrial application potential.

The invention discloses a composite protective agent, a preparation method of active dry yeast, the active dry yeast and application, and belongs to the technical field of bioengineering and fermented food. The composite protective agent comprises a first-phase biological enhancer and a second-phase film-forming protective agent, the first-phase biological enhancer comprises palmitoleic acid and trehalose; the second-phase film-forming protective agent comprises trehalose; the first-phase biological enhancer is added when the yeast grows into the logarithmic growth later stage or the residual sugar of the fermentation liquor is reduced to 40-60% of the residual sugar of the initial fermentation liquor; and the second-phase film-forming protective agent is added before drying. The active dry yeast protective agent is applied to the aspect of active dry yeast preparation, the problem that an existing active dry yeast protective agent for the beer industry cannot prevent leakage of intracellular substances (such as K < + > and nucleotide) is solved, the ultra-high viable bacterium rate of 95% or above can be achieved, and the foam stability and ester flavor of beer are remarkably improved.

The purpose of the present invention is to provide an oil-and-fat composition which is a non-trans acid and a non-lauric acid suitable for use in various oily food products. This oil-and-fat composition satisfies all of the following. · The content of stearic acid in the constituent fatty acid composition is 3-40 mass%. · The content mass ratio of stearic acid with respect to palmitic acid in the constituent fatty acid composition is 0.09-5.0. · The content of S3 is 1-15 mass%. · The content mass ratio of SSU with respect to S2U is 0.3 or more. · The content mass ratio of S2Po with respect to S2U is 0.01 or more. Here, S represents a saturated fatty acid having 16 carbon atoms or more, U represents an unsaturated fatty acid having 16 carbon atoms or more, Po represents palmitoleic acid, S2U represents a triglyceride in which 2 molecules of S is bonded to 1 molecule of U, S3 represents a triglyceride in which 3 molecules of S are bonded, S2Po represents a triglyceride in which 2 molecules of S are bonded to 1 molecule of Po, and SSU is an asymmetric triglyceride in which S is bonded to position 2, and S and U are bonded to positions 1 and 3.

The invention relates to the technical field of microorganisms, and discloses a pre-culture solution for improving the survival rate of saccharomyces boulardii under in-vitro simulated gastrointestinal stress conditions and application of the pre-culture solution. Each liter of the pre-culture solution contains the following components: 8-12 g of yeast extract, 18-22 g of peptone, 18-22 g of glucose, 3-7 g of characteristic casein, 0.01 [mu] g of palmitoleic acid and the balance of water, and the pH value of the pre-culture solution is 5.8-6.2; the characteristic casein is casein coated with astaxanthin. According to the method disclosed by the invention, the saccharomyces boulardii is inoculated into the pre-culture solution of the characteristic casein and palmitoleic acid which are externally supplemented and coated with the astaxanthin for pre-culture, so that the saccharomyces boulardii can keep a relatively high survival rate under the action of stress factors such as low pH value of gastrointestinal tracts, cholate and digestive enzymes; a suitable growth environment can be provided for saccharomyces boulardii, and growth and reproduction of the saccharomyces boulardii are promoted, so that the biomass is increased, and the survival rate of the saccharomyces boulardii is increased.

The invention discloses application of palmitoleic acid in preparation of a medicine for protecting nerves and treating cerebral ischemia-reperfusion injury, and belongs to the field of biological medicine. Animal experiments prove the neuroprotective effect of palmitoleic acid on cerebral ischemia-reperfusion injury for the first time. By establishing a mouse cerebral ischemia reperfusion injury model, the palmitoleic acid is found to be capable of remarkably improving neurological impairment and reducing the volume and area of cerebral infarction, which indicates that the palmitoleic acid can be used for effectively limiting ischemic necrosis of brain tissues. According to the application, the research limitation that palmitoleic acid is traditionally applied to metabolic diseases is broken through, a brand new treatment strategy is provided for solving clinical ischemic stroke reperfusion injury, a solid foundation is laid for developing novel neuroprotective drugs, and important clinical application value and development prospects are shown in the aspect of improving prognosis of stroke patients.

The purpose of the present invention is to address the problem of providing an oil and fat composition which is free of trans acid and lauric acid, and is suitable for use in various oily foods. An oil and fat composition according to the present invention satisfies all of the following conditions: ･ In a constituent fatty acid composition, the content of palmitic acid is 30-70 mass% ･ In the constituent fatty acid composition, the content of unsaturated fatty acid is 20-60 mass% and the content of S3 is 1-15 mass% ･ The content mass ratio of SSU to S2U is 0.5 or more ･ The content mass ratio of S2Po to S2U is 0.01 or more S represents a saturated fatty acid having 16 or more carbon atoms, U represents an unsaturated fatty acid having 16 or more carbon atoms, Po represents palmitoleic acid, S2U represents a triglyceride in which two S molecules and one U molecule are bonded, S3 represents a triglyceride in which three S molecules are bonded, S2Po represents a triglyceride in which two S molecules and one Po molecule are bonded, and SSU represents an asymmetric triglyceride in which S is bonded at the 2-position, and S and U are bonded at the 1- and 3-positions.

The application discloses a kind of compound insect oil and its application in cardiovascular and cerebrovascular repair, the compound insect oil is composed of palmitoleic acid-enriched pentatomidae oil and olive oil, wherein the content of palmitoleic acid in palmitoleic acid-enriched pentatomidae oil is ≥20g / 100g.The application obtains pentatomidae crude oil by supercritical CO2 extraction, then directional enrichment is carried out by low-temperature distillation and molecular rectification, the content of palmitoleic acid is increased to more than 20g / 100g, then the enriched pentatomidae oil is compounded with olive oil, and the compound insect oil is prepared by low-temperature homogenization emulsification, deodorization, sterilization process.The compound insect oil prepared by the application can significantly reduce the levels of TC, TG and LDL-C, while significantly increasing the level of HDL-C, showing excellent hypolipidemic effect.In addition, the compound insect oil can also significantly reduce the level of endothelin-1 and increase the level of nitric oxide, effectively improve the function of vascular endothelium, and can be used for the prevention and adjuvant therapy of cardiovascular and cerebrovascular diseases, with good application prospect.

The invention relates to the cross technical field of biotechnology and daily chemicals, and particularly discloses a natural plant composition for removing odors of old people, which comprises ligusticum root extract, eupatorium extract, tea extract, rosemary leaf extract, nigella nigra seed oil, tea tree oil, polygonum cuspidatum root extract and agastache rugosus extract. Through the synergistic effect of all the components, the composition inhibits release of palmitoleic acid from the upstream, performs anti-oxidation and antibacterial intervention in the midstream, decomposes odor molecules from the downstream, assists in repairing the skin microenvironment, forms an intervention network covering the whole path of generation of the old-age odor, can efficiently remove the old-age odor, and has a good application prospect in related odor removal products.

The invention relates to the technical field of mineral flotation, in particular to a phosphorite reverse flotation low-temperature collecting agent and a preparation method thereof.The phosphorite reverse flotation low-temperature collecting agent is composed of fatty acid sodium, fatty alcohol polyoxyethylene ether sodium sulfate, palmitoleic acid, an auxiliary anionic surfactant, petroleum sulfonic acid and palm fatty acid light components according to a specific proportion. The preparation method comprises the following steps: heating, melting and mixing the oil-phase components to obtain an oil-phase mixture, dissolving the water-phase surfactant in hot water to obtain a water-phase solution, slowly adding the oil phase into the water phase at a specific stirring rate, and then carrying out high-speed shearing emulsification and cooling to obtain a final product. By microcosmically constructing a stable composite micelle structure, the problems that a conventional collecting agent is poor in dispersity and prone to crystallization at the low temperature are effectively solved.

Dedust compositions suitable for use as dedust oil for mineral wool applications comprise a) from 95 percent by weight to 99.99 percent by weight of a triacyl glyceride sourced from a plant or animal oil, the triacyl glyceride having an IV of from 45 to 70, a viscosity at 30 C of from 30 mPa·s to 80 mPa·s, and a Solid Fat Content of 15% w / w or less at 10 C; and b) from 100 ppm to 2,500 ppm of an antioxidant. The dedust composition exhibits a flash point of at least 280 C. In aspects, the dedust composition is liquid at 23 C. In aspects, the triacyl glyceride is palm super olein.

This invention relates to the field of molecular biology, specifically to SNP molecular markers associated with palmitoleic acid trait in sheep muscle and their applications. Based on the detection of exons in the sheep SMAD1 gene, this invention identifies an SNP site at position 493 of exon 2 of the SMAD1 gene or position 13,440,579 of chromosome 17 of sheep. The base at this site is either A or G, and allele A is positively correlated with high palmitoleic acid trait in muscle. Furthermore, a method for detecting this SNP molecular marker is provided to achieve rapid and accurate genotyping of the SMAD1 gene in sheep populations (e.g., double-dried meat sheep). Moreover, based on the significant correlation between this site and individual palmitoleic acid and fatty acid content, SNP markers can be used to screen sheep individuals with higher palmitoleic acid content at an early stage, thereby improving the speed of breeding superior sheep.

The application relates to the technical field of molecular detection, and particularly discloses the use, chip, method and application of a molecular marker for beef cattle meat trait detection, wherein the molecular marker comprises 62 SNP sites; the physical positions of the 62 SNP sites are determined based on whole genome sequence alignment of a Hereford cattle reference genome, the version number of the whole genome sequence of the Hereford cattle reference genome is GCF_002263795.3; and the 62 SNP sites are shown in Table 1. The molecular marker of the application can be applied to detection of growth traits and meat quality traits of different breeds of beef cattle, wherein the growth traits include body weight, body height, cross section height, body slant length, chest circumference and pipe circumference; and the meat quality traits include backfat thickness, eye muscle area, protein, intramuscular fat content, amino acid, myristic acid, palmitoleic acid, linoleic acid and arachidonic acid.

Disclosed in the present invention is a composition for alleviating geriatric body odor using a Lysimachia davurica extract which removes 2-nonenal, which is a substance that causes geriatric body odor, and inhibits the production of 2-nonenal from palmitoleic acid.

The application provides a weather-resistant polyester FDY oil agent and a preparation method thereof. The weather-resistant polyester FDY oil agent comprises the following components in parts by weight: 58-62 parts of a composite smoothing agent, 16-20 parts of an emulsifier, 5-9 parts of an antistatic agent, 3-7 parts of a penetrating agent, and 6-10 parts of deionized water, wherein the composite smoothing agent is composed of glyceryl palmitoleate, polyglyceryl-6 oleate and pentaerythritol-4EO-triisostearate at a mass ratio of 30-40:10-15:8-12. The emulsion stability of the treating agent at low temperature can be significantly improved by reasonably compounding glyceryl palmitoleate, polyglyceryl-6 oleate and pentaerythritol-4EO-triisostearate, and the prepared oil agent has good heat resistance and can ensure the stability of the production process.

The invention discloses a crude oil asphaltene solid inhibitor and a preparation method and application thereof, the preparation method comprises the following steps: reacting palmitoleic acid, aluminum oxide and diethylenetriamine, then reacting with ethidene diamine under the action of ethanol, and dissolving the obtained reactant and clay in water to obtain a mixture; and adding vinyl acetate into the mixture, uniformly stirring, adding cyclohexane, vinyl alcohol polyether and an initiator, uniformly mixing, and carrying out polymerization reaction to obtain the crude oil asphaltene solid inhibitor. The prepared crude oil asphaltene solid inhibitor can reduce the crude oil asphaltene precipitation temperature by 7-13 DEG C, and still has a good asphaltene precipitation inhibition function under the condition that the use concentration is reduced.

The invention discloses a novel application of palmitoleic acid and a culture solution for promoting in-vitro development and maturation of porcine oocytes, and belongs to the field of reproductive biology in developmental biology. The method can be used for preparing products for promoting in-vitro development and maturation of oocytes. According to the present invention, pig cumulus-oocyte complexes (COCs) are cultured under the in vitro condition, and the palmitoleic acid is added to the culture solution to explore the influence of the palmitoleic acid on the first polar body discharge rate, the cortical particle distribution, the mitochondrial distribution and the oocyte reactive oxygen species (ROS) level of the oocytes, such that the results show that the in vitro addition of the palmitoleic acid can improve the oocyte quality, and the in vitro addition of the palmitoleic acid can provide the research on the oocyte first polar body discharge rate, the cortical particle distribution, the mitochondrial distribution and the oocyte reactive oxygen species (ROS) level; and a good effect is achieved.

This invention discloses a longevity biomarker based on serum metabolomics and its application, belonging to the field of biomedical technology. The longevity biomarker disclosed in this invention, based on serum metabolomics, includes mandelic acid, glutethimide, fenmetrazine, hydroxyheptanoic acid, norfloxacin, N-acetylserotonin, 3,4-methylenedioxyphenylamine, palmitoleic acid, cinconidine, gulonose, 5-aminopentanal, 12,13-dihydroxy-9Z-octadecenoic acid, anserine, (3Z)-phytochrome bile, phytocyanin, and L-selenomethylselenocysteine. This invention provides new biomarkers for diagnosing healthy aging and predicting human lifespan, and can be used to prepare products that predict the likelihood of longevity, showing promising applications in the field of anti-aging and health.

The invention belongs to the technical field of bioengineering and microbial fermentation, and discloses high-yield palmitoleic acid engineering schizochytrium limacinum as well as a construction method and application of the high-yield palmitoleic acid engineering schizochytrium limacinum. The heterologous delta9 desaturase gene is subjected to gene source screening, promoter optimization and target gene copy number optimization, so that the capability of converting C16: 0 into C16: 1 is enhanced, and the content of palmitoleic acid in schizochytrium limacinum grease is remarkably increased. According to the engineering strain disclosed by the invention, the palmitoleic acid content can be obviously increased, preferably, the palmitoleic acid content can reach 4% or above, and further, the palmitoleic acid content can reach 6% or above. The method has the advantages of clear technical route and strong operability, and can provide engineering strains and application schemes for industrial production of high palmitoleic acid functional lipid raw materials.

The invention belongs to the technical field of microbial fermentation, and discloses a method for increasing the yield of schizochytrium limacinum palmitoleic acid through fermentation regulation and application, in the fermentation process of schizochytrium limacinum, one or more combined regulation strategies of controlling the fermentation temperature, optimizing the carbon nitrogen ratio of a fermentation culture medium and adjusting the pH value of fermentation liquor are adopted, and the yield of the schizochytrium limacinum palmitoleic acid is increased. And the metabolism flow direction of thalli is changed, so that the accumulation of palmitoleic acid C16: 1 in grease is improved. Compared with genetic engineering modification, the method disclosed by the invention does not need complex genetic manipulation and is high in safety; a 5L fermentation tank verifies that the proportion of palmitoleic acid in the grease can be remarkably increased to 16.12% from less than 1% of conventional fermentation, the grease yield is stable, and the method has good industrial application potential.

The present invention relates to a fatty acid composition comprising (A) a fatty acid having 16 carbon atoms and a cis double bond at the 9-position and (B) a fatty acid having 16 carbon atoms and a trans double bond at the 9-position, wherein the content ratio [(A):(B)] of component (A) to component (B) ranges from 99.99:0.01 to 60:40 by mass.　According to the present invention, a fatty acid composition is provided which contains palmitoleic acid in a high content, exhibiting antibacterial activity, and has excellent storage stability.

The invention discloses a mild and efficient makeup removal wet tissue and a preparation method thereof, and belongs to the technical field of cosmetics. The cleansing oil is used for solving the technical problems that in the prior art, a conventional surfactant adopted by the cleansing oil is poor in oil phase component emulsifying capacity, and an adopted wet tissue material is poor in cleansing emulsion loading capacity. The preparation method of the mild and efficient makeup removal wet tissue comprises the following steps: S1, uniformly mixing an oil phase system, a water phase system, a composite surfactant and a high polymer carrier to obtain makeup removal emulsion; s2, the spinning base cloth is soaked in the makeup removing emulsion, and the mild and efficient makeup removing wet tissue is prepared. Glucose is modified by glycine and then reacts with methyl cocoate to synthesize the composite surfactant. Glucan and palmitoleic acid are subjected to an esterification reaction, and a high polymer carrier is prepared. The prepared mild and efficient makeup removal wet tissue has the advantages that the emulsification efficiency of a water phase component and an oil phase component is high, and the load rate of the wet tissue material to makeup removal emulsion is high.