100 results about "Milk fat" patented technology

The invention discloses an SNP molecular marker for increasing the milk yield of Holstein cattle and application of the SNP molecular marker, the SNP molecular marker is located at a mutation site of a Holstein cattle genome Leptin gene, the gene number of the Leptin gene in an NCBI database is NC037333.1, the SNP molecular marker is located at the 1761th basic group of a cDNA sequence of the Leptin gene, and the mutation basic group is A or G. When the basic group of the SNP molecular marker is A, the genotype is AA or AG, and the milk fat rate and the milk protein rate of Holstein cow milk production are high; when the basic group of the SNP molecular marker is G, the genotype is GG, the milk yield of Holstein cattle in 305 days is high, and the milk quality is not obviously reduced. The marker is applied to genetic improvement of dairy cows, and the frequency of dominant alleles can be improved generation by generation by selecting individuals carrying the dominant alleles, so that the lactation performance of the dairy cows is effectively improved, the breeding efficiency is optimized, and the competitive advantage in the field of dairy cow breeding is enhanced.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.

The present invention discloses a targeted knockout bovine FGFR1 The application of the sgRNA of the gene in constructing a bovine mammary epithelial cell line, wherein the sgRNA nucleotide sequence is shown in SEQ ID NO: 1. The mammary epithelial cell line obtained by constructing the sgRNA of the present invention through CRISPR / Cas9 technology, FGFR1 Protein expression was significantly downregulated, and knockout FGFR1 The gene expression had no significant effect on the viability and proliferation of mammary epithelial cells. PCNA 、 Cyclin D1 、 Cyclin E1 There was no significant change in the mRNA expression of the genes related to milk protein synthesis (such as CSN1S1 、 CSN2 、 CSN3 、 EFL5 ), lactose synthesis-related genes ( GLUT1 and HKⅡ ) and milk fat synthesis-related genes ( SCD‑1 ) were significantly decreased, providing a basis for in-depth analysis FGFR1 It provides key tools for the regulatory mechanisms of mammary epithelial cell proliferation, lactation synthesis and other biological behaviors.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

A portable method and device for monitoring sodium ions in breast milk, belonging to the field of lactation status feedback, is disclosed. The method is executed after a breast milk container is installed on the main unit and includes the following steps: breast milk container identification and batch parameter loading, posture detection and gating, dry self-calibration of the electrical channel, thermal pulse temperature response acquisition and thermodynamic virtual volume feature extraction, low-frequency / high-frequency electrical response acquisition and bubble interference feature extraction, optical scattering signal acquisition and fat / turbidity proxy feature extraction, and geometric boundary compensation, temperature compensation, milk fat shielding compensation, and sodium ion concentration inversion based on the multi-dimensional features. It can also perform nursing guidance push, result storage, and edge-cloud collaborative updates. The device includes a main unit and a breast milk container. The main unit contains a heating film, a temperature probe, an optical turbidity detection module, and an electrode detection module connected to a four-electrode structure. This solution can improve the accuracy, stability, and repeatability of breast milk sodium ion detection in a home setting.

The invention relates to the technical field of dairy product processing, in particular to a double-fat double-protein synergistic dairy product and a preparation method thereof. According to the preparation method, a ''whey protein (WP)-casein phosphopeptide (CPP)'' double-protein system and a ''milk fat (MF)-medium chain triglyceride (MCT)'' double-fat system are constructed, and a four-step synergistic process of ''pretreatment, composite emulsification, gradient assembly and low-temperature curing'' is adopted, so that stable combination of a chemical interface of protein and fat is realized, high-temperature stability breakthrough is realized, and the preparation method has the advantages of high-temperature stability, high-temperature stability and high-temperature stability. Meanwhile, high-pressure processing tolerance is improved, fat globule flocculation caused by high pressure is avoided, and long-term storage stability, texture and taste are guaranteed.

The present invention provides a method of feeding a ruminant animal, preferably a pregnant ruminant animal, using a phytogenic composition. By feeding the composition pre-partum, the colostrum yield may be increased and colostrum quality is improved. Moreover, milk yield and milk fat percentage may be increased during lactation phase of the ruminant animal.

The utility model relates to the technical field of whey separation, in particular to a fat and whey separator in milk, which is characterized in that when the fat and whey separator is used, milk is stirred by a stirring component, milk fat particles are more uniformly distributed in the milk by stirring, subsequent separation is facilitated, and then a liquid conveying component is started; therefore, the stirred milk is filtered through one station of the double-station filtering assembly and cleaned through the other station, continuous separation of fat and whey of the milk is kept while filtering and cleaning are achieved, and the separation efficiency is improved; comprising a bottom plate and a disc type centrifugal machine, the disc type centrifugal machine is installed at the top end of the bottom plate, the device further comprises a stirring assembly, a liquid conveying assembly and a double-station filtering assembly, the liquid conveying assembly and the stirring assembly are arranged at the top end of the bottom plate, and the input end of the liquid conveying assembly communicates with the output end of the stirring assembly; the output end of the liquid conveying assembly communicates with the input end of the double-station filtering assembly, and the output end of the double-station filtering assembly communicates with the input end of the disc type centrifugal machine.

The invention provides a rumen bypass folic acid additive, a preparation method and application thereof and a method for producing milk rich in folic acid, and particularly belongs to the technical field of rumen bypass additives. The rumen bypass folic acid additive comprises folic acid, cobalamin, silicon dioxide, lipase, palm fat, coconut oil and calcium stearate. The rumen bypass folic acid additive can reduce folic acid degradation by rumen microorganisms, can directly reach small intestines to be absorbed, improves the folic acid utilization rate of ruminants, specifically can improve the milk yield, milk protein, milk fat and folic acid content and yield of dairy cows, and can produce folic acid-rich cow milk; the feed can also increase the plasma glucose level and reduce the concentration of beta-hydroxybutyric acid, so that the energy metabolism state of the dairy cow is improved, the production performance of the dairy cow is improved, and economic benefits are brought to pastures.

The invention relates to the field of molecular biology, and discloses a haplotype marker for dairy cow milk production butter-fat percentage character breeding and application of the haplotype marker, the haplotype marker is composed of three SNP sites in a strong linkage imbalance state, and the three SNP sites are located in a Block1 haplotype block region of a 10 # chromosome NC037337.1 of a dairy cow ARS-UCD1.2 genome; the three SNP sites are as follows: SNP1: g.59680573 A > C, SNP2: g.59743173 G > C, and SNP3: g.59743414 C > T; the dominant haplotype of the haplotype marker is H2, and the allele combination of the haplotype marker is SNP1-A, SNP2-G and SNP3-C; the homozygous H2H2 of the dominant haplotype H2 is significantly related to the high milk fat percentage character of milk production of dairy cows. The bottleneck of the traditional technology is solved, and the breeding accuracy is remarkably improved; the screening detection technical scheme is stable and reliable and has a wide application range; the milk production quality of groups is improved, and the industrial economic value is enhanced.

The present application relates to a kind of fat powder for reducing weaning piglet dead rate and its preparation method and application, and the composite lipid in the preparation raw material of fat powder includes 1,3-di-oleic acid-2-palmitic acid triglyceride, 24 degrees palm oil, rice bran oil, coconut oil, linseed oil, caprylic triglyceride, capric acid triglyceride, alpha-lauric acid monoglyceride and tributyrin.The present application develops the fat acid composition close to pig milk fat structure, let piglet continue lactation period in weaning period to enable approach mainly with lipid, meet the compound energy demand of fast, medium and slow, avoid falling into energy potential difference;And the specific combination of medium and short chain fatty acids, both can improve the energy efficiency of weaning piglet, also have bacteriostatic disease characteristics, can improve the immunity of piglet, reduce the diarrhea rate.This composite lipid overcomes the problem such as piglet energy deficiency and indigestion caused by single raw material oil due to unsuitable lipid structure, can significantly reduce weaning stress, reduce the diarrhea rate and dead rate.

The present invention belongs to the fields of molecular biotechnology and molecular marker technology, and specifically relates to an SNP locus related to yak milk density and milk fat content and its application. The SNP locus is located at the 13,729,718th position of chromosome 5 of the yak reference genome Bosgu_v3.0 version with the Ensemble accession number ENSBGRG00000003042, and the mutated base is C or T. The present invention also provides the application of a reagent for detecting the above SNP locus in the auxiliary breeding for detecting yak milk density and milk fat content. This molecular marker can be applied to the auxiliary selection of yak milk density and milk fat content traits, and the detection method is rapid and accurate; by screening the genotypes of this SNP molecular marker, the accuracy and effectiveness of the selection of yak milk density and milk fat content traits can be accelerated, and the economic benefits of yak breeding can be improved.

The invention relates to the technical field of food, in particular to mascarpone cheese and a preparation method thereof, and the mascarpone cheese comprises the following raw materials in percentage by mass: 70-85% of milk fat, 5-15% of milk solid substance, 0.2-2% of compound stabilizer, 0.1-1% of acidity regulator and the balance of water, the mascarpone cheese disclosed by the invention adopts a whey-free process, the 100% utilization rate of the raw materials is realized, no waste treatment burden exists, the process is simple, the mascarpone cheese can be directly produced on a reproduced cheese production line, the production efficiency is improved, manpower, material resources and equipment modification cost are saved, and the mascarpone cheese is free of preservatives and long in shelf life through UHT sterilization and sterile hot filling. The stability in a long shelf life period is realized by compounding a stable system, and a finished product is rich in milk flavor, smooth and fine in texture and moderate in hardness, and shows good application performance when being used for preparing desserts such as tiramisu and the like.

A frying oil with butter flavor, comprising a vegetable oil and hydrolyzed milk fat and optional anhydrous milk fat. Also disclosed is a method of preparing the frying oil and the use of the frying oil.

The invention discloses a molecular marker for detecting the milk production character of cattle and an identification method, and belongs to the technical field of biology. The SNP A provided by the invention is g.8044024C / T, the SNP B provided by the invention is g.8081101 A / G, and the SNP C provided by the invention is g.8081205C / G. The molecular marker g.8044024C / T, g.8081101 A / G or / and g.8081205C / G is associated with the milk yield, the milk fat amount and the milk protein amount at a remarkable or extremely remarkable level (P = 0.02-2.00 E-16). By utilizing the SNP provided by the invention, early screening can be realized in the dairy cow breeding process, the breeding cost can be reduced, and the SNP has an important application prospect in dairy cow breeding.

Fat blend comprising (i) 20-65 wt % of a vegetable lipid source and (ii) 35-80 wt % of a milk fat composition comprising at least one bovine milk fat fraction, wherein the milk fat composition comprises, relative to total fatty acid moieties, 37-48 mol % of long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0) and wherein 28-43 mol % of all fatty acid moieties positioned on the sn-1,3 position of the glycerol backbone in said milk fat composition are long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0).

This invention discloses the application of Cordyceps sinensis mycelium fermentation culture in improving the immunity and milk quality of dairy cows, belonging to the field of animal husbandry technology. The Cordyceps sinensis mycelium fermentation culture is obtained by liquid fermentation of Cordyceps sinensis, followed by solid-liquid separation, collection of the liquid fraction, concentration, and spray drying. This invention applies the Cordyceps sinensis mycelium fermentation culture as a feed additive to lactating dairy cows, significantly improving key milk quality indicators, including milk fat percentage, milk protein percentage, lactose percentage, non-fat solids content, and milk somatic cell count. It also enhances serum immune indicators in lactating dairy cows, improving their immunity. This invention provides a novel and effective solution to the industry challenge of finding green and safe feed additives in animal husbandry, and has clear industrialization prospects.

The invention belongs to the technical field of experimental animal nutriology, and discloses a high-fat feed composition for mouse metabolic syndrome and a preparation process of the high-fat feed composition. The high-fat feed composition comprises casein VTF, DL-methionine, corn starch, maltodextrin, cane sugar, cellulose, anhydrous milk fat, corn oil, ethoxyquin, a mineral mixture S10001AIN-76A, calcium carbonate, a vitamin mixture 40060-D and cholesterol. The preparation process comprises the following steps: S1, pretreating the raw materials; S2, preparing the materials; S3, mixing the materials; S4, granulating; the high-fat feed provided by the invention can effectively improve the food consumption of mice and ensure the weight growth rate of the mice; and on the basis that the atherosclerosis animal model is stable, the time required for preparing the atherosclerosis mouse is shortened.

The invention provides milk fat rich in concentrated chain triglyceride as well as a preparation method and application of the milk fat. The method for preparing the milk fat rich in the concentrated chain triglyceride comprises the following steps: (1) completely melting anhydrous milk fat in a water bath, preserving heat in the water bath, growing crystal at the temperature of 20-30 DEG C, and carrying out crystal separation to obtain soft fat and stearin; and (2) carrying out single-stage short-path molecular distillation on the molten soft fat at 175-185 DEG C to obtain a heavy-phase component and a light-phase component, and the light-phase component is the milk fat rich in the concentrated-chain triglyceride. The method is simple to operate and low in cost, and the MCT-rich soft fat is obtained through crystal growing separation and then is subjected to short-path molecular distillation, so that MCT can be effectively enriched, and the flavor quality of the milk fat is improved.

The present application relates to the technical field of livestock breeding, and discloses a feeding method for increasing milk fat rate of lactating donkey. The feeding method for increasing milk fat rate of lactating donkey comprises the following steps: feeding lactating donkey with a special feeding stuff for increasing milk fat rate of lactating donkey with specific nutrient level and nutrient substance ratio; wherein the nutrient level of the feeding stuff for increasing milk fat rate of lactating donkey is: 12±0.5 MJ / kg of digestible energy, 120±6 g / kg of crude protein, and 60±5 g / kg of crude fat content; and the nutrient substance ratio is: (50-60):(40-50) of crude protein ratio, and 2.2±0.2 of NDF / NFC. The feeding method for increasing milk fat rate of lactating donkey provided by the present application can promote the synthesis of milk fat of lactating donkey and increase the milk fat rate of lactating donkey by combining the increase of lipid and NDF in the feeding stuff under the condition of appropriate protein level of the feeding stuff.

This invention relates to a dairy processing technology and dairy products based on continuous hydraulic cavitation. The invention provides a dairy processing technology based on continuous hydraulic cavitation, comprising the following steps: (1) feeding the emulsion into a hydraulic cavitation reactor for continuous hydraulic cavitation pretreatment; (2) cooling the pretreated emulsion to remove excess milk fat, completing the defatting process; (3) ultrafiltration membrane concentration: concentrating the defatted emulsion through an ultrafiltration membrane to obtain lactose-free skimmed milk; (4) drying the lactose-free skimmed milk. This invention uses hydraulic cavitation technology to pretreat milk raw materials, completing the defatting and sterilization steps, and achieving a reduction in the average particle size of protein molecules, lactose, and ash content under low-temperature conditions, solving the problems of complex processing procedures, low product yield, and unsatisfactory product quality in traditional production technologies.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

The invention belongs to the field of gene editing, and particularly relates to a method for regulating milk fat content. The expression of genes in fatty acid and cholesterol synthesis pathways of the livers of the animals in the lactation period is specifically regulated and controlled through a gene delivery technology, the expression of liver apolipoprotein B (ApoB) and the triglyceride and total cholesterol level in blood can be remarkably reduced, then the lipid content of milk is effectively reduced, meanwhile, the food intake of the animals is reduced, and the production cost is reduced. And the milk protein content is not influenced. According to the method, milk fat components are accurately controlled through maternal liver gene editing for the first time, a new strategy is provided for breeding healthy dairy animals, and the method has wide application prospects.

The invention provides application of phytosterol ester in preparation of a product for improving lactation performance of bactrian camels, and belongs to the field of bactrian camel breeding. It is found that the lactation performance of the bactrian camels can be remarkably improved by adding the phytosterol ester, especially the beta-sitosterol ester, in the feed, the milk yield of the bactrian camels is increased, and the camel milk fat content and the camel milk density are increased. The invention further provides a feed for improving the lactation performance of the bactrian camels and a method for improving the lactation performance of the bactrian camels, the milk yield of the bactrian camels can be effectively increased, and the quality of camel milk is improved.

The invention provides a special texture modifier for low-fatty acid emulsion and a gradient application process of the special texture modifier. The special texture modifier for the low-fatty acid emulsion is prepared by taking lemon peel residues as raw materials through a ball milling-hydration synergistic modification process. The gradient application process specifically comprises the following steps: adding the texture conditioning agent into the production process according to 0.7-0.8% of the mass of the finished yoghurt, wherein the addition mass ratio of the proportioning process to the filling process is 4: 1. By applying the special texture regulator, the whey precipitation rate of the low-fatty acid milk can be reduced to 35.89%, the sense milk fat feeling is improved, and the texture and flavor of the product are synchronously improved. Gradient activation application of the lemon peel pomace in a dairy product system is achieved for the first time, traditional thickening agent compounding is replaced with physical modification, the problem that the utilization rate of agricultural product processing by-products is low is solved, the prepared regulator has ecological friendliness and economical efficiency, and a new scheme is provided for functional dairy product development.

The invention discloses a nutritional additive for livestock, which is prepared from thiamine and nicotinamide in a mass ratio of (1-2): (1-2), and is a brand-new nutritional additive capable of effectively relieving the industrial problems of sheep breast immunity, oxidative damage, lactation performance reduction and the like caused by heat stress. Under the optimized additive amount, by applying the nutritional additive, the functions of mammary gland cells can be remarkably improved, and the milk yield and the content of milk components such as milk fat, milk protein and lactose can be increased; according to the nutritional additive, a safe and stable daily ration formula can be established, and the residual risk of traditional chemical drugs or excessive vitamins is avoided.

The invention discloses a method for preparing hypoallergenic peanut milk by adding krill oil. The method comprises the following steps: (1) cleaning high-quality peanuts, and soaking the cleaned high-quality peanuts in water for 10 hours; (2) mixing the soaked peanuts with water, adding the krill oil into the mixture, and grinding into thick liquid; (3) performing high-pressure homogenization on the mixture obtained by grinding in the step (2) at 150MPa; (4) stirring the mixture subjected to high-pressure homogenization in the step (3) for 4 hours; 5) separating the mixture stirred in the step 4), and removing peanut residues to obtain peanut milk; (6) cooking the peanut milk obtained in the step (5), and cooling; 7, vitamin E is added into the cooled peanut milk obtained in the step 6, and nitrogen filling preservation is carried out. Krill oil-peanut oil milk fat film reconstruction is carried out through the high-pressure homogenization technology, it can be avoided that arachidonic acid and lipoxygenase generate proinflammatory substances, and the efficiency that EPA and DHA in krill oil phospholipid react with lipoxygenase to generate specific proinflammatory fading media can be improved.

To provide a milk-containing carbonated drink whose milk components have stable properties, which has a novel texture different from a carbonated milk drink while having a milk flavor.SOLUTION: A milk-containing carbonated drink contains polysaccharide including uronic acid as a constituent sugar, which has viscosity of 5.0-20 mPa s, 0.1-3.0 mass% of milk fat, a pH of 6.3-8.0, and a gas volume value of 1.7-2.4 GV. The present invention further provides a method for manufacturing the milk-containing carbonated drink and a method for imparting texture.SELECTED DRAWING: None

The present invention the novel ointment composition based on milkfat obtained from human breast milk and combined with a synergistically beneficial ratio of medium chain triglycerides to milk fat from human breast milk.

The invention provides a whipped milk base, and relates to the technical field of food processing. An emulsifier is not added into the raw materials of the whipped milk base, and the whipped milk base comprises the following raw materials: 45-65 parts of raw milk, 5-15 parts of milk fat, 1-5 parts of at least one of whey powder, milk powder and concentrated milk protein powder, 1-2 parts of dietary fibers and 0.1-0.5 part of a stabilizer. On the premise that no emulsifier is added, the whipped milk base can maintain good shelf life stability, the whipping performance of the whipped milk base during application can be effectively improved, the shelf life stability and the excellent whipping performance are both considered, and the quality requirements of freshly-made drinks for the appearance and durability of the milk foam are met. The fat content of the whipped milk base is 5.0-8.0%, the protein content is 3.0-5.0%, and the whipped milk base has natural milk fragrance, is light in blood sugar burden and free of drinking burden, and meets the requirements of modern consumers for healthy drinks.