414 results about "Reproductive capacity" patented technology

The invention belongs to the technical field of facial mask materials and particularly relate to a preparation method of an aseptic-free non-woven facial mask. The non-woven facial mask is prepared through cooperation of a non-woven facial-mask base material made from polyester fiber, modified chitosan fiber and modified seaweed fiber with softeners composed of a glycerine ether quaternary ammonium salt softener and a diester quaternary ammonium salt softener with the mass ratio of 2:3 and anti-allergic agents composed of dendranthema indica and dipotassium glycyrrhizinate with the mass ratio of 4:1, and the non-woven facial mask is subjected to irradiation sterilization under conditions of normal pressure and temperature, oxidized gas media and low irradiation absorption dose. The preparation method has the advantages that the non-woven facial mask is high in moisture absorption and breathability and low in irritation, reproductive capacity of bacteria can be effectively reduced through irradiation sterilization of the non-woven facial mask, storage of the non-woven facial mask is benefited, and meanwhile, potential hazard of an aseptic to human skin is avoided.

The invention discloses a feed additive for improving the reproductive capacity of a boar. The feed additive comprises the following raw materials in percentage by weight: 40 to 50 percent of epimedium, 8 to 10 percent of cynomorium songaricum, 8 to 10 percent of codonopsis pilosula, 8 to 10 percent of angelica sinensis, 8 to 10 percent of medlar, 0.5 to 1 percent of vitamin A, 0.1 to 0.2 percent of vitamin D3, 1 to 4 percent of vitamin E, 0.1 to 0.3 percent of vitamin B2 and the balance of zeolite powder. A preparation method for the feed additive comprises the following steps of: mixing the epimedium, the cynomorium songaricum, the codonopsis pilosula, the angelica sinensis and the medlar; smashing a mixture to a particle size of between 25 and 40 microns; adding the vitamin A, the vitamin D3, the vitamin E, the vitamin B2 and the zeolite powder; and mixing uniformly. The feed additive prepared by the method has the characteristics of remarkable improvement on boar sexual desire, ejaculatory amount, sperm concentration, survival time and oestrum conception rate without any adverse effect, less additive amount, low cost and obvious effect.

The invention discloses a compound feed capable of enhancing reproductive capacity of a parent dace and aims at providing the compound feed capable of enhancing the reproductive capacity of the parent dace, the gonad index of the dace, the egg diameter, the egg laying amount, the fertilization rate of the parent dace, and the fry hatching-out rate. According to the technical scheme, the feed consists of basic ingredients and a Chinese herb medicine additive according to a mass ratio of (99.5-99.9):(0.1-0.5), wherein the basic ingredients include 20-30 parts of wheat, 4-6 parts of rice bran, 6-8 parts of vinasse, 20-30 parts of rapeseed meal, 15-20 parts of bean meal, 10-18 parts of a maize gluten feed, 2.5-4 parts of bean oil, 1 part of monocalcium phosphate, 1 part of bentonite, 0.5 part of multi-vitamin and 1.0 part of complex mineral matter; and the Chinese herb medicine additive consists of: 2-5 parts of saffron, 8-20 parts of glossy privet fruits, 5-20 parts of leonurus heterophyllus, 5-15 parts of officinal magnolia bark, 7-18 parts of Chinese magnoliavine fruit and 6-10 parts of liquorice. The compound feed belongs to the technical field of fish feeds.

The invention provides a breeding method of a new breed of high-yield fur sheep. Taihang fur sheep serve as female parents, and Tan sheep and Hu sheep serve as male parents for three-time grading hybridization and backcross; and the breed of the high-quality fur sheep with 'string patterns' is bred according to two-phase and three-phase phenotypic selection methods and the like. In the breeding method, the productivity and the fur quality of the Taihang fur sheep are improved by using the Hu sheep breed and the Tan sheep breed to be in hybridized and combined with the Taihang fur sheep and comprehensively utilizing the characteristics of high reproductive capacity, large wool yield and good fur quality of the Hu sheep and excellent fur patterns, good warming performance, no easy bonding, compact fur board, light weight and burliness of the Tan sheep on the basis of retaining the advantages of strong adaptability, crude feed resistance, strong disease resistance, easy management and the like of original breeds. Furs skinned from the high-yield fur sheep obtained by adopting the breeding method in the invention and killed at the age of about 30 days have excellent patterns such as the string patterns, big soft patterns and the like; moreover, the high-yield fur sheep have high lambing rate which is improved by 40% compared with the Taihang fur sheep.

The invention discloses a remediation method for heavy metal cadmium pollution of orchard soil based on galinsoga parviflora. The remediation method comprises the following steps: preparing galinsoga parviflora seedlings in a direct transplanting mode or a direct seeding mode; watering to guarantee the water-holding capacity of soil in a field and performing daily management on the galinsoga parviflora; performing harvesting treatment, i.e., harvesting the aboveground part of the galinsoga parviflora. The galinsoga parviflora has resistance and hyperaccumulation feature to cadmium heavy metal, the seedlings absorb and accumulate the cadmium heavy metal in the contaminated soil of the orchard, and the cadmium is transferred to the aboveground part to reduce the cadmium content in the orchard soil, so that the purpose of reducing the cadmium content in the contaminated soil of the orchard is achieved. In addition, the galinsoga parviflora has strong reproductive capacity, grows quickly and is shade-tolerant, the base part has strong branching capacity and can sprout after being harvested; the galinsoga parviflora can be planted almost all the year round in southern China and can grow in extremely barren land, the cost is low, the operability is high, and a new plant genetic resource is developed for phytoremediation of the heavy metal cadmium contaminated orchard soil.

The invention relates to a hormone spray agent for sow aphrodisiac, which comprises the following components in percentage by weight: 0.001-0.006% of swine ectohormone, 0-5% of cosolvent and balance of a water solution containing 55-85% of ethanol, wherein the swine ectohormone is androst-16-ene-3 alpha-ol or androst-16-ene-3-one. The spray agent of the invention has simple manufacturing, can effectively improve the reproductive capacity of a sow, and promotes the development of livestock breeding.

The invention discloses a Chinese herbal medicine feed additive for piglet fattening. The formula of the Chinese herbal medicine feed additive comprises the following components in parts by weight: 10-30 parts of acanthopanax root, 10-30 parts of codonopsis pilosula, 10-30 parts of fructus crataegi, 10-30 parts of fructus hordei germinatus, 10-30 parts of pericarpium citri reticulatae, 20-40 parts of rhizoma atractylodis, 40-60 parts of pine needles and 10-20 parts of glycyrrhiza uralensis. The Chinese herbal medicine feed additive can invigorate spleen to promote digestion, increase appetite of piglets, resolve dampness and tonify qi, improve the body immunity, and prevent diseases and help weight gaining, and since the piglets are fed by mixture of the feed additive with mixed feed, intestines and stomachs of piglets can be coordinated, the piglets are weaned as soon as possible, and the later reproductive capacity is greatly improved.

The invention relates to the technical field of aquaculture feedstuff, in particular to feedstuff additive premix material. The feedstuff additive comprises the following components according to the following weight portion: 150 to 450 portions of astaxanthin, 300 to 700 portions of dunaliella sallina, 150 to 400 portions of vitamin E, 300 to 600 portions of Chinese thorowax, 100 to 250 portions of canthaxanthin, 300 to 600 portions of antioxidant, and 150 to 1,000 portions of corn protein powder. The feedstuff additive has no innocuous and side residue, no drug resistance, environmental protection, and no adverse effect on the feedstuff palatability. The feedstuff additive can obviously improve the body color of fishes in the yellow and red color systems such as golden pompano, pseudobagrus fulvidraco, clarias fuscus, sea bream, large yellow croaker, and the like, can make the body color display natural yellow or red color, and can effectively improve the immunity, reproductive capacity and meat quality of cultivated fish. In addition, the feedstuff additive has stable raw material sources and a simple manufacturing process.

The invention discloses a tissue-culture rapid-propagation method of a double-happiness cuckoo variety and a special culture medium thereof, belonging to the technical field of the tissue culture andthe propagation of plants. The special culture medium comprises an adventitious bud inducement culture medium, an inducement propagation culture medium, a clustered seedling successive and sound-seedling culture medium and a rooting culture medium, wherein the adventitious bud inducement culture medium comprises 1/4MS, 3mg/L ZT, 0.5mg/L NAA, 30g/L sugar and 5g/L agaragar; the inducement propagation culture medium comprises 1/4MS, 1mg/L ZT, 0.5mg/L NAA, 30g/L sugar and 5g/L agaragar; the clustered seedling successive and sound-seedling culture medium comprises 1/4MS, 0.5 mg/L ZT, 0.5mg/L NAA, 30g/L sugar and 5g/L agaragar; the rooting culture medium comprises 1/4MS, 2mg/L IBA, 30g/L sugar and 5g/L agaragar; and pH values of all culture media are 5.4-5.6. The tissue-culture rapid-propagationmethod has a seedling transplantation survival rate more than 90 percent and can achieve a reproductive capacity higher than 0.1 million plants/year.

The invention relates to an artificial breeding technology for schizothorax grahami. The artificial breeding technology for the schizothorax grahami includes that cleaning and sterilizing a breeding pond; placing fish seeds; acclimatizing parent fishes and hastening parturition to spawn; incubating fish fries; feeding fodder; fishing and coming into the market. The artificial breeding technology for the schizothorax grahami is capable of effectively improving the problems of low survival rate, slow growth and low reproductive capacity of artificial schizothorax grahami breeding, improving the yield and economical benefit of the schizothorax grahami and promoting the development of the schizothorax grahami breeding industry.

The invention relates to a feed additive and a production method thereof. The feed additive comprises beta-carotene, VE, potassium chloride, magnesium sulfate, ferrous glycinate, yeast selenium, folicacid, vitamin B1, sodium sulfate and the like, and is a high-efficiency nutritious functional compound premix which is designed specially aiming at the problems troubling the pig industry, such as reduction of reproductive capacity and milking capacity of sows, heat stress and the like. The invention can effectively relieve the heat stress caused by climate and environment, prevent the occurrenceof sow agalactosis, improve the reproductive performance, prolong the breeding time and improve the survival rate of embryos, thereby increasing the litter size of sows, increasing the weight of newborn piglets, improving the survival rate and increasing the weight of weaning litter.

The invention provides a method for performing primary culture on canine umbilical cord-derived mesenchymal stem cells. The mesenchymal stem cells are derived from umbilical cords of neonatal dogs and are wide in source and readily available in materials, so that large-scale production and clinical application of the cells become possible in the later period. The culture method is easy to operate and high in repeatability, and a few parenchyma cells and pollution-free pure mesenchymal stem cells can be obtained. A collagenase digestion method used in the culture method is mild, cell damage is less, and lots of mesenchymal stem cells with high reproductive capacity can be obtained and can be used for subsequent cell line establishment, characteristics of stem cells, disease treatment and the like.

The invention relates to a composite antioxidant for improving reproductive capacity of a male animal, which belongs to the technical field of animal feed and comprises the following components by weight percent: 6.5-8.5% of food grade vitamin E, 11.0-15.5% of food grade vitamin C, 20.0-40.0% of food grade tea polyphenols, 4.0-6.0% of food grade thioctic acid and the balance of food grade corncobpowder which is taken as a carrier. The composite antioxidant can significantly reduce the content of nitrogen oxide free radicals and malonaldehyde in serum, improve the activity of SOD enzyme, significantly increase the number of sperms and the number of a grade sperms (the sperms with strong activity) in a male rat and improve the reproductive capacity of the male animal.

The invention relates to the field of stem cells and discloses a cryopreservation protection solution and a cryopreservation method for placenta amniotic mesenchymal stem cells. The cryopreservation protection solution for the placenta amniotic mesenchymal stem cells is prepared from 10-20 v/v% of DMSO, 30-80 v/v% of placenta amniotic mesenchymal stem cell conditioned media and 10-50 v/v% of fetal calf serums. After the cryopreservation protection solution is adopted for cryopreservation before resuscitation, the cell recovery rate is obviously higher than that of a conventional cryopreservation solution, and the cell reproductive capacity is superior to that of a conventional cell cryopreservation solution. According to the cryopreservation method, the placenta amniotic mesenchymal stem cells are digested and centrifuged, the placenta amniotic mesenchymal stem cell conditioned media are added for adjusting cell density, and the cells are cryopreserved after the cryopreservation protection solution with the same volume as the media is added. After the cells cryopreserved through the cryopreservation method resuscitate, the cell recovery rate is obviously higher than that of a conventional method, and the cell reproductive capacity is superior to that of a conventional cryopreservation method.

The invention discloses a regulating method of a WDR63 gene in osteogenic differentiation and odontogenicdifferentiation processes of a mesenchymal stem cell. The cell is replanted by virtue of cell culture, chromatin immunoprecipitation reaction and data analysis, plasmid construction and virus infection, western blotting analysis, cell reproductive capacity determination and alkaline phosphatase and alizarin red dyeing subcutaneously of a nude mouse, and the regulating effect of the WDR63 gene in osteogenic differentiation and odontogenic differentiation processes of the mesenchymal stem cell and the effect of promoting regeneration of tooth tissues are found. By virtue of the adjusting effect of gene activity and function of the mesenchymal stem cell by trimethylation of lysine on the fourth site of histone 3, the regulating effect of the WDR63 gene in osteogenic differentiation and odontogenicdifferentiation processes of the mesenchymal stem cell and the effect of the WDR63 gene in promoting regeneration of tooth tissues, the WDR63 gene obtained probably has a promoting effect on osteogenic differentiation of stem cells from apical papilla.

The invention discloses a kit for detecting the reproductive capacity of sheep and a using method thereof. The kit is internally provided with primer nucleotide sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 in a sequence table, probe nucleotide sequences shown in SEQ ID NO:5 and SEQ ID NO:6 as well as respectively placed FTA (Fault Tree Analysis) card and relevant reagents. The using method of the kit provided by the invention comprises the following steps: adding the primer nucleotide sequences, probe nucleotide sequences and relevant reagents in the kit into the DNA (Deoxyribose Nucleic Acid) of the sheep to be detected; then carrying out PCR (Polymerase Chain Reaction) amplification; carrying out fusion curve analysis on an amplification product in a high-resolution fusion curve analyzer; and forecasting the lambing situation of the sheep to be detected according to G, A, T and C peaks in the fusion curve.

The invention provides a 1% compound premix for rabbits, belonging to the field of compound premixes. The compound premix of the invention contains vitamin A, vitamin D3, vitamin E, nicotinic acid, iron amino acid chelate, manganese amino acid chelate, zinc amino acid chelate, copper amino acid chelate, cobalt amino acid chelate, iodized salt, selenate, choline chloride, hydrolyzed animal protein and carriers. The compound premix of the invention is suitable for three rabbits with different growth phases, namely, leverets, breeding rabbits and growing rabbits, and can improve the survival rate of leverets, accelerate the growth of the growing rabbits and enhance the reproductive capacity of breeding rabbits. The compound premix for rabbits of the invention is safe for rabbits, low in cost and beneficial to popularization and production.

The invention discloses a medicine for lowering the concentration of uric acid in blood. The medicine provided by the invention and used for lowering the concentration of uric acid in blood has active ingredient of inactivated microorganisms for the expression of urate oxidase; urate oxidase comes from microorganisms, animals or plants. The invention enables engineering bacteria used for expressing urate oxidase to be embedded in micro-capsules and solves the problem of high treatment cost in oral micro-encapsulated urate oxidase. The invention inactivates the engineering bacteria used for expressing urate oxidase and enables the engineering bacteria to lose the reproductive capacity in the premise of maintaining the activity of urate oxidase so that the medicine has high safety. The medicine is orally taken for the treatment of hyperuricemia, so as to avoid the influence on the activity of urate oxidase caused by acid-base intestinal environment; besides, the balance among intestinal flora is not broken even a small amount of thalli is accidentally released from the micro-capsules.

The invention discloses a seed selection method for new double muscle rump strains of large Yorkshire pigs. The seed selection method comprises the following steps of 1, selecting individuals with obvious double muscle rump characters from a large Yorkshire pig swinery in a pig farm to form seed selection core group serving as a generation zero; 2, performing hybridization on male large Yorkshire pigs and female large Yorkshire pigs of the generation zero to produce swinery of the next generation, and selecting individuals with obvious double muscle rump characters from the swinery of the next generation to form a first-generation seed selection core group; 3, performing hybridization on standby male and female pigs of the first-generation seed selection large Yorkshire pigs to produce swinery of the next generation, and selecting individuals with obvious double muscle rump characters from the swinery of the next generation to be a second-generation seed selection core group; 4, repeating the step 3, and performing seed selection to obtain the new double muscle rump strains of the large Yorkshire pigs which have the appearance of double muscle rump characters on and can be stably genetic after 3-4 generations, wherein one generation is generated in one year. The new strains of the pigs have the advantages of high growth speed, high feed conversion rate, much lean, high meat quality, high reproductive capacity and the like.

The invention discloses a feed additive for improving the reproductive capacity of a sow. The feed additive comprises the following raw materials in percentage by weight: 45 to 50 percent of epimedium, 10 to 15 percent of leonurus, 5 to 10 percent of semen cuscutae, 5 to 10 percent of angelica sinensis, 3 to 5 percent of safflower, 0.3 to 0.5 percent of vitamin A, 1 to 2 percent of vitamin E, 0.5 to 0.8 percent of folic acid and the balance of zeolite powder. The invention also discloses a preparation method for the feed additive. The method comprises the following steps of: mixing the epimedium, the leonurus, the semen cuscutae, the angelica sinensis and the safflower; smashing a mixture to a particle size of between 25 and 40 microns; adding the vitamin A, the vitamin E, the folic acid and the zeolite powder; and mixing uniformly. The feed additive prepared by the method has the characteristics of less additive amount and quick response when in use, and can remarkably improve the oestrus rate and the conception rate of the sow, and has no toxic or side effect.

The invention discloses a lily planting method and belongs to the technical field of flower planting. The method includes the steps of selection and preparation of land, planting, field management, disease control and harvesting. The method is characterized in that four-year-old mother bulbs with developed roots and 3-5 bulblets are selected as seeds, the seeds are soaked in 50% 500 times dilution of carbendazim and agricultural streptomycin for 20 minutes and then is soaked in a 30% alcoholic solution for 3-5 minutes, and finally the seeds are washed thoroughly through sterile water, aired and seeded. Compared with existing lily planting methods, the lily planting method has the advantages that the four-year-old bulblets are selected as seeds, plants are mature, and the disease resistance and the reproductive capacity of the bulblets reach the best state, so the emergence rate and the quality can be improved beneficially.

Methods and kits are provided for manipulating and predicting the reproductive capacity of a female subject. The presence of endogenous neurotrophins and addition of exogenous neurotrophins, particularly BDNF, NT-4/5, and NGF, increase the reproductive capacity of a female patient by binding to receptors on the oocytes and stimulating maturation of the oocytes. Administration of antagonists act as a contraceptive because the antagonists prevent the neurotrophins from binding to oocyte receptors. Neurotrophins may also be used to stimulate the maturation of oocyte in vitro.

The invention provides a composite essential oil premixing agent special for breeding sows and a preparation technology thereof. The composite essential oil premixing agent is prepared from thymol, origanum oil, eugenol, cinnamyl aldehyde, eucalyptus oil, fried seeds of cowherb, extracts from angelica sinensis, soy isoflavones, mannan oligosaccharide, fructo-oligosaccharide, tea-leaf powder and silica. The technology of the composite essential oil premixing agent comprises the following steps that various kinds of essential oil are placed into a liquid mixer to be mixed to be uniform, the mixture is placed into a material soaking device, tea-leaf powder and silica are added to be soaked for 30 min, the mixture is added into a vacuum mixer, the other components are added, sealed mixing is performed for 15 min, and bagging sealing is performed to obtain the composite essential oil premixing agent. The composite essential oil premixing agent can achieve the effects of achieving sterilization, diminishing inflammation, promoting sow gastrointestinal tract ecological balance, improving the nutrient level and immunity, preventing sows from being infected by pathogenic bacteria before and after parturition and being capable of improving the quality of milk so as to promote growth and development of piglets and thereby improving the reproductive capacity of sows and economic benefits.

The invention provides a method for detecting sheep FecB gene polymorphism by use of the SNaPshot technology. The method is to conduct single nucleotide polymorphism detection on the 29382188bp site (NC_019463.1 on the sixth chromosome of sheep, based on sheep genomic sequence information version number Oar_v3.1, Dec, 2012) and judging whether the sheep FecB gene is homozygous or heterozygous according to the detection result. Compared with the traditional PCR-RFLP FecB gene detection method and other methods, the method provided by the invention is high in technical accuracy, quick in detection, low in cost, and easier in result identification, and can be used for automatically detecting the SNP site of the FecB gene. Through selection of the sheep FecB genetypes, GG homozygote individuals with high litter size character can be reserved to improve the sheep reproductive capacity, and the method has a potential application value in large-scale sheep breeding.

The invention relates to the field of livestock breeding and discloses a yak reproductive capacity improvement method. The method includes: breast feeding of newly born yak calves, foraging training and weaning training of yak calves at an age of three months or older, self-foraging training of weaned yak calves, feeding management of the weaned yak calves and female yak feeding management. The yak reproductive capacity improvement method has advantages that early weaning of the yak calves can be realized, adverse influences of factors such as stress, nutrition and lactation with calves on reproductive capacity of female yaks can be alleviated, postpartum body condition recovery of the female yaks can be promoted, and yearly reproduction can be realized.