The invention discloses a tissue-culture rapid-propagation method of a double-happiness
cuckoo variety and a special culture medium thereof, belonging to the technical field of the
tissue culture andthe propagation of plants. The special culture medium comprises an adventitious
bud inducement culture medium, an inducement propagation culture medium, a clustered
seedling successive and sound-
seedling culture medium and a rooting culture medium, wherein the adventitious
bud inducement culture medium comprises 1 / 4MS, 3mg / L ZT, 0.5mg / L NAA, 30g / L
sugar and 5g / L agaragar; the inducement propagation culture medium comprises 1 / 4MS, 1mg / L ZT, 0.5mg / L NAA, 30g / L
sugar and 5g / L agaragar; the clustered
seedling successive and sound-seedling culture medium comprises 1 / 4MS, 0.5 mg / L ZT, 0.5mg / L NAA, 30g / L
sugar and 5g / L agaragar; the rooting culture medium comprises 1 / 4MS, 2mg / L IBA, 30g / L sugar and 5g / L agaragar; and pH values of all culture media are 5.4-5.6. The tissue-culture rapid-propagationmethod has a seedling
transplantation survival rate more than 90 percent and can achieve a
reproductive capacity higher than 0.1 million plants / year.