49 results about "Bovine lactoferrin" patented technology

Use of lactoferrin or metal ion lactoferrin, preferably iron lactoferrin, preferably bovine lactoferrin, preferably iron bovine lactoferrin, or a metal ion functional variant or functional fragment thereof and at least one anti-tumour food factor selected from soy protein and vitamin D inhibits tumour formation or growth, maintains or improves one or both of the white blood cell count and red blood cell count, stimulates the immune system, and / or treats or prevents cancer. Dietary (foods or food supplements), nutraceutical or pharmaceutical compositions may be used.

The present invention relates to administration of milk fat or a milk fat analogue, optionally with at least one additional therapeutic factor, preferably lactoferrin or metal ion lactoferrin, preferably iron lactoferrin, preferably bovine lactoferrin, preferably iron bovine lactoferrin, or a metal ion functional variant or functional fragment thereof, to inhibit tumour formation or growth, maintain or improve one or more of the white blood cell count, the red blood cell count, or the myeloid cell count, reduce cachexia, mucositis, and anemia, stimulate the immune system and treat or prevent cancer and the symptoms of cancer and side-effects of cancer therapies. The methods and medicinal uses of the invention may be carried out by employing dietary (as foods or food supplements), nutraceutical or pharmaceutical compositions. Compositions useful in the methods of the invention are also provided.

The present invention relates to administration of milk fat or a milk fat analogue, optionally with at least one additional therapeutic factor, preferably lactoferrin or metal ion lactoferrin, preferably iron lactoferrin, preferably bovine lactoferrin, preferably iron bovine lactoferrin, or a metal ion functional variant or functional fragment thereof, to inhibit tumour formation or growth, maintain or improve one or more of the white blood cell count, the red blood cell count, or the myeloid cell count, reduce cachexia, mucositis, and anemia, stimulate the immune system and treat or prevent cancer and the symptoms of cancer and side-effects of cancer therapies. The methods and medicinal uses of the invention may be carried out by employing dietary (as foods or food supplements), nutraceutical or pharmaceutical compositions. Compositions useful in the methods of the invention are also provided.

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.

The invention provides a double-antibody sandwich colloidal gold detection test strip for human lactoferrin. The immune colloidal gold test strip comprises a water absorption pad, a base film, a gold-labeled pad and a sample pad which are connected in sequence, wherein a line C and a line T are arranged on the base film; the line C is coated with an antibody for goat anti-mouse IgG; the gold-labeled pad contains a colloidal gold labelled first monoclonal antibody; the line T is coated with a second monoclonal antibody; the first monoclonal antibody is secreted by a hybridoma cell strain with the collection number of CGMCC NO.10595; the second monoclonal antibody is secreted by a hybridoma cell strain with the collection number of CGMCC NO.10594. The double-antibody sandwich colloidal gold detection test strip can be used for detecting human lactoferrin by a double-antibody sandwich colloidal gold detection method, so that the sensitivity of 10ng / mL can be achieved, and no cross reaction for bovine lactoferrin and goat lactoferrin is caused; furthermore, no cross reaction for lysozyme transgenic cattle and human alpha-serum albumin transgenic cattle is caused.

The invention discloses a bovine lactoferrin antibacterial peptide fusion protein, a coding gene and application thereof. The bovine lactoferrin antibacterial peptide fusion protein is a protein in a) or b) as follows: a) the protein formed by fusing two or more than two polypeptides shown at 4th to 28th position from an amino terminal in a sequence 2 in a sequence table; and b) the a)-derived protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues in an amino acid sequence of the protein limited by the a) and has antibacterial activity. The invention also discloses the coding gene of the bovine lactoferrin antibacterial peptide fusion protein. The bovine lactoferrin antibacterial peptide fusion protein and plants cultivated by the coding gene can be utilized as bioreactors to produce antimicrobial peptides.

The invention discloses a method for representing activity relationships of antibacterial proteins or polypeptides. The method is characterized in that starting from 85 structural parameters and topological indexes of representation molecules, a characteristic descriptor comprising 166 amino acid systems is obtained through principle component analysis (PCA), namely a principle component score vector of structural and topological variable (VSTPV).. The VSTPV is applied to study of the quantitative structure-activity relationships (QSAR) of 101 antibacterial cationic peptides and 28 hydrolyzed bovine lactoferricins (BPPs), the models have better fitting and forecasting capabilities, and the cross validation related coefficients and related coefficients are above 0.70. The study shows that the VSTPV can well represent coded and uncoded amino acids and establish polypeptides with stronger forecasting capabilities and derivative QSAR models thereof.

The invention discloses a production method of pasture fresh milk, comprising the following steps: (1) selecting fresh cow milk; (2) checking and accepting; (3) degassing; (4) clarifying the cow milk; (5) performing standardization; (6) blending, preheating and homogenizing; (7) performing plate-type pasteurization; (8) cooling and homogenizing; (9) performing aseptic packaging. The high-quality fresh milk in an own pasture breeding base is adopted as a base material, so the raw material source is natural and safe; in a production process, before a low temperature heat treatment sterilizing technology is adopted, bovine lactoferricin and a protein protectant are blended for the cow milk, so that the raw material protein heat resistance can be improved, denaturation by heating is not easy to cause, and meanwhile the mouthfeel and flavor of the raw material are improved; the bovine lactoferricin and the protein protectant further have the health efficacy of resisting bacteria and a synergistic effect, destroy nutrient components of the milk at a minimum level, have multiple bactericidal and antibacterial effects, and prolong the shelf life of the milk.

The invention belongs to the field of antibody engineering and immunoassay, and particularly relates to a competition law-based nanometer immunometric chromatographic detection card for lactoferrin inmilk. The nanometer immunometric chromatographicdetection card comprises a sample pad, a nanometer gold label pad, a nitrocellulose membrane (NC membrane), a water absorbent pad and a plastic card holder. The NC membrane is provided with a C line and a T line; the C line is sprayed with a goat anti-mouse IgG antibody; and the T line is coated with a bovine lactoferrin antigen. The gold label padcontains a nanometer gold-labeled murine monoclonal IgG antibody. Through the competition law-based nanometer immunometric chromatographic detection card, the lactoferrin in the milk can be rapidly detected, the detection sensitivity is 2.4 ng / mL, no cross reaction occurs among the main casein, serum albumin and skim milk in the milk; and the established detection method is convenient and quick and can be directly used for the rapid detection of lactoferrin contents in dairy products.

The invention discloses cat food for improving cat intestinal flora environment. The formula of the cat food comprises the following components in mass percentage: 14-18% of fresh beef, 2-6% of dehydrated beef, 6-10% of fresh pork, 2-6% of fresh pork liver, 2-6% of fresh pork liver, 2-6% of fresh beef tripe, 3-4% of beef fat, 3-4% of fresh beef kidney, 3-4% of fresh beef heart, 3-4% of dehydratedpork, 2-6% of salmon, 2-6% of fresh eggs, 2-6% of chicken, 2-6% of dehydrated lamb, 3-4 % of dehydrated tuna, 3-4% of dehydrated sardines, 1-2% of fresh pork liver, 1-2% of fresh lamb liver, 0-2% of menhaden oil, 0-2% fresh of lamb tripe, 0-2% of beef cartilage, 8-12% of vegetables, 7-11% of a flavor and 3-6% of bovine lactoferrin. The formula is capable of well controlling the moisture of the catfood, avoiding the cat food from being too dry to affect the digestion, and avoiding the cat food from having high moisture content to be beneficial to bacterial reproduction. Through replacing a preservative by the bovine lactoferrin, the antibacterial spectrum is broad, the shelf life can be prolonged, and the beneficial intestinal flora of cats are not affected after consumption.

The invention discloses an enzyme-linked immuno kit and a method for detecting bovine lactoferrin by using same, relating to a kit and a method for detecting bovine lactoferrin by using the kit. The invention solves the problems of the existing method for detecting bovine lactoferrin that the detection cost is higher, and the operation is complicated. The enzyme-linked immuno kit comprises an enzyme-linked plate, bovine lactoferrin standard substance, sample diluent, detection solution, substrate solution, cleaning solution and stop solution; the detection method mainly comprises the steps ofpreparing solution, combining an antibody on the enzyme-linked plate, closing the enzyme-linked plate, fixing the standard substance and the sample to be detected on the enzyme-linked plate, detectinglight absorption value by a microplate reader, making a standard curve according to detection results, and then calculating the content of the ovine lactoferrin serous of the sample to be detected. The method adopts a working solution manner, thus having low cost; and the method for detecting bovine lactoferrin by using the enzyme-linked immuno kit is simple in operation.

The invention provides a bovine lactoferrin quantitative detection kit. The bovine lactoferrin quantitative detection kit mainly comprises a bovine lactoferrin specific peptidase (amino acid sequence being as follows: LRPVAAEIYGTK), an isotope- labeled ovine lactoferrin specific peptidase (amino acid sequence being as follows: LRPV*AAEI*YGTK, wherein I* and L* are carbon-nitrogen isotope-labeled amino acid) and an isotope- labeled ovine lactoferrin internal standard (amino acid sequence being as follows: GRDPYKLRPV*AAEI*YGTKESPQTHY, wherein I* and L* are carbon-nitrogen isotope-labeled amino acid). During a using process, the bovine lactoferrin quantitative detection kit has the advantages of being simple in sample pre-treatment, high in sensitivity, strong in specificity, low in detection cost and the like, is suitable for rapid detection of large-scale samples, and can be used for satisfying accurate quantification of the bovine lactoferrin in various dairy products.

The invention relates to an expression vector and a preparation method and application thereof, in particular to a multi-copy integrated expression vector and a preparation method and application thereof in expression of bovine lactoferrin, and belongs to the technical field of gene engineering. The nucleotide sequence of the multi-copy integrated expression vector is expressed as SEQ ID No.1. In the multi-copy integrated expression vector, YIPlac204 plasmid is used as a skeleton, PGK is used as a promoter, G418 resistance is used as a selection marker, and rDNA is used as an integrated site; and the vector is a yeast multi-copy integrated expression vector suitable for industrialized production.

The present invention relates to administration of metal ion-saturated lactoferrin, preferably bovine lactoferrin, preferably iron-saturated bovine lactoferrin, or a metal ion-saturated functional variant or fragment thereof to inhibit tumour formation or growth, maintain or improve one or both of the white blood cell count and red blood cell count, stimulate the immune system and treat or prevent cancer. The methods and medicinal uses of the invention may be carried out by employing dietary (as foods or food supplements), nutraceutical or pharmaceutical compositions. Compositions useful in the methods of the invention are also provided.

Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing.

The invention belongs to the field of bio-genetic engineering and provides a shuttle plasmid and a method for efficiently expressing cecropin and lysozyme genes. The invention constructs an escherichia coli-bacillus subtilis shuttle plasmid suitable for being expressed in prokaryotes (escherichia coli, bacillus subtilis and bacillus sphaericus). Cecropins expressed in the prokaryotes does not have a toxic action on hosts. The shuttle plasmid can successfully express the fusion protein of various cecropins such as human lysozyme, bovine lactoferrin, and the like. 6His is introduced into the expression plasmid constructed by the invention, which is convenient to purify, and the purified fusion protein identifies and correctly cut enzyme cutting sites among all fusion peptide segments through lysostaphin so as to release all functional peptides and enable the functional peptides to recover the activity; and moreover, enzyme separation and cutting are also saved, thus the method can be effectively, simply and conveniently used. The shuttle plasmid also has a certain common availability, and all suitable genes can be cloned and expressed.

The invention relates to a liquid chromatography mass spectrometry detection method of lactoferrin. The method comprises the following steps: respectively carrying out trypsin enzyme enzymolysis treatment on a to-be-detected sample and a lactoferrin standard substance to prepare to-be-detected sample probational liquid containing a specific peptide fragment and a lactoferrin standard working fluidtreatment test solution containing the specific peptide fragment; preparing specific peptide fragment standard working fluid by using a specific peptide fragment standard substance; carrying out highperformance liquid chromatography mass spectrometry detection. According to the liquid chromatography mass spectrometry determination method of the lactoferrin, disclosed by the invention, the content of non-denatured and thermally-denatured bovine lactoferrin can be determined by using a specific peptide fragment external standard method to quantify, adopting a lactoferrin standard and using enzymolysis to control an enzymolysis coefficient.

A skin whitening emulsion is prepared from, by weight parts, 6-13 parts of white poria extract, 3-5 parts of glyceryl monostearate, 4-8 parts of sodium citrate, 2-5 parts of tea seed oil, 3-6 parts of embryo extract, 0.6-3.5 parts of penetrant, 0.02-0.06 part of epidermal growth factor, 3-8 parts of mung bean flour, 3-6 parts of surfactant, 0.2-0.5 part of preservative, 12-20 parts of eicosapentaenoic acid, 10-17 parts of super absorbent resin, 14-22 parts of tea polyphenols, 2-4 parts of glycerol monostearate, 0.2-0.4 part of potassium chloride, 2-4 parts of bovine lactoferrin and 3-8 parts of pearl. The skin whitening emulsion has the advantages of being capable of comprehensively restoring various kinds of skin damage, reducing melanin accumulation and having very good brightening and moisturizing effects.

The invention provides a cow milk immunoglobulin (IgG) double-antibody sandwich colloidal gold immunochromatographic test strip, a preparation method, a kit and a detection method. The colloidal goldimmunochromatography test strip is characterized by comprising a bottom plate, and a water absorption pad, a chromatographic membrane, a conjugate release pad and a sample pad which are arranged on the PVC bottom plate in an overlapping manner. The conjugate release pad is coated with a colloidal gold labeled bovine lactoferrin antibody a compound, the chromatographic membrane is provided with a detection line and a quality control line, the detection line is coated with an anti-bovine lactoferrin antibody b, and the quality control line is coated with goat anti-mouse IgG. The invention also provides a preparation method of the chromatographic test strip, a kit comprising the reagent strip, and a detection method of the kit. According to the technical scheme, the kit is prepared based on the principles of double-antibody sandwich colloidal gold color development and gold-labeled immunoassay analyzer quantification, and can be used for rapidly and quantitatively detecting the content ofbovine lactoferrin (IgG).

The invention provides a soluble granule for promoting calcium absorption and improving immunity, which is characterized by comprising the following raw materials: 100-150 mg of bovine lactoferrin, 2.5-3.125 g of casein hydrolysate, 0.034-12 g of sweeteners, 150-165 mg of sour additives, 540-660 mg of calcium salt, and 100-150 mg of edible solid essence. The preparation method comprises: well mixing the raw materials according to the weight contents, sieving the raw materials to obtain the soluble granules. The beneficial effect is that the soluble granule has good effects on delaying calcium phosphate deposition and improving passive and active absorption of calcium salt. The added sugar and lactoferrin has promotion effect on delaying calcium phosphate deposition by casein phosphoeptide, has very good recovery effect on spleen and thymus indexes of immunocompromised mice, and has very good recovery effect on sensitization due to immunosuppression.

The invention relates to a beef granule quick-frozen product with high oxidation resistance and strong antibacterial ability and a preparation method of the beef granule quick-frozen product. The beef granules are pickled by adopting naringenin, propolis and bovine lactoferrin before being rolled and kneaded. The naringenin, as a natural antioxidant, not only can improve the antioxidant activity of the beef granules, but also can effectively capture a harmful substance methylglyoxal (MGO) molecule brought by lipid oxidation or microbial growth in the production and processing process, prevent the MGO) molecule from inducing human protein modification, and enhance the safety of the beef granules. Moreover, the naringenin and the propolis can be covalently bound with the bovine lactoferrin to form a compound, so that the unique flavor of the beef granules is endowed, the antibacterial ability of the beef granules is improved by nearly 10 times, and the shelf life of the product is prolonged.

The invention provides a Chinese medicinal composition for enhancing immunity of children and application of the Chinese medicinal composition to preparation of a functional food for enhancing the immunity of the children and improving growth and development. The Chinese medicinal composition consists of the following components in mass ratio: 3-6 parts of yam, 1-3 parts of hawthorn and 1 part of bovine lactoferrin. The composition has rational compatibility, an obvious effect and effects of promoting digestion, enhancing the immunity, improving the growth and development.

The invention discloses a preparation method of a double-protein co-emulsified Pickering emulsion, and belongs to the technical field of food processing. The food-grade Pickering emulsion is preparedfrom soybean protein isolate nanoparticles and lactoferrin nanoparticles, so that the food-grade Pickering emulsion is more stable in the day and has excellent anti-aggregation stability. The preparation method provided by the invention is of great significance to design and manufacture of a formula of the protein-stabilized Pickering emulsion and even development of soybean protein products withcertain unique functions. In addition, 7s and 11s proteins in the soybean protein used in the method have good surface activity characteristics, so that the Pickering emulsion prepared by the method has good stability. The bovine lactoferrin is high in isoelectric point and is positively charged under a neutral condition, so that the bovine lactoferrin can interact with other biological macromolecules to improve the stability of the Pickering emulsion.

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.

The invention provides a binding buffer solution for extracting lactoferrin, a preparation method and application of the binding buffer solution and a detection method of bovine lactoferrin, and belongs to the technical field of protein detection. The binding buffer solution serves as an extracting solution of lactoferrin, the binding capacity of an affinity column on lactoferrin in a dairy product can be guaranteed, enrichment and detection of lactoferrin in a sample can be achieved, the extraction rate and the recovery rate of lactoferrin in the product are increased, the influence of a sample matrix on a target substance is overcome, and the detection accuracy is improved. The method can be applied to extraction of lactoferrin in different types of dairy products, quantitative detection of lactoferrin in different types of dairy products can be met, the recovery rate can reach 90% or above, the result is stable, and after elution, the peak shape is good, and impurity peaks are few.

The invention discloses a bovine lactoferricin peptide constitutive type yeast expression vector and a preparation method thereof. The expression vector is obtained by introducing a bovine lactoferricin peptide gene into pYES2 plasmids and utilizing a TPI (triosephosphate isomerase) promoter to substitute a GAL1 (galactokinase 1) promoter of the pYES2 plasmids. The pYES2-TPI-LfcinB recombinant plasmids constructed by the preparation method disclosed by the invention can effectively and stably secrete bovine lactoferricin peptide without using an inducer, so that production cost and production complexity are lowered.

The invention discloses essence with effects of skin whitening and freckle diluting. The essence is prepared from the following raw materials in parts by weight: 5-8 parts of aluminium silicate, 2-6 parts of benzoin, 3-5 parts of citric acid, 4-7 parts of methyl 4-hydroxybenzoate, 5-7 parts of stearic acid, 3-6 parts of bovine lactoferrin, 2-4 parts of Tween, 4-8 parts of thickening agent, 2-3.6 parts of emulgator, 3-6 parts of potato extract, 0.4-0.8 parts of collagen hydrolysate, 4-6 parts of coleus blumei, 5-8 parts of cucumber juice, 5-8 parts of glycerol, 4-10 parts of calabash extract, 2-6 parts of pearl and 3-7 parts of benzoic acid. The essence with effects of skin whitening and freckle diluting disclosed by the invention has the beneficial effects that the essence has a good skin whitening effect, removing the dull skin color, diluting freckles, restoring damaged cells and protecting the skin, and the essence has no side effect.

Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing.