125 results about "Milk fat" patented technology

The invention discloses an FABP3 gene SNPs marker for detecting sheep milk production traits and application of the FABP3 gene SNPs marker, and belongs to the technical field of biology. According to the invention, lactating sheep is taken as a research object, Sanger and other technologies are applied to detect a nucleotide sequence variation site of an FABP3 gene, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep lactation traits is researched, and a molecular genetic marker for regulating and controlling the sheep lactation traits is excavated; on the SNP1 site, the cream-fat percentages of AG and GG genotype individuals are respectively 0.99% and 1.01% higher than those of AA type individuals. On the site SNP2, the milk fat rates of TC and TT genotype individuals are 1.18% and 1.23% higher than those of CC type individuals respectively, and the daily lactation volumes of the TC and TT genotype individuals are 1.97% and 1.27% higher than those of the CC type individuals respectively.

The invention relates to a nutritional composition and application thereof. The nutritional composition comprises medium-chain and long-chain fatty acid triglyceride and a milk fat globule film, wherein the weight ratio of the medium-chain and long-chain fatty acid triglyceride to the milk fat globule film is 0.1: 1 to 50: 1. The nutritional composition disclosed by the invention can play a role in obviously improving sleep.

The invention discloses an SNP molecular marker for increasing the milk yield of Holstein cattle and application of the SNP molecular marker, the SNP molecular marker is located at a mutation site of a Holstein cattle genome Leptin gene, the gene number of the Leptin gene in an NCBI database is NC037333.1, the SNP molecular marker is located at the 1761th basic group of a cDNA sequence of the Leptin gene, and the mutation basic group is A or G. When the basic group of the SNP molecular marker is A, the genotype is AA or AG, and the milk fat rate and the milk protein rate of Holstein cow milk production are high; when the basic group of the SNP molecular marker is G, the genotype is GG, the milk yield of Holstein cattle in 305 days is high, and the milk quality is not obviously reduced. The marker is applied to genetic improvement of dairy cows, and the frequency of dominant alleles can be improved generation by generation by selecting individuals carrying the dominant alleles, so that the lactation performance of the dairy cows is effectively improved, the breeding efficiency is optimized, and the competitive advantage in the field of dairy cow breeding is enhanced.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.

The present invention discloses a targeted knockout bovine FGFR1 The application of the sgRNA of the gene in constructing a bovine mammary epithelial cell line, wherein the sgRNA nucleotide sequence is shown in SEQ ID NO: 1. The mammary epithelial cell line obtained by constructing the sgRNA of the present invention through CRISPR / Cas9 technology, FGFR1 Protein expression was significantly downregulated, and knockout FGFR1 The gene expression had no significant effect on the viability and proliferation of mammary epithelial cells. PCNA 、 Cyclin D1 、 Cyclin E1 There was no significant change in the mRNA expression of the genes related to milk protein synthesis (such as CSN1S1 、 CSN2 、 CSN3 、 EFL5 ), lactose synthesis-related genes ( GLUT1 and HKⅡ ) and milk fat synthesis-related genes ( SCD‑1 ) were significantly decreased, providing a basis for in-depth analysis FGFR1 It provides key tools for the regulatory mechanisms of mammary epithelial cell proliferation, lactation synthesis and other biological behaviors.

The invention belongs to the field of functional feeds, and discloses a preparation method of a coarse cereal feed for sheep, which comprises the following steps: crushing one or two coarse cereals of buckwheat, broom corn millet, millet and sorghum, and mixing the crushed coarse cereals with raw materials such as enzymolysis soybean meal and alfalfa meal; adding a compound bacteria solution containing lactobacillus plantarum, lactobacillus acidophilus and saccharomyces boulardii for anaerobic fermentation; the nano zinc oxide and the yucca extract are further strengthened to improve the oxidation resistance and ammonia reduction capacity of the feed; and finally, granulating and spraying a sodium alginate-chitosan coating. Through coarse cereal combination optimization, soybean meal enzymolysis pretreatment and composite flora fermentation synergistic effect, the feed protein utilization rate, the fiber degradation rate and the animal production performance are remarkably improved. Experiments show that the milk fat content of the saanen milk goats fed with the feed is increased by 15-23%, the daily gain of the Alba cashmere goats is increased by 25-35%, the feed conversion ratio is optimized to 11.2-12.3: 1, and compared with the prior art, the feed has outstanding nutrition balance and application pertinence.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

A portable method and device for monitoring sodium ions in breast milk, belonging to the field of lactation status feedback, is disclosed. The method is executed after a breast milk container is installed on the main unit and includes the following steps: breast milk container identification and batch parameter loading, posture detection and gating, dry self-calibration of the electrical channel, thermal pulse temperature response acquisition and thermodynamic virtual volume feature extraction, low-frequency / high-frequency electrical response acquisition and bubble interference feature extraction, optical scattering signal acquisition and fat / turbidity proxy feature extraction, and geometric boundary compensation, temperature compensation, milk fat shielding compensation, and sodium ion concentration inversion based on the multi-dimensional features. It can also perform nursing guidance push, result storage, and edge-cloud collaborative updates. The device includes a main unit and a breast milk container. The main unit contains a heating film, a temperature probe, an optical turbidity detection module, and an electrode detection module connected to a four-electrode structure. This solution can improve the accuracy, stability, and repeatability of breast milk sodium ion detection in a home setting.

The invention relates to the technical field of dairy product processing, in particular to a double-fat double-protein synergistic dairy product and a preparation method thereof. According to the preparation method, a ''whey protein (WP)-casein phosphopeptide (CPP)'' double-protein system and a ''milk fat (MF)-medium chain triglyceride (MCT)'' double-fat system are constructed, and a four-step synergistic process of ''pretreatment, composite emulsification, gradient assembly and low-temperature curing'' is adopted, so that stable combination of a chemical interface of protein and fat is realized, high-temperature stability breakthrough is realized, and the preparation method has the advantages of high-temperature stability, high-temperature stability and high-temperature stability. Meanwhile, high-pressure processing tolerance is improved, fat globule flocculation caused by high pressure is avoided, and long-term storage stability, texture and taste are guaranteed.

The present invention provides a method of feeding a ruminant animal, preferably a pregnant ruminant animal, using a phytogenic composition. By feeding the composition pre-partum, the colostrum yield may be increased and colostrum quality is improved. Moreover, milk yield and milk fat percentage may be increased during lactation phase of the ruminant animal.

The invention belongs to the technical field of fat synthesis, and particularly discloses a method for synthesizing 1, 3-dilinoleic acid-2-palmitic acid triglyceride, which comprises the following steps: S1, dissolving palm stearate in a solvent, and purifying to obtain PPP; s2, mixing the PPP purified in the step S1 with LA, dissolving, and adding immobilized lipase ANL-MARE to carry out primary reaction; after the primary reaction is completed, carrying out acid removal; and then adding LA again to carry out secondary reaction, filtering to remove enzyme after the reaction is completed, and separating and purifying to obtain the LPL structure lipid. According to the method, PPP-rich palm stearin and linoleic acid are used as raw materials, and immobilized lipase is utilized to catalyze two-step acidolysis reaction in a solvent-free system to synthesize the structured triglyceride rich in LPL. Compared with a common one-step acidolysis method, the method has the advantages that the relative content of LPL is increased by 10.27%, the relative content of sn-2PA is kept unchanged, the usage amount of enzyme is effectively reduced, and the LPL efficiently synthesized through enzymatic catalysis of the method has the potential of being used for production of human milk fat substitutes.

The utility model relates to the technical field of whey separation, in particular to a fat and whey separator in milk, which is characterized in that when the fat and whey separator is used, milk is stirred by a stirring component, milk fat particles are more uniformly distributed in the milk by stirring, subsequent separation is facilitated, and then a liquid conveying component is started; therefore, the stirred milk is filtered through one station of the double-station filtering assembly and cleaned through the other station, continuous separation of fat and whey of the milk is kept while filtering and cleaning are achieved, and the separation efficiency is improved; comprising a bottom plate and a disc type centrifugal machine, the disc type centrifugal machine is installed at the top end of the bottom plate, the device further comprises a stirring assembly, a liquid conveying assembly and a double-station filtering assembly, the liquid conveying assembly and the stirring assembly are arranged at the top end of the bottom plate, and the input end of the liquid conveying assembly communicates with the output end of the stirring assembly; the output end of the liquid conveying assembly communicates with the input end of the double-station filtering assembly, and the output end of the double-station filtering assembly communicates with the input end of the disc type centrifugal machine.

The invention provides a rumen bypass folic acid additive, a preparation method and application thereof and a method for producing milk rich in folic acid, and particularly belongs to the technical field of rumen bypass additives. The rumen bypass folic acid additive comprises folic acid, cobalamin, silicon dioxide, lipase, palm fat, coconut oil and calcium stearate. The rumen bypass folic acid additive can reduce folic acid degradation by rumen microorganisms, can directly reach small intestines to be absorbed, improves the folic acid utilization rate of ruminants, specifically can improve the milk yield, milk protein, milk fat and folic acid content and yield of dairy cows, and can produce folic acid-rich cow milk; the feed can also increase the plasma glucose level and reduce the concentration of beta-hydroxybutyric acid, so that the energy metabolism state of the dairy cow is improved, the production performance of the dairy cow is improved, and economic benefits are brought to pastures.

The invention relates to the field of molecular biology, and discloses a haplotype marker for dairy cow milk production butter-fat percentage character breeding and application of the haplotype marker, the haplotype marker is composed of three SNP sites in a strong linkage imbalance state, and the three SNP sites are located in a Block1 haplotype block region of a 10 # chromosome NC037337.1 of a dairy cow ARS-UCD1.2 genome; the three SNP sites are as follows: SNP1: g.59680573 A > C, SNP2: g.59743173 G > C, and SNP3: g.59743414 C > T; the dominant haplotype of the haplotype marker is H2, and the allele combination of the haplotype marker is SNP1-A, SNP2-G and SNP3-C; the homozygous H2H2 of the dominant haplotype H2 is significantly related to the high milk fat percentage character of milk production of dairy cows. The bottleneck of the traditional technology is solved, and the breeding accuracy is remarkably improved; the screening detection technical scheme is stable and reliable and has a wide application range; the milk production quality of groups is improved, and the industrial economic value is enhanced.

The present application relates to a kind of fat powder for reducing weaning piglet dead rate and its preparation method and application, and the composite lipid in the preparation raw material of fat powder includes 1,3-di-oleic acid-2-palmitic acid triglyceride, 24 degrees palm oil, rice bran oil, coconut oil, linseed oil, caprylic triglyceride, capric acid triglyceride, alpha-lauric acid monoglyceride and tributyrin.The present application develops the fat acid composition close to pig milk fat structure, let piglet continue lactation period in weaning period to enable approach mainly with lipid, meet the compound energy demand of fast, medium and slow, avoid falling into energy potential difference;And the specific combination of medium and short chain fatty acids, both can improve the energy efficiency of weaning piglet, also have bacteriostatic disease characteristics, can improve the immunity of piglet, reduce the diarrhea rate.This composite lipid overcomes the problem such as piglet energy deficiency and indigestion caused by single raw material oil due to unsuitable lipid structure, can significantly reduce weaning stress, reduce the diarrhea rate and dead rate.

The present invention belongs to the fields of molecular biotechnology and molecular marker technology, and specifically relates to an SNP locus related to yak milk density and milk fat content and its application. The SNP locus is located at the 13,729,718th position of chromosome 5 of the yak reference genome Bosgu_v3.0 version with the Ensemble accession number ENSBGRG00000003042, and the mutated base is C or T. The present invention also provides the application of a reagent for detecting the above SNP locus in the auxiliary breeding for detecting yak milk density and milk fat content. This molecular marker can be applied to the auxiliary selection of yak milk density and milk fat content traits, and the detection method is rapid and accurate; by screening the genotypes of this SNP molecular marker, the accuracy and effectiveness of the selection of yak milk density and milk fat content traits can be accelerated, and the economic benefits of yak breeding can be improved.

The invention relates to the technical field of food, in particular to mascarpone cheese and a preparation method thereof, and the mascarpone cheese comprises the following raw materials in percentage by mass: 70-85% of milk fat, 5-15% of milk solid substance, 0.2-2% of compound stabilizer, 0.1-1% of acidity regulator and the balance of water, the mascarpone cheese disclosed by the invention adopts a whey-free process, the 100% utilization rate of the raw materials is realized, no waste treatment burden exists, the process is simple, the mascarpone cheese can be directly produced on a reproduced cheese production line, the production efficiency is improved, manpower, material resources and equipment modification cost are saved, and the mascarpone cheese is free of preservatives and long in shelf life through UHT sterilization and sterile hot filling. The stability in a long shelf life period is realized by compounding a stable system, and a finished product is rich in milk flavor, smooth and fine in texture and moderate in hardness, and shows good application performance when being used for preparing desserts such as tiramisu and the like.

The present invention relates to a ruminant feed rich in particles of rumen labile components. The present invention relates to a granular ruminant feed in the form of granules or subsequently comminuted form, comprising a mixture of at least one solid granulated feed component and at least one rumen-labile component (added to the mixture). The invention also relates to a method for preparing the ruminant feed of said granules and to the use thereof in a method for modifying the milk fat concentration.

A frying oil with butter flavor, comprising a vegetable oil and hydrolyzed milk fat and optional anhydrous milk fat. Also disclosed is a method of preparing the frying oil and the use of the frying oil.

The invention relates to a milk fat replacer suitable for use in a chocolate or chocolate-like product, wherein the milk fat replacer comprises triglycerides, having the ability to mimic the functionality of milk fat in terms of its effects when used in chocolate or chocolate-like products. Further, the invention relates to a product comprising said milk fat replacer in addition to various use of said milk fat replacer.

The invention discloses a molecular marker for detecting the milk production character of cattle and an identification method, and belongs to the technical field of biology. The SNP A provided by the invention is g.8044024C / T, the SNP B provided by the invention is g.8081101 A / G, and the SNP C provided by the invention is g.8081205C / G. The molecular marker g.8044024C / T, g.8081101 A / G or / and g.8081205C / G is associated with the milk yield, the milk fat amount and the milk protein amount at a remarkable or extremely remarkable level (P = 0.02-2.00 E-16). By utilizing the SNP provided by the invention, early screening can be realized in the dairy cow breeding process, the breeding cost can be reduced, and the SNP has an important application prospect in dairy cow breeding.

Fat blend comprising (i) 20-65 wt % of a vegetable lipid source and (ii) 35-80 wt % of a milk fat composition comprising at least one bovine milk fat fraction, wherein the milk fat composition comprises, relative to total fatty acid moieties, 37-48 mol % of long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0) and wherein 28-43 mol % of all fatty acid moieties positioned on the sn-1,3 position of the glycerol backbone in said milk fat composition are long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0).

This invention discloses the application of Cordyceps sinensis mycelium fermentation culture in improving the immunity and milk quality of dairy cows, belonging to the field of animal husbandry technology. The Cordyceps sinensis mycelium fermentation culture is obtained by liquid fermentation of Cordyceps sinensis, followed by solid-liquid separation, collection of the liquid fraction, concentration, and spray drying. This invention applies the Cordyceps sinensis mycelium fermentation culture as a feed additive to lactating dairy cows, significantly improving key milk quality indicators, including milk fat percentage, milk protein percentage, lactose percentage, non-fat solids content, and milk somatic cell count. It also enhances serum immune indicators in lactating dairy cows, improving their immunity. This invention provides a novel and effective solution to the industry challenge of finding green and safe feed additives in animal husbandry, and has clear industrialization prospects.

The invention belongs to the technical field of experimental animal nutriology, and discloses a high-fat feed composition for mouse metabolic syndrome and a preparation process of the high-fat feed composition. The high-fat feed composition comprises casein VTF, DL-methionine, corn starch, maltodextrin, cane sugar, cellulose, anhydrous milk fat, corn oil, ethoxyquin, a mineral mixture S10001AIN-76A, calcium carbonate, a vitamin mixture 40060-D and cholesterol. The preparation process comprises the following steps: S1, pretreating the raw materials; S2, preparing the materials; S3, mixing the materials; S4, granulating; the high-fat feed provided by the invention can effectively improve the food consumption of mice and ensure the weight growth rate of the mice; and on the basis that the atherosclerosis animal model is stable, the time required for preparing the atherosclerosis mouse is shortened.

To provide: sherbet which sufficiently gives a lactic acid bacterium beverage flavor and has a good texture; and frozen dessert obtained with the sherbet which has both a rich feel due to milk fat and the lactic acid bacterium beverage flavor.SOLUTION: The present invention is sherbet having a lactic acid bacterium beverage flavor which contains glucomannan and has a milk solid content of less than 10 mass%. The sherbet preferably contains lactic acid bacterium fermented liquid. The sherbet preferably contains konjak powder as the glucomannan. The present invention is frozen dessert having the lactic acid bacterium beverage flavor which has one or more ice cream parts and one or more sherbet parts. The ice cream part has a milk solid content of 15.0 mass% or more and a milk fat content of 8.0 mass% or more. The sherbet part contains glucomannan and has the lactic acid bacterium beverage flavor.SELECTED DRAWING: Figure 1

The invention provides milk fat rich in concentrated chain triglyceride as well as a preparation method and application of the milk fat. The method for preparing the milk fat rich in the concentrated chain triglyceride comprises the following steps: (1) completely melting anhydrous milk fat in a water bath, preserving heat in the water bath, growing crystal at the temperature of 20-30 DEG C, and carrying out crystal separation to obtain soft fat and stearin; and (2) carrying out single-stage short-path molecular distillation on the molten soft fat at 175-185 DEG C to obtain a heavy-phase component and a light-phase component, and the light-phase component is the milk fat rich in the concentrated-chain triglyceride. The method is simple to operate and low in cost, and the MCT-rich soft fat is obtained through crystal growing separation and then is subjected to short-path molecular distillation, so that MCT can be effectively enriched, and the flavor quality of the milk fat is improved.

The present application relates to the technical field of livestock breeding, and discloses a feeding method for increasing milk fat rate of lactating donkey. The feeding method for increasing milk fat rate of lactating donkey comprises the following steps: feeding lactating donkey with a special feeding stuff for increasing milk fat rate of lactating donkey with specific nutrient level and nutrient substance ratio; wherein the nutrient level of the feeding stuff for increasing milk fat rate of lactating donkey is: 12±0.5 MJ / kg of digestible energy, 120±6 g / kg of crude protein, and 60±5 g / kg of crude fat content; and the nutrient substance ratio is: (50-60):(40-50) of crude protein ratio, and 2.2±0.2 of NDF / NFC. The feeding method for increasing milk fat rate of lactating donkey provided by the present application can promote the synthesis of milk fat of lactating donkey and increase the milk fat rate of lactating donkey by combining the increase of lipid and NDF in the feeding stuff under the condition of appropriate protein level of the feeding stuff.

This invention relates to a dairy processing technology and dairy products based on continuous hydraulic cavitation. The invention provides a dairy processing technology based on continuous hydraulic cavitation, comprising the following steps: (1) feeding the emulsion into a hydraulic cavitation reactor for continuous hydraulic cavitation pretreatment; (2) cooling the pretreated emulsion to remove excess milk fat, completing the defatting process; (3) ultrafiltration membrane concentration: concentrating the defatted emulsion through an ultrafiltration membrane to obtain lactose-free skimmed milk; (4) drying the lactose-free skimmed milk. This invention uses hydraulic cavitation technology to pretreat milk raw materials, completing the defatting and sterilization steps, and achieving a reduction in the average particle size of protein molecules, lactose, and ash content under low-temperature conditions, solving the problems of complex processing procedures, low product yield, and unsatisfactory product quality in traditional production technologies.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

The invention belongs to the field of gene editing, and particularly relates to a method for regulating milk fat content. The expression of genes in fatty acid and cholesterol synthesis pathways of the livers of the animals in the lactation period is specifically regulated and controlled through a gene delivery technology, the expression of liver apolipoprotein B (ApoB) and the triglyceride and total cholesterol level in blood can be remarkably reduced, then the lipid content of milk is effectively reduced, meanwhile, the food intake of the animals is reduced, and the production cost is reduced. And the milk protein content is not influenced. According to the method, milk fat components are accurately controlled through maternal liver gene editing for the first time, a new strategy is provided for breeding healthy dairy animals, and the method has wide application prospects.