49 results about "Bovine lactoferrin" patented technology

The present invention relates to administration of milk fat or a milk fat analogue, optionally with at least one additional therapeutic factor, preferably lactoferrin or metal ion lactoferrin, preferably iron lactoferrin, preferably bovine lactoferrin, preferably iron bovine lactoferrin, or a metal ion functional variant or functional fragment thereof, to inhibit tumour formation or growth, maintain or improve one or more of the white blood cell count, the red blood cell count, or the myeloid cell count, reduce cachexia, mucositis, and anemia, stimulate the immune system and treat or prevent cancer and the symptoms of cancer and side-effects of cancer therapies. The methods and medicinal uses of the invention may be carried out by employing dietary (as foods or food supplements), nutraceutical or pharmaceutical compositions. Compositions useful in the methods of the invention are also provided.

The invention discloses a bovine lactoferrin antibacterial peptide fusion protein, a coding gene and application thereof. The bovine lactoferrin antibacterial peptide fusion protein is a protein in a) or b) as follows: a) the protein formed by fusing two or more than two polypeptides shown at 4th to 28th position from an amino terminal in a sequence 2 in a sequence table; and b) the a)-derived protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues in an amino acid sequence of the protein limited by the a) and has antibacterial activity. The invention also discloses the coding gene of the bovine lactoferrin antibacterial peptide fusion protein. The bovine lactoferrin antibacterial peptide fusion protein and plants cultivated by the coding gene can be utilized as bioreactors to produce antimicrobial peptides.

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.

The invention discloses a bovine lactoferricin peptide constitutive type yeast expression vector and a preparation method thereof. The expression vector is obtained by introducing a bovine lactoferricin peptide gene into pYES2 plasmids and utilizing a TPI (triosephosphate isomerase) promoter to substitute a GAL1 (galactokinase 1) promoter of the pYES2 plasmids. The pYES2-TPI-LfcinB recombinant plasmids constructed by the preparation method disclosed by the invention can effectively and stably secrete bovine lactoferricin peptide without using an inducer, so that production cost and production complexity are lowered.

Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing.

The invention provides a preparation of bovine lactoferrin peptide chitosan nanoparticles, a preparation method and a freeze-dried powder, belonging to the technical field of nanomaterials. In the present invention, the low molecular weight chitosan powder is dissolved in the glacial acetic acid solution, and the pH of the solution is adjusted to 3-5 to obtain the low molecular weight chitosan solution; the bovine lactoferrin peptide solution is added to the low molecular weight chitosan solution to react to obtain a reaction Solution A; add sodium tripolyphosphate solution to reaction solution A and continue to react until a light blue opalescent dispersion is obtained. The bovine lactoferrin peptide-loaded chitosan nanoparticle prepared by the invention has good physicochemical characterization and in vitro antibacterial effect, has no cytotoxicity, and can be used as a promising antimicrobial peptide drug release system.